AKR1D1 (NM_005989) Human Untagged Clone

CAT＃: SC116410

AKR1D1 (untagged)-Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1

Datasheet
SDS

  "NM_005989" in other vectors (6)

CNY 2,400.00

CNY 6,270.00

货期*
现货
规格
    • 10 ug
Cited in 7 publications.

Product images

经常一起买 (4)
TA324084
Anti-AKR1D1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

暂停销售
TF81001
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms 3o5bred; CBAS2; SRD5B1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_005989 edited
ATGGATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATC
ATCGGACTTGGTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCG
GTGAAGGTTGCTATTGACACAGGGTACCGACATATTGATGGGGCCTACATCTACCAAAAT
GAACACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGAAGGAAAGGTGCGGAGGGAA
GATATCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGTCCCAGAGATGGTCCGCCCA
ACCCTGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTACATCATTGAA
GTACCCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGG
TTATATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCT
GGCTTGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAGGCAGCTGGAGCTCATCCTG
AACAAGCCAGGACTCAAACACAAGCCAGTCAGCAACCAGGTTGAGTGCCATCCGTATTTC
ACCCAGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGC
CCTTTGGGGACCAGTAGGAATCCAATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAG
GATGCACTTCTAAACTCATTGGGGAAAAGGTACAATAAGACAGCAGCTCAAATTGTTTTG
CGTTTCAACATCCAGCGAGGGGTGGTTGTCATTCCTAAAAGCTTTAATCTTGAAAGGATC
AAAGAAAATTTTCAGATCTTTGACTTTTCTCTCACTGAAGAAGAAATGAAGGACATTGAA
GCCTTGAATAAAAATGTCCGCTTTGTAGAATTGCTCATGTGGCGCGATCATCCTGAATAC
CCATTTCATGATGAATACTGA
>OriGene 5' read for NM_005989 unedited
NNGGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGCACAATGG
ATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATCATCG
GACTTGGTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCGGTGA
AGGTTGCTATTGACACAGGGTACCGACATATTGATGGGGCCTACATCTACCAAAATGAAC
ACGAAGTTGGGGAGGCCATCAGGGAGAAGATAGCAGAAGGAAAGGTGCGGAGGGAAGATA
TCTTCTACTGTGGAAAGCTATGGGCTACAAATCATGTCCCAGAGATGGTCCGCCCAACCC
TGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTACATCATTGAAGTAC
CCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGGTTAT
ATCACAAGTCAAATCTGTGTGCCACTTGGGAGGCGATGGAAGCTTGCAAAGACGCTGGCT
TGGTGAAATCCCTGGGAGTGTCCAATTTTAACCGCAGGCAGCTGGAGCTCATCCTGAACA
AGCCAGGACTCAAACACAAGCCAGTCAGCAACCAGGTTGAGTGCCATCCGTATTTCACCC
AGCCAAAACTCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGCCCTT
TGGGGACCAGTANGAATCCAATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAGGATG
CACTTCTAAACTCATTGGGGAAAAGGTACAATAAGACAGCAGCTCANATTGTTTTTGCGT
TTCAACATCCAGCGAGGGGGTGGTTGTCATTCCTAAAAGCTTTAATCTTGAAAGGATCAA
AGAAAATTTTCAGATCTTTGACTTTTT
>OriGene 3' read for NM_005989 unedited
TTGACGCATTGGCGATTGGCACCTTTCCAGGTCCAGTACAGTCACTGGGGAATGGGTCAC
AGGGCTGCCCCCCGTGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATATTC
GAGTTTTTTTTTTTTTTTTTTTTTTTTTGATGCAACAAATCATTATACTTTATTATTTCT
TGTGAACAAAATATTTTCCAGAGGTCAGTGTTATAAGTAATAATATTGGAATCAGTGAAA
ACAATCAGTATAGACCAAATTTTTTAAAATGATAATTTCTCAAAAATATATGTCTTGAGG
TCATTGAACATTATCTATAAGCAACTTATCTTGGGAAAACATAATTTTTGAAGAACTCTC
TCATCCTATGACAGTATGACAGTGACCTAATGTGAATGAATAAAATATATTTCTTTTCTT
TTTTTTTTTGTTTTTGAGACAGAGTCTCGCTCTTTCACCCAGGCCGGACTGCAGTGGCGC
TATCTCGGCTCACTGCAAGCTCCTCCTCCCGGGTTCATGCCATTCTTCTGCCTCAACCTC
CTGAGTAGCTGGGATTACAGGCCCCCGCCACCGCGCCTGGCTATTTTTTTTTAAAAAAAA
AAAAAAACCAGNNTTNACACCTTTTTAGCCCAGAATGTCTTCGTTCTCCTGACCTCCTGA
TCTGCCCACCTTGGCCTCCCCAAGTGCTTGTATTTTTGGCATGAGCCCCCCGCCCCCGGC
CAAATAAATATTTTTCTGTAGTTGAATTGAAAATAGATTATTCTCTTTTGCTGAATTTCA
ATAATCTTATTCACTCTTTAAATTGTCCCTCCCGGAAAATTTCCAAATTTCTTTTTAACT
TGCTTTCTCAAAATTCATTTAAAATTTCCAATTTCTTTTAGCTTTTCCAATTACACTTTT
AAATTCTTAAGTCCATTATTCTTAAAAT
Restriction Sites NotI-NotI     
ACCN NM_005989
Insert Size 981 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005989.2, NP_005980.1
RefSeq Size 2692 bp
RefSeq ORF 981 bp
Locus ID 6718
UniProt ID P51857
Domains aldo_ket_red
Protein Families Druggable Genome
Protein Pathways Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways, Primary bile acid biosynthesis
Gene Summary The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (7)

The use of this cDNA Clones has been cited in the following citations:
Differential activity and expression of human 5β-reductase (AKR1D1) splice variants ,null, Journal of Molecular Endocrinology ,PubMed ID 33502336 [AKR1D1]
The A-ring reduction of 11-ketotestosterone is efficiently catalysed by AKR1D1 and SRD5A2 but not SRD5A1 ,Barnard, L;Nikolaou, N;Louw, C;Schiffer, L;Gibson, H;Gilligan, LC;Gangitano, E;Snoep, J;Arlt, W;Tomlinson, JW;Storbeck, KH;, J. Steroid Biochem. Mol. Biol. ,PubMed ID 32629108 [AKR1D1]
Differential activity and expression of human 5β-reductase (AKR1D1) splice variants ,Appanna, N;Gangitano, E;Dempster, N;Morris, K;George, S;Keevil, B;Penning, T;Gathercole, L;Tomlinson, J;Nikolaou, N;, bioRxiv [AKR1D1]
Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo ,Nikolaou, N;Arvaniti, A;Appanna, N;Sharp, A;Hughes, B;Digweed, D;Whitaker, M;Ross, RJ;Arlt, W;Penning, T;Morris, K;George, S;Keevil, B;Hodson, L;Gathercole, L;Tomlinson, JW;, J. Endocrinol. ,PubMed ID 32106090 [AKR1D1]
AKR1D1 is a novel regulator of metabolic phenotype in human hepatocytes and is dysregulated in non-alcoholic fatty liver disease ,Nikolaou, N;Gathercole, LL;Marchand, L;Althari, S;Dempster, NJ;Green, CJ;van de Bunt, M;McNeil, C;Arvaniti, A;Hughes, BA;Sgromo, B;Gillies, RS;Marschall, HU;Penning, TM;Ryan, J;Arlt, W;Hodson, L;Tomlinson, JW;, Metab. Clin. Exp. ,PubMed ID 31330134 [AKR1D1]
AKR1D1 regulates glucocorticoid availability and glucocorticoid receptor activation in human hepatoma cells ,Nikolaou, N;Gathercole, LL;Kirkwood, L;Dunford, JE;Hughes, BA;Gilligan, LC;Oppermann, U;Penning, TM;Arlt, W;Hodson, L;Tomlinson, JW;, J. Steroid Biochem. Mol. Biol. ,PubMed ID 30769091 [AKR1D1]
Genetic Variation in Aldo-Keto Reductase 1D1 (AKR1D1) Affects the Expression and Activity of Multiple Cytochrome P450s ,Amarjit S. Chaudhry, Ranjit K. Thirumaran, Kazuto Yasuda, Xia Yang, Yiping Fan, Stephen C. Strom, and Erin G. Schuetz, Drug Metab. Dispos., Aug 2013; 41: 1538 - 1547. ,PubMed ID 23704699 [AKR1D1]

Other Versions

SKU Description Size Price
RC223056 AKR1D1 (Myc-DDK-tagged)-Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1
    • 10 ug

CNY 2,400.00
CNY 3,990.00

RC223056L1 Lenti ORF clone of Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1, Myc-DDK-tagged
    • 10 ug

CNY 4,800.00
CNY 5,890.00

RC223056L2 Lenti ORF clone of Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1, mGFP tagged
    • 10 ug

CNY 5,890.00

RC223056L3 Lenti ORF clone of Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1, Myc-DDK-tagged
    • 10 ug

CNY 5,890.00

RC223056L4 Lenti ORF clone of Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1, mGFP tagged
    • 10 ug

CNY 5,890.00

RG223056 AKR1D1 (tGFP-tagged) - Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 1
    • 10 ug

CNY 4,000.00
CNY 4,370.00

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RC223056L4V

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RC223056L2V

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