DDOST (NM_005216) Human Untagged Clone
CAT#: SC116849
DDOST (untagged)-Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST)
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AGER1; CDG1R; GATD6; OKSWcl45; OST; OST48; WBP1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_005216, the custom clone sequence may differ by one or more nucleotides
ATGGGGTACTTCCGGTGTGCAGGTGCTGGGTCCTTCGGCAGGAGGAGGAAGATGGAGCCCAGCACCGCGG CCCGGGCTTGGGCCCTCTTTTGGTTGCTGCTGCCCTTGCTTGGCGCGGTTTGCGCCAGCGGACCCCGCAC CTTAGTGCTGCTGGACAACCTCAACGTGCGGGAGACTCATTCGCTTTTCTTCCGGAGCCTGAAGGACCGG GGCTTTGAGCTCACATTCAAGACCGCTGATGACCCCAGCCTGTCTCTCATAAAGTATGGGGAATTCCTCT ATGACAATCTCATCATTTTCTCCCCTTCGGTAGAAGATTTTGGAGGCAACATCAACGTGGAGACCATCAG TGCCTTTATTGACGGCGGAGGCAGTGTGCTGGTAGCTGCCAGCTCCGACATTGGTGACCCTCTTCGAGAG CTGGGCAGTGAGTGCGGGATTGAGTTTGACGAGGAGAAAACGGCTGTCATTGACCATCACAACTATGACA TCTCAGACCTTGGCCAGCATACGCTCATCGTGGCTGACACTGAGAACCTGCTGAAGGCCCCAACCATCGT TGGGAAATCATCTCTAAATCCCATCCTCTTTCGAGGTGTTGGGATGGTGGCCGATCCTGATAACCCTTTG GTGCTGGACATCCTGACGGGCTCTTCCACCTCTTACTCCTTCTTCCCGGACAAGCCTATCACCCAGTATC CACATGCGGTGGGGAAGAACACCCTCCTCATTGCTGGGCTCCAGGCCAGGAACAATGCCCGCGTCATCTT CAGCGGCTCCCTCGACTTCTTCAGCGACTCCTTCTTCAACTCAGCAGTGCAGAAGGCGGCGCCCGGCTCC CAGAGGTATTCCCAGACAGGCAACTATGAACTAGCTGTGGCCCTCTCCCGCTGGGTGTTCAAGGAGGAGG GTGTCCTCCGTGTGGGGCCTGTGTCCCATCATCGGGTGGGCGAGACAGCCCCACCCAATGCCTACACTGT CACTGACCTAGTGGAGTATAGCATCGTGATCCAGCAGCTCTCAAATGGCAAATGGGTCCCCTTTGATGGC GATGACATTCAGCTGGAGTTTGTCCGCATTGATCCTTTTGTGAGGACCTTCCTGAAGAAGAAAGGTGGCA AATACAGTGTTCAGTTCAAGTTGCCCGACGTGTATGGTGTATTCCAGTTTAAAGTGGATTACAACCGGCT AGGCTACACACACCTGTACTCTTCCACTCAGGTATCCGTGCGGCCACTCCAGCACACGCAGTATGAGCGC TTCATCCCCTCGGCCTACCCCTACTACGCCAGCGCCTTCTCCATGATGCTGGGGCTCTTCATCTTCAGCA TCGTCTTCTTGCACATGAAGGAGAAGGAGAAGTCCGACTGA >OriGene 5' read for NM_005216 unedited
CACGAGGACGCCCAGCCGCGGCGTATCCCGATCACTTCCGGGTAGTGCTCCACGGGCACG AGCCGCGATTGGGCTACCGTAGATGGGGTACTTCCGGTGTGCAGGTGCTGGGTCCTTCGG CAGGAGGAGGAAGATGGAGCCCAGCACCGCGGCCCGGGCTTGGGCCCTCTTTTGGTTGCT GCTGCCCTTGCTTGGCGCGGTTTGCGCCAGCGGACCCCGCACCTTAGTGCTGCTGGACAA CCTCAACGTGCGGGAGACTCATTCGCTTTTCTTCCGGAGCCTGAAGGACCGGGGCTTTGA GCTCACATTCAAGACCGCTGATGACCCCAGCCTGTCTCTCATAAAGTATGGGGAATTCCT CTATGACAATCTCATCATTTTCTCCCCTTCGGTAGAAGATTTTGGAGGCAACATCAACGT GGAGACCATCAGTGCCTTTATTGACGGCGGAGGCAGTGTGCTGGTAGCTGCCAGCTCCGA CATTGGTGACCCTCTTCGAGAGCTGGGCAGTGAGTGCGGGATTGAGTTTGACGAGGAGAA AACGGCTGTCATTGACCATCACAACTATGACATCTCAGACCTTGGCCAGCATACGCTCAT CGTGGCTGACACTGAGAACCTGCTGAAGGCCCCCACCATCNNGTGGAAATCATCT >OriGene 3' read for NM_005216 unedited
NAACGTACACTATGNACCGCGGCCCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTT TATTTATTTATTATTTATTTATTTATTTATTTATTTATTTATTTATTTATATTTGAGACA GAGTCTTAACACTGTTGCCCAAGCTGGAGTGCAATGGCGTGATCTCAGCTCACTGCAAGC TCTGCCTCTTGGATTCATGCCTTTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACCACAG GTGCCCACCACCACGCCCAGCCAATTTTTTGTACTTTTAGTAGAGACAGGGTTTTACCGT GTTAGCCAGGATAGTCTCGATCTCCTGACCTCGTGAGCCGCCTGCCTCGGCCTCCCAAAG TGCTGGGATTACAGGCATGAGCCACCGTGCCTGGCCACGTCCCTATTTTAGAAATGAGAG GAGTGACTGCACATAGGAAAAATGCCACTTTTAGCAATTCAAAGTGGAAAAACTTCTTTT ATATAAAAATTATCCCAACTCCCACCCCTTGGCTCTCAGTGTTGCATCTCCCACAGAGGT AAAGTTGTGCCATTTTCCCACGGCTTTAAACAAAGCAAAACAAAACCACCAATCCTAATA ACCCCCCTCCCTGCCCCGTCTCCACGCTGTGCGGAGAGGGCTCTAGCCCCTCAGTCGGAC TTCTCCTTCTCCTTCATGTGCAAGAAGACGATGCTGAAGATGAAGAGCCCCAGCATCATG GATTATGCGCTGGCGTAATATGGGTAGGCCCGAGGGGATGAAACGCCTCATACTGGCTGT GCCTGGAATGGCCCCACGGGATACCTGAGTGGGAGAGTACAGGTGGTGGCTAGCCCTACC CCGTTTGTATCCCCCTT |
Restriction Sites | NotI-NotI |
ACCN | NM_005216 |
Insert Size | 2000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005216.3, NP_005207.2 |
RefSeq Size | 2144 bp |
RefSeq ORF | 1371 bp |
Locus ID | 1650 |
UniProt ID | P39656 |
Domains | DDOST_48kD |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
Gene Summary | This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200672 | DDOST (Myc-DDK-tagged)-Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST) |
CNY 3,656.00 |
|
RC200672L1 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC200672L2 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), mGFP tagged |
CNY 5,890.00 |
|
RC200672L3 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200672L4 | Lenti ORF clone of Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST), mGFP tagged |
CNY 5,890.00 |
|
RG200672 | DDOST (tGFP-tagged) - Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST) |
CNY 5,256.00 |