ATP5MF (NM_004889) Human Untagged Clone
CAT#: SC117080
ATP5J2 (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATP5J2; ATP5JL |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_004889 edited
ATGGCGTCAGTTGGTGAGTGTCCGGCCCCAGTACCAGTGAAGGACAAGAAACTTCTGGAG GTCAAACTGGGGGAGCTGCCAAGCTGGATCTTGATGCGGGACTTCAGTCCTAGTGGCATT TTCGGAGCGTTTCAAAGAGGTTACTACCGGTACTACAACAAGTACATCAATGTGAAGAAG GGGAGCATCTCGGGGATTACCATGGTGCTGGCATGCTACGTGCTCTTTAGCTACTCCTTT TCCTACAAGCATCTCAAGCACGAGCGGCTCCGCAAATACCACTGA >OriGene 5' read for NM_004889 unedited
CCGCGAATTCGGCACGAGCCAAAATGGCGTCAGTTGGTGAGTGTCCGGCCCCAGTGCCAG TGAAGGACAAGAAACTTCTGGAGGTCAAACTGGGGGAGCTGCCAAGCTGGATCTTGATGC GGGACTTCAGTCCTAGTGGCATTTTCGGAGCGTTTCAAAGAGGTTACTACCGGTACTACA ACAAGTACATCAATGTGAAGAAGGGGAGCGATCTCGGGGATTACCATGGTGCTGGCATGC TACGTGCTCTTTAGCTACTCCTTTTCCTACAAGCATCTCAAGCACGAGCGGCTCCGCAAA TACCACTGAAGAGGACACACTCTGCACCCCCCCACCCCACGACCTTGGCCCGAGCCCCTC CGTGAGGAACACAATCTCAATCGTTGCTGAATCCTTTCATATCCTAATAGGAATTAACCT CCAAATAAAACATGACTGGTACGTGTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGC GGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCC AGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAA TTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGCGCTGGCGGGG GGTGGTATGGAGCAAGGGGCAAGTTGGGAACACAACCTGTANGGCCTGCGGGGTCTATGG GGAACCAACTGGAGTGCAG >OriGene 3' read for NM_004889 unedited
AACATTGAATTTAGCTTGCACCCGCGGCCGCATCCTACGATCGGCTTTTTTTTTTTTTTT TTTACACGTACCAGTCATGTTTTATTTGGAGGTTAATTCCTATTAGGATATGAAAGGATT CAGCAACGATTGAGATTGTGTTCCTCACGGAGGGGCTCGGGCCACGGTCGTGGGGTGGGG GGGTGCAGAGTGTGTCCTCTTCAGTGGTATTTGCGGAGCCGCTCGTGCTTGAGATGCTTG TAGGAAAAGGAGTAGCTAAAGAGCACGTAGCATGCCAGCACCATGGTAATCCCCGAGATG CTCCCCTTCTTCACATTGATGTACTTGTTGTAGTACCGGTAGTAACCTCTTTGAAACGCT CCGAAAATGCCACTAGGACTGAAGTCCCGCATCAAGATCCAGCTTGGCAGCTCCCCCAGT TTGACCTCCAGAAGTTTCTTGTCCTTCACTGGTACTGGGGCCGGACACTCACCAACTGAC GCCATTTTGGCTCGTGCCGAATTCCCGGCCGCCCTATACTGAGCCGCATTACCAACTTCT GACGGCTTCACTACACCAGCTTTGCTTCATCAGACCTCCCACCGTACACCCCTACCGGCC ATTTCGCTTAACCGCTCATCGCTAACCCCACCTTTCCCCCCCTCCCCTCTCCCCCCCCCT TCTAACCCCACCCCCTTCCCCCTCCCGCCCTTGCCAATCCGTAATCCCCCCTCCCCCTTC CCCTATTTCCCCCATCTCCCCCCTTCCCCCTACCCGCACCCCCCCCCCCACAGACGCACC TCGCTCATATCTATATCGCGCCTCCCAACCTCCACCCTCGCATCTCCCCTTTTCCTCCTC CCTCCTCTCCTCTCCCCCTCCCCCTCCCCCCCCCTC |
Restriction Sites | NotI-NotI |
ACCN | NM_004889 |
Insert Size | 450 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004889.2, NP_004880.1 |
RefSeq Size | 512 bp |
RefSeq ORF | 285 bp |
Locus ID | 9551 |
UniProt ID | P56134 |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Oxidative phosphorylation |
Gene Summary | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (1) represents the longest transcript, and it encodes the longest isoform (2a). |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202789 | ATP5J2 (Myc-DDK-tagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 1,200.00 |
|
RC202789L3 | Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202789L4 | Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG202789 | ATP5J2 (tGFP-tagged) - Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2,800.00 |