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Vesicle docking protein p115 (USO1) (NM_003715) Human Untagged Clone
CAT＃: SC117786
USO1 (untagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1)
CNY 10,576.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | P115; TAP; VDP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117786 sequence for NM_003715 edited (data generated by NextGen Sequencing)
ATGAATTTCCTCCGCGGGGTAATGGGGGGTCAGAGTGCCGGACCCCAGCACACAGAAGCC GAGACGATTCAAAAGCTTTGTGACAGAGTAGCTTCATCTACTTTATTGGATGATCGAAGA AATGCTGTTCGTGCTCTCAAATCATTATCTAAGAAATACCGCTTGGAAGTGGGTATACAA GCTATGGAACATCTTATTCATGTTTTACAAACAGATCGTTCAGATTCTGAAATAATAGGT TATGCTTTGGACACACTATATAATATAATATCTAATGAAGAAGAAGAAGAAGTAGAAGAA AATTCCACAAGACAGAGTGAAGATTTGGGAAGCCAATTTACAGAAATTTTCATTAAGCAG CAGGAAAATGTCACTCTTCTGTTATCTTTATTGGAGGAGTTTGATTTCCATGTCCGCTGG CCTGGTGTGAAGCTTCTTACTTCTCTTTTAAAACAACTAGGGCCTCAGGTGCAACAAATT ATTTTAGTCAGTCCTATGGGTGTTTCAAGATTGATGGACTTACTAGCGGATTCCAGGGAA GTTATACGTAATGATGGCGTCTTACTACTGCAGGCACTAACAAGAAGCAATGGTGCAATC CAGAAAATTGTTGCTTTTGAAAATGCTTTCGAGAGACTACTGGACATTATTTCAGAGGAG GGGAACAGTGATGGAGGTATAGTAGTTGAAGATTGTTTGATTTTGCTCCAAAACTTATTA AAAAACAACAACTCCAATCAAAATTTTTTTAAAGAAGGCTCATATATTCAACGTATGAAA CCTTGGTTTGAAGTTGGAGATGAAAATTCTGGCTGGTCTGCACAGAAAGTGACCAATCTA CATCTAATGCTACAGCTTGTTCGTGTATTGGTATCTCCCACCAACCCTCCTGGTGCTACC AGTAGCTGCCAGAAGGCTATGTTCCAGTGTGGGTTATTGCAGCAGCTTTGTACTATCCTA ATGGCTACTGGGGTTCCTGCTGATATCCTGACTGAGACCATAAATACTGTATCAGAAGTT ATTCGAGGCTGCCAAGTAAACCAAGACTACTTTGCATCTGTAAATGCACCTTCAAACCCA CCAAGACCGGCAATTGTAGTACTTCTCATGTCCATGGTTAATGAAAGGCAGCCATTTGTT TTGCGCTGTGCTGTTCTCTATTGTTTCCAGTGTTTCTTGTATAAAAACCAAAAAGGACAA GGAGAAATCGTGTCAACACTTTTACCTTCTACCATTGATGCAACAGGTAATTCAGTTTCA GCTGGCCAGTTATTATGTGGAGGTTTGTTTTCTACTGATTCACTTTCAAACTGGTGTGCT GCTGTGGCCCTTGCCCATGCGTTGCAAGAAAATGCCACCCAGAAAGAACAGTTGCTCAGG GTTCAACTTGCTACAAGTATTGGCAACCCTCCAGTTTCTTTACTTCAACAGTGCACCAAT ATTCTTTCACAGGGAAGCAAAATACAAACAAGAGTTGGATTATTAATGTTGCTTTGTACC TGGCTAAGCAATTGTCCCATTGCAGTAACGCATTTTCTTCACAATTCAGCCAATGTTCCA TTTCTTACAGGACAAATTGCAGAAAATCTTGGAGAAGAAGAGCAGTTGGTCCAAGGCTTA TGTGCCCTTTTGTTGGGCATTTCGATTTATTTCAATGATAACTCACTTGAGAGCTACATG AAAGAGAAGCTAAAACAACTGATTGAGAAGAGGATTGGCAAAGAGAATTTCATAGAGAAA CTAGGATTTATTAGCAAACATGAGTTGTATTCCAGAGCATCTCAGAAACCCCAGCCAAAC TTTCCCAGTCCAGAATACATGATATTTGATCATGAGTTTACGAAGCTGGTAAAAGAACTT GAAGGTGTTATAACTAAGGCTATTTATAAGTCCAGTGAAGAAGATAAAAAAGAAGAAGAG GTGAAAAAAACATTAGAACAGCATGACAATATTGTGACTCACTACAAAAATATGATTCGA GAGCAGGATCTCCAACTTGAGGAATTAAGGCAGCAGGTTTCTACATTAAAATGTCAAAAT GAACAGCTCCAGACGGCAGTCACACAGCAAGTATCACAGATCCAGCAGCACAAAGACCAG TATAATCTTCTTAAAATACAGCTAGGAAAAGACAATCAGCATCAAGGTTCTTACAGTGAG GGGGCTCAGATGAATGGCATTCAGCCAGAAGAAATTGGTAGATTGCGAGAAGAGATAGAA GAATTAAAACGTAATCAGGAACTTTTACAAAGCCAGCTGACTGAAAAGGACTCTATGATT GAAAATATGAAATCTTCCCAAACATCTGGCACAAATGAACAGTCTTCAGCAATAGTTTCA GCTAGAGATTCTGAACAAGTTGCAGAATTAAAACAGGAACTGGCAACTTTAAAGTCTCAG TTAAACTCACAATCTGTGGAGATCACCAAACTACAGACAGAAAAGCAGGAACTGTTACAG AAAACAGAAGCGTTTGCAAAATCAGTTGAGGTACAAGGAGAGACCGAGACTATAATAGCC ACCAAAACTACTGATGTAGAAGGAAGACTGTCAGCATTATTACAAGAGACCAAAGAGTTA AAGAATGAAATTAAAGCTCTGTCTGAGGAAAGAACTGCCATTAAAGAGCAGCTGGATTCA TCTAATAGTACCATTGCCATTTTACAAACTGAGAAAGACAAACTAGAGTTGGAAATTACA GATTCTAAAAAAGAACAAGATGATCTCTTGGTGCTCTTGGCCGATCAAGATCAGAAAATA CTGTCATTGAAGAATAAACTCAAGGATCTTGGTCATCCAGTTGAAGAAGAGGATGAACTT GAATCTGGAGACCAAGAGGATGAGGATGATGAAAGTGAAGATCCTGGCAAGGATCTAGAT CATATCTAG Clone variation with respect to NM_003715.2 254 t=>c >OriGene 5' read for NM_003715 unedited
CGGTTCAGATATTTGTATACGACTTACTATNAGGNNCGGCCGCGCAATTCGGCACGAGGC TGGAGTGGCCGCCGAGTTGGAGGCGGTGGTGGCAGCAGTAGGAGTGTGTAGAGTGCGGGA TTGGGGGCCAGGCCCTGCGGAGGGCGGGGGAAGTTGTCTTCTTTTTTTTCCGGAGGGGCC GGTAAACCTGGTGGCTGAACGGCAAGATGAATTTCCTCCGCGGGGTAATGGGGGGTCAGA GTGCCGGACCCCAGCACACAGAAGCCGAGACGATTCAAAAGCTTTGTGACAGAGTAGCTT CATCTACTTTATTGGATGATCGAAGAAATGCTGTTCGTGCTCTCAAATCATTATCTAAGA AATACCGCTTGGAAGTGGGTATACAAGCTATGGAACATCTTATTCATGTTTTACAAACAG ATCGTTCAGATTCTGAAATAATAGGTTATGCTTTGGACACACTATATAATATAATATCTA ATGAAGAAGAAGAAGAAGTAGAAGAAAATTCCACAAGACAGAGTGAAGATTTGGGAAGCC AATTTACAGAAATTTTCATTAAGCAGCAGGAAAATGTCACTCTTCTGTTATCTTTATTGG AGGAGTTTGATTTCCATGTCCGCTGGCCTGGTGTGAAGCTTCTTACTTCTCTTTTAAAAC AACTAGGGCCTCAGGTGCAACAAATTATTTTAGTCAGTCCTATGGGTGTTTCAAGATTGA TGGACTTACTAGCGGATTCCAGGGAAGTTATACGTAATGATGGCGTCTTACTACTGCAGG CACTAACAAGAAGCATGGTGCAATCCAGAANATTGNTGCTTTTGAAAATGCTNTCGAGAG ACTACTGGACATTATTTCAGAAGAGGGGAAACAGTGATGGAGGTATAGTAGNTGAAAGAT TGTTTGATTNTGCT >OriGene 3' read for NM_003715 unedited
CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTTTTTTTTTTTTT TAAGTCAACGTATCTATTTTTATTATGAAACATTAAATTTTGACACATTGCCTCATTTGC TTTTTTAAAATCTATTATCTGACTTAAACCTATTCAGCAAAAATGCCAATAAATTATATT AATCATACTTTGGGTCTTTTTAAAACTAGGAACATAATATGTTTTATGATAAACAATAAT ACTAAATCTGAGTTGTATGAACTGTTAACTTGAAATTTGTTTTAAATGTTTAGCTTAAAA CAAAAAGAAAACCAATCACATTAATACACTGTTGCAAAAGTTTCTCCGGAATGCCCTCCA CATCACTGTGTGTCAGCATCCTTCGGCTTCTTCACTGAGGTATGGAATGCAGCCATATGT AGGTGTCAAGGCACTCATTCTAAGCTGTCCTATCCTGCACATCTTAGCAATCACATTAGA TGGAGGGCTGATGATATGCACTAACTCCCAACCCAGGCTATCTTGCTTTTAAATAAACTA ATTTCTTTAAAAGAAAATCATCAACTAAGGACTCAAATGCTGAATTTAGAAATAATAATT CCAATTCAATACACATTCAATCAAATGTGTTAGTACTTAAAAGTCACAAATTTTGCAAAG TAAACAACGAATGAACTAAGGGCAATAGCATTGTCACCACATGCCTTAAAAGGTATATTT TTGGTGTTTGAAGCTCTTTGCAAATACATTTTGGGACAGTTCAGATGGATTAAATAAAAA ATGTGTGGTCTACATAAATCAGTTTTAAGGGTTTCAACATGGTGGCAACATTACAAAAAT ATCATAGCCTTAAAGTGAATGTTTCCAACTGGTTCTAAATCTTATTTTCCTGAAGGTAAC CAAGTTAAAGCCCATCTCAAATAAGAATAAACCCTATGTCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003715 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003715.1, NP_003706.1 |
| RefSeq Size | 3980 bp |
| RefSeq ORF | 2889 bp |
| Locus ID | 8615 |
| UniProt ID | O60763 |
| Domains | Uso1_p115_head, Uso1_p115_C |
| Gene Summary | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (2) lacks two exons in the central coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207614 | USO1 (Myc-DDK-tagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1) |
CNY 10,568.00 |
|
| RC207614L1 | Lenti-ORF clone of USO1 (Myc-DDK-tagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1) |
CNY 9,030.00 |
|
| RC207614L2 | Lenti-ORF clone of USO1 (mGFP-tagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1) |
CNY 9,030.00 |
|
| RC207614L3 | Lenti-ORF clone of USO1 (Myc-DDK-tagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1) |
CNY 9,030.00 |
|
| RC207614L4 | Lenti-ORF clone of USO1 (mGFP-tagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1) |
CNY 9,030.00 |
|
| RG207614 | USO1 (tGFP-tagged) - Human USO1 vesicle docking protein homolog (yeast) (USO1) |
CNY 7,890.00 |
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