EIF3D (NM_003753) Human Untagged Clone

CAT#: SC117807

EIF3D (untagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D)



  "NM_003753" in other vectors (5)

CNY 4,088.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
EIF3D mouse monoclonal antibody, clone OTI14D6 (formerly 14D6)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms eIF3-p66; eIF3-zeta; EIF3S7
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117807 sequence for NM_003753 edited (data generated by NextGen Sequencing)
ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCG
GTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGA
AAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCC
TCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGGATGAAAGTAGCTTC
CAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGAATCGAATGAGATTT
GCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTTCAACCTGCAG
ATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC
CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCT
TCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATG
AAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAA
TACTACGACAAAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGCCACTGCGGAGCATC
AAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCCGCAAGCTGGCAAAA
ACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAGCTGTACCCGC
TCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG
AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAA
GATGAAGGTAATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAAC
CACAATTTCTCCCAGCAGTGCTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCA
AACCCGTTTGTGGAGGACGACATGGATAAGAATGAAATCGCCTCTGTTGCGTACCGTTAC
CGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCCGTTGTGAGCACGATGGCGTC
ATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAATGAGTGGGAT
TCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC
ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTG
CTGGCTGGATCTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCC
TCACGCCACGTCATCCTAGGCACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATC
AACCTGAGCGTGGAGAATGCCTGGGGCATTTTACGCTGCGTCATTGACATCTGCATGAAG
CTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACAAGCAGGTCATCCGTGTCTAC
AGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGAGGAGGAAGAA
GAGGAAGAAGAAGAGGAAGAAACTTAA

Clone variation with respect to NM_003753.3
>OriGene 5' read for NM_003753 unedited
GGTCACATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCAAGAGTGCT
GCTGCTAACGCGGTCCCCGGCACGCACCATCTGTTGCCATCCCGGCCGGCCGAGGCCATT
GCAGATTTTGGAAGATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCT
GGGGTCCCTGTGCGGTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAG
GAGATCGGCTAGGAAAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACA
CAAATAAGTACTCCTCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGG
ATGAAAGTAGCTTCCAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGA
ATCGAATGAGATTTGCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGC
AGTTCAACCTGCAGATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGAC
TGCAGAAAAAGTTCCAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGA
AACCCCGAGACTCTTCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATT
NTCCTCAGTTGATGAAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTT
GTGGNGCCCTAGAATACTACGACANAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGC
CACTGCGGAGCATCAAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCC
CGCAGCTGGCANAACTCAGGNGAATGTGTTTGCCACTGATGCCATNCCTGCCACGCTGAT
GAGCTGTACCCGCTCAGTGTATTCTGGGAT
>OriGene 3' read for NM_003753 unedited
ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTGAGGGAAA
GACTAAACAGATATATATTTTATTTCATCTGCTAAATGTCAGAGAGCAAGTTAGACACAC
ATTCCACTAAGCATCAAAGGCCCTCGTAGTCTCGGTGGAAGGACAAACTCCAGCTCCACA
TCACTGGTTTAAGTTTCTTCCTCTTCTTCTTCCTCTTCTTCCTCCTCCTCTTCCTCCTCA
TCTTCATCAGAGCTGAAGGTGCCATCAGGGAGGCTGTAGACACGGATGACCTGCTTGTTG
GGGTCCTTGAGGATGAGGTATTTGCCCTCCTCCAGCTTCATGCAGATGTCAATGACGCAG
CGTAAAATGCCCCAGGCATTCTCCACGCTCAGGTTGATCTGGCTGGCAAACTCATTAGGC
TTGAACTGCTGGGTGCCTAAGATGACGTGGCGTGAGGAGTCTTTCACGTGGTACCGAGAC
ACATAACCAAGCTTGAGGTACTCAGATCCAGCCAGCAAAGCACAGCAGGTCCACCGGGCC
AACTTGTAGCTGTTGTTCTTCAGCTCCGTGGCAATGACAGCCCCTCGCTGAGAGTCCAGC
TTCTGACGCCAGTCAACGCCATTACAGTGCCTGGAATCCCACTCATTGAGTGTCTTGATG
TTGATGAAGGACACTTCCCCGTTGGCTCCAGTCATGACGCCATCGTGCTCACAACGGACA
ATAAGGTCAATATCATCTCCAAGCTTTCACCTGCGGTAACCGTACGCAACAGAAGCGATT
TCATTCTTATCCATGTCGTCCTNCCAAACGGNTTTGGGTGGGGAAATTGTATCTTTCCTT
CCCTTCTCAACACTGCTGGGAGAATTGTGGTGATGTAGGTTGCCTCATGGCAGTTGCC
Restriction Sites NotI-NotI     
ACCN NM_003753
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003753.2, NP_003744.1
RefSeq Size 2169 bp
RefSeq ORF 1647 bp
Locus ID 8664
UniProt ID O15371
Domains eIF-3_zeta
Gene Summary Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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