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n-Myc (MYCN) (NM_005378) Human Untagged Clone
CAT＃: SC116780
MYCN (untagged)-Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN)
CNY 5,488.00
| Cited in 7 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe37; MODED; N-myc; NMYC; ODED |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116780 sequence for NM_005378 edited (data generated by NextGen Sequencing)
ATGCCGAGCTGCTCCACGTCCACCATGCCGGGCATGATCTGCAAGAACCCAGACCTCGAG TTTGACTCGCTACAGCCCTGCTTCTACCCGGACGAAGATGACTTCTACTTCGGCGGCCCC GACTCGACCCCCCCGGGGGAGGACATCTGGAAGAAGTTTGAGCTGCTGCCCACGCCCCCG CTGTCGCCCAGCCGTGGCTTCGCGGAGCACAGCTCCGAGCCCCCGAGCTGGGTCACGGAG ATGCTGCTTGAGAACGAGCTGTGGGGCAGCCCGGCCGAGGAGGACGCGTTCGGCCTGGGG GGACTGGGTGGCCTCACCCCCAACCCGGTCATCCTCCAGGACTGCATGTGGAGCGGCTTC TCCGCCCGCGAGAAGCTGGAGCGCGCCGTGAGCGAGAAGCTGCAGCACGGCCGCGGGCCG CCAACCGCCGGTTCCACCGCCCAGTCCCCGGGAGCCGGCGCCGCCAGCCCTGCGGGTCGC GGGCACGGCGGGGCTGCGGGAGCNGGCCGCGCCNNNNNNNNNCTGCCCGCCGAGCTCGCC CACCCGGCCGCCGAGTGCGTGGATCCCGCCGTGGTCTTCCCNNNNNNNGTGAACAAGCGC GAGCCAGCGCCCGTGCCCGCAGCCCCGGCCAGTGCCCCGGCGGCGGGCCCTGCGGTCGCC TCGGGGGCGGGTATTGCCGCCCCAGCCGGGGCCCCGGGGGTCGCCCCTCCGCGCCCAGGC GGCCGCCAGACCAGCGGCGGCGACCACAAGGCCCTCAGTACCTCCGGAGAGGACACCCTG AGCGATTCAGATGATGAAGATGATGAAGAGGAAGATGAAGAGGAAGAAATCGACGTGGTC ACTGTGGAGAAGCGGCGTTCCTCCTCCAACACCAAGGCTGTCACCACATTCACCATCACT GTGCGTCCCAAGAACGCAGCCCTGGGTCCCGGGAGGGCTCAGTCCAGCGAGCTGATCCTC AAACGATGCCTTCCCATCCACCAGCAGCACAACTATGCCGCCCCCTCTCCCTACGTGGAG AGTGAGGATGCACCCCCACAGAAGAAGATAAAGAGCGAGGCGTCCCCACGTCCGCTCAAG AGTGTCATCCCCCCAAAGGCTAAGAGCTTGAGCCCCCGAAACTCTGACTCGGAGGACAGT GAGCGTCGCAGAAACCACAACATCCTGGAGCGCCAGCGCCGCAACGACCTTCGGTCCAGC TTTCTCACGCTCAGGGACCACGTGCCGGAGTTGGTAAAGAATGAGAAGGCCGCCAAGGTG GTCATTTTGAAAAAGGCCACTGAGTATGTCCACTCCCTCCAGGCCGAGGAGCACCAGCTT TTGCTGGAAAAGGAAAAATTGCAGGCAAGACAGCAGCAGTTGCTAAAGAAAATTGAACAC GCTCGGACTTGCTAG Clone variation with respect to NM_005378.4 504 c=>n;514 g=>n;515 g=>n;516 g=>n;517 g=>n;518 c=>n;519 c=>n;520 g=>n;521 c=>n;522 c=>n;582 c=>n;583 t=>n;584 t=>n;585 t=>n;586 c=>n;587 c=>n;588 c=>n >OriGene 5' read for NM_005378 unedited
ATTAGGTCTACGACTCACTATAGGCGGCNCGCGAATCGGCACGAGGGTCGGTTGCAGTGT TGGAGGTCGGCGCCGGCCCCCGCCTTCCGCGCCCCCCACGGGAAGGAAGCACCCCCGGTA TTAAAACGAACGGGGCGGAAAGAAGCCCTCAGTCGCCGGCCGGGAGGCGAGCCGATGCCG AGCTGCTCCACGTCCACCATGCCGGGCATGATCTGCAAGAACCCAGACCTCGAGTTTGAC TCGCTACAGCCCTGCTTCTACCCGGACGAAGATGACTTCTACTTCGGCGGCCCCGACTCG ACCCCCCCGGGGGAGGACATCTGGAAGAAGTTTGAGCTGCTGCCCACGCCCCCGCTGTCG CCCAGCCGTGGCTTCGCGGAGCACAGCTCCGAGCCCCCGAGCTGGGTCACGGAGATGCTG CTTGAGAACGAGCTGTGGGGCAGCCCGGCCGAGGAGGACGCGTTCGGCCTGGGGGGACTG GGTGGCCTCACCCCCAACCCGGTCATCCTCCAGGACTGCATGTGGAGCGGCTTCTCCGCC CGCGAGAAGCTGGAGCGCGCCGTGAGCGAGAAGCTGCAGCACGGCCGCGGGCCGCCAACC GCCGGTTTCACCGCCCAGTCCCCCGGGAGCCGGCGCCGCCAGCCCTGCGGGTCGCGGGCA CGGTGGGGCTGCGGGAGCCGGCCGCGCCGGGGCCGACCTGCCCGCCGAGCTCGCCCACCC GGCCCGCGAGTGCGCGGATCCCGCCGAGGTCTTCCCCTTTCCTGTGAACAAGCGCGAGCC CGCGCCCGTGCCCGCATCCCCGCCCATGCCCCGGCGGCGGGCCCCTGCGGCGCCTTGGGG GCGGGTTATGACGCCCCAGCCGGGCCCCGAGGGTTGCCCCTCCCTGCCAGCGGCCGCCAA AAATCGTCGGCGACCCCAGGCCCTTGATCACTCGGCGAAGAACACTTGAGCGCATCTTAG CTCATATATATCCACGCAACCCGAC >OriGene 3' read for NM_005378 unedited
NTTACCTTGGACGCGGCCGCATTCTANGATCGAGGTTTTTTTTTTTTTTTTTTTTTTTGC ATCATTTAATTTTAAGCTATTTATTTTCATAAACATGAGGTATTTCAAAGTGCTATAAGA TGCAGCACTAAATATATACATTTTGAAGAAATTAAACACAGAACTTTGCATTTACCCAGT TCTATGCACCAAACATGAACAAATACATTAACAGGAAGAAACAGGCTAGGAAAAAGGCAT ATATATATAGTAAATTTCTTTACAAAAGTTTCTTAGTTCAAAAAGTGATAAAGTAATATC TACTCAAAACTTTCACAACTCATTTTCATACGAAAATATAAGTATCAAATTTAGTTATGT ATCAGCGTCATACTAAAGTATACAGGCAGTGTAAGAATTAGTACAGTACATAACAGAGAT TAACAATATATTTGTATACAAAACATGCTCCTCAAACATTGAGGTATTATTACAGTACTT AGGTATGAACTTCCAGTCTAATACTGGCCGCAAAAGCCACCTCTCATTACCCAGGATGTA TACAAAAGGCGGATGTGTCAATGGTATTTACAGAAATGTTCCCCAGGGGTATCAAATGGC AAACCCCTTATGTGGCATCTGCTGGAACTTAAGCACCATTTTAAAAAGAAGGAATGACTT TCTGTTGTTTGGAAACTTGGAACACACAAGGTGACTTCAACAGCCCCAGAGGCTCCCAAC CATCACCAACGTTTAGCGCTGTCATGGAGGTGAGGTGGAGGAGGCTGCAAGGCCACCCAG CATCCTGGGATGCAGGCTCTCCAAGGGTCCAGCAGAACCCACACTGGTGGTCCTACTGGC CACCCAGAGCCGGACTCGACAGGGGAACGATTTGAAAGAAACCAACATTCTAAAGGCACA CCATGTTGGTGTAAAAAAATACAAATGTGCAAACGGCAGTGACTGCCACTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005378 |
| Insert Size | 2600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005378.4, NP_005369.2 |
| RefSeq Size | 2613 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 4613 |
| UniProt ID | P04198 |
| Domains | HLH, Myc_N_term |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) lacks segment 1b in the 5' region, compared to variant 1. This variant includes two open reading frames; the isoform 1 represented by this RefSeq is translated from the downstream open reading frame. This transcript and variant 1 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The PLAGL2/MYCN/miR-506-3p interplay regulates neuroblastoma cell fate and associates with neuroblastoma progression
,Zhao, Z;Shelton, SD;Oviedo, A;Baker, AL;Bryant, CP;Omidvarnia, S;Du, L;,
J. Exp. Clin. Cancer Res.
,PubMed ID 32087738
[MYCN]
|
|
Identification of GPC2 as an oncoprotein and candidate immunotherapeutic target in high-risk neuroblastoma
,null,
Cancer cell
,PubMed ID 28898695
[MYCN]
|
|
A combined gene expression and functional study reveals the crosstalk between N-Myc and differentiation-inducing microRNAs in neuroblastoma cells
,Zhao, Z;Ma, X;Shelton, SD;Sung, DC;Li, M;Hernandez, D;Zhang, M;Losiewicz, MD;Chen, Y;Pertsemlidis, A;Yu, X;Liu, Y;Du, L;,
Oncotarget
,PubMed ID 27764804
[MYCN]
|
|
Polyamine Metabolism is Sensitive to Glycolysis Inhibition in Human Neuroblastoma Cells
,Ruiz-Pérez, MV;Medina, MÁ;Urdiales, JL;Keinänen, TA;Sánchez-Jiménez, F;,
J. Biol. Chem.
,PubMed ID 25593318
[MYCN]
|
|
Inhibition of autophagy potentiates the efficacy of Gli inhibitor GANT-61 in MYCN-amplified neuroblastoma cells
,Wang, J;Gu, S;Huang, J;Chen, S;Zhang, Z;Xu, M;,
BMC Cancer
,PubMed ID 25323222
[MYCN]
|
|
Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells
,Hideki Izumi and Yasuhiko Kaneko,
PNAS, Oct 2012; 109: 18048 - 18053.
[MYCN]
|
|
N-myc is a novel regulator of PI3K-mediated VEGF expression in neuroblastoma
,J Kang, P G Rychahou, T A Ishola, J M Mourot, B M Evers, D H Chung,
Oncogene 27, 3999 - 4007 (18 Feb 2008)
[MYCN]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201241 | MYCN (Myc-DDK-tagged)-Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MycN) |
CNY 5,488.00 |
|
| RC201241L1 | Lenti ORF clone of Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201241L2 | Lenti ORF clone of Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), mGFP tagged |
CNY 7,888.00 |
|
| RC201241L3 | Lenti ORF clone of Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201241L4 | Lenti ORF clone of Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), mGFP tagged |
CNY 7,888.00 |
|
| RG201241 | MYCN (tGFP-tagged) - Human v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN) |
CNY 7,088.00 |
