D aspartate oxidase (DDO) (NM_004032) Human Untagged Clone
CAT#: SC117617
DDO (untagged)-Human D-aspartate oxidase (DDO), transcript variant 2
CNY 2,400.00
CNY 6,270.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DASOX; DDO-1; DDO-2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC117617 sequence for NM_004032 edited (data generated by NextGen Sequencing)
ATGAGACCAGCCAGGCACTGGGAAACAAGGTTTGGTGCCAGAGATTTTGGTGNNTTCCAA GACTGCTTTTTCAGAGACAGGCTCATGGACACAGCACGGATTGCAGTTGTCGGGGCAGGT GTGGTGGGGCTCTCCACGGCTGTGTGCATCTCCAAACTGGTGCCCCGATGCTCCGTTACC ATCATTTCAGACAAGTTTACTCCAGATACCACCAGTGATGTGGCAGCCGGAATGCTTATT CCTCMCACTTATCCAGATACACCCATTCACACGCAGAAGCAGTGGTTCAGAGAAACCTTT AATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTGGTGTTCATTTGGTA TCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAGAAGACCTGTGGGAA CTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAAGCAGACAGCTTGCA GGAGACTCAAAGATTTTCCCTGTAAGGGGCCAAGTCCTCCAAGTTCAGGCTCCCTGGGTG GAGCATTTTATCCGAGATGGCAGTGGGCTGACATATATTTATCCTGGTACATCCCATGTA ACCCTAGGTGGAACTAGGCAAAAAGGGGACTGGAATCTGTCCCCGGATGCAGAAAATAGC AGAGAGATTCTTTCCCGATGCTGTGTTCTGGAGCCCTCCCTCCACGGAGCCTGCAACATC AGGGAGAAGGTGGGCTTGAGGCCCTACAGGCCAGGCGTGCGACTGCAGACAGAGCTCCTT GCGCGAGATGGACAGAGGCTGCCTGTAGTCCACCACTATGGCCATGGGAGTGGGGGCATC TCAGTGCACTGGGGCACTGCTCTGGAGGCCGCCAGGCTGGTGAGCGAGTGTGTCCATGCC CTCAGGACCCCCATTCCCAAGTCAAACCTGTAG Clone variation with respect to NM_004032.2 53 g=>n;54 c=>n;245 a=>m;686 c=>t >OriGene 5' read for NM_004032 unedited
NGTTCAGGGAATTATGAAACCACGATTTCACTATAGGGCGGAACGCCAATTCGGCACGAG GCTGTCTCTCTAGGCATGAGACCAGCCAGGCACTGCGAAACAGGTTTGGTGCCAGAGATT TTGGTGGCTTCCAAGACTGCTTTTTCAGAGACAGGCTCATGGACACAGCACGGATTGCAG TTGTCGGGGCAGGTGTGGTGGGGCTCTCCACGGCTGTTTTTTTCTCCAAACTGGTGCCCC GATGCTCCGTTACCATCATTTCAGACAAGTTTACTCCAGATACCACCAGTGATGTGGCAG CCGGAATGCTTATTCCTCACACTTATCCAGATACACCCATTCACACGCAGAAGCAGTGGT TCAGAGAAACCTTTAATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTG GTGTTCATTTGGTATCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAG AAGACCTGTGGGAACTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAA GCAGACAGCTTGCAGGAGACTCAAAGATTTTCCCTGTAAGGGGCCAAGTCCTCCAAGTTC ACGCTCCCTGNGTGGAGCATTTTATCCGAGATGGCAGTGGGCTGACATATATTTATCCTG GTACATCCCATGTAACCCCTAGTGGAACTAGGCAAAAAGGGACTGGAATCTGTCCCCCNA TGCAGAAAATAGCAGAGAGATTCTCTCCCGATGCTGTGTTCTGGAGCCCTCCCTCCACGG AGCCTGNCACATCAGGNAGAANGTGGGGCTTGAAGCCCTACAGCCCANGCGTGCGACTGC AGACAGAGCTCCTTGCGCGAGATGGACAGAAGCTGCCCTGTATCCACCACTATGGCCATG GAAGTGGGGCAATTCACTGCACTGGGGCN >OriGene 3' read for NM_004032 unedited
TCCATGTTATTTTTTTTTTTAGTAATTATGTTGCTGACTTGGCTATAACCTAAGAGATCA CAGCAGCTCTGCAATTGACAGTGATTTAAATGTTTTCCATACATTATCTCCTCCTGTGGA TTTATAAGCCAAATGATATCAAAATCTCTATAGCTTCCAACATTCATATAAATCTGCATA GGTCCTTGGACATCAGTTCTAAATAAATTTTACTCTATAAGAAGTATTTTTAACAAAAAA TAGAGCTTTATGCCCTATGCCATTAATGCTGGACTTCCTAAGTAAGCCTACATGTTGCAT CATTTCTTTTGTTGTTGGTGTTTTTTTTAGAGGTGGGAACTGGCACCTCTAAAAAATGTT ACCCAGATTGCACTCAAACTCCTGGGCTCAACAATCCTCCTGCCTCAGCCTCTCAAGTAG CTGGGACTATAGGCATGCCACCGTGCTCAGCTTACATGTTACACCACTTCTAATGTTGAA AACAAAGAAATGTCTAATTAAACTTTCATGCAGTGGAAAAGTTATTTGAACCTGTTCTGT GCTTTGATCAACAGTCTCTCAGTCTCTTTGCTGTCATTTTATGTCATCTACAGGTTTGAC TTGGGAATGGGGGTCCTGAGGGCATGGACACACTCGCTCACCAGCCTGGCGGCCTCCAGA GCAGTGCCCCAGTGCACTGAGATGCCCCCACTCCCATGGCCATAGTGGTGGACTACAGGC AGCCTCTGTCCATCTCGCGCAAGGAGCTCTGTCTGCAGTCGCACGCCTGGCCTGTNAGGC CTCAAGCCCACCTTCTCCCTGATGTTGCAAGCTCCCCGGAGGAAGGGCTCCAAAACACAG CATCGGGAAAGAATCCTCTGCTATTTTCTGCATCCGGGGACAAGATCCATCCCCCTTTTT GCCTATTTCCCCCTAGGGCTTCATGGGGAGGCACCAGCATAAATCTTGGTTAGGCCACTT GCCCTCCCGGATAAAATGCCTACACA |
Restriction Sites | NotI-NotI |
ACCN | NM_004032 |
Insert Size | 1780 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004032.2, NP_004023.2 |
RefSeq Size | 1556 bp |
RefSeq ORF | 933 bp |
Locus ID | 8528 |
UniProt ID | Q99489 |
Domains | DAO |
Protein Pathways | Alanine, aspartate and glutamate metabolism |
Gene Summary | The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2019] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212769 | DDO (Myc-DDK-tagged)-Human D-aspartate oxidase (DDO), transcript variant 2 |
CNY 2,400.00 |
|
RC212769L3 | Lenti ORF clone of Human D-aspartate oxidase (DDO), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC212769L4 | Lenti ORF clone of Human D-aspartate oxidase (DDO), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG212769 | DDO (tGFP-tagged) - Human D-aspartate oxidase (DDO), transcript variant 2 |
CNY 4,000.00 |
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