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MDH1 (NM_005917) Human Untagged Clone
CAT＃: SC116447
MDH1 (untagged)-Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE88; EIEE88; HEL-S-32; KAR; MDH-s; MDHA; MGC:1375; MOR2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116447 sequence for NM_005917 edited (data generated by NextGen Sequencing)
ATGTCTGAACCAATCAGAGTCCTTGTGACTGGAGCAGCTGGTCAAATTGCATATTCACTG CTGTACAGTATTGGAAATGGATCTGTCTTTGGTAAAGATCAGCCTATAATTCTTGTGCTG TTGGATATCACCCCCATGATGGGTGTCCTGGACGGTGTCCTAATGGAACTGCAAGACTGT GCCCTTCCCCTCCTGAAAGATGTCATCGCAACAGATAAAGAAGACGTTGCCTTCAAAGAC CTGGATGTGGCCATTCTTGTGGGCTCCATGCCAAGAAGGGAAGGCATGGAGAGAAAAGAT TTACTGAAAGCAAATGTGAAAATCTTCAAATCCCAGGGTGCAGCCTTAGATAAATACGCC AAGAAGTCAGTTAAGGTTATTGTTGTGGGTAATCCAGCCAATACCAACTGCCTGACTGCT TCCAAGTCAGCTCCATCCATCCCCAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCAC AACCGAGCTAAAGCTCAAATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAAT GTCATTATCTGGGGAAACCATTCCTCGACTCAGTATCCAGATGTCAACCATGCCAAGGTG AAATTGCAAGGAAAGGAAGTTGGTGTTTATGAAGCTCTGAAAGATGACAGCTGGCTCAAG GGAGAATTTGTCACGACTGTGCAGCAGCGTGGCGCTGCTGTCATCAAGGCTCGAAAACTA TCCAGTGCCATGTCTGCTGCAAAAGCCATCTGTGACCACGTCAGGGACATCTGGTTTGGA ACCCCAGAGGGAGAGTTTGTGTCCATGGGTGTTATCTCTGATGGCAACTCCTATGGTGTT CCTGATGATCTGCTCTACTCATTCCCTGTTGTAATCAAGAATAAGACCTGGAAGTTTGTT GAAGGTCTCCCTATTAATGATTTCTCACGTGAGAAGATGGATCTTACTGCAAAGGAACTG ACAGAAGAAAAAGAAAGTGCTTTTGAATTTCTTTCCTCTGCCTGA Clone variation with respect to NM_005917.3 >OriGene 5' read for NM_005917 unedited
TACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCGGTAGAGGTGACCTGACTCTCT GAGGCTCATTTTGCAGTTGTTGAAATTGTCCCCGCAGTTTTCAATCATGTCTGAACCAAT CAGAGTCCTTGTGACTGGAGCAGCTGGTCAAATTGCATATTCACTGCTGTACAGTATTGG AAATGGATCTGTCTTTGGTAAAGATCAGCCTATAATTCTTGTGCTGTTGGATATCACCCC CATGATGGGTGTCCTGGACGGTGTCCTAATGGAACTGCAAGACTGTGCCCTTCCCCTCCT GAAAGATGTCATCGCAACAGATAAAGAAGACGTTGCCTTCAAAGACCTGGATGTGGCCAT TCTTGTGGGCTCCATGCCAAGAAGGGAAGGCATGGAGAGAAAAGATTTACTGAAAGCAAA TGTGAAAATCTTCAAATCCCAGGGTGCAGCCTTAGATAAATACGCCAAGAAGTCAGTTAA GGTTATTGTTGTGGGTAATCCAGCCAATACCAACTGCCTGACTGCTTCCAAGTCAGCTCC ATCCATCCCCAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCACAACCGAGCTAAAGC TCANATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAATGTCATTATCTGGNG AAACCATTCCTCGACTCAGTATCCAGATGTCAACCATGCCAAGGGTGAAATTGCAAGGAA AGGAAGNNTGGTGTTTATGAAAGCTCTGAAAGATGACAGCTGGCTCAAGGGAGATTTGTC ACGACTGTGCAGCAGCGTGGCGCTTCTGTCATCAAGGCTCGAAACTATCCATGCCATGTC TGCTGCAAAGCCATCTGTGACACGTCAGGGACATCTGGTTNGGGACCCAAGGAAGAGTTT GGTCATGGGTGTATCTCTGAGGCACTCCTAGGNGTCCTGAGAACTGCCTACTCATCCTGT GTATCAGATAGACCTGGAGTTGGTG >OriGene 3' read for NM_005917 unedited
GCAACTTTCCAGGGCCAGGAATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT TTCACTTTCATTTGAATATATATTTATTTAGAATGCACACTAACAGCATGACGATAAACT GTCATTCACAAACCTGTACCAAGAAGCACGTAATCTTTAAAATGTGTTGTTTTTCACAAG TAATTTAAGTATAGCATTATTATTATTTGGTACTTGAGTCAAAGACGACATTTAGATTCT TCAGCTTTGAAGCATTTAGTAACATCATTGTCTAGTCAGGCAGAGGAAAGAAATTCAAAA GCACTTTCTTTTTCTTCTGTCAGTTCCTTTGCAGTAAGATCCATCTTCTCACGTGAGAAA TCATTAATAGGGAGACCTTCAACAAACTTCCAGGTCTTATTCTTGATTACAACAGGGAAT GAGTAGAGCAGATCATCAGGAACACCATAGGAGTTGCCATCAGAGATAACACCCATGGAC ACAAACTCTCCCTCTGGGGTTCCAAACCAGATGTCCCTGACGTGGTCACAGATGGCTTTT GCAGCAGACATGGCACTGGATAGTTTTCGAGCCTTGATGACAGCAGCGCCACGCTGCTGC ACAGTCGTGACAAATTCTCCCTTGAGCCAGCTGTCATCTTTCAGAGCTTCATAACACCAA CTTCCTTTCCTTGCATTTTCACCTTGGCATGGTTGACATCTGGATACTGAGTCGAGAAAT GGTTTCCCCAGATATGACATTCTTTACATCATAACAGNCACACAAGTTTAGAGCTTTTGA GCTTACCTCGGTTGGGATCCAACGAGTCAGCAACTGAGTTCTCCTTGGGGATGAAGGAGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005917 |
| Insert Size | 1370 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005917.2, NP_005908.1 |
| RefSeq Size | 1268 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 4190 |
| UniProt ID | P40925 |
| Domains | ldh |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism |
| Gene Summary | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MDH1), which is localized in the cytosol. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ribosomal Readthrough at a Short UGA Stop Codon Context Triggers Dual Localization of Metabolic Enzymes in Fungi and Animals
,Stiebler, AC;Freitag, J;Schink, KO;Stehlik, T;Tillmann, BA;Ast, J;Bölker, M;,
PLoS Genet.
,PubMed ID 25340584
[MDH1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200298 | MDH1 (Myc-DDK-tagged)-Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2 |
CNY 3,656.00 |
|
| RC200298L1 | Lenti ORF clone of Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200298L2 | Lenti ORF clone of Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200298L3 | Lenti ORF clone of Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200298L4 | Lenti ORF clone of Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG200298 | MDH1 (tGFP-tagged) - Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2 |
CNY 5,256.00 |
|
| SC320854 | MDH1 (untagged)-Human malate dehydrogenase 1, NAD (soluble) (MDH1), transcript variant 2 |
CNY 3,656.00 |
