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EAAT1 (SLC1A3) (NM_004172) Human Untagged Clone
CAT＃: SC117514
SLC1A3 (untagged)-Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1
CNY 4,040.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EA6; EAAT1; GLAST; GLAST1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117514 sequence for NM_004172 edited (data generated by NextGen Sequencing)
ATGACTAAAAGCAATGGAGAAGAGCCCAAGATGGGGGGCAGGATGGAGAGATTCCAGCAG GGAGTCCGTAAACGCACACTTTTGGCCAAGAAGAAAGTGCAGAACATTACAAAGGAGGAT GTTAAAAGTTACCTGTTTCGGAATGCTTTTGTGCTGCTCACAGTCACCGCTGTCATTGTG GGTACAATCCTTGGATTTACCCTCCGACCATACAGAATGAGCTACCGGGAAGTCAAGTAC TTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAGATGCTGGTCTTACCACTTATC ATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGTAAGGCATCAGGGAAGATGGGA ATGCGAGCTGTAGTCTATTATATGACTACCACCATCATTGCTGTGGTGATTGGCATAATC ATTGTCATCATCATCCATCCTGGGAAGGGCACAAAGGAAAACATGCACAGAGAAGGCAAA ATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTGATCAGGAACATGTTCCCTCCA AATCTGGTAGAAGCCTGCTTTAAACAGTTTAAAACCAACTATGAGAAGAGAAGCTTTAAA GTGCCCATCCAGGCCAACGAAACGCTTGTGGGTGCTGTGATAAACAATGTGTCTGAGGCC ATGGAGACTCTTACCCGAATCACAGAGGAGCTGGTCCCAGTTCCAGGATCTGTGAATGGA GTCAATGCCCTGGGTCTAGTTGTCTTCTCCATGTGCTTCGGTTTTGTGATTGGAAACATG AAGGAACAGGGGCAGGCCCTGAGAGAGTTCTTTGATTCTCTTAACGAAGCCATCATGAGA CTGGTAGCAGTAATAATGTGGTATGCCCCCGTGGGTATTCTCTTCCTGATTGCTGGGAAG ATTGTGGAGATGGAAGACATGGGTGTGATTGGGGGGCAGCTTGCCATGTACACCGTGACT GTCATTGTTGGCTTACTCATTCACGCAGTCATCGTCTTGTCACTCCTCTACTTCTTGGTA ACACGGAAAAACCCTTGGGTTTTTATTGGAGGGTTGCTGCAAGCACTCATCACCGCTCTG GGGACCTCTTCAAGTTCTGCCACCCTACCCATCACCTTCAAGTGCCTGGAAGAGAACAAT GGCGTGGACAAGCGCGTCACCAGATTCGTGCTCCCCGTAGGAGCCACCATTAACATGGAT GGGACTGCCCTCTATGAGGCTTTGGCTGCCATTTTCATTGCTCAAGTTAACAACTTTGAA CTGAACTTCGGACAAATTATTACAATCAGCATCACAGCCACAGCTGCCAGTATTGGGGCA GCTGGAATTCCTCAGGCGGGCCTGGTCACTATGGTCATTGTGCTGACATCTGTCGGCCTG CCCACTGACGACATCACGCTCATCATCGCGGTGGACTGGTTCCTGGATCGCCTCCGGACC ACCACCAACGTACTGGGAGACTCCCTGGGAGCTGGGATTGTGGAGCACTTGTCACGACAT GAACTGAAGAACAGAGATGTTGAAATGGGTAACTCAGTGATTGAAGAGAATGAAATGAAG AAACCATATCAACTGATTGCACAGGACAATGAAACTGAGAAACCCATCGACAGTGAAACC AAGATGTAG Clone variation with respect to NM_004172.4 1000 c=>t >OriGene 5' read for NM_004172 unedited
GGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCAGGGC TAGCCTGCCTGCTTACGCGCGCTGCGGATTGTTGCTCCGTTGTACCTGCTGGGGAATTCA CCTCGTTACTGCTTGATATCTTCCACCCCTTACAAAATCAGAAAAGTTGTGTTTTCTAAT ACCAAAGAGGAGGTTTGGCTTTCTGTGGGTGATTCCCAGACACTGAAGTGCAAAGAAGAG ACCCTCCTAGAAAAGTAAAATATGACTAAAAGCAATGGAGAAGAGCCCAAGATGGGGGGC AGGATGGAGAGATTCCAGCAGGGAGTCCGTAAACGCACACTTTTGGCCAAGAAGAAAGTG CAGAACATTACAAAGGAGGATGTTAAAAGTTACCTGTTTCGGAATGCTTTTGTGCTGCTC ACAGTCACCGCTGTCATTGTGGGTACAATCCTTGGATTTACCCTCCGACCATACAGAATG AGCTACCGGGAAGTCAAGTACTTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAG ATGCTGGTCTTACCACTTATCATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGT AAGGCATCAGGGAAGATGGGAATGCGAGCTGTAGTCTATTATATGACTACCACCATCATT GCTGTGGTGATTGGCATAATCATTGTCATCATCATCCATCCTGNGAAGGGCACAAAGGAA AACATGCACAGAGAAGGCAAAATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTG ATCAGGAACATGTTCCCTTNCANATCTGTAGAAGCCTGCTTTANACAGTTTAAAACCAAC TATGAGAAGAGAAGCTNNTAAGTGCCCATCCAGACCAACGAAACGCTTGTGGGTGCTGTG ATAAACAATGTGTCTGA >OriGene 3' read for NM_004172 unedited
GCAACTTTCCAGGGCCAGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT TTTTAATGTAAAACTATTTTTTTCTTTATTGAAAATACATCTACAAAGTAATGCTTCCCA GTCCACACTGAGTGCATGTGTATGCAAAGCCTGTGATGGCTTGGCGAGGGGATGGGCTCC CCACAGGACCTTTCCATAAGAAATGGTTAGTATATTAAAAAACACATATTGTGTAACAGA ATTAAGTTGATATGATTAAGTCTTTGCTCTACTAGTGTCTTAAGAGGACAGCCACGTACT TTAACATTTGTAGTGTATTTCCGAAATAGAGCCTCGACATTAAATGGTTACTGCACCACA GCCTAATGTTAACTTTTATGCACAGAATGCTTGGGGTCTATATAAAAATATCTCTAATTG TATATCAGAGGATCTATGTCTAGATTTTTCAGTTTGTTAGCATAATTAAGTCCATTCTTC ACCATTTTCAGCCAGAAACTCAAACACAGCTGCTACTGCCTGACAGCTCCCACTCAGCGT CTTTGACTGGATATTCCTTANGGGGTAAAAATTAATCTCTAAACCTGGNGAGCTGCCTTG CCTGAAACTAATGCCCNCCTGCTGTTTTTTTCTCATAAACCTCATTGACAGNNGNCTTCA TACTGGGATTCATCTTCTACCATTTTCTCTTNATGGNNACACAAACANACANAAAGCAAC CCCACTTCAGCNTGAAAGTTTTTGAATGATTTGGAATAAGTGGGGATTCCAAACAAATAA AATATTTTGGTTTCCCTCATTCTAAGGGACCTTTGGATTAAAGAAAAACACTTTCNATAA NGAATACCCACTCACTCCTGGGACTTCTTTAAAGAACTCATCTTTTGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004172 |
| Insert Size | 1629 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004172.3, NP_004163.2 |
| RefSeq Size | 3983 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 6507 |
| UniProt ID | P43003 |
| Domains | SDF |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014] Transcript Variant: This variant (GLAST) represents the longest transcript and encodes the longest protein (isoform 1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Arundic acid attenuates retinal ganglion cell death by increasing glutamate/aspartate transporter expression in a model of normal tension glaucoma
,Yanagisawa, M;Aida, T;Takeda, T;Namekata, K;Harada, T;Shinagawa, R;Tanaka, K;,
Cell Death Dis
,PubMed ID 25789968
[SLC1A3]
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Other Versions
| SKU | Description | Size | Price |
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| RC207205 | SLC1A3 (Myc-DDK-tagged)-Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1 |
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| RC207205L3 | Lenti-ORF clone of SLC1A3 (Myc-DDK-tagged)-Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1 |
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| RC207205L4 | Lenti-ORF clone of SLC1A3 (mGFP-tagged)-Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1 |
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| RG207205 | SLC1A3 (tGFP-tagged) - Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1 |
CNY 8,056.00 |
