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MADH7 (SMAD7) (NM_005904) Human Untagged Clone
CAT＃: SC116437
SMAD7 (untagged)-Human SMAD family member 7 (SMAD7), transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRCS3; MADH7; MADH8 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116437 sequence for NM_005904 edited (data generated by NextGen Sequencing)
ATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGC GGCGAGGACGAGGAGGAGGGCGCAGGGGGAGGTGGAGGAGGAGGCGAGCTGCGGGGAGAA GGGGCGACGGACAGCCGAGCGCATGGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGC TGCCTGGGCAAGGCGGTGCGAGGTGCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCG GGCGCCGGCGCGGCCGGGGGCGCCGAGGCGGATCTGAAGGCGCTCACGCACTCGGTGCTC AAGAAACTGAAGGAGCGGCAGCTGGAGCTGCTGCTCCAGGCCGTGGAGTCCCGCGGCGGG ACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCCTGGACTGCAGGCTGGGCCCGGGGGCG CCCGCCGGCGCGCAGCCTGCGCAGCCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGC AAAGTGTTCAGGTGGCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGT GAATCTTACGGGAAGATCAACCCCGAGCTGGTGTGCTGCAACCCCCATCACCTTAGCCGA CTCTGCGAACTAGAGTCTCCCCCCCCTCCTTACTCCAGATACCCGATGGATTTTCTCAAA CCAACTGCAGACTGTCCAGATGCTGTGCCTTCCTCCGCTGAAACAGGGGGAACGAATTAT CTGGCCCCTGGGGGGCTTTCAGATTCCCAACTTCTTCTGGAGCCTGGGGATCGGTCACAC TGGTGCGTGGTGGCATACTGGGAGGAGAAGACGAGAGTGGGGAGGCTCTACTGTGTCCAG GAGCCCTCTCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTCGGACAG CTCAATTCGGACAACAAGAGTCAGCTGGTGCAGAAGGTGCGGAGCAAAATCGGCTGCGGC ATCCAGCTGACGCGGGAGGTGGATGGTGTGTGGGTGTACAACCGCAGCAGTTACCCCATC TTCATCAAGTCCGCCACACTGGACAACCCGGACTCCAGGACGCTGTTGGTACACAAGGTG TTCCCCGGTTTCTCCATCAAGGCTTTCGACTACGAGAAGGCGTACAGCCTGCAGCGGCCC AATGACCACGAGTTTATGCAGCAGCCGTGGACGGGCTTTACCGTGCAGATCAGCTTTGTG AAGGGCTGGGGCCAGTGCTACACCCGCCAGTTCATCAGCAGCTGCCCGTGCTGGCTAGAG GTCATCTTCAACAGCCGGTAG Clone variation with respect to NM_005904.3 >OriGene 5' read for NM_005904 unedited
GCGTAATTCGCAGAGGCGCGCGCCCCGGCCTCCGGGAGACTGGCGCATGCCACGGAGCGC CCCTCGGGCCGCCGCCGCTCCTGCCCGGGCCCCTGCTGCTGCTGCTGTCGCCTGCGCCTG CTGCCCCAACTCGGCGCCCGACTTCTTCATGGTGTGCGGAGGTCATGTTCGCTCCTTAGC AGGCAAACGACTTTTCTCCTCGCCTCCTCGCCCCGCATGTTCAGGACCAAACGATCTGCG CTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGCGGCGAGGACGAGGAGGAGGGCGCA GGGGGAGGTGGAGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACAGCCGAGCGCAT GGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGCTGCCTGGGCAAGGCGGTGCGAGGT GCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCGGGCGCCGGCGCGGCCGGGGGCGCC GAGGCGGATCTGAAGGCGCTCACGCACTCGGTGCTCAAGAACTGAAGGAGCGGCAGCTGG AGCTGCTGCTCCAGCCGGTGGAGTCCCGCGGCGGGACGCGCACCGCGTGCCTCCTGCTGC CCGGCCGCCTGGACTGCANGCTGGCCCCGGGGGGCGCCCGCCGGCGCGCAGNCTGCGCAG ACGACCCTCGTCCTACTCGCTCCCCCTTCTGCTGTGNAAAGTGGTNCAAGGGGGCCGGAT CTCAGGCATTCTTCGGAAGTCAAAAGGCTGGGNTTGCTGCGAATCCTACCGGGAAGATCA ACCCGAACCCTGTGGTGCTGGACCCCCCTCACCTTAACCCGACTCTGCGAACTAAAATCT CCCCCCCCTCCTTACTTCAGATACCGGGAGTGAATTTCTTAA >OriGene 3' read for NM_005904 unedited
ACTTGTTTGCTTTTAAAAACGCCTTTGGGCCTAAACTCCTTTATAAAACATCAGCTAGGT GATAACACCCATAGAAAAAAACACCAATCTTGTGTTTATCTTTTTTAATTTGGCATTTGT TATTTGCATTTATATTAAAGCAAAGTGCATCTTTTCTTTATTTTTCTTGTTTATACACAT TGCACAATACATAAATAATGATGCTTATAAAACGTCTTTATATTTACAAGTAATAATATA TTTATATATAACATAAAATACATTTTTTTCTTTAATAAATCTCAGGTTTTTTTTCAGGAG TCCTTTCTCTCTCAAAGCACTACAATGCTAAATTTCCAATGAGAATGCTTCTCTTGTTCA TTTAAACCTTTGTAGTTAGAAAAATAGCTTATGTTAAAGTCTAAACATGCTCATTGAGCT AAGAACAGTGTCGAAGTATCATACGAGTGTATGAGTTGTAGAAGAAATGAAAGAAGAGTT AGGTGTCAGCCTAGGATGGTACCTTGGGTTATGACGGACCAAATCCTTCTTGGAGGGAAG GGGACTGCTAAGCATGTCCCTCCCAAGGACATCCCCGCTTGCTGGCCTAATAGCAGAGCT TAACACACAGGATGGGAGCAGGCAGTGCTGAGCCTGGTTTCTAAGTCCGGGAAAAACCTG ATGTCACCAAGGCCAGCCANGGGAAAAGCCTGAAGGGAAAGGCCCACTGGGTTGAGCAAT ACTGTGAAGGGGCTTGCCCCGGGCACCCCTTGGGAAGCCCATTCTTAGGGAGATCCACGA ACCAGATTGGCCAAAAAAAAAAGACCCTCCATTGTCAAGGGCTTGGCAGGAAGGAAATAA AACAAGGGTTGAAATAATTGGTTCTGGGTTGGACACTTTCAAGCCTCCTTGTTCAAAAAC CTGGGAACAGGCCCCGTCCTTGTTGAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005904 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005904.2, NP_005895.1 |
| RefSeq Size | 3103 bp |
| RefSeq ORF | 1281 bp |
| Locus ID | 4092 |
| UniProt ID | O15105 |
| Domains | DWB, DWA, MH1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MicroRNA-92b promotes hepatocellular carcinoma progression by targeting Smad7 and is mediated by long non-coding RNA XIST
,Zhuang, LK;Yang, YT;Ma, X;Han, B;Wang, ZS;Zhao, QY;Wu, LQ;Qu, ZQ;,
Cell Death Dis
,PubMed ID 27100897
[SMAD7]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210400 | SMAD7 (Myc-DDK-tagged)-Human SMAD family member 7 (SMAD7), transcript variant 1 |
CNY 5,488.00 |
|
| RC210400L1 | Lenti ORF clone of Human SMAD family member 7 (SMAD7), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210400L2 | Lenti ORF clone of Human SMAD family member 7 (SMAD7), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC210400L3 | Lenti ORF clone of Human SMAD family member 7 (SMAD7), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210400L4 | Lenti ORF clone of Human SMAD family member 7 (SMAD7), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG210400 | SMAD7 (tGFP-tagged) - Human SMAD family member 7 (SMAD7), transcript variant 1 |
CNY 7,088.00 |
