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Mps1 (TTK) (NM_003318) Human Untagged Clone
CAT＃: SC118032
TTK (untagged)-Human TTK protein kinase (TTK), transcript variant 1
CNY 6,288.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT96; ESK; MPH1; MPS1; MPS1L1; PYT |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003318 edited
ATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTCCATAATGAACAAAGTG AGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGATGAACTAAGCTTGAATAAA ATTTCTGCTGATACTACAGATAACTCGGGAACTGTTAACCAAATTATGATGATGGCAAAC AACCCAGAGGACTGGTTGAGTTTGTTGCTCAAACTAGAGAAAAACAGTGTTCCGCTAAGT GATGCTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCAATTGAAGCGCTTCCCCCA GATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTGAGATTTGCTGAATTAAAA GCTATTCAAGAGCCAGATGATGCACGTGACTACTTTCAAATGGCCAGAGCAAACTGCAAG AAATTTGCTTTTGTTCATATATCTTTTGCACAATTTGAACTGTCACAAGGTAATGTCAAA AAAAGTAAACAACTTCTTCAAAAAGCTGTAGAACGTGGAGCAGTACCACTAGAAATGCTG GAAATTGCCCTGCGGAATTTAAACCTCCAAAAAAAGCAGCTGCTTTCAGAGGAGGAAAAG AAGAATTTATCAGCATCTACGGTATTAACTGCCCAAGAATCATTTTCCGGTTCACTTGGG CATTTACAGAATAGGAACAACAGTTGTGATTCCAGAGGACAGACTACTAAAGCCAGGTTT TTATATGGAGAGAACATGCCACCACAAGATGCAGAAATAGGTTACCGGAATTCATTGAGA CAAACTAACAAAACTAAACAGTCATGCCCATTTGGAAGAGTCCCAGTTAACCTTCTAAAT AGCCCAGATTGTGATGTGAAGACAGATGATTCAGTTGTACCTTGTTTTATGAAAAGACAA ACCTCTAGATCAGAATGCCGAGATTTGGTTGTGCCTGGATCTAAACCAAGTGGAAATGAT TCCTGTGAATTAAGAAATTTAAAGTCTGTTCAAAATAGTCATTTCAAGGAACCTCTGGTG TCAGATGAAAAGAGTTCTGAACTTATTATTACTGATTCAATAACCCTGAAGAATAAAACG GAATCAAGTCTTCTAGCTAAATTAGAAGAAACTAAAGAGTATCAAGAACCAGAGGTTCCA GAGAGTAACCAGAAACAGTGGCAATCTAAGAGAAAGTCAGAGTGTATTAACCAGAATCCT GCTGCATCTTCAAATCACTGGCAGATTCCGGAGTTAGCCCGAAAAGTTAATACAGAGCAG AAACATACCACTTTTGAGCAACCTGTCTTTTCAGTTTCAAAACAGTCACCACCAATATCA ACATCTAAATGGTTTGACCCAAAATCTATTTGTAAGACACCAAGCAGCAATACCTTGGAT GATTACATGAGCTGTTTTAGAACTCCAGTTGTAAAGAATGACTTTCCACCTGCTTGTCAG TTGTCAACACCTTATGGCCAACCTGCCTGTTTCCAGCAGCAACAGCATCAAATACTTGCC ACTCCACTTCAAAATTTACAGGTTTTAGCATCTTCTTCAGCAAATGAATGCATTTCGGTT AAAGGAAGAATTTATTCCATATTAAAGCAGATAGGAAGTGGAGGTTCAAGCAAGGTATTT CAGGTGTTAAATGAAAAGAAACAGATATATGCTATAAAATATGTGAACTTAGAAGAAGCA GATAACCAAACTCTTGATAGTTACCGGAACGAAATAGCTTATTTGAATAAACTACAACAA CACAGTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTACATCTACATG GTAATGGAGTGTGGAAATATTGATCTTAATAGTTGGCTTAAAAAGAAAAAATCCATTGAT CCATGGGAACGCAAGAGTTACTGGAAAAATATGTTAGAGGCAGTTCACACAATCCATCAA CATGGCATTGTTCACAGTGATCTTAAACCAGCTAACTTTCTGATAGTTGATGGAATGCTA AAGCTAATTGATTTTGGGATTGCAAACCAAATGCAACCAGATACAACAAGTGTTGTTAAA GATTCTCAGGTTGGCACAGTTAATTATATGCCACCAGAAGCAATCAAAGATATGTCTTCC TCCAGAGAGAATGGGAAATCTAAGTCAAAGATAAGCCCCAAAAGTGATGTTTGGTCCTTA GGATGTATTTTGTACTATATGACTTACGGGAAAACACCATTTCAGCAGATAATTAATCAG ATTTCTAAATTACATGCCATAATTGATCCTAATCATGAAATTGAATTTCCCGATATTCCA GAGAAAGATCTTCAAGATGTGTTAAAGTGTTGTTTAAAAAGGGACCCAAAACAGAGGATA TCCATTCCTGAGCTCCTGGCTCATCCATATGTTCAAATTCAAACTCATCCAGTTAACCAA ATGGCCAAGGGAACCACTGAAGAAATGAAATATGTTCTGGGCCAACTTGTTGGTCTGAAT TCTCCTAACTCCATTTTGAAAGCTGCTAAAACTTTATATGAACACTATAGTGGTGGTGAA AGTCATAATTCTTCATCCTCCAAGACTTTTGAAAAAAAAAGGGGAAAAAAATGA >OriGene 5' read for NM_003318 unedited
TTTCCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA ATCGGCACGAGGGTAGAAATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGAT TCCATAATGAACAAAGTGAGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGAT GAACTAAGCTTGAATAAAATTTCTGCTGATACTACAGATAACTCGGGAACTGTTAACCAA ATTATGATGATGGCAAACAACCCAGAGGACTGGTTGAGTTTGTTGCTCAAACTAGAGAAA AACAGTGTTCCGCTAAGTGATGCTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCA ATTGAAGCGCTTCCCCCAGATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTG AGATTTGCTGAATTAAAAGCTATTCAAGAGCCAGATGATGCACGTGACTACTTTCAAATG GCCAGAGCAAACTGCAAGAAATTTGCTTTTGTTCATATATCTTTTGCACAATTTGAACTG TCACAAGGTAATGTCAAAAAAAGTAAACAACTTCTTCAAAAAGCTGTAGAACGTGGAGCA GTACCACTAGAAATGCTGGNAATTGCCCCTGCGAATTANACCTNCAAAAAAGCAGCTGCT TTCAGAGGAGGAAAAGAAAGATTTATCAGCATCTACGGTATTAACTGCCCCAGATCATTT TCCGGGTCACTTGGGCATTTACAGAATANGAACAACAGTTGTGAATTCAGAGGACAGACT ACTAANGCCAGNTTTTATATGGGAGAGACATGCCANNCACAGAGCAGAAATAGGNTACCG GNAATTCATTTGAGACAACTANNCAAAACTAACAGTCATGCCCATTTTGGNNAGNAGTCC NAGNTNACCTT >OriGene 3' read for NM_003318 unedited
AATTGGGTCCCAAACTTTTTTACAAATTTTTTAAAACTACTTGCTATCCACCCACTATTC CAANAGTAAAATGATGTCAAGTAGATTACACAAGAGCATAAAACAGAGAAAACAAGTCTA CAATATATACAGTGCCATAAGTGGTTGCTATTTTTACAGTTTTCTTTTAAAGATAATCTA CTGAATTTTTTTTGCTGATAACACTTTATAGTGATGTTGTCTTCAAATGTAGATTTCCAC AGGGATTCAAGAGTATAACAGTCCAATATATTTTATAGGTGGTATCTGACATTACGAATA ACTGCAAATCATTTTTTTCCCCTTTTTTTTTCAAAAGTCTTGGAGGATGAAGAATTATGA CTTTCACCACCACTATAGTGTTCATATAAAGTTTTAGCAGCTTTCAAAATGGAGTTAGGA GAATTCAGACCAACAAGTTGGCCCAGAACATATTTCATTTCTTCAGTGGTTCCCTTGGCC ATTTGGTTAACTGGATGAGTTTGAATTTGAACATATGGATGAGCCAGGAGCTCAGGAATG GATATCCTCTGTTTTGGGTCCCTTTTTAAACAACACTTTAACACATCTTTGAGATCTTTC TCTGGAAATATCGGAAANTTCATTTCATGATTAGGATCAATTATGGCATGTAATTTAGAA ATCTGATTAATTATCTGCTGAAATGGTGTTTTCCNGTNAGTCATATAGTACAAAATACAT TCTAAGGACCAAACATCACTTTTTGGGGCTTATTCTTTGACTTTAGATTTTCCATTTCTC TCCTGGAGGAAGACATAATCTTTGATTGCTTCTGGTGGCATATAATAACTGGGCCAACCT GAAAAATTTTTACAACCCTTGTTGATCCTGTTGCATTTGGGTTGCATCCCCAAATCAATA GCTTTAGCATTCCTCAACTTCAGAAAGTACCTGGTTTAGAACCTGGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003318 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003318.3, NP_003309.2 |
| RefSeq Size | 2984 bp |
| RefSeq ORF | 2574 bp |
| Locus ID | 7272 |
| UniProt ID | P33981 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway |
| Gene Summary | This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009] Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Characterization of the Cellular and Antitumor Effects of MPI-0479605, a Small-Molecule Inhibitor of the Mitotic Kinase Mps1
,Keith D. Tardif, Aaron Rogers, Jared Cassiano, Bruce L. Roth, Daniel M. Cimbora, Rena McKinnon, Ashley Peterson, Thomas B. Douce, Rosann Robinson, Irene Dorweiler, Thaylon Davis, Mark A. Hess, Kirill Ostanin, Damon I. Papac, Vijay Baichwal, Ian McAlexander, J. Adam Willardsen, Michael Saunders, Hoarau Christophe, D. Vijay Kumar, Daniel A. Wettstein, Robert O. Carlson, and Brandi L. Williams,
Mol. Cancer Ther., Dec 2011; 10: 2267 - 2275.
[TTK]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200093 | TTK (Myc-DDK-tagged)-Human TTK protein kinase (TTK), transcript variant 1 |
CNY 6,280.00 |
|
| RC200093L1 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, Myc-DDK-tagged |
CNY 8,680.00 |
|
| RC200093L2 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, mGFP tagged |
CNY 8,680.00 |
|
| RC200093L3 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, Myc-DDK-tagged |
CNY 8,680.00 |
|
| RC200093L4 | Lenti ORF clone of Human TTK protein kinase (TTK), transcript variant 1, mGFP tagged |
CNY 8,680.00 |
|
| RG200093 | TTK (tGFP-tagged) - Human TTK protein kinase (TTK), transcript variant 1 |
CNY 7,880.00 |
|
| SC323539 | TTK (untagged)-Kinase deficient mutant (K553M) of Human TTK protein kinase (TTK) |
CNY 6,288.00 |
|
| SC323540 | TTK (untagged)-Kinase deficient mutant (K553M) of Human TTK protein kinase (TTK) |
CNY 6,288.00 |
