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TFIIA2 (GTF2A2) (NM_004492) Human Untagged Clone
CAT＃: SC117338
GTF2A2 (untagged)-Human general transcription factor IIA, 2, 12kDa (GTF2A2)
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HsT18745; T18745; TF2A2; TFIIA; TFIIA-12; TFIIA-gamma; TFIIAS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117338 sequence for NM_004492 edited (data generated by NextGen Sequencing)
ATGGCATATCAGTTATACAGAAATACTACTTTGGGAAACAGTCTTCAGGAGAGCCTAGAT GAGCTCATACAGTCTCAACAGATCACCCCCCAACTTGCCCTTCAAGTTCTACTTCAGTTT GATAAGGCTATAAATGCAGCACTGGCTCAGAGGGTCAGGAACAGAGTCAATTTCAGGGGC TCTCTAAATACGTACAGATTCTGCGATAATGTGTGGACTTTTGTACTGAATGATGTTGAA TTCAGAGAGGTGACAGAACTTATTAAAGTGGATAAAGTGAAAATTGTAGCCTGTGATGGT AAAAATACTGGCTCCAATACTACAGAATGA Clone variation with respect to NM_004492.2 >OriGene 5' read for NM_004492 unedited
TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTCTGCGGTTTCTGCG GCGGCTAGAGAGGTGGTCGGAGAAGTAGGAACCTCCTGCCGGGCTCGTGGCGGCTTCTGT CCGCTCCGCGGCGGGAAGCGCCTTCCCCACAGGACATCAATGCAAGCTTGAATAAGAAAA ACAAATTCTTCCTCCTAAGCCATGGCATATCAGTTATACAGAAATACTACTTTGGGAAAC AGTCTTCAGGAGAGCCTAGATGAGCTCATACAGTCTCAACAGATCACCCCCCAACTTGCC CTTCAAGTTCTACTTCAGTTTGATAAGGCTATAAATGCAGCACTGGCTCAGAGGGTCAGG AACAGAGTCAATTTCAGGGGCTCTCTAAATACGTACAGATTCTGCGATAATGTGTGGACT TTTGTACTGAATGATGTTGAATTCAGAGAGGTGACAGAACTTATTAAAGTGGATAAAGTG AAAATTGTAGCCTGTGATGGTAAAAATACTGGCTCCAATACTACAGAATGAATAGAAAAA ATATGACTTTTTTACACCATCTTCTGTTATTCATTGCTTTTGAAGAGAAGCATAGAAGAG ACTTTTTATTTATTCTAGAATTGCAGAAATGACTACACTGTGCTATACCAGAGAATTCCA GTAGAAAGAAACTTGTAACTCTGTAGCCTCTTACATCACCTTTATTATACAGCATGAAAA AACCATAACTTCTTTTTAGGGACAAAAGNTGNTGCCCTTCTAAGAACCTTNCTTATAAAC TCATNTAAAACTCTGAGTATAACTGGTGAAGCTATNAGGTAGCATATGAAGAATAGATAT GCATTTTCCATTTCTTGCAGGACATGATGCCTTTCAAATGCTATTCATAAGNCATGTTTG GC >OriGene 3' read for NM_004492 unedited
AGGTCCTATCTCTGNACCGCGGCCCGCTTTCTAGNANCGAGTTTTTTCTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACACCCGACAAAACATGACTTTATTGA AATAAGCTTTCTGAAAGGGCATCATGTTCCTTGCAAAGGAAAGGGGAAAAGGCATTATCT AATATCTTCATATTGCTACCTTAATAGCTTCACCAGTTATTACTCAAAGTTTTAAAAGGA GTTTATTAAAAAAGGTTTTTAGGAAGGCAACAACTTTTGTCCTTAAAAAAAAGTTATGGT TTTTCATGCTGTATAAAAAAGGGGATGTAAGAGGCTACAGAGTTACAAGTTTCTTTTTAC TGGAATTCTCTGGTATAGCACAGGGTACTCATTTCTGCAATTCTAAAATAAATAAAAAGT CTCTTCTATGCTTCTCTTCAAAAGCAATGAATAACAGAAGATGGGGTAAAAAAGGCATAT TTTTTCTATTCATTCCGAACAATCGGACCCAGTATTTTTACCATCACAGGCTACAATTTT CACTTTATCCACTTTAATAAGTTCTGCCCCCCTTCTGAATTCACCATTCTTCAGTACAAA AGTCCACACATTATTCGCAAAATCTGTACCTATTTAAAGAGCCCCTGAAATTGACTCTGT TCCTGACCCTCTGAGCCCAGGCTGCATTTATAGCCTTATCAAACTGAAGTAAAACTTTGA GGCCACGTCGGGGGGCGATCCGTTGACACTGTATGAACCTATTTAGGCTTCCCTGAAGAT TGTTTCCCAAAAAACATTTTCGTATAACTGATATCCCGGGTCTTAGGAGGAAAAATTTGC TTTCTTATTAACTTCGGCTTGAGTCCCGCGGGGAAGCCTCTTCCCCCGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004492 |
| Insert Size | 920 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004492.1, NP_004483.1 |
| RefSeq Size | 736 bp |
| RefSeq ORF | 330 bp |
| Locus ID | 2958 |
| UniProt ID | P52657 |
| Domains | TFIIA_gamma |
| Protein Families | Transcription Factors |
| Protein Pathways | Basal transcription factors |
| Gene Summary | Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201117 | GTF2A2 (Myc-DDK-tagged)-Human general transcription factor IIA, 2, 12kDa (GTF2A2) |
CNY 1,200.00 |
|
| RC201117L1 | Lenti ORF clone of Human general transcription factor IIA, 2, 12kDa (GTF2A2), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC201117L2 | Lenti ORF clone of Human general transcription factor IIA, 2, 12kDa (GTF2A2), mGFP tagged |
CNY 5,890.00 |
|
| RC201117L3 | Lenti ORF clone of Human general transcription factor IIA, 2, 12kDa (GTF2A2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201117L4 | Lenti ORF clone of Human general transcription factor IIA, 2, 12kDa (GTF2A2), mGFP tagged |
CNY 5,890.00 |
|
| RG201117 | GTF2A2 (tGFP-tagged) - Human general transcription factor IIA, 2, 12kDa (GTF2A2) |
CNY 4,370.00 |
