D aspartate oxidase (DDO) (NM_004032) Human Untagged Clone

CAT#: SC117617

DDO (untagged)-Human D-aspartate oxidase (DDO), transcript variant 2



  "NM_004032" in other vectors (4)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-DDO Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DASOX; DDO-1; DDO-2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117617 sequence for NM_004032 edited (data generated by NextGen Sequencing)
ATGAGACCAGCCAGGCACTGGGAAACAAGGTTTGGTGCCAGAGATTTTGGTGNNTTCCAA
GACTGCTTTTTCAGAGACAGGCTCATGGACACAGCACGGATTGCAGTTGTCGGGGCAGGT
GTGGTGGGGCTCTCCACGGCTGTGTGCATCTCCAAACTGGTGCCCCGATGCTCCGTTACC
ATCATTTCAGACAAGTTTACTCCAGATACCACCAGTGATGTGGCAGCCGGAATGCTTATT
CCTCMCACTTATCCAGATACACCCATTCACACGCAGAAGCAGTGGTTCAGAGAAACCTTT
AATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTGGTGTTCATTTGGTA
TCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAGAAGACCTGTGGGAA
CTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAAGCAGACAGCTTGCA
GGAGACTCAAAGATTTTCCCTGTAAGGGGCCAAGTCCTCCAAGTTCAGGCTCCCTGGGTG
GAGCATTTTATCCGAGATGGCAGTGGGCTGACATATATTTATCCTGGTACATCCCATGTA
ACCCTAGGTGGAACTAGGCAAAAAGGGGACTGGAATCTGTCCCCGGATGCAGAAAATAGC
AGAGAGATTCTTTCCCGATGCTGTGTTCTGGAGCCCTCCCTCCACGGAGCCTGCAACATC
AGGGAGAAGGTGGGCTTGAGGCCCTACAGGCCAGGCGTGCGACTGCAGACAGAGCTCCTT
GCGCGAGATGGACAGAGGCTGCCTGTAGTCCACCACTATGGCCATGGGAGTGGGGGCATC
TCAGTGCACTGGGGCACTGCTCTGGAGGCCGCCAGGCTGGTGAGCGAGTGTGTCCATGCC
CTCAGGACCCCCATTCCCAAGTCAAACCTGTAG

Clone variation with respect to NM_004032.2
53 g=>n;54 c=>n;245 a=>m;686 c=>t
>OriGene 5' read for NM_004032 unedited
NGTTCAGGGAATTATGAAACCACGATTTCACTATAGGGCGGAACGCCAATTCGGCACGAG
GCTGTCTCTCTAGGCATGAGACCAGCCAGGCACTGCGAAACAGGTTTGGTGCCAGAGATT
TTGGTGGCTTCCAAGACTGCTTTTTCAGAGACAGGCTCATGGACACAGCACGGATTGCAG
TTGTCGGGGCAGGTGTGGTGGGGCTCTCCACGGCTGTTTTTTTCTCCAAACTGGTGCCCC
GATGCTCCGTTACCATCATTTCAGACAAGTTTACTCCAGATACCACCAGTGATGTGGCAG
CCGGAATGCTTATTCCTCACACTTATCCAGATACACCCATTCACACGCAGAAGCAGTGGT
TCAGAGAAACCTTTAATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTG
GTGTTCATTTGGTATCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAG
AAGACCTGTGGGAACTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAA
GCAGACAGCTTGCAGGAGACTCAAAGATTTTCCCTGTAAGGGGCCAAGTCCTCCAAGTTC
ACGCTCCCTGNGTGGAGCATTTTATCCGAGATGGCAGTGGGCTGACATATATTTATCCTG
GTACATCCCATGTAACCCCTAGTGGAACTAGGCAAAAAGGGACTGGAATCTGTCCCCCNA
TGCAGAAAATAGCAGAGAGATTCTCTCCCGATGCTGTGTTCTGGAGCCCTCCCTCCACGG
AGCCTGNCACATCAGGNAGAANGTGGGGCTTGAAGCCCTACAGCCCANGCGTGCGACTGC
AGACAGAGCTCCTTGCGCGAGATGGACAGAAGCTGCCCTGTATCCACCACTATGGCCATG
GAAGTGGGGCAATTCACTGCACTGGGGCN
>OriGene 3' read for NM_004032 unedited
TCCATGTTATTTTTTTTTTTAGTAATTATGTTGCTGACTTGGCTATAACCTAAGAGATCA
CAGCAGCTCTGCAATTGACAGTGATTTAAATGTTTTCCATACATTATCTCCTCCTGTGGA
TTTATAAGCCAAATGATATCAAAATCTCTATAGCTTCCAACATTCATATAAATCTGCATA
GGTCCTTGGACATCAGTTCTAAATAAATTTTACTCTATAAGAAGTATTTTTAACAAAAAA
TAGAGCTTTATGCCCTATGCCATTAATGCTGGACTTCCTAAGTAAGCCTACATGTTGCAT
CATTTCTTTTGTTGTTGGTGTTTTTTTTAGAGGTGGGAACTGGCACCTCTAAAAAATGTT
ACCCAGATTGCACTCAAACTCCTGGGCTCAACAATCCTCCTGCCTCAGCCTCTCAAGTAG
CTGGGACTATAGGCATGCCACCGTGCTCAGCTTACATGTTACACCACTTCTAATGTTGAA
AACAAAGAAATGTCTAATTAAACTTTCATGCAGTGGAAAAGTTATTTGAACCTGTTCTGT
GCTTTGATCAACAGTCTCTCAGTCTCTTTGCTGTCATTTTATGTCATCTACAGGTTTGAC
TTGGGAATGGGGGTCCTGAGGGCATGGACACACTCGCTCACCAGCCTGGCGGCCTCCAGA
GCAGTGCCCCAGTGCACTGAGATGCCCCCACTCCCATGGCCATAGTGGTGGACTACAGGC
AGCCTCTGTCCATCTCGCGCAAGGAGCTCTGTCTGCAGTCGCACGCCTGGCCTGTNAGGC
CTCAAGCCCACCTTCTCCCTGATGTTGCAAGCTCCCCGGAGGAAGGGCTCCAAAACACAG
CATCGGGAAAGAATCCTCTGCTATTTTCTGCATCCGGGGACAAGATCCATCCCCCTTTTT
GCCTATTTCCCCCTAGGGCTTCATGGGGAGGCACCAGCATAAATCTTGGTTAGGCCACTT
GCCCTCCCGGATAAAATGCCTACACA
Restriction Sites NotI-NotI     
ACCN NM_004032
Insert Size 1780 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004032.2, NP_004023.2
RefSeq Size 1556 bp
RefSeq ORF 933 bp
Locus ID 8528
UniProt ID Q99489
Domains DAO
Protein Pathways Alanine, aspartate and glutamate metabolism
Gene Summary The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2019]
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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