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SMAD4 (NM_005359) Human Untagged Clone
CAT＃: SC116771
SMAD4 (untagged)-Human SMAD family member 4 (SMAD4)
CNY 4,120.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DPC4; JIP; MADH4; MYHRS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116771 sequence for NM_005359 edited (data generated by NextGen Sequencing)
ATGGACAATATGTCTATTACGAATACACCAACAAGTAATGATGCCTGTCTGAGCATTGTG CATAGTTTGATGTGCCATAGACAAGGTGGAGAGAGTGAAACATTTGCAAAAAGAGCAATT GAAAGTTTGGTAAAGAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCT ATAACTACAAATGGAGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGG AGGCTTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTCTGGAGG TGGCCTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTGTCAGTATGCGTTTGAC TTAAAATGTGATAGTGTCTGTGTGAATCCATATCACTACGAACGAGTTGTATCACCTGGA ATTGATCTCTCAGGATTAACACTGCAGAGTAATGCTCCATCAAGTATGATGGTGAAGGAT GAATATGTGCATGACTTTGAGGGACAGCCATCGTTGTCCACTGAAGGACATTCAATTCAA ACCATCCAGCATCCACCAAGTAATCGTGCATCGACAGAGACATACAGCACCCCAGCTCTG TTAGCCCCATCTGAGTCTAATGCTACCAGCACTGCCAACTTTCCCAACATTCCTGTGGCT TCCACAAGTCAGCCTGCCAGTATACTGGGGGGCAGCCATAGTGAAGGACTGTTGCAGATA GCATCAGGGCCTCAGCCAGGACAGCAGCAGAATGGATTTACTGGTCAGCCAGCTACTTAC CATCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTG CCTCACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCCGGACAT TACTGGCCTGTTCACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCT GAGTATTGGTGTTCCATTGCTTACTTTGAAATGGATGTTCAGGTAGGAGAGACATTTAAG GTTCCTTCAAGCTGCCCTATTGTTACTGTTGATGGATACGTGGACCCTTCTGGAGGAGAT CGCTTTTGTTTGGGTCAACTCTCCAATGTCCACAGGACAGAAGCCATTGAGAGAGCAAGG TTGCACATAGGCAAAGGTGTGCAGTTGGAATGTAAAGGTGAAGGTGATGTTTGGGTCAGG TGCCTTAGTGACCACGCGGTCTTTGTACAGAGTTACTACTTAGACAGAGAAGCTGGGCGT GCACCTGGAGATGCTGTTCATAAGATCTACCCAAGTGCATATATAAAGGTCTTTGATTTG CGTCAGTGTCATCGACAGATGCAGCAGCAGGCGGCTACTGCACAAGCTGCAGCAGCTGCC CAGGCAGCAGCCGTGGCAGGAAACATCCCTGGCCCAGGATCAGTAGGTGGAATAGCTCCA GCTATCAGTCTGTCAGCTGCTGCTGGAATTGGTGTTGATGACCTTCGTCGCTTATGCATA CTCAGGATGAGTTTTGTGAAAGGCTGGGGACCGGATTACCCAAGACAGAGCATCAAAGAA ACACCTTGCTGGATTGAAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTT CATACCATGCCGATTGCAGACCCACAACCTTTAGACTGA Clone variation with respect to NM_005359.5 >OriGene 5' read for NM_005359 unedited
GTTCGCATTTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACGAGGCCTCGTGCCG AATTCGGCACGAGGGCTCTCCGGGCCGCCGGGNGAAAGCTACGGGCCCGGTGCGTCCGCG GACCAGCAGCGCGGGAGAGCGGACTCCCCTCGCCACCGCCCGAGCCCAGGTTATCCTGAA TACATGTCTAACAATTTTCCTTGCAACGTTAGCTGTTGTTTTTCACTGTTTCCAAAGGAT CAAAATTGCTTCAGAAATTGGAGACATATTTGATTTAAAAGGAAAAACTTGAACAAATGG ACAATATGTCTATTACGAATACACCAACAAGTAATGATGCCTGTCTGAGCATTGTGCATA GTTTGATGTGCCATAGACAAGGTGGAGAGAGTGAAACATTTGCAAAAAGAGCAATTGAAA GTTTGGTAAAGAAGCTGAAGGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAA CTACAAATGGAGCTCATCCTAGTAAATGTGTTACCATACAGAGAACATTGGATGGGAGGC TTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGATCTATGCCCGTCTCTGGAGGTGGC CTGATCTTCACAAAAATGAACTAAAACATGTTAAATATTGTCAGTATGCGTTTGACTTAN AATGTGATAGTGTCTGTGTGAATCCATATCACTACGAACGAGTTGTATCACCTGGAANTG ATCTCTCAGGATTAACACTGCAGAGTAATGCTCCCATCAGTATGATGGTGAAGGATGAAT ATGTGCATGACTTTGAGGGACAGCCCATCGTTGTCCACTGAGGGACATCANTNCANACCT CCAGCATCCACCCAGTATCGTGCATCGACAGAGACATACAGCACCCCAGCTCTGTTAGCC CCATCTGAGTCTAATGCCTACAGCACTGGCCACTTT >OriGene 3' read for NM_005359 unedited
ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTGAGATTCAG TTTTATTTATGCTACCAGCTAGGTAAAAAGCATACATATTGTGTCTGTGGTACAGTCAAT ACATCTGCAACTTTTCTACTTAAATTCTCTATACAAAGTCACTGCCAATTGATATGATCA TTGATGGCAAAGGGCTTAGAATAACCAAAAGATATAAAAAAGTTTGCAGTCTTGCCCCAA GATACAAAAACTGAATTTTAAACAATATCATAACATACATGATTTAAACATTATACCAAA AAAAGTCACATCAAATCAAGTACAAAAATATCCAAACTACCTATTATGAACTGTAATGTT TCCATTATTCTGCACAGTATTTAACATAGAATTTAGCAGTTTCCAAAATATTCAGTTTAT TCATTATTTAGTTTAAGCATTGCCACCTTTGCAGAACAGTGAGACATTAGGTAGAGTGAC GCTGCTTTTAGTGTTTTAAAAAATACAAAACCTTTCCCTATGAATGAAGGAAAAGAAATT CCTCTTATAGGCTTCTTAGCAACCAACCTTGTGCCTAGAAATACAGAAGAATACAACTAC TGTTTAACAGGGGAAAAGTATACTTCATCAACAGTCATCCACTAGGAATAACAAACGTAA TTTTAAATTNTTTTGTCTGGTAATTNTAAGGGATTCTCAATATACACACAGATTAAGAGT TTTTTGCCTTGGNCAAATAAAACTGAAGTTGCAAGACAATCTGAATTGAAACCATCTTCA CTAATATAACTTGCAAAAAATATTTTGATGAAAGATTACATACATTATTAACTTACTTTT TAAACACAGAGTAAAGTTACTCTTTGGTAAAATNAACTNACCCACATGGCACATTATTTT TGAAGTATGNTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005359 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005359.3, NP_005350.1 |
| RefSeq Size | 3220 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 4089 |
| UniProt ID | Q13485 |
| Domains | DWB, DWA, MH1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway |
| Gene Summary | This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Smad3 and NFAT cooperate to induce Foxp3 expression through its enhancer
,Yukiko Tone, Keiji Furuuchi, Yoshitsugu Kojima, Mark L Tykocinski, Mark I Greene, Masahide Tone,
Nature Immunology 9, 194 - 202 (01 Feb 2008)
[SMAD4]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201828 | SMAD4 (Myc-DDK-tagged)-Human SMAD family member 4 (SMAD4) |
CNY 4,112.00 |
|
| RC201828L1 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), Myc-DDK-tagged |
CNY 6,512.00 |
|
| RC201828L2 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), mGFP tagged |
CNY 6,460.00 |
|
| RC201828L3 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), Myc-DDK-tagged |
CNY 6,512.00 |
|
| RC201828L4 | Lenti ORF clone of Human SMAD family member 4 (SMAD4), mGFP tagged |
CNY 6,512.00 |
|
| RG201828 | SMAD4 (tGFP-tagged) - Human SMAD family member 4 (SMAD4) |
CNY 5,712.00 |
