EIF3D (NM_003753) Human Untagged Clone
CAT#: SC117807
EIF3D (untagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D)
CNY 4,088.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | eIF3-p66; eIF3-zeta; EIF3S7 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC117807 sequence for NM_003753 edited (data generated by NextGen Sequencing)
ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCG GTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGA AAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCC TCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGGATGAAAGTAGCTTC CAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGAATCGAATGAGATTT GCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTTCAACCTGCAG ATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCT TCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATG AAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAA TACTACGACAAAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGCCACTGCGGAGCATC AAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCCGCAAGCTGGCAAAA ACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAGCTGTACCCGC TCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAA GATGAAGGTAATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAAC CACAATTTCTCCCAGCAGTGCTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCA AACCCGTTTGTGGAGGACGACATGGATAAGAATGAAATCGCCTCTGTTGCGTACCGTTAC CGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCCGTTGTGAGCACGATGGCGTC ATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAATGAGTGGGAT TCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTG CTGGCTGGATCTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCC TCACGCCACGTCATCCTAGGCACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATC AACCTGAGCGTGGAGAATGCCTGGGGCATTTTACGCTGCGTCATTGACATCTGCATGAAG CTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACAAGCAGGTCATCCGTGTCTAC AGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGAGGAGGAAGAA GAGGAAGAAGAAGAGGAAGAAACTTAA Clone variation with respect to NM_003753.3 >OriGene 5' read for NM_003753 unedited
GGTCACATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCAAGAGTGCT GCTGCTAACGCGGTCCCCGGCACGCACCATCTGTTGCCATCCCGGCCGGCCGAGGCCATT GCAGATTTTGGAAGATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCT GGGGTCCCTGTGCGGTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAG GAGATCGGCTAGGAAAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACA CAAATAAGTACTCCTCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGG ATGAAAGTAGCTTCCAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGA ATCGAATGAGATTTGCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGC AGTTCAACCTGCAGATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGAC TGCAGAAAAAGTTCCAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGA AACCCCGAGACTCTTCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATT NTCCTCAGTTGATGAAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTT GTGGNGCCCTAGAATACTACGACANAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGC CACTGCGGAGCATCAAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCC CGCAGCTGGCANAACTCAGGNGAATGTGTTTGCCACTGATGCCATNCCTGCCACGCTGAT GAGCTGTACCCGCTCAGTGTATTCTGGGAT >OriGene 3' read for NM_003753 unedited
ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTGAGGGAAA GACTAAACAGATATATATTTTATTTCATCTGCTAAATGTCAGAGAGCAAGTTAGACACAC ATTCCACTAAGCATCAAAGGCCCTCGTAGTCTCGGTGGAAGGACAAACTCCAGCTCCACA TCACTGGTTTAAGTTTCTTCCTCTTCTTCTTCCTCTTCTTCCTCCTCCTCTTCCTCCTCA TCTTCATCAGAGCTGAAGGTGCCATCAGGGAGGCTGTAGACACGGATGACCTGCTTGTTG GGGTCCTTGAGGATGAGGTATTTGCCCTCCTCCAGCTTCATGCAGATGTCAATGACGCAG CGTAAAATGCCCCAGGCATTCTCCACGCTCAGGTTGATCTGGCTGGCAAACTCATTAGGC TTGAACTGCTGGGTGCCTAAGATGACGTGGCGTGAGGAGTCTTTCACGTGGTACCGAGAC ACATAACCAAGCTTGAGGTACTCAGATCCAGCCAGCAAAGCACAGCAGGTCCACCGGGCC AACTTGTAGCTGTTGTTCTTCAGCTCCGTGGCAATGACAGCCCCTCGCTGAGAGTCCAGC TTCTGACGCCAGTCAACGCCATTACAGTGCCTGGAATCCCACTCATTGAGTGTCTTGATG TTGATGAAGGACACTTCCCCGTTGGCTCCAGTCATGACGCCATCGTGCTCACAACGGACA ATAAGGTCAATATCATCTCCAAGCTTTCACCTGCGGTAACCGTACGCAACAGAAGCGATT TCATTCTTATCCATGTCGTCCTNCCAAACGGNTTTGGGTGGGGAAATTGTATCTTTCCTT CCCTTCTCAACACTGCTGGGAGAATTGTGGTGATGTAGGTTGCCTCATGGCAGTTGCC |
Restriction Sites | NotI-NotI |
ACCN | NM_003753 |
Insert Size | 2000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003753.2, NP_003744.1 |
RefSeq Size | 2169 bp |
RefSeq ORF | 1647 bp |
Locus ID | 8664 |
UniProt ID | O15371 |
Domains | eIF-3_zeta |
Gene Summary | Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203830 | EIF3D (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D) |
CNY 4,080.00 |
|
RC203830L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit D (EIF3D), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203830L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit D (EIF3D), mGFP tagged |
CNY 5,890.00 |
|
RG203830 | EIF3D (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit D (EIF3D) |
CNY 5,680.00 |
|
SC322358 | EIF3D (untagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D) |
CNY 4,088.00 |