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Topoisomerase III alpha (TOP3A) (NM_004618) Human Untagged Clone
CAT＃: SC117267
TOP3A (untagged)-Human topoisomerase (DNA) III alpha (TOP3A)
CN¥ 10,464.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGRISCE2; PEOB5; TOP3; ZGRF7 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004618, the custom clone sequence may differ by one or more nucleotides
ATGATCTTTCCTGTCGCCCGCTACGCGCTCCGGTGGCTGCGACGGCCCGAAGACCGTGCCTTTTCCCGCG CCGCCATGGAGATGGCCCTCCGAGGCGTGCGGAAAGTCCTCTGTGTGGCCGAAAAAAACGACGCGGCCAA GGGGATCGCCGACCTGCTGTCAAACGGTCGCATGAGGCGGAGAGAAGGACTTTCAAAATTCAACAAGATC TATGAATTTGATTATCATCTGTATGGCCAGAATGTTACCATGGTAATGACTTCAGTTTCTGGACATTTAC TGGCTCATGATTTCCAGATGCAGTTTCGAAAATGGCAGAGCTGCAACCCTCTTGTCCTCTTTGAAGCAGA AATTGAAAAGTACTGCCCAGAGAATTTTGTAGACATCAAGAAAACTTTGGAACGAGAGACTCGCCAGTGC CAGGCTCTGGTGATCTGGACTGACTGTGATAGAGAAGGCGAAAACATCGGGTTTGAGATTATCCACGTGT GTAAGGCTGTAAAGCCCAATCTGCAGGTGTTGCGAGCCCGATTCTCTGAGATCACACCCCATGCCGTCAG GACAGCTTGTGAAAACCTGACCGAGCCTGATCAGAGGGTGAGCGATGCTGTGGATGTGAGGCAGGAGCTG GACCTGAGGATTGGAGCTGCCTTTACTAGGTTCCAGACCCTGCGGCTTCAGAGGATTTTTCCTGAGGTGC TGGCAGAGCAGCTCATCAGTTACGGCAGCTGCCAGTTCCCCACACTGGGCTTTGTGGTGGAGCGGTTCAA AGCCATTCAGGCTTTTGTACCAGAAATCTTCCACAGAATTAAAGTAACTCATGACCACAAAGATGGTATC GTAGAATTCAACTGGAAAAGGCATCGACTCTTTAACCACACGGCTTGCCTAGTTCTCTATCAGTTGTGTG TGGAGGATCCCATGGCAACTGTGGTAGAGGTCAGATCTAAGCCCAAGAGCAAGTGGCGGCCTCAAGCCTT GGACACTGTGGAGCTTGAGAAGCTGGCTTCTCGAAAGTTGAGAATAAATGCTAAAGAAACCATGAGGATT GCTGAGAAGCTCTACACTCAAGGGTACATCAGCTATCCCCGAACAGAAACAAACATTTTTCCCAGAGACT TAAACCTGACGGTGTTGGTGGAACAGCAGACCCCCGATCCACGCTGGGGGGCCTTTGCCCAGAGCATTCT AGAGCGGGGTGGTCCCACCCCACGCAATGGGAACAAGTCTGACCAAGCTCACCCTCCCATTCACCCCACC AAATACACCAACAACTTACAGGGAGATGAACAGCGACTGTACGAGTTTATTGTTCGCCATTTCCTGGCTT GCTGCTCCCAGGATGCTCAGGGGCAGGAGACCACAGTGGAGATCGACATCGCTCAGGAACGCTTTGTGGC CCATGGCCTCATGATTCTGGCCCGAAACTATCTGGATGTGTATCCATATGATCACTGGAGTGACAAGATC CTCCCTGTCTATGAGCAAGGATCCCACTTTCAGCCCAGCACCGTGGAGATGGTGGACGGGGAGACCAGCC CACCCAAGCTGCTCACCGAGGCCGACCTCATTGCCCTCATGGAGAAGCATGGCATTGGTACGGATGCCAC TCATGCGGAGCACATCGAGACCATCAAAGCCCGGATGTACGTGGGCCTCACCCCAGACAAGCGGTTCCTC CCTGGGCACCTGGGCATGGGACTTGTGGAAGGTTATGATTCCATGGGCTATGAAATGTCTAAGCCTGACC TCCGGGCTGAACTGGAAGCTGATCTGAAGCTGATCTGTGATGGCAAAAAGGACAAATTTGTGGTTCTAAG GCAGCAAGTGCAGAAATACAAGCAGGTTTTCATTGAAGCGGTGGCTAAAGCAAAGAAATTGGACGAGGCC TTGGCCCAGTACTTTGGGAATGGGACAGAGTTGGCCCAGCAAGAAGATATCTACCCAGCCATGCCAGAGC CCATCAGGAAGTGCCCACAGTGCAACAAGGACATGGTCCTTAAGACCAAGAAGAATGGCGGGTTCTACCT CAGCTGCATGGGTTTCCCAGAGTGTCGCTCAGCTGTGTGGCTTCCTGACTCGGTGCTGGAGGCCAGCAGG GACAGCAGTGTGTGTCCAGTTTGTCAGCCACACCCTGTGTACAGGTTAAAGTTAAAGTTTAAGCGCGGTA GCCTTCCCCCGACCATGCCTCTGGAGTTTGTTTGCTGCATCGGCGGATGCGACGACACCCTGAGGGAGAT CCTGGACCTGAGATTTTCAGGGGGCCCCCCCAGGGCTAGCCAGCCCTCTGGCCGCCTGCAGGCTAACCAG TCCCTGAACAGGATGGACAACAGCCAGCACCCCCAGCCTGCTGACAGCAGACAGACTGGGTCCTCAAAGG CTCTGGCCCAGACCCTCCCACCACCCACGGCTGCTGGTGAAAGCAATTCTGTGACCTGCAACTGTGGCCA GGAGGCTGTGCTGCTCACTGTCCGTAAGGAGGGCCCCAACCGGGGCCGGCAGTTCTTTAAGTGCAACGGA GGTAGCTGCAACTTCTTCCTGTGGGCAGACAGCCCCAATCCGGGAGCAGGAGGGCCTCCTGCCTTGGCAT ATAGACCCCTGGGCGCCTCCCTGGGATGCCCACCAGGCCCAGGGATCCACCTAGGTGGGTTTGGCAACCC TGGTGATGGCAGTGGTAGTGGCACATCCTGCCTTTGCAGCCAGCCCTCCGTCACACGGACTGTGCAGAAG GATGGACCCAACAAGGGGCGCCAGTTCCACACATGTGCCAAGCCGAGAGAGCAGCAGTGTGGCTTTTTCC AGTGGGTCGATGAGAACACCGCTCCAGGGACTTCTGGAGCCCCGTCCTGGACAGGAGACAGAGGAAGAAC CCTGGAGTCGGAAGCCAGAAGCAAAAGGCCCCGGGCCAGTTCCTCAGACATGGGGTCCACAGCAAAGAAA CCCCGGAAATGCAGCCTTTGCCACCAGCCTGGACACACCCGTCCCTTTTGTCCTCAGAACAGATGA >OriGene 5' read for NM_004618 unedited
GTTCAAATTTTGTAATACGACTCACTATAGGGACTGGCCGCGATTCGGCACGAGGAGTGA CTGAAGCTCCAAAGGCCAGCAGGCTGGTGGGGACGTGACCGAAGCGAGGCTCTGGTTCCC TTTCGGTGGGCGCCATTTGAGCCTCATCTCTGGCTTCCCCAGGATGCGCCGGCAGCCGGG GAGCGGCTCCGGGCGCGAGGTCTGAGGATGATCTTTCCTGTCGCCCGCTACGCGCTCCGG TGGCTGCGACGGCCCGAAGACCGTGCCTTTTCCCGCGCCGCCATGGAGATGGCCCTCCGA GGCGTGCGGAAAGTCCTCTGTGTGGCCGAAAAAAACGACGCGGCCAAGGGGATCGCCGAC CTGCTGTCAAACGGTCGCATGAGGCGGAGAGAAGGACTTTCAAAATTCAACAAGATCTAT GAATTTGATTATCATCTGTATGGCCAGAATGTTACCATGGTAATGACTTCAGTTTCTGGA CATTTACTGGCTCATGATTTCCAGATGCAGTTTCGAAAATGGCAGAGCTGCAACCCTCTT GTCCTCTTTGAAGCAGAAATTGAAAAGTACTGCCCAGAGAATTTTGTAGACATCAAGAAA ACTTTGGAACGAGAGACTCGCCAGTGCCAGGCTCTGGTGATCTGGACTGACTGTGATAGA GAAGGCGAAAACATCGGGTTTGAGATTATCCACGTGTGTAAGGCTGTAAAGCCCAATCTG CANGTGTTGCGAGCCCGATTCTCATAGATCACACCCCATGCCGTCAGGACAGCTTGTGAA AACCTGACCGAGCCTGATCANAGGGTGAGCGATGCTGTGGATGTGAGGCAGGAGCTGGAC CCTGAGATTGGAGCTGCCTTTACTANGTTCCAGACCCCTGCGCTTCAGNANATTNTTCCC TGAGTGCTGGCAGACAGCTCATCAGTACGGAGCTGC >OriGene 3' read for NM_004618 unedited
TACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAAATTTTTAAATTTT TTTTGAGACTGAGTCTCGCTCTGTCGTCCAGTGGCGCAATCTCAGCTAACTGCAACCTCC GCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCCGGGTCCACAGGCG CACACCGCCACACCTGGCTAATTTTTTGTATTTCAGCAGAGACAGGGTTTCACCATGTTG CCCTGGCTGGTCTCGAACTCCTGAGCTCAGGCAATCCGCCCCCCACAGCCTCCCAAAGTG CTGGGATTACAGGCATGATCCACTGCACCCGGCCTGGGTTCTTTATTAAAGCAATGAATT CTGGATGTCCTGGCTTAACTAGATTAAGAGGGAATGAAGCTTCTTCTGAAGGATCAGTGT TACCACAGCCATTTCCTTGCATTACACCGTCCTTCTCAGGACGTCTATGATAAGCCCTGG GCAGCCTGGACAGGGACTACCCCATGAGTGCTGGTGGCTCACTTGGGGCTTTCTGCAGGT GAAGGAAGCAGCCCTGGGAGCTTCTTCCTTTGGCACACAGTGGGCTTCAGCTGGGACTGC ACCAATCCTCTGCTAACAGCAACCATCCTTGGGGGGTCCGAGGGAGCAGCTGCGTGACTT TTCAGCAGTGGCTATGGTCACTCCTGAGGCCCTGGAAGAGGTCACTGTCCAGCAGAGCTG GCCTGCTCCAGATGATCTGGACCTCGGGCCCTTACACAAGAGGCCCGACTCCAAGGCCAC ACTGTCCCTTAGTTTCCAGGACACTATAGCCACTTGGCCTGGGTAACTATTTTTAACACA AAGGGACGGTCTGGAAATTGGGTTCCTACCCTACTGGACCTTTGTCTGAGCACAAGGGAG GGGGGGCCAGCTGTGCAAAAGGTATTTCCCGGGTTTTTGTTGGGACCAATTTGTGAATTG CCGGGCCTCTTTTCTGTTCCGAATCAGGTTTTCCCCGTCCTTGAAAAAGGGCCAAATCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004618 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004618.3, NP_004609.1 |
| RefSeq Size | 4116 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 7156 |
| UniProt ID | Q13472 |
| Domains | Topoisom_bac, zf-CCHC, TOP1Bc, TOP1Ac, TOPRIM, Toprim |
| Protein Families | Druggable Genome |
| Protein Pathways | Homologous recombination |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208236 | TOP3A (Myc-DDK-tagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
CN¥ 10,456.00 |
|
| RC208236L3 | Lenti-ORF clone of TOP3A (Myc-DDK-tagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
CN¥ 11,880.00 |
|
| RC208236L4 | Lenti-ORF clone of TOP3A (mGFP-tagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
CN¥ 12,856.00 |
|
| RG208236 | TOP3A (tGFP-tagged) - Human topoisomerase (DNA) III alpha (TOP3A) |
CN¥ 12,056.00 |
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