DDOST (NM_005216) Human Untagged Clone

CAT#: SC116849

DDOST (untagged)-Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase (DDOST)



  "NM_005216" in other vectors (6)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
DDOST mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AGER1; CDG1R; GATD6; OKSWcl45; OST; OST48; WBP1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_005216, the custom clone sequence may differ by one or more nucleotides


ATGGGGTACTTCCGGTGTGCAGGTGCTGGGTCCTTCGGCAGGAGGAGGAAGATGGAGCCCAGCACCGCGG
CCCGGGCTTGGGCCCTCTTTTGGTTGCTGCTGCCCTTGCTTGGCGCGGTTTGCGCCAGCGGACCCCGCAC
CTTAGTGCTGCTGGACAACCTCAACGTGCGGGAGACTCATTCGCTTTTCTTCCGGAGCCTGAAGGACCGG
GGCTTTGAGCTCACATTCAAGACCGCTGATGACCCCAGCCTGTCTCTCATAAAGTATGGGGAATTCCTCT
ATGACAATCTCATCATTTTCTCCCCTTCGGTAGAAGATTTTGGAGGCAACATCAACGTGGAGACCATCAG
TGCCTTTATTGACGGCGGAGGCAGTGTGCTGGTAGCTGCCAGCTCCGACATTGGTGACCCTCTTCGAGAG
CTGGGCAGTGAGTGCGGGATTGAGTTTGACGAGGAGAAAACGGCTGTCATTGACCATCACAACTATGACA
TCTCAGACCTTGGCCAGCATACGCTCATCGTGGCTGACACTGAGAACCTGCTGAAGGCCCCAACCATCGT
TGGGAAATCATCTCTAAATCCCATCCTCTTTCGAGGTGTTGGGATGGTGGCCGATCCTGATAACCCTTTG
GTGCTGGACATCCTGACGGGCTCTTCCACCTCTTACTCCTTCTTCCCGGACAAGCCTATCACCCAGTATC
CACATGCGGTGGGGAAGAACACCCTCCTCATTGCTGGGCTCCAGGCCAGGAACAATGCCCGCGTCATCTT
CAGCGGCTCCCTCGACTTCTTCAGCGACTCCTTCTTCAACTCAGCAGTGCAGAAGGCGGCGCCCGGCTCC
CAGAGGTATTCCCAGACAGGCAACTATGAACTAGCTGTGGCCCTCTCCCGCTGGGTGTTCAAGGAGGAGG
GTGTCCTCCGTGTGGGGCCTGTGTCCCATCATCGGGTGGGCGAGACAGCCCCACCCAATGCCTACACTGT
CACTGACCTAGTGGAGTATAGCATCGTGATCCAGCAGCTCTCAAATGGCAAATGGGTCCCCTTTGATGGC
GATGACATTCAGCTGGAGTTTGTCCGCATTGATCCTTTTGTGAGGACCTTCCTGAAGAAGAAAGGTGGCA
AATACAGTGTTCAGTTCAAGTTGCCCGACGTGTATGGTGTATTCCAGTTTAAAGTGGATTACAACCGGCT
AGGCTACACACACCTGTACTCTTCCACTCAGGTATCCGTGCGGCCACTCCAGCACACGCAGTATGAGCGC
TTCATCCCCTCGGCCTACCCCTACTACGCCAGCGCCTTCTCCATGATGCTGGGGCTCTTCATCTTCAGCA
TCGTCTTCTTGCACATGAAGGAGAAGGAGAAGTCCGACTGA


>OriGene 5' read for NM_005216 unedited
CACGAGGACGCCCAGCCGCGGCGTATCCCGATCACTTCCGGGTAGTGCTCCACGGGCACG
AGCCGCGATTGGGCTACCGTAGATGGGGTACTTCCGGTGTGCAGGTGCTGGGTCCTTCGG
CAGGAGGAGGAAGATGGAGCCCAGCACCGCGGCCCGGGCTTGGGCCCTCTTTTGGTTGCT
GCTGCCCTTGCTTGGCGCGGTTTGCGCCAGCGGACCCCGCACCTTAGTGCTGCTGGACAA
CCTCAACGTGCGGGAGACTCATTCGCTTTTCTTCCGGAGCCTGAAGGACCGGGGCTTTGA
GCTCACATTCAAGACCGCTGATGACCCCAGCCTGTCTCTCATAAAGTATGGGGAATTCCT
CTATGACAATCTCATCATTTTCTCCCCTTCGGTAGAAGATTTTGGAGGCAACATCAACGT
GGAGACCATCAGTGCCTTTATTGACGGCGGAGGCAGTGTGCTGGTAGCTGCCAGCTCCGA
CATTGGTGACCCTCTTCGAGAGCTGGGCAGTGAGTGCGGGATTGAGTTTGACGAGGAGAA
AACGGCTGTCATTGACCATCACAACTATGACATCTCAGACCTTGGCCAGCATACGCTCAT
CGTGGCTGACACTGAGAACCTGCTGAAGGCCCCCACCATCNNGTGGAAATCATCT
>OriGene 3' read for NM_005216 unedited
NAACGTACACTATGNACCGCGGCCCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTT
TATTTATTTATTATTTATTTATTTATTTATTTATTTATTTATTTATTTATATTTGAGACA
GAGTCTTAACACTGTTGCCCAAGCTGGAGTGCAATGGCGTGATCTCAGCTCACTGCAAGC
TCTGCCTCTTGGATTCATGCCTTTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACCACAG
GTGCCCACCACCACGCCCAGCCAATTTTTTGTACTTTTAGTAGAGACAGGGTTTTACCGT
GTTAGCCAGGATAGTCTCGATCTCCTGACCTCGTGAGCCGCCTGCCTCGGCCTCCCAAAG
TGCTGGGATTACAGGCATGAGCCACCGTGCCTGGCCACGTCCCTATTTTAGAAATGAGAG
GAGTGACTGCACATAGGAAAAATGCCACTTTTAGCAATTCAAAGTGGAAAAACTTCTTTT
ATATAAAAATTATCCCAACTCCCACCCCTTGGCTCTCAGTGTTGCATCTCCCACAGAGGT
AAAGTTGTGCCATTTTCCCACGGCTTTAAACAAAGCAAAACAAAACCACCAATCCTAATA
ACCCCCCTCCCTGCCCCGTCTCCACGCTGTGCGGAGAGGGCTCTAGCCCCTCAGTCGGAC
TTCTCCTTCTCCTTCATGTGCAAGAAGACGATGCTGAAGATGAAGAGCCCCAGCATCATG
GATTATGCGCTGGCGTAATATGGGTAGGCCCGAGGGGATGAAACGCCTCATACTGGCTGT
GCCTGGAATGGCCCCACGGGATACCTGAGTGGGAGAGTACAGGTGGTGGCTAGCCCTACC
CCGTTTGTATCCCCCTT
Restriction Sites NotI-NotI     
ACCN NM_005216
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005216.3, NP_005207.2
RefSeq Size 2144 bp
RefSeq ORF 1371 bp
Locus ID 1650
UniProt ID P39656
Domains DDOST_48kD
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
Gene Summary This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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