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WAVE 1 (WASF1) (NM_003931) Human Untagged Clone
CAT＃: SC117699
WASF1 (untagged)-Human WAS protein family, member 1 (WASF1), transcript variant 1
CNY 4,168.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NEDALVS; SCAR1; WAVE; WAVE1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117699 sequence for NM_003931 edited (data generated by NextGen Sequencing)
ATGCCGCTAGTGAAAAGAAACATCGATCCTAGGCACTTGTGCCACACAGCACTGCCTAGA GGCATTAAGAATGAACTGGAATGTGTAACCAATATTTCCTTGGCAAATATAATTAGACAA CTAAGTAGCCTAAGTAAATATGCTGAAGATATATTTGGAGAATTATTCAATGAAGCACAT AGTTTTTCCTTCAGAGTCAACTCATTGCAAGAACGTGTGGACCGTTTATCTGTTAGTGTT ACACAGCTTGATCCAAAGGAAGAAGAATTGTCTTTGCAAGATATAACAATGAGGAAAGCT TTCCGAAGTTCTACAATTCAAGACCAGCAGCTTTTCGATCGCAAGACTTTGCCTATTCCA TTACAGGAGACGTACGATGTTTGTGAACAGCCTCCACCTCTCAATATACTCACTCCTTAT AGAGATGATGGTAAAGAAGGTCTGAAGTTTTATACCAATCCTTCGTATTTCTTTGATCTA TGGAAAGAAAAAATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGGAAGCAGAAG CAGAAAAATCTAGATCGTCCTCATGAACCAGAAAAAGTGCCAAGAGCACCTCATGACAGG CGGCGAGAATGGCAGAAGCTGGCCCAAGGTCCAGAGCTGGCTGAAGATGATGCTAATCTC TTACATAAGCATATTGAAGTTGCTAATGGCCCAGCCTCTCATTTTGAAACAAGACCTCAG ACATACGTGGATCATATGGATGGATCTTACTCACTTTCTGCCTTGCCATTTAGTCAGATG AGTGAGCTTCTGACTAGAGCTGAGGAAAGGGTATTAGTCAGACCACATGAACCACCTCCA CCTCCACCAATGCATGGAGCAGGAGATGCAAAACCGATACCCACCTGTATCAGTTCTGCT ACAGGTTTGATAGAAAATCGCCCTCAGTCACCAGCTACAGGCAGAACACCTGTGTTTGTG AGCCCCACTCCCCCACCTCCTCCACCACCTCTTCCATCTGCCTTGTCAACTTCCTCATTA AGAGCTTCAATGACTTCAACTCCTCCCCCTCCAGTACCTCCCCCACCTCCACCTCCAGCC ACTGCTTTGCAAGCTCCAGCAGTACCACCACCTCCAGCTCCTCTTCAGATTGCCCCTGGA GTTCTTCACCCAGCTCCTCCTCCAATTGCACCTCCTCTAGTACAGCCCTCTCCACCAGTA GCTAGAGCTGCCCCAGTATGTGAGACTGTACCAGTTCATCCACTCCCACAAGGTGAAGTT CAGGGGCTGCCTCCACCCCCACCACCGCCTCCTCTGCCTCCACCTGGCATTCGACCATCA TCACCTGTCACAGTTACAGCTCTTGCTCATCCTCCCTCTGGGCTACATCCAACTCCATCT ACTGCCCCAGGTCCCCATGTTCCATTAATGCCTCCATCTCCTCCATCACAAGTTATACCT GCTTCTGAGCCAAAGCGCCATCCATCAACCCTACCTGTAATCAGTGATGCCAGGAGTGTG CTACTGGAAGCAATACGAAAAGGTATTCAGCTACGCAAAGTAGAAGAGCAGCGTGAACAG GAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCATCCTGTCTCGCCGTATTGCT GTTGAATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGATTGGTTGGAGTAA Clone variation with respect to NM_003931.2 >OriGene 5' read for NM_003931 unedited
NGGGTCGTTTTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGCTAGCTTC GGTACTCTGACACCTTCTCTTGCACTTGCGGATGATGAACTGGAATAACGATGAAAGAAA GCACATCCGATCTCAACATTCACGTCCTGCCCTATAACCGATTAATTAATTGATCCCCAG CTAGACTAGTGTTGGAGAAATCAGCATGTTAAAACAACTGTTGATGATAGCTGTTGGAGT AAAGTTGCAGTGGAAGCTATGGCTGCAAAATCGTTAAAATCTTCAAGGTGAACTGGCACA AAGGTTAATCTCAAGATGCCGCTAGTGAAAAGAAACATCGATCCTAGGCACTTGTGCCAC ACAGCACTGCCTAGAGGCATTAAGAATGAACTGGAATGTGTAACCAATATTTCCTTGGCA AATATAATTAGACAACTAAGTAGCCTAAGTAAATATGCTGAAGATATATTTGGAGAATTA TTCAATGAAGCACATAGTTTTTCCTTCAGAGTCAACTCATTGCAAGAACGTGTGGACCGT TTATCTGTTAGTGTTACACAGCTTGATCCAAAGGAAGAAGAATTGTCTTTGCAAGATATA ACAATGAGGAAAGCTTTCCGAAGTTCTACAATTCAAGACCAGCAGCTTTTCGATCGCAAG ACTTTGCCTATTCCATTACAGGAGACGTACGATGTTTGTGAACAGCCTCCACCTCTCAAT ATACTCACTCCTTATAGAGATGATGGTAANAGAAGGTTCTGAAGTTNTATACCAATCCTT CGTATTTCTTTGATCTATGGAAAGAANNAATGTTGCAAGATACAGNAGGATAGAGGAAGG NAAAGAGGAAGCAGAAGCAGAAAATTCTAGATCGTCCTCATGAACCAGNANAAGTGCCAG AGCACCTC >OriGene 3' read for NM_003931 unedited
TGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAACTTTTAAAA TGAACCATTTATTAAATCAGACTGTTATTCTTAACAGTTATGTAAGTTACATGTATGTTT AAGTCAGAGTATTTCACATGGAAAAGTTTTTAACTCCTATAGGCAAGCAAAATCATATCA CACAATATATAAGTGGGAAGGGGATACTGCTAAACATTCAAATAAGGCAAGTATATAAAA ACAATAAAACAATAATGAAAAAATTCAAGCATTCCTTTAAGAGAATTCAACACTACAAGC TAAATGTACTTTCTGAGTGTATTCGTATAATCAAGGCAGTGTTTCTTCTTTTAAAACATC AGGAAATGGAATAAGGCTCATTAGTAGATACAGCTGCCCTCAAGATTTCAATTTCAGTTG CTTTCTTTAAATTAAAATTCACAAAGTACACAATTAAGATATATCAAAAAACTGAATCTG CTACTCTAACAACAGCTGTCCATGCTTAATACTTAGTGGTTTATTTGACCAAATTAGTCT TTTCAGGGGGAAAAAAAGATAAGCCACTGTAAAACATTCAGTTTGAAATGTATGCTAATA TTTTCCTTAAAAATCAAAAGTTATGGAGGAAAAGGGTCATTTATTATAAGGAAAAGAAAG CCAAACACTATAATGACCCAACATTTTCCAGGAACAAGCACCCCAAAGGACATTTGCATT CACCTTTGCAATATTATCAATGCCTTTTTCTTACTCCACCAATCTACTTCATCAAATTCT GAAACCTCTTCCGAAATACTATTTTCAACAGCTATACCGGTGAAACCAGGATGGTGGCCA CCATCGTCTCCAAAGGCCTCCATGCCTTACCTTCCTGTTCACCCTCGTTTTTTTATTTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003931 |
| Insert Size | 2620 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003931.2, NP_003922.1 |
| RefSeq Size | 3230 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 8936 |
| UniProt ID | Q92558 |
| Domains | WH2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Novel Role of Rac1/WAVE Signaling Mechanism in Regulation of the Epithelial Na+ Channel
,Alexey V. Karpushev, Vladislav Levchenko, Daria V. Ilatovskaya, Tengis S. Pavlov, and Alexander Staruschenko,
Hypertension, May 2011; 57: 996 - 1002
[WASF1]
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Rac1 Regulates Neuronal Polarization through the WAVE Complex
,Sabina Tahirovic, Farida Hellal, Dorothee Neukirchen, Robert Hindges, Boyan K. Garvalov, Kevin C. Flynn, Theresia E. Stradal, Anna Chrostek-Grashoff, Cord Brakebusch, and Frank Bradke,
J. Neurosci., May 2010; 30: 6930 - 6943
[WASF1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216977 | WASF1 (Myc-DDK-tagged)-Human WAS protein family, member 1 (WASF1), transcript variant 1 |
CNY 4,160.00 |
|
| RC216977L1 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, Myc-DDK-tagged |
CNY 6,560.00 |
|
| RC216977L2 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216977L3 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216977L4 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG216977 | WASF1 (tGFP-tagged) - Human WAS protein family, member 1 (WASF1), transcript variant 1 |
CNY 5,760.00 |
