EIF3D (NM_003753) Human Tagged ORF Clone
CAT#: RC203830
EIF3D (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D)
ORF Plasmid: tGFP
"NM_003753" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 4,080.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | eIF3-p66; eIF3-zeta; EIF3S7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203830 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCGGTTCCCGAGC AGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGAAAGGTTGCAGACTGGACAGG AGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCCTCTCAGTTTGGTGGTGGAAGTCAATATGCT TATTTCCATGAGGAGGATGAAAGTAGCTTCCAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACC AGCGGAATCGAATGAGATTTGCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTT CAACCTGCAGATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCTTCAGTTGAAG TTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATGAAGATGCGCTACTTGGAAGT ATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAATACTACGACAAAGCCTTTGACCGCATCACC ACGAGGAGTGAGAAGCCACTGCGGAGCATCAAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTG TCATCCGCAAGCTGGCAAAAACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAG CTGTACCCGCTCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAAGATGAAGGTA ATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAACCACAATTTCTCCCAGCAGTG CTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCAAACCCGTTTGTGGAGGACGACATGGATAAG AATGAAATCGCCTCTGTTGCGTACCGTTACCGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCC GTTGTGAGCACGATGGCGTCATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAA TGAGTGGGATTCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTGCTGGCTGGAT CTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCCTCACGCCACGTCATCCTAGG CACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATCAACCTGAGCGTGGAGAATGCCTGGGGCATT TTACGCTGCGTCATTGACATCTGCATGAAGCTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACA AGCAGGTCATCCGTGTCTACAGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGA GGAGGAAGAAGAGGAAGAAGAAGAGGAAGAAACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203830 protein sequence
Red=Cloning site Green=Tags(s) MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYA YFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKF QKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRIT TRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDK RDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDK NEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAV IATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGI LRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEEEEEEEEET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_003753 |
ORF Size | 1644 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003753.4 |
RefSeq Size | 1949 bp |
RefSeq ORF | 1647 bp |
Locus ID | 8664 |
UniProt ID | O15371 |
Domains | eIF-3_zeta |
MW | 64 kDa |
Gene Summary | Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
The m6A reader YTHDF1 promotes ovarian cancer progression via augmenting EIF3C translation
,Liu, T;Wei, Q;Jin, J;Luo, Q;Liu, Y;Yang, Y;Cheng, C;Li, L;Pi, J;Si, Y;Xiao, H;Li, L;Rao, S;Wang, F;Yu, J;Yu, J;Zou, D;Yi, P;,
Nucleic Acids Res.
,PubMed ID 31996915
[EIF3D]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203830L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit D (EIF3D), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203830L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit D (EIF3D), mGFP tagged |
CNY 5,890.00 |
|
RG203830 | EIF3D (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit D (EIF3D) |
CNY 5,680.00 |
|
SC117807 | EIF3D (untagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D) |
CNY 4,088.00 |
|
SC322358 | EIF3D (untagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D) |
CNY 4,088.00 |