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AKAP8 (NM_005858) Human Untagged Clone
CAT＃: SC116499
AKAP8 (untagged)-Human A kinase (PRKA) anchor protein 8 (AKAP8)
CNY 7,616.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AKAP-8; AKAP-95; AKAP 95; AKAP95 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116499 sequence for NM_005858 edited (data generated by NextGen Sequencing)
ATGGACCAGGGCTACGGAGGCTACGGGGCGTGGAGTGCTGGACCTGCCAACACCCAGGGT GCATATGGAACTGGTGTGGCCAGCTGGCAAGGTTATGAAAACTACAATTACTATGGCGCC CAGAACACCAGTGTCACCACAGGCGCAACCTACAGCTACGGCCCAGCCTCGTGGGAGGCC GCCAAGGCCAATGATGGCGGCCTGGCGGCCGGGGCCCCTGCCATGCACATGGCCTCTTAC GGCCCAGAGCCATGCACCGACAATTCCGACTCCCTCATTGCCAAGATCAACCAGCGTTTG GACATGATGTCCAAGGAAGGAGGCAGGGGCGGGAGCGGCGGCGGTGGGGAGGGCATACAG GACCGGGAGAGCTCCTTCCGCTTCCAGCCGTTCGAGTCCTATGACTCCAGGCCCTGCCTG CCGGAGCACAACCCCTACCGCCCCAGCTACAGCTACGACTATGAGTTCGACCTGGGGTCC GACCGCAATGGCAGCTTTGGGGGGCAGTACAGTGAATGCCGAGACCCAGCCCGGGAGCGG GGCTCCCTTGATGGCTTCATGCGGGGCCGGGGCCAGGGCCGCTTCCAGGACCGGAGCAAC CCTGGCACCTTCATGCGCAGCGACCCCTTCGTGCCCCCCGCTGCGTCCTCTGAGCCCCTG TCCACGCCCTGGAACGAGCTGAACTACGTGGGTGGACGGGGCCTGGGAGGGCCCTCCCCC AGCCGGCCACCTCCGTCCCTCTTCTCCCAGTCCATGGCTCCCGACTACGGCGTGATGGGC ATGCAGGGGGCGGGCGGCTATGACAGCACCATGCCCTACGGATGTGGCCGCTCGCAGCCT CGGATGCGGGATCGGGATCGGCCCAAGAGGAGAGGGTTTGACCGCTTCGGACCAGATGGC ACGGGCAGGAAACGGAAGCAGTTCCAACTTTACGAGGAGCCAGACACCAAACTGGCCCGG GTTGACAGTGAAGGAGATTTCTCCGAAAATGATGACGCAGCTGGTGACTTCCGCTCAGGA GATGAAGAATTCAAGGGTGAGGATGAACTCTGCGACTCTGGGAGGCAAAGAGGAGAGAAG GAGGACGAGGACGAGGATGTGAAGAAGAGAAGGGAAAAGCAAAGGAGAAGAGACAGGACG CGGGACCGTGCAGCCGACAGAATTCAGTTTGCCTGTTCTGTATGCAAGTTCCGTAGCTTT GATGACGAAGAGATCCAGAAGCATCTGCAAAGCAAATTTCACAAAGAGACCCTGCGGTTC ATAAGCACCAAGCTGCCCGACAAGACCGTGGAGTTCCTCCAGGAATACATTGTAAACAGA AATAAGAAAATTGAGAAGCGGCGTCAGGAATTGATGGAGAAAGAAACCGCAAAACCAAAA CCAGATCCTTTCAAAGGGATTGGCCAGGAGCACTTCTTCAAGAAGATCGAGGCTGCTCAC TGCCTGGCCTGCGACATGCTAATTCCTGCACAGCCGCAGCTCCTCCAGCGGCACCTGCAC TCCGTGGACCACAATCACAACCGCAGGTTGGCTGCTGAACAGTTCAAGAAAACCAGTCTC CATGTGGCTAAGAGTGTTTTGAACAACAGACATATAGTGAAGATGCTGGAAAAATACCTC AAGGGTGAGGACCCTTTCACCAGTGAAACTGTTGATCCAGAAATGGAAGGAGATGACAAT TTAGGAGGTGAGGATAAGAAAGAGACACCTGAGGAGGTGGCCGCGGACGTCTTAGCAGAG GTGATTACAGCAGCAGTGAGGGCCGTAGATGGGGAAGGAGCGCCCGCTCCAGAGAGCAGC GGGGAGCCGGCTGAGGACGAAGGCCCCACGGACACAGCGGAGGCCGGTAGTGATCCTCAA GCCGAACAGCTGCTGGAAGAGCAGGTGCCCTGTGGAACGGCACATGAGAAGGGCGTCCCC AAGGCCAGAAGTGAGGCTGCAGAGGCTGGAAATGGCGCCGAGACAATGGCAGCAGAGGCA GAAAGTGCCCAAACCAGAGTTGCTCCTGCCCCAGCTGCCGCGGATGCTGAAGTGGAACAA ACTGATGCAGAGTCTAAAGACGCTGTTCCCACAGAATGA Clone variation with respect to NM_005858.3 >OriGene 5' read for NM_005858 unedited
GGGTGCACAATATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGTGC GCTGAACGCATGCGTGCTGTGGTCGCCTAGTAAACGGGGCTGCTGGTGGGCCGCGTCGAA GACATGGACCAGGGCTACGGAGGCTACGGGGCGTGGAGTGCTGGACCTGCCAACACCCAG GGTGCATATGGAACTGGTGTGGCCAGCTGGCAAGGTTATGAAAACTACAATTACTATGGC GCCCAGAACACCAGTGTCACCACAGGCGCAACCTACAGCTACGGCCCAGCCTCGTGGGAG GCCGCCAAGGCCAATGATGGCGGCCTGGCGGCCGGGGCCCCTGCCATGCACATGGCCTCT TACGGCCCAGAGCCATGCACCGACAATTCCGACTCCCTCATTGCCAAGATCAACCAGCGT TTGGACATGATGTCCAAGGAAGGAGGCAGGGGCGGGAGCGGCGGCGGTGGGGAGGGCATA CAGGACCGGGAGAGCTCCTTCCGCTTCCAGCCGTTCGAGTCCTATGACTCCAGGCCCTGC CTGCCGGAGCACAACCCCTACCGCCCCAGCTACAGCTACGACTATGAGTTCGACCTGNGG TCCGACCGCAATGGCAGCTTTGGGGGGCAGTACAGTGAATGCCGAGACCCAGCCCGGGAG CGGNGCTCCCTTGATGGCTTCATGCGGNGCCGGGGCCAGGGCCGCTTCCCAGACCGGAGC AACCCTGGCACCTTCATGCGCAGCGACCCCCTTCGTGCCCCCCGCTGCGTCCTCTGAGCC CCTGTNCACGCCCTGNAACGAGCTGAACTACCGTGGGTGGACGGNGCCCTGGGAGGGCCC CCCTCCCCGGCCGGCCACCNTCCGTCCCNTCTTCTCCCAGTCCATTGGCTCCCGACTACN GCGTGATGGGCATGCAGGGGGGCGGCCNCTATGACAGCACATGCCCTACGATGG >OriGene 3' read for NM_005858 unedited
CTATGAACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAAAAAAGG GGTCTTGCTATGTTGTCCAGGTTGGTCTTGAACTCCTGGGCTTAAGCTATCCTCTCACCA CGGCCTCCCCAAGTGCTGGGATTATAGGTATGCATCACCGTGCCTGGCCAAATTCTCTTT GTAAATACATTTGAGTGGTAGTAACTAATTTTAAAGGAAAAAAACCTGAAGTAATCTAGA GGTGGAACACAAATGAGGTAAAATATTAGAGAACTGATTCAGCAAGGACTAAAGATTGGG ACGCAATGCCCTCTGCACAGTCTGGTTCTTGAGGTTGCGGTGGGTGTGTTTCCAGTGGCG TAGGTCGGTCAGATACTTCTGGCCTTTGGGAGTGCGTGGATGTGACGCGGTCAGTGCCAC ATGTGAACCAGGTGCTGCGAAGTCCTGTGAAAGTCACACACCATATGGTGCACTACAAAG GTATAGGTTAAAGGCTGAAGCTTGAAACATACAAAAAAAAATCCTCTATAGAGCAAGATA TATTCCTAGTATTCAGCTGTTCCCAACCAAATAAAAAAAAAAAATTAAGGAAAAAAATAA AAAAGGAAGCATTCCATCAGTGGCTGTATTGAACAAGTAATGTATCATCAAGATATAATC ACCCAACCTTTATTATTCCAAGTGCACTACTGTCAAGAGACATGTTACAGCCGCTAGGGA CAATGTACTCAAGCTTTGAAACCTGGGCAAGAAACCCACGACTGCATGAACAAGCCGTGA GAGCACTGTTCAGGAGGAAACCTGGCCTCTTCCCCAAATAGATTCAGCTTATTTGGAGCA CCCCCTTGACTGGTTACAACCACGGAAAAGCACACCCCTTCTTATTCCAAAGCCTTCTGG GACAGGGATATACAATATTTGTGCCACCCGCTTTCACACCGGCACATTTGTCGCTTAACC TGCGGGCATTGGGCCGAACACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005858 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005858.2, NP_005849.1 |
| RefSeq Size | 3545 bp |
| RefSeq ORF | 2079 bp |
| Locus ID | 10270 |
| UniProt ID | O43823 |
| Domains | AKAP95 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205349 | AKAP8 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 8 (AKAP8) |
CNY 7,600.00 |
|
| RC205349L1 | Lenti-ORF clone of AKAP8 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 8 (AKAP8) |
CNY 10,000.00 |
|
| RC205349L2 | Lenti-ORF clone of AKAP8 (mGFP-tagged)-Human A kinase (PRKA) anchor protein 8 (AKAP8) |
CNY 5,890.00 |
|
| RC205349L3 | Lenti-ORF clone of AKAP8 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 8 (AKAP8) |
CNY 5,890.00 |
|
| RC205349L4 | Lenti-ORF clone of AKAP8 (mGFP-tagged)-Human A kinase (PRKA) anchor protein 8 (AKAP8) |
CNY 5,890.00 |
|
| RG205349 | AKAP8 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 8 (AKAP8) |
CNY 9,200.00 |
