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Apg12 (ATG12) (NM_004707) Human Untagged Clone
CAT＃: SC117215
ATG12 (untagged)-Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APG12; APG12L; FBR93; HAPG12 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004707 edited
GAATTCGGCACGAGGGTAGTCGCGGAGAAACACATGTTGCGTTACTAACGTTCAGAGGTC TGCGACAGCTTCGATTTGAATGACTAGCCGGGAACACCAAGTTTCACTGTGTAATTGCGT CCCCCTACTCCGGCGCCTCCTTTGCGACGCTCCCTGGAGAAAAGCACGCCCACTGCACGC GCTCAGTCGCTACTTCCGCTCTCGAGTGTCTCCAAGCAAGATGGCGGAGGAGCCGCAGTC TGTGTTGCAGCTTCCTACTTCAATTGCTGCTGGAGGGGAAGGACTTACGGATGTCTCCCC AGAAACAACCACCCCGGAGCCCCCGTCTTCCGCTGCAGTTTCCCCGGGAACAGAGGAACC TGCTGGCGACACCAAGAAAAAAATTGACATTTTGCTAAAGGCTGTGGGAGACACTCCTAT TATGAAAACAAAGAAGTGGGCAGTAGAGCGAACACGAACCATCCAAGGACTCATTGACTT CATCAAAAAGTTTCTTAAACTTGTGGCCTCAGAACAGTTGTTTATTTATGTGAATCAGTC CTTTGCTCCTTCCCCAGACCAAGAAGTTGGAACTCTCTATGAGTGTTTTGGCAGTGATGG TAAACTGGTTTTACATTACTGCAAGTCTCAGGCGTGGGGATGAACCACAAAGAAAATCAA CTTGCTACTACATGAAATGGATTTTCACGGAAGAGACAGCTCTGAAAAGTTTTGATGCTT GTGGCAAGAGACTTAACAGATGTGATCTATTTAGTATGTGTCTACTCTGTGTTTATGCAT AAGAAAACATCCATAGCATGAATGGACTCAGAAAAATGTGATTTGTATTAATGCACCAGT CATCATAAAAGATGGTCATGATAGTACACCCATTGCTCCTACTTGTTACTATTATTGCTG CAGATCTGCCTCCAAGGTTGAAAAGGAGACTAAGACTGTATAAACATCTTCATTGTCAGT TCTCAAAATGACTGAAATTGTTTTCXXXXXXXXXXXXXXCTCGAC >OriGene 5' read for NM_004707 unedited
NAGGTCAGTATTTGTATACGACTCATATAGGGCGGCCGCGAATCGGCACGAGGGTAGTCG CGGAGAAACACTGTTGCGTTACTAACGTTCAGAGGTCTGCGACAGCTTCGATTTGAATGA CTAGCCGGGAACACCAAGTTTCACTGTGTAATTGCGTCCCCCTACTCCGGCGCCTCCTTT GCGACGCTCCCTGGAGAAAAGCACGCCCACTGCACGCGCTCAGTCGCTACTTCCGCTCTC GAGTGTCTCCAAGCAAGATGGCGGAGGAGCCGCAGTCTGTGTTGCAGCTTCCTACTTCAA TTGCTGCTGGAGGGGAAGGACTTACGGATGTCTCCCCAGAAACAACCACCCCGGAGCCCC CGTCTTCCGCTGCAGTTTCCCCGGGAACAGAGGAACCTGCTGGCGACACCAAGAAAAAAA TTGACATTTTGCTAAAGGCTGTGGGAGACACTCCTATTATGAAAACAAAGAAGTGGGCAG TAGAGCGAACACGAACCATCCAAGGACTCATTGACTTCATCAAAAAGTTTCTTAAACTTG TGGCCTCAGAACAGTTGTTTATTTATGTGAATCAGTCCTTTGCTCCTTCCCCAGACCAAG AAGTTGGAACTCTCTATGAGTGTTTTGGCAGTGATGGTAAACTGGTTTTACATTACTGCA AGTCTCAGGCGTGGGGATGAACCACAAAGAANATCAACTTGCTACTACATGAAATGGATT TTCACGGAAGAGACAGCTCTGAAAAGTTTTGATGCTTGTGGCAAGAGACTTAACAGATGT GATCTATTTAGTATGTGTCTACTCTGTGTTTATGCATAAGAAACATCCCTAGCATGAATG GACTCAGAAAATGTGATTTGATTAATGCACCAGTCATCATAAAGATGTCATGATAGTACA C >OriGene 3' read for NM_004707 unedited
TCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAATTCAACATATTTT TATTTGTTAAATATATGAATATAATTAAATATATTCCTTCAGTAAACTGAACTGGACAAA ACTAGAGAACAGACCTTTTCCATACTCATTTAACTTTAAAATGATATTACTTTGAATTCT ACTACATACCATCTTAGATCCTGTTTTGAAATTCCATTCACATTACATTCGATTTACTAG AATGCCTTTTTTTTTCTTTTTTGAGATGGGGTCTCACTCTGTTGCTCAGCAGGAATGCAG TGGTACAATCACGACTCACTATACCCTTGAACTTCTGGGCTCAGGGGATCCTCTTGCCTC TGCAGCCTAATAATTTTTTTTTAATTTTTATTTTTTTGTAGAGTTGGGGATCTTGCCTTG CTAACCAGGCTGGTCTCAAACTTGTGGCTTCACGCAATCCCCACACCTCAGCCTTCCAAA GTGCTGGGATTACAGGTGTAAGCCACCATGCCTGATCTGGAATGCTTTCTAAAAATATGG CATTATAAACATAATACTAAAGAGAAAAAAAGGTTAAATTTCACTTCAAGAAATTATCAG TCTAATAGCATCAGTAAAAATGGCACTTTTAGTCATGGCATAAAATATACAATAAGGCTA TTTTTCAATTATTTGTTTTCAAGTTGGGAAAATGCATTATGAAACACTCAAAAGATGCTA CTGGGGTTACTCATCAAGGGCTTTTGACACCATATAATGACAGAGTAACACCACAGTTAT GTGATGGGGACTCCAGATTCCTTAAATGTTAGGACTGAAACAGGGTTATGACTTATTTGG CCAGAATTATATTTTTCTGTACCCCATTACATATTAAGCCTGTGTATTGGGGTAAATAAC GTCTTCAGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004707 |
| Insert Size | 2260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004707.2, NP_004698.2 |
| RefSeq Size | 2359 bp |
| RefSeq ORF | 564 bp |
| Locus ID | 9140 |
| UniProt ID | O94817 |
| Domains | APG12 |
| Protein Pathways | Regulation of autophagy, RIG-I-like receptor signaling pathway |
| Gene Summary | Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202012 | ATG12 (Myc-DDK-tagged)-Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1 |
CNY 2,400.00 |
|
| RC202012L1 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202012L2 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202012L3 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202012L4 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202012 | ATG12 (tGFP-tagged) - Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1 |
CNY 4,000.00 |
