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Isocitrate dehydrogenase (IDH1) (NM_005896) Human Untagged Clone
CAT＃: SC116430
IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1)
CNY 5,488.00
| Cited in 9 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116430 sequence for NM_005896 edited (data generated by NextGen Sequencing)
ATGTCCAAAAAAATCAGTGGCGGTTCTGTGGTAGAGATGCAAGGAGATGAAATGACACGA ATCATTTGGGAATTGATTAAAGAGAAACTCATTTTTCCCTACGTGGAATTGGATCTACAT AGCTATGATTTAGGCATAGAGAATCGTGATGCCACCAACGACCAAGTCACCAAGGATGCT GCAGAAGCTATAAAGAAGCATAATGTTGGCGTCAAATGTGCCACTATCACTCCTGATGAG AAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAATCACCAAATGGCACCATACGA AATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATTATCTGCAAAAATATCCCCCGGCTT GTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGATCAATACAGA GCAACTGATTTTGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAGTGAC GGAACCCAAAAGGTGACATACCTGGTACATAACTTTGAAGAAGGTGGTGGTGTTGCCATG GGGATGTATAATCAAGATAAGTCAATTGAAGATTTTGCACACAGTTCCTTCCAAATGGCT CTGTCTAAGGGTTGGCCTTTGTATCTGAGCACCAAAAACACTATTCTGAAGAAATATGAT GGGCGTTTTAAAGACATCTTTCAGGAGATATATGACAAGCAGTACAAGTCCCAGTTTGAA GCTCAAAAGATCTGGTATGAGCATAGGCTCATCGACGACATGGTGGCCCAAGCTATGAAA TCAGAGGGAGGCTTCATCTGGGCCTGTAAAAACTATGATGGTGACGTGCAGTCGGACTCT GTGGCCCAAGGGTATGGCTCTCTCGGCATGATGACCAGCGTGCTGGTTTGTCCAGATGGC AAGACAGTAGAAGCAGAGGCTGCCCACGGGACTGTAACCCGTCACTACCGCATGTACCAG AAAGGACAGGAGACGTCCACCAATCCCATTGCTTCCATTTTTGCCTGGACCAGAGGGTTA GCCCACAGAGCAAAGCTTGATAACAATAAAGAGCTTGCCTTCTTTGCAAATGCTTTGGAA GAAGTCTCTATTGAGACAATTGAGGCTGGCTTCATGACCAAGGACTTGGCTGCTTGCATT AAAGGTTTACCCAATGTGCAACGTTCTGACTACTTGAATACATTTGAGTTCATGGATAAA CTTGGAGAAAACTTGAAGATCAAACTAGCTCAGGCCAAACTTTAA Clone variation with respect to NM_005896.2 >OriGene 5' read for NM_005896 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATCCCGGCAGGCGATA AACTACATTCAGTTGAGTCTGCAAGACTGGGAGGAACTGGGGTGATAAGAAATCTATTCA CTGTCAAGGTTTATTGAAGTCAAAATGTCCAAAAAAATCAGTGGCGGTTCTGTGGTAGAG ATGCAAGGAGATGAAATGACACGAATCATTTGGGAATTGATTAAAGAGAAACTCATTTTT CCCTACGTGGAATTGGATCTACATAGCTATGATTTAGGCATAGAGAATCGTGATGCCACC AACGACCAAGTCACCAAGGATGCTGCAGAAGCTATAAAGAAGCATAATGTTGGCGTCAAA TGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGG AAATCACCAAATGGCACCATACGAAATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATT ATCTGCAAAAATATCCCCCGGCTTGTGAGTGGATGGGTAAAACCTATCATCATAGGTCGT CATGCTTATGGGGATCAATACAGAGCAACTGATTTTGTTGTTCCTGNGCCTGGAAAAGTA GAGATAACCTACACACCAAGTGACGGAACCCAAAAGGTGACATACCTGGTACATAACTTT GAAGAAGGGTGGTGGTGTTGCCATGGNNGATGTATAATCAAGATAAGTCAATTGAAGATT TTGCACACAGTTCCTTCCAATGGCTCTGTCTAAGGGTTNGGCCTTTGTATCTGAGCCCAA AACACTATTCTGAGAATATGANTGGGCGTTTAAGACATCTTCAGAGATTATGACAGCAGT ACAGGTCCAGTTGAGCTCANNAGATCTGTATGACATAGCTCATCACN >OriGene 3' read for NM_005896 unedited
CGCAATCTAGAGTCGAGTNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTAAAGGGGAATTACCCCTTATTTTTAATTAAAATCACCCGTTCAGGAAAAAATAAA ATAGGGTTAAATTTCAATTTCTGAAAAATCCCTTTTTTTTAAAAAAAATAAACCGGGCCC CCAAAAGTCCAAAAAAAATTCACCTTACTTTTTTGCCCTTGGATAAAATTTCCTTTTTAA AGTATTTTAATTTTCAGGCCCGCCCCGGAGGAAAAAAAAAAAAATGGGGAGGACAACCCT CCAGGTAGTTTTTTTTCCGGATTCCAAACTTTCCTGGGGCCTAAACAGTTTTTATTCTTT TGGAAACATTCACCAGGGTTTTTACAAAAATGACTGCAGTTTCTTCAACCCATTTGAGTG GCACTCATCCTTCGTTCCAGTCTTGTGCAATTTTAATGCCCACTTCTTCCTTAAAAAACG GATTTTTTTTACCCCAAATTTCCCTGTCCCCAAGTTTTGGCCAGGAGGGGAAGCCCGGAA ATTCCCTTCTTCGCTTTTTAGGGGACATTCCGGGGGTTTTTTTCCCCTTTAGAACCCTTG TTTTTGGCCTTTTTTATTAAACCCGGAAAAAAGGGCCGGGATCCCAGTGTTCCTCCCCGA TTTCTTGGGGGGGCCCAAAAAAAAAAAAAAATTTTAAAAACCCCCTTCCTTTTTCCCAAA GGGGGCGATACGTTTTTTAAAAAAAAGGGGGGGACCTTTAAAAAAAAAAACTTGGGTTTT TAAAAAAAAAAAAAAAAGGGTCCCCTTTTCCCTCGGGGGCAACAACAAAAAGAAACCCTG CCCACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005896 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005896.2, NP_005887.2 |
| RefSeq Size | 2339 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 3417 |
| UniProt ID | O75874 |
| Domains | isodh |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
| Gene Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein. |
Citations (9)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Limited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors
,null,
Nature Cancer
,PubMed ID 35681100
[IDH1]
|
|
RNA-binding Protein HuR Regulates Both Mutant and Wild-type IDH1 in IDH1-mutated Cancer
,Zarei, M;Lal, S;Vaziri-Gohar, A;O'Hayer, K;Gunda, V;Singh, PK;Brody, JR;Winter, JM;,
Mol. Cancer Res.
,PubMed ID 30266754
[IDH1]
|
|
Posttranscriptional Upregulation of IDH1 by HuR Establishes a Powerful Survival Phenotype in Pancreatic Cancer Cells
,null,
Cancer research
,PubMed ID 28652247
[IDH1]
|
|
A Highly Facile and Specific Assay for Cancer-Causing Isocitrate Dehydrogenase Mutant Using (13)C4-Labeled a-Ketoglutarate and Heteronuclear NMR
,Wen, H;Yun, T;Xu, WJ;Choi, SH;Kim, H;Park, CK;Lee, SH;Park, SW;Lee, SK;Park, S;,
Anal. Chem.
,PubMed ID 24266712
[IDH1]
|
|
IDH1 and IDH2 mutations in pediatric acute leukemia
,null,
Leukemia
,PubMed ID 21647154
[IDH1]
|
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IDH1 and IDH2 mutations in pediatric acute leukemia
,A K Andersson, D W Miller, J A Lynch, A S Lemoff, Z Cai, S B Pounds, I Radtke, B Yan, J D Schuetz, J E Rubnitz, et al.,
Leukemia (7 June 2011) doi:10.1038/leu.2011.133 Original Article
[IDH1]
|
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Cancer-associated metabolite 2-hydroxyglutarate accumulates in acute myelogenous leukemia with isocitrate dehydrogenase 1 and 2 mutations
,Stefan Gross, Rob A. Cairns, Mark D. Minden, Edward M. Driggers, Mark A. Bittinger, Hyun Gyung Jang, Masato Sasaki, Shengfang Jin, David P. Schenkein, Shinsan M. Su, Lenny Dang, Valeria R. Fantin, and Tak W. Mak,
J. Exp. Med., Feb 2010; 207: 339 - 344
[IDH1]
|
|
Cancer-associated IDH1 mutations produce 2-hydroxyglutarate
,Lenny Dang, David W. White, Stefan Gross, Bryson D. Bennett, Mark A. Bittinger, Edward M. Driggers, Valeria R. Fantin, Hyun Gyung Jang, Shengfang Jin, Marie C. Keenan, et al,
Nature (22 November 2009)
[IDH1]
|
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IDH1 and IDH2 Mutations in Gliomas
,Hai Yan, D. Williams Parsons, Genglin Jin, Roger McLendon, B. Ahmed Rasheed, Weishi Yuan, Ivan Kos, Ines Batinic-Haberle, Siân Jones, Gregory J. Riggins, Henry Friedman, Allan Friedman, David Reardon, James Herndon, Kenneth W. Kinzler, Victor E. Velculescu, Bert Vogelstein, and Darell D. Bigner,
N. Engl. J. Med., Feb 2009; 360: 765 - 773.
[IDH1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210582 | IDH1 (Myc-DDK-tagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 5,488.00 |
|
| RC210582L1 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210582L2 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), mGFP tagged |
CNY 7,888.00 |
|
| RC210582L3 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210582L4 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), mGFP tagged |
CNY 7,888.00 |
|
| RG210582 | IDH1 (tGFP-tagged) - Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 7,088.00 |
|
| SC322129 | IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 5,488.00 |
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