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GATA6 (NM_005257) Human Untagged Clone
CAT＃: SC116877
GATA6 (untagged)-Human GATA binding protein 6 (GATA6)
CNY 6,656.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 4,070.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116877 sequence for NM_005257 edited (data generated by NextGen Sequencing)
ATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGAC GCCAGCGACTCCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTCT TCCTCGTCCTCCTCCTGCTCCCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTGC GGGACGCCTCAGCTCGACACGGAGGCGGCGGCCGGACCCCCGGCCCGCTCGCTGCTGCTC AGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGACCTTCGGCGCCTGGGGTCGCG GGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGACCTCGACCAA GCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGCA CCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGCC GCCTACGACGGCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGCG GCGGCGGCCAGCTCCCCGGTCTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCGGC CTACCGTACCACCTGCAGGGGTCGGGCAGTGGGCCAGCCAACCACGCGGGCGGCGCGGGC GCGCACCCCGGCTGGCCTCAGGCCTCGGCCGACAGCCCTCCATACGGCAGCGGAGGCGGC GCGGCTGGCGGCGGGGCCGCGGGGCCTGGCGGCGCTGGCTCAGCCGCGGCGCACGTCTCG GCGCGCTTCCCCTACTCTCCCAGCCCGCCCATGGCCAACGGCGCCGCGCGGGAGCCGGGA GGCTACGCGGCGGCGGGCAGTGGGGGCGCGGGAGGCGTGAGCGGCGGCGGCAGTAGCCTG GCGGCCATGGGCGGCCGCGAGCCCCAGTACAGCTCGCTGTCGGCCGCGCGGCCGCTGAAC GGGACGTACCACCACCACCACCACCACCACCACCACCATCCGAGCCCCTACTCGCCCTAC GTGGGGGCGCCACTGACGCCTGCCTGGCCCGCCGGACCCTTCGAGACCCCGGTGCTGCAC AGCCTGCAGAGCCGCGCCGGAGCCCCGCTCCCGGTGCCCCGGGGTCCCAGTGCAGACCTG CTGGAGGACCTGTCCGAGAGCCGCGAGTGCGTGAACTGCGGCTCCATCCAGACGCCGCTG TGGCGGCGGGACGGCACCGGCCACTACCTGTGCAACGCCTGCGGGCTCTACAGCAAGATG AACGGCCTCAGCCGGCCCCTCATCAAGCCGCAGAAGCGCGTGCCTTCATCACGGCGGCTT GGATTGTCCTGTGCCAACTGTCACACCACAACTACCACCTTATGGCGCAGAAACGCCGAG GGTGAACCCGTGTGCAATGCTTGTGGACTCTACATGAAACTCCATGGGGTGCCCAGACCA CTTGCTATGAAAAAAGAGGGAATTCAAACCAGGAAACGAAAACCTAAGAACATAAATAAA TCAAAGACTTGCTCTGGTAATAGCAATAATTCCATTCCCATGACTCCAACTTCCACCTCT TCTAACTCAGATGATTGCAGCAAAAATACTTCCCCCACAACACAACCTACAGCCTCAGGG GCGGGTGCCCCGGTGATGACTGGTGCGGGAGAGAGCACCAATCCCGAGAACAGCGAGCTC AAGTATTCGGGTCAAGATGGGCTCTACATAGGCGTCAGTCTCGCCTCGCCGGCCGAAGTC ACGTCCTCCGTGCGACCGGATTCCTGGTGCGCCCTGGCCCTGGCCTGA Clone variation with respect to NM_005257.3 >OriGene 5' read for NM_005257 unedited
GGTCCTTACACCCGCCCCGTTGACTCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTAT ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG GCCGCGAATTCGGCACGAGGCTTCTCTCCGCGCCTGGGAGCACCCTCCGCCGCGGCCGTT CTCCATGCGCAGCGCCCGCCCGAGGAGCTAGACGTCAGCTTGGAGCGGCGCCGGACCGTG GATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGA CGCCAGCGACTCCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTC TTCCTCGTCCTCCTCCTGCTCCCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTG CGGGACGCCTCAGCTCGACACGGAGGCGGCGGCCGGACCCCCGGCCCGCTCGCTGCTGCT CAGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGACCTTCGGCGCCTGGGGTCGC GGGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGACCTCGACCA AGCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGC ACCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGC CGCCTACGACGGCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGC GGCGGCGGCCAGCTCCCCAGTCTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCAG CCTACCGTACCCCTGCAGGGGTCGGGCAGTGGGCCAGCCAACACGCGGGCGGGCGCGGCG CGCACCCAGCTGGNCTCATGCCTCAGCCGACAGCCTC >OriGene 3' read for NM_005257 unedited
TAGCTNTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAC AGAAAACAAAACAATACCCTTTTTATTTTTAAAACTCTGGTGAAATAGGTTTCCATAGGA CATCCCATTCAAAAAAAAAAATTAATGTAAACCAACCTGCCTGTGGGTTAGTCACACTAT ACAGACTTCATCAGAGTGTTTACAGATACCTAGAGTATTTACAAATGCTATCCGTTAGGT AGTAAAGTGTTGTTGCAATTTTTCCAGCACATATTCCACAGTAAAATAAAGGGGGTATAT TGGTGTTCTTGTATAAAAATTCTTAACAAATAGAGAAAGGCAATTTTCCTGAGAGAAGAT TTGTTCTTGAAATATTTTTCTTTAACAATTGGAAATATAAAATGGGATGTAGTTGTAAAA GAAGCATAAGAATGTCTAAGTTAACATTTTTCTTTTAAATTATTTTAAGTACCATTAAGT TAAAATGTTTACATTCATTATAAAAAATGCTGTTAAATCTTATGCAAATGATTTAACACT GATTGCTGCAACGCAAATTGCACTTTTTAAAAAAATTATTGTATACATGGAAGAAAAATA TCTTGTTTGCTACCCTGTTTACAAATTCCGACTGACTTCAGATCAGCCACACAATATGAA CTTTGAGAAACGTCAGTCACAGTGTATGTTAAAGTTACAATGCAGTCTTGCAAACTGAGT AATATTTTTGATAAGAGACCTCATGAACCGACTCAGCCTCCAGAGATGTGTACCAAATGG CCTCAAGTGATCTTCTCCATGAACGCACATGAAATCTGGATGTGGNAAAGGTATGAAGAA ATTCTGGNAACACCAAATTGATCAGAAGAAGCCATGATTTTGGACAAATGCAAAATCTTT TTAAAAACTCTCTCATATTCATAGCAGCAGGGCCAGTCTAGTCTGCTTCTGAAGTGGACA GGTCCGCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005257 |
| Insert Size | 3100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005257.3, NP_005248.2 |
| RefSeq Size | 3494 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 2627 |
| UniProt ID | Q92908 |
| Domains | GATA, GATA-N |
| Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs
,Simeonov, KP;Uppal, H;,
PLoS ONE
,PubMed ID 24963715
[GATA6]
|
|
A novel mutation in GATA6 causes pancreatic agenesis
,Stanescu, DE;Hughes, N;Patel, P;De León, DD;,
Pediatr Diabetes, Jan 2014.
,PubMed ID 24433315
[GATA6]
|
|
GATA-6 and NF-B Activate CPI-17 Gene Transcription and Regulate Ca2+ Sensitization of Smooth Muscle Contraction
,Ettickan Boopathi, Joseph A. Hypolite, Stephen A. Zderic, Cristiano Mendes Gomes, Bruce Malkowicz, Hsiou-Chi Liou, Alan J. Wein, and Samuel Chacko,
Mol. Cell. Biol., Mar 2013; 33: 1085 - 1102.
[GATA6]
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Resources
Other Versions
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| RC220721L2 | Lenti-ORF clone of GATA6 (mGFP-tagged)-Human GATA binding protein 6 (GATA6) |
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| RC220721L3 | Lenti-ORF clone of GATA6 (Myc-DDK-tagged)-Human GATA binding protein 6 (GATA6) |
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| RC220721L4 | Lenti-ORF clone of GATA6 (mGFP-tagged)-Human GATA binding protein 6 (GATA6) |
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| RG220721 | GATA6 (tGFP-tagged) - Human GATA binding protein 6 (GATA6) |
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