United States
Germany
Japan
United Kingdom
China
CRKL (NM_005207) Human Untagged Clone
CAT＃: SC116881
CRKL (untagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL)
CNY 3,600.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116881 sequence for NM_005207 edited (data generated by NextGen Sequencing)
ATGTCCTCCGCCAGGTTCGACTCCTCGGACCGCTCCGCCTGGTATATGGGGCCGGTGTCT CGCCAGGAGGCGCAGACCCGGCTCCAGGGCCAGCGCCACGGTATGTTCCTCGTCCGCGAT TCTTCCACCTGCCCTGGGGACTATGTGCTGTCGGTGTCCGAGAACTCGCGGGTCTCCCAC TACATCATCAACTCGCTGCCCAACCGCCGTTTTAAGATCGGGGACCAGGAATTTGACCAT TTGCCGGCCCTGCTGGAGTTTTACAAGATCCACTACCTGGACACCACCACCCTCATCGAG CCTGCGCCCAGGTATCCAAGCCCACCAATGGGATCTGTCTCAGCACCCAACCTGCCTACA GCAGAAGATAACCTGGAATATGTACGGACTCTGTATGATTTTCCTGGGAATGATGCCGAA GACCTGCCCTTTAAAAAGGGTGAGATCCTAGTGATAATAGAGAAGCCTGAAGAACAGTGG TGGAGTGCCCGGAACAAGGATGGCCGGGTTGGGATGATTCCTGTCCCTTATGTCGAAAAG CTTGTGAGATCCTCACCACACGGAAAGCATGGAAATAGGAATTCCAACAGTTATGGGATC CCAGAACCTGCTCATGCATACGCTCAACCTCAGACCACAACTCCTCTACCTGCAGTTTCC GGTTCTCCTGGGGCAGCAATCACCCCTTTGCCATCCACACAGAATGGACCTGTCTTTGCG AAAGCAATCCAGAAAAGAGTACCCTGTGCTTATGACAAGACTGCCTTGGCATTAGAGGTT GGTGACATCGTGAAAGTCACAAGGATGAATATAAATGGCCAGTGGGAAGGCGAAGTGAAC GGGCGCAAAGGGCTTTTCCCCTTTACGCACGTCAAAATCTTTGACCCTCAAAACCCAGAT GAAAACGAGTGA Clone variation with respect to NM_005207.3 >OriGene 5' read for NM_005207 unedited
NTGTTAGTTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGCTGCCGCTT CTCCCGCGTCCGCCATTTTGTTGCTGTGGCTATTGGGAACAAGCTGGGCAAAAGCACCCC GGAGGCGCGACGCTCCTTCGAGTTCGGTGCCTCGTGTGACGGCGGGGGTCGGTGAAGACC CGTCGAGCTGCGGCGCCGGCGCGTTCCAGGCCGGGAGTCACTGGAGGCACCCCTGGGACG CCGAGCAGCCCGAGAACCCCGGGGTGGCCTCCGCTGCGGCTCGGGTTTGCCTGCCCCGAC CCCCCGGCTCTGCCGTGCATTCCCGGGCGGCTCTCTCCGTGTGGCGGCCCCGGAGCAGGC GGGCGGCGTCGGAGGATGCTGCGGGCCCGGAGCCGAGAGGAAAGTGCTGGCCCAGCCCTC TGAGCGCTCCTCGAGGTGTGCGAGAGGCCCTTCCTCGGCCCCAAAGCCGTCTGCCGGGCT AAGGCGTGCAGAGCAGGCGAGGACAGCCGCCGCCCCTACCGCCGCAGAGTCCCCGGTCCA ACACCATGTCCTCCGCCAGGTTCGACTCCTCGGACCGCTCCGCCTGGTATATGGGGCCGG TGTCTCGCCAGGAGGCGCAGACCCGGCTCCAGGGCCAGCGCCACGGTATGTTCCTCGTCC GCGATTCTTCCACCTGCCCTGNGGACTATGTGCTGTCGGTGTCCGAGAACTCGCGGGTCT CCCACTACATCATCAACTCGCTGCCCAACCGCCGTTTTAAGATCCGGGACCAGGAATTTG ACCATTTGCCCGGCCCTGCTGGAGTTTTACCAGATCCACTACCTGGACACCACCACCCTC ATCGAGCCTGCGCCCAGGTATCCAAGCCCACCATGGGGATCTGTCTCAGCACCCACCTGC CTACGNAGAAGATACCTGGAAATGTACGACTCTGT >OriGene 3' read for NM_005207 unedited
NNCCCCTTTTTANACCAGAATTCTCTCTTTCCTNAAGTTCACTTCCTGTTGTCCAAANAA AAGCAGATGGCAGCTAAGCCACTGCTTTGTGGAACAGCAAACATGCAGTATCTCGCCATG GCCTCAAGCTTTCTCTGGTGAACACAACAGAATCAGTCCCATGTACAAACGGGCAGCAAT GAGAAGGAAGACCCTTTCCATGTCAACTTCTCTCCACTTGTGTGCTTCCATCAGTATTTC CAGTTTATGATCCAAAAGAACTCTAAAATCGGCTACTCTTTGACAATACGACTATGATAC AAACATCAGGAGGCAGCCGCTGTGTGCATTCCAAATGTGTGTGCGACTGCCAGTCTGCAG AAAGCTGGACTTCGGCAATGCAGTGTGACTTGCCTATGAGAGAAAATGCTTTCCCACTTC AAAGGAGACTAGGACAGGCAGAACAACAAAGCAGCAGGAAACAGGGCAACAGCAATCACT CGTTTTCATCTGGGTTTTGAGGGTCAAAGATTTTGACGTGCGTAAAGGGGAAAAGCCCTT TGCGCCCGTTCACTTCGCCTTCCCACTGGCCATTTATATTCATCCTTGTGACTTTCACGA TGTCACCCACCTCTAATGCCCAAGCAGTCTTGTCATAAGCACAGGGTACTCCTTTCTGGA TTGCTTTCGCAAAGACAGGTCCATTCTGTGTGGGATGCAAAAGGGGTGAATTGCTGCCCC AGGAGAACCCGAAAACTGCAGGGAAAAGGAGTTTGTGGCTGAAGGTGAACCGATTCCTTG AACCGGGTCTGGGGTCCCATTACCGGTGGGATTCCTTTTTCCCTGCCTTCCCGGGGGGGG AGGAACCCCCAAACCTTTCCGCCTTAAGGGGACAGGGATTATCCCCACCCCGGCTTCCTG TGTCCGGGACCTTCCCATTGTTTTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005207 |
| Insert Size | 1910 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005207.2, NP_005198.1 |
| RefSeq Size | 5235 bp |
| RefSeq ORF | 912 bp |
| Locus ID | 1399 |
| UniProt ID | P46109 |
| Domains | SH2, SH3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma |
| Gene Summary | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CRKL protein overexpression enhances cell proliferation and invasion in pancreatic cancer
,Fu, L;Dong, Q;Xie, C;Wang, Y;Li, Q;,
Tumour Biol.
,PubMed ID 25318601
[CRKL]
|
|
CRKL promotes lung cancer cell invasion through ERK-MMP9 pathway
,Lin, F;Chengyao, X;Qingchang, L;Qianze, D;Enhua, W;Yan, W;,
Mol. Carcinog.
,PubMed ID 24664993
[CRKL]
|
|
Clinical significance and biological roles of CRKL in human bladder carcinoma
,Han, B;Luan, L;Xu, Z;Wu, B;,
Tumour Biol. Dec 2013.
,PubMed ID 24375195
[CRKL]
|
|
Genomic and functional analysis identifies CRKL as an oncogene amplified in lung cancer
,Y H Kim, K A Kwei, L Girard, K Salari, J Kao, M Pacyna-Gengelbach, P Wang, T Hernandez-Boussard, A F Gazdar, I Petersen, et al.,
Oncogene (7 December 2009) doi:10.1038/onc.2009.437 Original Article
[CRKL]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208129 | CRKL (Myc-DDK-tagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) |
CNY 3,600.00 |
|
| RC208129L1 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC208129L2 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), mGFP tagged |
CNY 6,000.00 |
|
| RC208129L3 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208129L4 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), mGFP tagged |
CNY 6,000.00 |
|
| RG208129 | CRKL (tGFP-tagged) - Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) |
CNY 5,200.00 |
