Apg12 (ATG12) (NM_004707) Human Tagged ORF Clone
CAT#: RG202012
- TrueORF®
ATG12 (tGFP-tagged) - Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1
ORF Plasmid: DDK
"NM_004707" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | APG12; APG12L; FBR93; HAPG12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202012 representing NM_004707
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAGCCGGGAACACCAAGTTTCACTGTGTAATTGCGTCCCCCTACTCCGGCGCCTCCTTTGCGACG CTCCCTGGAGAAAAGCACGCCCACTGCACGCGCTCAGTCGCTACTTCCGCTCTCGAGTGTCTCCAAGCAA GATGGCGGAGGAGCCGCAGTCTGTGTTGCAGCTTCCTACTTCAATTGCTGCTGGAGGGGAAGGACTTACG GATGTCTCCCCAGAAACAACCACCCCGGAGCCCCCGTCTTCCGCTGCAGTTTCCCCGGGAACAGAGGAAC CTGCTGGCGACACCAAGAAAAAAATTGACATTTTGCTAAAGGCTGTGGGAGACACTCCTATTATGAAAAC AAAGAAGTGGGCAGTAGAGCGAACACGAACCATCCAAGGACTCATTGACTTCATCAAAAAGTTTCTTAAA CTTGTGGCCTCAGAACAGTTGTTTATTTATGTGAATCAGTCCTTTGCTCCTTCCCCAGACCAAGAAGTTG GAACTCTCTATGAGTGTTTTGGCAGTGATGGTAAACTGGTTTTACATTACTGCAAGTCTCAGGCGTGGGG A ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202012 representing NM_004707
Red=Cloning site Green=Tags(s) MTSREHQVSLCNCVPLLRRLLCDAPWRKARPLHALSRYFRSRVSPSKMAEEPQSVLQLPTSIAAGGEGLT DVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLK LVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004707 |
ORF Size | 561 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004707.2, NP_004698.2 |
RefSeq Size | 2359 bp |
RefSeq ORF | 423 bp |
Locus ID | 9140 |
UniProt ID | O94817 |
Domains | APG12 |
Protein Pathways | Regulation of autophagy, RIG-I-like receptor signaling pathway |
Gene Summary | Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202012 | ATG12 (Myc-DDK-tagged)-Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1 |
CNY 2,400.00 |
|
RC202012L1 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC202012L2 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC202012L3 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202012L4 | Lenti ORF clone of Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC117215 | ATG12 (untagged)-Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1 |
CNY 2,400.00 |