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ATP6V0A1 (NM_005177) Human Untagged Clone
CAT＃: SC116892
ATP6V0A1 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3
CNY 6,096.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | a1; ATP6N1; ATP6N1A; Stv1; Vph1; VPP1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116892 sequence for NM_005177 edited (data generated by NextGen Sequencing)
ATGGGGGAGCTTTTCCGGAGTGAAGAAATGACACTGGCCCAGCTTTTTCTACAGTCAGAG GCTGCTTATTGTTGTGTCAGTGAATTAGGAGAACTTGGAAAGGTTCAGTTTCGTGACTTA AATCCAGATGTGAATGTTTTCCAACGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAA ATGGATCGAAAGCTTCGATTTGTTGAGAAAGAGATAAGAAAAGCTAACATTCCGATTATG GACACCGGTGAAAACCCAGAGGTTCCCTTCCCCCGGGACATGATTGACTTAGAGGCCAAT TTTGAGAAGATTGAAAATGAACTGAAGGAAATCAACACAAACCAGGAAGCTCTGAAGAGA AACTTCCTGGAACTGACCGAATTAAAATTTATACTTCGCAAAACTCAGCAATTTTTTGAT GAGATGGCGGATCCAGACTTGTTGGAAGAGTCCTCATCCCTCTTGGAGCCAAGTGAGATG GGAAGAGGCACTCCTTTAAGACTTGGCTTCGTGGCTGGTGTCATTAACCGGGAGCGCATC CCTACTTTTGAGCGCATGCTTTGGCGGGTATGCCGGGGAAATGTGTTCCTGCGACAGGCT GAAATCGAGAACCCCCTGGAGGATCCTGTGACTGGCGACTACGTGCACAAGTCTGTGTTT ATCATTTTCTTCCAAGGCGATCAGCTGAAAAACAGAGTCAAGAAAATCTGTGAAGGGTTC CGAGCCTCACTCTATCCCTGTCCTGAGACACCACAGGAGAGGAAGGAAATGGCTTCTGGA GTGAATACCAGGATTGATGATCTCCAAATGGTTCTGAATCAAACGGAGGATCACCGCCAG AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGTGTCTGGTTCATCAAAGTGCGGAAGATG AAGGCCATCTATCACACCCTGAACCTGTGCAACATAGATGTGACTCAGAAATGCTTGATT GCAGAGGTCTGGTGCCCTGTCACCGACCTTGACTCCATCCAGTTTGCACTCAGAAGGGGC ACGGAACACAGTGGTTCCACTGTACCTTCCATTTTGAACAGGATGCAGACAAACCAGACT CCCCCAACCTATAACAAAACCAACAAGTTTACCTATGGCTTTCAGAACATAGTAGATGCT TATGGAATTGGAACTTACCGAGAGATAAATCCAGCTCCGTATACTATTATCACGTTCCCT TTTCTATTTGCTGTGATGTTTGGAGACTTCGGTCATGGCATTTTAATGACCCTTTTTGCT GTGTGGATGGTACTGAGGGAGAGCCGGATCCTTTCCCAGAAGAATGAGAATGAGATGTTT AGCACTGTGTTCAGTGGTCGATACATTATTTTATTGATGGGTGTGTTCTCCATGTACACT GGCCTCATCTACAATGATTGCTTTTCCAAGTCTCTTAATATCTTTGGGTCATCCTGGAGT GTACGGCCGATGTTTACTTATAATTGGACTGAAGAGACGCTTCGGGGGAACCCTGTTCTA CAGCTGAACCCAGCCCTCCCTGGAGTGTTTGGTGGACCATACCCTTTTGGCATTGATCCA ATTTGGAACATTGCTACCAATAAACTGACGTTCTTGAACTCCTTTAAGATGAAGATGTCT GTTATCCTTGGTATCATCCATATGCTGTTTGGAGTCAGCCTGAGTCTGTTCAACCATATC TATTTCAAGAAGCCCCTGAATATCTACTTTGGATTTATTCCTGAAATAATCTTCATGACC TCTTTGTTTGGCTATTTGGTTATCCTTATTTTTTACAAGTGGACGGCCTATGATGCTCAT ACCTCTGAGAATGCACCAAGCCTTCTGATCCATTTCATAAACATGTTCCTCTTTTCCTAC CCAGAGTCTGGTTATTCAATGTTGTATTCTGGACAGAAAGGAATTCAGTGTTTCCTGGTA GTGGTTGCACTACTGTGTGTACCTTGGATGCTGCTGTTTAAACCATTGGTCCTTCGCCGT CAGTATTTGAGGAGAAAGCATTTGGGAACTCTCAACTTTGGTGGGATCAGGGTGGGCAAC GGACCGACAGAGGAGGATGCTGAGATTATTCAGCATGACCAGCTCTCCACCCACTCAGAG GACGCAGACGAGTTTGACTTTGGGGACACCATGGTCCACCAGGCCATCCACACCATCGAG TACTGCCTGGGCTGCATCTCCAACACTGCCTCCTACTTGCGGCTCTGGGCCCTCAGCCTC GCTCATGCGCAGCTGTCTGAGGTGCTTTGGACCATGGTGATCCACATCGGCCTGAGCGTG AAGAGCTTGGCGGGAGGTTTGGTGCTGTTCTTCTTCTTCACTGCCTTTGCCACCCTGACC GTGGCCATCCTCCTGATCATGGAGGGCCTCTCGGCCTTTCTCCACGCACTGCGCTTACAC TGGGTTGAGTTCCAGAATAAATTCTACAGCGGGACCGGTTTCAAGTTCTTACCCTTCTCC TTCGAGCATATTCGGGAAGGGAAGTTTGAAGAGTGA Clone variation with respect to NM_005177.3 >OriGene 5' read for NM_005177 unedited
GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGGTTGCTGTGGCGGAGTTTGG AGGTTGGAGAGAAATCCAGGTACTCACTAGACTGGTACCTTCTGCCACCATGGGGGAGCT TTTCCGGAGTGAAGAAATGACACTGGCCCAGCTTTTTCTACAGTCAGAGGCTGCTTATTG TTGTGTCAGTGAATTAGGAGAACTTGGAAAGGTTCAGTTTCGTGACTTAAATCCAGATGT GAATGTTTTCCAACGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAA GCTTCGATTTGTTGAGAAAGAGATAAGAAAAGCTAACATTCCGATTATGGACACCGGTGA AAACCCAGAGGTTCCCTTCCCCCGGGACATGATTGACTTAGAGGCCAATTTTGAGAAGAT TGAAAATGAACTGAAGGAAATCAACACAAACCAGGAAGCTCTGAAGAGAAACTTCCTGGA ACTGACCGAATTAAAATTTATACTTCGCAAAACTCAGCAATTTTTTGATGAGATGGCGGA TCCAGACTTGTTGGAAGAGTCCTCATCCCTCTTGGAGCCAAGTGAGATGGGAAGAGGCAC TCCTTTAAGACTTGGCTTCGTGGCTGGTGTCATTAACCGGGAGCGCATCCCTACTTTTGA GCGCATGCTTTGGCGGGTATGCCGGGGAAATGTGTTCCTGCGACAGGCTGAAATCGAGAA CCCCCTGGAGGATCCTGTGACTGGCGACTACGTGCC >OriGene 3' read for NM_005177 unedited
NNTTTAGCTCTGGNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTCC GGGGAAGTCAAACATACTTTATTCAACATAGGAATGTCTGAACAGGAGGACCTTGGGCAG GGTTCCCTGAACTCTGCTTCAAACCCCACTGGAAACAGAGCAAAGATCATCACGAAAACC CAGGACACCAGGGCAGGGGGGCTGCACAAGGTCGGGTCAGGTCACAGTGGGCCAGCACAC AGTGGCCCCGCCCAGGGCCAGCCCAGCCTGGGAGAGGGGATGAGGGCTCCAGGCAGCTCA CTCAGAAAAGCCAGGGTAGGAGGAGGGCACCAGCCTGGCCCACTCAGGGAATTCTTTCTA CCACTGAAAGCGGTGGGGATTAACTTAGCCAGGGCTGCCACGCCAGCACACGATTACAAG GTTCGGGGTGACAAGGAGGAAGCCCCTTGGCAGGACTGTGCAGCCCTCACAGCCACATGC ACGGCAGCCCTGCCAATGGCTTCCAAACAGCAAACCATCAGTGACACTGACCTTCCCTGC CCAGGACACCCCTCCTGAGACCTGGGGCCAGTGTGGTGAGGAGGGGGCACTAGGGGCGGA GCCAGGCAGGCTGCAGGCAGAACCAAGAGGACCCCAGTCCCACACTGAAGACATCATGAT TGGGGGTGGGGAAGATCTCAGGGTCTCCATCAGGGAAGAGCATCAGTCTGCACCCTCGGC CTGAGGGTGTGCTTGTGGGCAGGGCCCATGNCAGGCCTGNGAAGAAGCCAACCCCGAGAG AGGGAAGCCCTGTGCACTGCGGAGCAGCCAAGGCCTCTGTCCAAGCATCCCNGCTGTGCT ATGTANTGCAGGNCATGAAACGGGAAAGAGGAAAGATGGGAGTGCAGGNNTGGGTCAAAA AAGGCGTGTAAAATCCTATGACCCCTGGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005177 |
| Insert Size | 4230 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005177.2, NP_005168.2 |
| RefSeq Size | 4139 bp |
| RefSeq ORF | 2496 bp |
| Locus ID | 535 |
| UniProt ID | Q93050 |
| Domains | V_ATPase_sub_a |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex
,Tian, S;Wu, Q;Zhou, B;Choi, MY;Ding, B;Yang, W;Dong, M;,
Cell Rep
,PubMed ID 31484065
[ATP6V0A1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223769 | ATP6V0A1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3 |
CNY 6,088.00 |
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| RC223769L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3, Myc-DDK-tagged |
CNY 10,640.00 |
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| RC223769L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3, mGFP tagged |
CNY 10,640.00 |
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| RG223769 | ATP6V0A1 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal V0 subunit a1 (ATP6V0A1), transcript variant 3 |
CNY 7,688.00 |
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