BCAT1 (NM_005504) Human Untagged Clone

CAT#: SC116691

BCAT1 (untagged)-Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1



  "NM_005504" in other vectors (6)

CNY 3,656.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BCATC; BCT1; ECA39; MECA39; PNAS121; PP18
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116691 sequence for NM_005504 edited (data generated by NextGen Sequencing)
ATGAAGGATTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAG
GTGGTGGGGACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAA
AAACCAGACCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTG
GAGTGGTCCTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCA
TTGCACCCTGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCA
TTTCGAGGAGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATG
TATCGCTCTGCTGTGAGGGCAACTCTGCCGGTATTTGACAAAGAAGAGCTCTTAGAGTGT
ATTCAACAGCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTG
TATATTCGTCCTACATTCATTGGAACTGAGCCTTCTCTTGGAGTCAAGAAGCCTACCAAA
GCCCTGCTCTTTGTACTCTTGAGCCCAGTGGGACCTTATTTTTCAAGTGGAACCTTTAAT
CCAGTGTCCCTGTGGGCCAATCCCAAGTATGTAAGAGCCTGGAAAGGTGGAACTGGGGAC
TGCAAGATGGGAGGGAATTACGGCTCATCTCTTTTTGCCCAATGTGAAGCAGTAGATAAT
GGGTGTCAGCAGGTCCTGTGGCTCTATGGAGAGGACCATCAGATCACTGAAGTGGGAACT
ATGAATCTTTTTCTTTACTGGATAAATGAAGATGGAGAAGAAGAACTGGCAACTCCTCCA
CTAGATGGCATCATTCTTCCAGGAGTGACAAGGCGGTGCATTCTGGACCTGGCACATCAG
TGGGGTGAATTTAAGGTGTCAGAGAGATACCTCACCATGGATGACTTGACAACAGCCCTG
GAGGGGAACAGAGTGAGAGAGATGTTTGGCTCTGGTACAGCCTGTGTTGTTTGCCCAGTT
TCTGATATACTGTACAAAGGCGAGACAATACACATTCCAACTATGGAGAATGGTCCTAAG
CTGGCAAGCCGCATCTTGAGCAAATTAACTGATATCCAGTATGGAAGAGAAGAGAGCGAC
TGGACAATTGTGCTATCCTGA

Clone variation with respect to NM_005504.6
>OriGene 5' read for NM_005504 unedited
CGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
TATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCG
GCACGAGGGGACCGCGCTCTGCAGCCGCAGACCCGGTCCACACGGCCAGGGGCTACGACC
CTTGGGATCTGCCCTCCGCTCAGCTCGAGCTTCCCTCGTGGCCGACGGAACAATGAAGGA
TTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAGGTGGTGGG
GACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAAAAACCAGA
CCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTGGAGTGGTC
CTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCATTGCACCC
TGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCATTTCGAGG
AGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATGTATCGCTC
TGCTGTGAGGGCAACTCTGCCGGTATTTGACANAGAAGAGCTCTTAGAGTGTATTCAACA
GCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTGTATATTCG
TCCTACATTCATTGGAACTGAGCCTTCTCTTGGNAGTCAGAGCCTACCCAGCCCTGCTCT
TTGTACTCTTGAGCCCAGTGGACCTTATTTTTNCAGTGGACCTTNATNCAGTGTCCCTGT
GGCCATCCNAGTATGTAGAGCCTGGAAAGGTGNACTGNNGACTGCAGATGGGNAGGNAAT
ACCGCTCATCTCTTTTGCCCATGTGAGCAGTAATNAATGGTGGTCACCAGGTCCTGTGGC
TTTATGGAGAGGACATCAGATCACTGAGTGGAACTA
>OriGene 3' read for NM_005504 unedited
GTCTACTATGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTCTTAC
ATTTTTTCTTACAACAATCTTTTGAGAAAAGAAAGAGAATGTTTACCAGGTAGAAAAAAC
CTGATTCCTTAACAAGACTTCTCAGTCACTTGTTTCTCTTTCCCTTTCACTTTTCCTTTG
TGCTTTTGAAAACAAAGGCTAAAGCAGGTGTTATAAATTTATTCATTCCTGTGCTAGAGA
GCATGGAACACATCTCTGAAGTCAACTCTTCTTTTGGTGTATCACGAGTTGAATCCTTAA
TAAATGACTGCCACAAAGAAAATTTCTAACAGCAAATCTTTTTAGGAACTGCTCAGGTTT
TGCCTTCGTCTTGACACTTCATTTTAAAATCTTGGTTTCTACTGATGTGTTTTCAAAAAA
GCTGACAAACTCGGTCTGAAATGTAGACACTGAAAAAAGTGAAAGGAAGAGAACATGCAA
TATAAGTTAATCATTATCATTTTAAGTGCCACCAGTTCTGTTTATATTAATGCACTTGTG
AATTGCTGAATCTCATTTGATCAACCCCATTAAACATTAAATAATTGCAGCCAATTATGC
CAATATCAGCCACAATATTAATTTGTTGATCTTTTTGAGCAGTTAGCTTCACATGTTCCT
AAAGCTAGACCATCTTATGCCTTTAAACGATCAATTAGTGTGACAATAACCTAATCCTTG
AAGAATGGATAATTGCCTAGTTATAATGTTGGCACTCCTGCTGTATTAAACCACTGGCAT
TAAACCAATAGTGCACACAGAAAAAAAAAGGGCCAAAGTTCCCTGTTAAATCTTTTTAAC
AAGAAAACGTCCAGGGCAATTTCCGGGGCTATTACCTAAAGGACAAGGTGGGATTGGGGT
CTCCTTTTAAGGAGAACCTCCCGTTTTAAAACACTCTTC
Restriction Sites NotI-NotI     
ACCN NM_005504
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005504.4, NP_005495.2
RefSeq Size 8191 bp
RefSeq ORF 1161 bp
Locus ID 586
UniProt ID P54687
Domains aminotran_4
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation
Gene Summary This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...