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RAD54 (RAD54L) (NM_003579) Human Untagged Clone
CAT＃: SC117863
RAD54L (untagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1
CNY 5,480.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hHR54; HR54; hRAD54; RAD54A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117863 sequence for NM_003579 edited (data generated by NextGen Sequencing)
ATGAGGAGGAGCTTGGCTCCCAGCCAGCTGGCCAAGAGAAAACCTGAAGGCAGGTCCTGT GATGATGAAGACTGGCAACCTGGCCTAGTGACTCCTAGGAAACGGAAATCCAGCAGTGAG ACCCAGATCCAGGAGTGTTTCCTGTCTCCTTTTCGGAAACCTTTGAGTCAGCTAACCAAT CAACCACCTTGTCTGGACAGCAGTCAGCATGAAGCATTTATTCGAAGCATTTTGTCAAAG CCTTTCAAAGTCCCCATTCCAAATTATCAAGGTCCTCTGGGCTCTCGAGCATTGGGCCTG AAAAGGGCTGGGGTCCGCCGGGCCCTCCATGACCCCCTGGAAAAAGATGCCTTGGTTCTG TATGAGCCTCCCCCGCTGAGCGCTCATGACCAGCTGAAGCTTGACAAGGAGAAACTCCCT GTCCATGTGGTTGTTGACCCTATTCTCAGTAAGGTTTTGCGGCCTCATCAGAGAGAGGGA GTGAAATTCCTGTGGGAGTGTGTCACCAGTCGGCGCATCCCTGGCAGCCATGGCTGCATC ATGGCTGATGAGATGGGCCTAGGAAAGACGCTGCAGTGCATCACATTGATGTGGACACTT TTACGCCAGAGTCCAGAGTGCAAGCCAGAAATTGACAAGGCAGTGGTGGTGTCGCCTTCC AGCCTGGTGAAGAACTGGTACAATGAGGTTGGGAAATGGCTCGGAGGGAGGATCCAACCT CTGGCCATCGATGGAGGATCTAAGGATGAAATAGACCAAAAGCTGGAAGGATTCATGAAC CAGCGTGGAGCCAGGGTGTCTTCTCCCATCCTCATCATTTCCTATGAGACCTTCCGCCTT CATGTTGGAGTCCTCCAGAAAGGAAGTGTTGGTCTGGTCATATGTGACGAGGGACACAGG CTCAAGAACTCTGAGAATCAGACTTACCAAGCCCTGGACAGCTTGAACACCAGCCGGCGG GTGCTCATCTCCGGAACTCCCATCCAGAATGATCTGCTTGAGTATTTCAGCTTGGTACAT TTTGTTAATTCCGGCATCCTAGGGACTGCCCATGAATTCAAGAAGCATTTTGAATTGCCA ATTTTGAAGGGTCGAGACGCTGCTGCTAGTGAGGCAGACAGGCAGCTAGGAGAGGAGCGG CTGCGGGAGCTCACCAGCATTGTGAATAGATGCCTGATACGGAGGACTTCTGATATCCTT TCTAAATATCTGCCTGTGAAGATTGAGCAGGTCGTTTGTTGTAGGCTGACACCCCTTCAG ACTGAGTTATACAAGAGGTTTCTGAGACAAGCCAAACCGGCAGAAGAATTGCTTGAGGGC AAGATGAGTGTGTCTTCCCTTTCTTCCATCACCTCGCTAAAGAAGCTTTGTAATCATCCA GCTCTAATCTATGATAAGTGTGTGGAAGAGGAGGATGGCTTTGTGGGTGCCTTGGACCTC TTCCCTCCTGGTTACAGCTCTAAGGCCCTGGAGCCCCAGCTGTCAGGTAAGATGCTGGTC CTGGATTATATTCTGGCGGTGACCCGAAGCCGTAGCAGTGACAAAGTAGTGCTGGTGTCG AATTACACCCAGACTTTGGATCTCTTTGAGAAGCTGTGCCGTGCCCGAAGGTACTTATAC GTCCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAGGTTGTAGAACGCTTCAAT AGTCCATCGAGCCCTGACTTTGTCTTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGCCTC AATCTCATTGGGGCTAACCGGCTGGTCATGTTTGACCCTGACTGGAACCCAGCCAATGAT GAACAAGCCATGGCCCGGGTCTGGCGAGATGGTCAAAAGAAGACTTGCTATATCTACCGC CTGCTGTCTGCAGGGACCATTGAGGAGAAGATCTTCCAGCGTCAGAGCCACAAGAAGGCA CTGAGCAGCTGTGTGGTGGATGAGGAGCAGGATGTAGAGCGCCACTTCTCTCTGGGCGAG TTGAAGGAGCTGTTTATCCTGGATGAAGCTAGCCTCAGTGACACACATGACAGGTTGCAC TGCCGACGTTGTGTCAACAGCCGTCAGATCCGGCCACCCCCTGATGGTTCTGACTGCACT TCAGACCTGGCAGGGTGGAACCACTGCACTGATAAGTGGGGGCTCCGGGATGAGGTACTC CAGGCTGCCTGGGATGCTGCCTCCACTGCCATCACCTTCGTCTTCCACCAGCGTTCTCAT GAGGAGCAGCGGGGCCTCCGCTGA Clone variation with respect to NM_003579.3 >OriGene 5' read for NM_003579 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTCGTCTCGGCT TATTGGGGACGGCCACTCTCACAGTTTGGTTCCAAACACCAGTTCCTGGATGGATTCCCG CCATCCATGCCCCCTCTTTAATTAGCCGGTCCTCTCAATAATGTAGCAGCCCCCTCTACA GATTAGACCCTGGTCCTACACTCTTAGCCGCTGCCTGCTTTTGACCTTTGGCTCATGGGT ACTTGACGTTTTAAACTCCTAGGCCCAGGATGAGGAGGAGCTTGGCTCCCAGCCAGCTGG CCAAGAGAAAACCTGAAGGCAGGTCCTGTGATGATGAAGACTGGCAACCTGGCCTAGTGA CTCCTAGGAAACGGAAATCCAGCAGTGAGACCCAGATCCAGGAGTGTTTCCTGTCTCCTT TTCGGAAACCTTTGAGTCAGCTAACCAATCAACCACCTTGTCTGGACAGCAGTCAGCATG AAGCATTTATTCGAAGCATTTTGTCAAAGCCTTTCAAAGTCCCCATTCCAAATTATCAAG GTCCTCTGGGCTCTCGAGCATTGGGCCTGAAAAGGGCTGGGGTCCGCCGGGCCCTCCATG ACCCCCTGGAAAAAGATGCCTTGGTTCTGTATGAGCCTCCCCCGCTGAGCGCTCATGACC AGCTGAAGCTTGACAAGGAGAAACTCCCTGTCCATGTGGTTGTTGACCCTATTCTCAGTA AGGGTTTGCGGCCTCATCAGAGAGAGGGAGTGAAATTCCTGTGGGAGTGTGTCACCAGTC GGCGCATCCCTGGCAGCCATGGCTGCATCATGGCTGATGAGATGGGCCTANGAAAGACGC TGCAGTGCATCACATTGATGTGGACACNTTTACGCCAGAGTCCAGAGTGCAAGCANAAAT GACAGGCAGTGGTGGTGTCGCCTTCCACCTGTG >OriGene 3' read for NM_003579 unedited
GGGGGGTTTTGTNNTTTTTTTTTTTTATTTTTCTTTTTTTTTTCCTAACCTTTTTCTTTT TTTTAACCAAAAAGTTTTTCCTTATAAAATAAATTTGGGCAAACACCATGCAGCCCTTCT TGATGATCTTCTCCCACCACAACAAAATTCAACAGGCGCCCTGTGCGAGCAAGCGTCCCC CCTTCCCCCTACCTCCTCCCCTTTCAACACCTTCACCCCACACCCACCTCTTCCTCCCCC CGTATGCCTCCTTTGCTCCCTTTTCCCCCACCCCTCTCCCCTCACCCCCCAGGTTCCCTC GCACCTGACCGCCATCTCTCCCCTCGCTTTCCTTCTCCTTCCTTCCTCTCTCCCCTCCCT ATCCCCTCTTTTTTCTCCCCCCCCCCCCCCCCCCTGTTCTTTTTCCCTTCTTGCCTTACC TTCCCTCCTCCCCCTCCGTTCGTTTTTCTTCTCTTCCCCTCCTTCATTTCTCTCTCCCCT CCACTTTCCTCACCTCCCCCATCCTTCCTTCTCACCTCTTCCTACCCTTTACCTCTAAAC TATCTAACAACACACACTTTCTTCTCTCTCTCCCCACATATACCCAGATCACCCGTCCCC ACTACCCTTTCTTGTCGTTTCCTCTCCTCCCCCCCCCCCCCCCCCCCACACCCATCCCTC CTCCGCCCCTCTCTCCTCCTCTCTCTCTCCCCTCCTTCATTTCTCCTGCCACTCGCCCCA CCACTCCCACCTATCATCCCTCCTTATTNCACTACATACATTTTATAATTTCTTCCTCTC GCGCAACTTTCTCCCTACCTCCCCTTATCCTCCGATATCAATTCTTAATACCAACGCCTT ATGCAAACATTTATTTATATATTCCCACACTATCTCCCCGTCCATCCTCCTCTTTTTTCA CTCACTTCCATCTTTTTTTTCCCTCTCGTTGTCACCACCATTCTGTCCCCACCACTCTCC CCATTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003579 |
| Insert Size | 2820 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003579.2, NP_003570.1 |
| RefSeq Size | 3107 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 8438 |
| UniProt ID | Q92698 |
| Domains | SNF2_N, DEAD, helicase_C |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Homologous recombination |
| Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220046 | RAD54L (Myc-DDK-tagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1 |
CNY 5,472.00 |
|
| RC220046L1 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, Myc-DDK-tagged |
CNY 7,872.00 |
|
| RC220046L2 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC220046L3 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC220046L4 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG220046 | RAD54L (tGFP-tagged) - Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1 |
CNY 6,370.00 |
