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BYSL (NM_004053) Human Untagged Clone
CAT＃: SC117600
BYSL (untagged)-Human bystin-like (BYSL)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BYSTIN; Enp1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117600 sequence for NM_004053 edited (data generated by NextGen Sequencing)
ATGCCCAAATTCAAGGCGGCCCGTGGGGTGGGGGGTCAGGAAAAACATGCGCCCCTGGCC GATCAGATCCTGGCTGGGAATGCGGTGCGGGCGGGGGTCCGGGAGAAGCGGCGGGGTCGC GGGACAGGAGAAGCGGAGGAAGAGTATGTGGGGCCCCGGCTGAGCCGACGGATTTTGCAG CAAGCACGGCAGCAACAGGAGGAACTCGAGGCCGAGCATGGGACTGGGGACAAGCCCGCG GCGCCGCGGGAACGCACCACGCGGCTGGGTCCAAGAATGCCTCAGGATGGATCAGATGAC GAGGACAAGGAGTGGCCCACCCTGGAGAAGGCTGCCACAATGACAGCAGCGGGCCATCAT GCAGAGGTGGTTGTGGACCCTGAGGATGAGCGTGCCATAGAGATGTTCATGAACAAGAAC CCTCCTGCCAGGCGCACCCTGGCTGACATCATCATGGAGAAGCTGACTGAGAAGCAGACA GAGGTTGAGACAGTCATGTCAGAGGTGTCGGGCTTCCCTATGCCCCAGCTGGACCCCCGG GTCCTAGAAGTGTACAGGGGGGTCCGGGAGGTATTATCTAAGTACCGCAGTGGAAAACTG CCCAAGGCATTTAAGATCATCCCTGCACTCTCCAACTGGGAGCAAATCCTCTACGTCACA GAGCCGGAGGCCTGGACTGCAGCTGCCATGTACCAGGCCACCAGGATTTTTGCCTCTAAC CTGAAGGAACGCATGGCCCAGCGCTTCTACAACCTTGTCCTGCTCCCTCGAGTACGAGAT GACGTTGCTGAATACAAACGACTCAACTTCCATCTCTACATGGCTCTCAAGAAGGCCCTT TTCAAACCTGGAGCCTGGTTCAAAGGGATCCTGATTCCACTGTGCGAGTCTGGCACTTGT ACCCTCCGGGAAGCCATCATTGTGGGTAGCATCATCACCAAGTGCTCCATCCCTGTGTTG CACTCCAGTGCGGCCATGCTGAAAATTGCTGAGATGGAATACAGCGGTGCCAACAGCATC TTCCTGCGACTGCTGCTGGATAAGAAGTATGCACTGCCTTACCGGGTGCTGGATGCCCTA GTCTTCCACTTCCTGGGGTTCCGGACAGAGAAGCGTGAACTGCCTGTGCTGTGGCACCAG TGCCTCCTGACTTTGGTCCAGCGCTACAAGGCCGACTTGGCCACAGACCAGAAAGAGGCC CTCTTAGAACTGCTCCGGCTGCAGCCCCATCCACAGCTATCGCCCGAAATCAGGCGTGAG CTTCAGAGTGCAGTCTCCCGCGATGTGGAAGATGTTCCCATCACCGTGGAGTGA Clone variation with respect to NM_004053.3 307 g=>a;1276 c=>t >OriGene 5' read for NM_004053 unedited
TTTTGTATACGACTACTATAGGCGGCCGCGAATTCGGCACGAGGCACGTGCGATCCTTCC CGGCAACTTTTTCGAGAAAAATGCCCAAATTCAAGGCGGCCCGTGGGGTGGGGGGTCAGG AAAAACATGCGCCCCTGGCCGATCAGATCCTGGCTGGGAATGCGGTGCGGGCGGGGGTCC GGGAGAAGCGGCGGGGTCGCGGGACAGGAGAAGCGGAGGAAGAGTATGTGGGGCCCCGGC TGAGCCGACGGATTTTGCAGCAAGCACGGCAGCAACAGGAGGAACTCGAGGCCGAGCATG GGACTGGGGACAAGCCCGCGGCGCCGCGGGAACGCACCACGCGGCTGGGTCCAAGAATGC CTCAGGATGGATCAGATGACGAGGACAAGGAGTGGCCCACCCTGGAGAAGGCTGCCACAA TGACAGCAGCGGGCCATCATGCAGAGGTGGTTGTGGACCCTGAGGATGAGCGTGCCATAG AGATGTTCATGAACAAGAACCCTCCTGCCAGGCGCACCCTGGCTGACATCATCATGGAGA AGCTGACTGAGAAGCAGACAGAGGTTGAGACAGTCATGTCAGAGGTGTCGGGCTTCCCTA TGCCCCAGCTGGACCCCCGGGTCCTAGAAGTGTACAGGGGGGTCCGGGAGGTATTATCTA AGTACCGCAGTGGAAAACTGCCCAAGGCATTTAAGATCATCCCTGCACTCTNNCACTGGG AGCANATCCTCTACGTCACAGAGCCGGGAGCCTGGACTGCAGCTGNCATGTACCANGCCA CCCAGNATTTTTGCCTCTAACCCTGAGGAACGCATGGNCCAGCGCTTCTACAACCTTGTN CTGCTCCCTCGAGTACGAGATGACGTTGCTGAATACCAACGACTCAACTTNCATCTCTAC ATGGCTCTCAAGAAGGCCCTTTTCAACCTGGAGCCCTGGTCANAGGGATCCTGATNCACT GTGCGAGTCTGGCACTTGTACCTNCGGNNAGCATCATGTGGNTAN >OriGene 3' read for NM_004053 unedited
CAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCAATAAATATCTGTCTTTGCCTTTATC CACAGTGACCTGACACAATAGCCATAGAAAAGTACTCACACCAAGCCCCAGCTGGGGACT GGGAATGCTAGCATATGGTATCTCAAGTTGGCTCTCAGAACTAAACAGGGATAAGGGCCT AGAATGGAAGAGGGAACCAGCCAGACCCTCAGTCCTTCCTGTCCTGGACTGGGAGCCACA GATGTCCCTGTGATCTGTCACTGCCCTGATCTGGGTCTTCAGCCATTAAAGCTCAGTGTC ATCTTCAGTCACCAACGGGGGTCTTGGTGTCCTTCCAAACCCCTTTGGCCAGGACAGCTG ACTGTTTTCCTCACTCCACGGTGATGGGAACATCTTCCACATCGCGGGAGACTGCACTCT GAAGCTCACGCCTGATTTCGGGCGATAGCTGTGGATGGGGCTGCAGCCGGAGCAGTTCTA AGAGGGCCTCTTTCTGGTCTGTGGCCAAGTCGGCCTTGTAGCGCTGGACCAAAGTCAGGA GGCACTGGTGCCACAGCACAGGCAGTTCACGCTTCTCTGTCCGGAACCCCAGGAAGTGGA AGACTAGGGCATCCAGCACCCGGTAAGGCAGTGCATACTTCTTTATCCAGCAGCAGTCGC AGGAAGATGCTTGTTGGCACCGCTGTATTTCCATCTCAGCAATTTTCAGCATGGCCGCCC TGAAGTGCACCACCAGGAATGGAGCCCTCGGTGATGATGCTACCCCCATGATGGCTTCCC GGAGGGTACAGGGCCAGACTGCACAGTGGAATAAGATCCCTTTGACCCAGCTTCAGGTTG AAAAGGCCTTCTGAAACCCTGTAAAAATGAATTGGATCTTTGTATCACAACGTATCTCTA CTTAAGGACAGAAAGGTTGAAACCGTCTGGCATGCTCTTTAGTAGAGCAAAACCGGGGCT CGACATGAATGAATCAACCTCGTTTGGACAAAGATCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004053 |
| Insert Size | 1790 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004053.3, NP_004044.3 |
| RefSeq Size | 2005 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 705 |
| UniProt ID | Q13895 |
| Protein Families | Stem cell - Pluripotency |
| Gene Summary | Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208675 | BYSL (Myc-DDK-tagged)-Human bystin-like (BYSL) |
CNY 3,656.00 |
|
| RC208675L1 | Lenti ORF clone of Human bystin-like (BYSL), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208675L2 | Lenti ORF clone of Human bystin-like (BYSL), mGFP tagged |
CNY 5,890.00 |
|
| RC208675L3 | Lenti ORF clone of Human bystin-like (BYSL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208675L4 | Lenti ORF clone of Human bystin-like (BYSL), mGFP tagged |
CNY 6,056.00 |
|
| RG208675 | BYSL (tGFP-tagged) - Human bystin-like (BYSL) |
CNY 5,256.00 |
