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GTF2H1 (NM_005316) Human Untagged Clone
CAT＃: SC116821
GTF2H1 (untagged)-Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1
CNY 4,088.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BTF2; P62; TFB1; TFIIH |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116821 sequence for NM_005316 edited (data generated by NextGen Sequencing)
ATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAAAAGAAGCAG GATGGAGCTCTGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGCAAAGATAGA TTTACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATTAGTCCAGAAGGAAAA GCTAAAATTCAGCTTCAGCTGGTCCTACATGCAGGGGACACAACTAACTTCCATTTTTCC AATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAGACCTTCTTCAGCAGCTGCTG CCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAATGCTGCAAGAA GATCCTGTTTTGTTTCAGCTTTATAAAGACCTTGTTGTGAGTCAAGTGATCAGTGCTGAG GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTTCCACATCCAATCAT AAGCAGGATGTTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCCAAACTGATGGC TGTAACGGTCTAAGATATAATTTAACTTCTGATATCATTGAGTCCATATTTAGGACCTAT CCAGCAGTAAAAATGAAATATGCAGAAAATGTTCCCCACAACATGACAGAGAAGGAATTC TGGACACGTTTTTTCCAGTCCCATTATTTTCACAGGGATCGGCTGAATACAGGGTCAAAG GATCTCTTTGCAGAATGTGCCAAAATAGATGAAAAAGGCCTAAAAACAATGGTTTCATTA GGAGTGAAAAACCCACTACTAGATTTAACAGCTTTGGAAGATAAACCATTAGATGAGGGC TATGGCATTTCCTCTGTGCCATCTGCTTCCAATTCTAAATCCATAAAAGAGAATAGTAAT GCTGCCATCATCAAGAGATTTAACCATCACAGTGCCATGGTCCTGGCAGCTGGACTCAGA AAACAAGAAGCACAAAATGAACAAACTAGTGAGCCCAGCAACATGGATGGAAATTCCGGA GATGCAGACTGCTTTCAGCCAGCAGTCAAAAGGGCGAAATTACAAGAGTCCATTGAATAT GAAGACTTGGGGAAAAATAATTCTGTAAAAACGATTGCACTAAACCTCAAGAAGTCAGAT AGGTATTATCATGGTCCAACTCCAATCCAGTCACTACAGTATGCAACAAGTCAGGACATT ATTAATTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTATACACCCAAGTTAACTCAG GTTCTCTCAAGTAGTGCTGCCAGTAGTACCATCACAGCACTGTCACCTGGAGGGGCACTT ATGCAGGGAGGAACACAGCAAGCCATAAACCAGATGGTGCCAAATGATATTCAATCTGAA TTGAAACACTTATATGTAGCTGTTGGAGAACTTCTACGACATTTCTGGTCCTGCTTTCCT GTTAATACGCCATTCCTAGAAGAAAAGGTAGTGAAAATGAAAAGTAATTTGGAACGATTC CAAGTTACGAAGCTCTGTCCATTCCAAGAAAAGATTCGGAGACAGTATTTAAGCACAAAT TTGGTAAGTCACATAGAAGAGATGCTCCAGACAGCCTACAACAAGCTCCACACATGGCAG TCACGGCGTCTGATGAAGAAAACGTGA Clone variation with respect to NM_005316.3 >OriGene 5' read for NM_005316 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGAAGGTGTCATGGC GGCCGCGCTCCTGAGTCACGTGCCCAGGGCCCGCCTTGCTACTTCCGGTCACGTGCCCTC AGACTCCTCGCAGCCAGCGATGGAGGCGAGACCCCCTAGTAACAGAGGCGGTGGCTACTG CTGCGGCCACTGGGTTTCGGCCTCTTCCCAGCAGCGGCTCTAAGAAGCGCAGCGGAACTC GACCGGATCCAACCCAGTTAGTTACTTCCTGTCTAGAGTTGTAGCTTCCACCTGCACCTT CTAGCCACCATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAA AAGAAGCAGGATGGAGCTCTGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGC AAAGATAGATTTACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATTAGTCCA GAAGGAAAAGCTAAAATTCAGCTTCAGCTGGTCCTACATGCAGGGGACACAACTAACTTC CATTTTTCCAATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAGACCTTCTTCAG CAGCTGCTGCCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAATG CTGCAAGAAGATCCTGTTTTGTTTCAGCTNTATAAAGACCTTGTTGTGAGTCAAGTGATC AGTGCTGAGGAATTCTGGGCCAATCNGTTAATGTGAAATGCACAGNNATAGTCTTCACAT NCAATCATAAGCANGATGTTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCAACT GATGGCTGTAACGGNNCTAGAATAANTTAACTCTGATATCATGAGTCATATTTNAGACCT ATCCGCAGTAAAAG >OriGene 3' read for NM_005316 unedited
GACCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAGGAGAAACATCTTTT ATTTCTTCTAAAAGGTGAAAATAAGATTTTTTTAATATTTCTCACTCATAAGATTTTTAA ACTCTTAAAAATGCAATATCTTCTTTTCAAAGCACATGCCATCTTAAAAAAATCTTAGCA ATGTACATTTGCACTCAAAGAAAAACATGCAGCGCCATTGTCTTCTGACAAAATTCTGCA TTAAAAAACCCTATGCGACTTTCTGCAAATATATTGCCTCTTTATTATTCAGGGACTCCA AACCACAAACATTCAGTGCAATATTTATCGTTTCTTATTTCCGTAGGAAACACAGAACCA ATGATGTCTTCTGACAACTGTTTCCTGATGGTTGAGCAACAGTCTCCCTTTGCTTTATTT TAAAGTACTTTTTTTAACTTGCTGATGTAAGGTATAATTGGAATTCATTAAAACTTGAGG ATCCAATGGAGGGTGAAGAATGAAGGATGGAGGAGGAATACCTTAAGGTCTACCCTGTGC CATAAACTTACGAAAAAATGTCCACCGAAAAAACGACACCCAAAAAACCTCAGATTTTAT CAAAGCCCCCTTTAAAAAGTGTTTTTTTAACTTCTGAAACACCCTAATGTGAATCAAAAG CACGCCCTGACCAACTGCAGTAAACAGTCTTTTAAAATATATATGTCCATACCCATGCTC ATATCTTGCTGGGGGCGTATATATATATATTCGCCACCTAACCCCATTGGGTTCTGTCTT AATTACCCCCACCACCATTTAACCCCTTCTTTCCTTTCACGCCTACAAGGGCCAAGACCA ATACCATCTATTTCTGGGAGTTGGGCGGCAACATAAGTTTCTTATAACCTCTGCGAGGCC ACTCGCTTTGGCTTGCAGCCCAGCTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005316 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005316.2, NP_005307.1 |
| RefSeq Size | 2989 bp |
| RefSeq ORF | 1647 bp |
| Locus ID | 2965 |
| UniProt ID | P32780 |
| Domains | BSD |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Basal transcription factors, Nucleotide excision repair |
| Gene Summary | Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this RefSeq transcript is supported by transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201341 | GTF2H1 (Myc-DDK-tagged)-Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1 |
CNY 4,080.00 |
|
| RC201341L1 | Lenti ORF clone of Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1, Myc-DDK-tagged |
CNY 6,480.00 |
|
| RC201341L2 | Lenti ORF clone of Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC201341L3 | Lenti ORF clone of Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC201341L4 | Lenti ORF clone of Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG201341 | GTF2H1 (tGFP-tagged) - Human general transcription factor IIH, polypeptide 1, 62kDa (GTF2H1), transcript variant 1 |
CNY 5,680.00 |
