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MTHFD1 (NM_005956) Human Untagged Clone
CAT＃: SC116397
MTHFD1 (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1)
CN¥ 6,856.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CIMAH; MTHFC; MTHFD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116397 sequence for NM_005956 edited (data generated by NextGen Sequencing)
ATGGCGCCAGCAGAAATCCTGAACGGGAAGGAGATCTCCGCGCAAATAAGGGCGAGACTG AAAAATCAAGTCACTCAGTTGAAGGAGCAAGTACCTGGTTTCACACCACGCCTGGCAATA TTACAGGTTGGCAACAGAGATGATTCCAATCTTTATATAAATGTGAAGCTGAAGGCTGCT GAAGAGATTGGGATCAAAGCCACTCACATTAAGTTACCAAGAACAACCACAGAATCTGAG GTGATGAAGTACATTACATCTTTGAATGAAGACTCTACTGTACATGGGTTCTTAGTGCAG CTACCTTTAGATTCAGAGAATTCCATTAACACTGAAGAAGTGATCAATGCTATTGCACCC GAGAAGGATGTGGATGGATTGACTAGCATCAATGCTGGGAGACTTGCTAGAGGTGACCTC AATGACTGTTTCATTCCTTGTACGCCTAAGGGATGCTTGGAACTCATCAAAGAGACAGGG GTGCCGATTGCCGGAAGGCATGCTGTGGTGGTTGGGCGCAGTAAAATAGTTGGGGCCCCG ATGCATGACTTGCTTCTGTGGAACAATGCCACAGTGACCACCTGCCACTCCAAGACTGCC CATCTGGATGAGGAGGTAAATAAAGGTGACATCCTGGTGGTTGCAACTGGTCAGCCTGAA ATGGTTAAAGGGGAGTGGATCAAACCTGGGGCAATAGTCATCGACTGTGGAATCAATTAT GTCCCAGATGATAAAAAACCAAATGGGAGAAAAGTTGTGGGTGATGTGGCATACGACGAG GCCAAAGAGAGGGCGAGCTTCATCACTCCTGTTCCTGGCGGCGTAGGGCCCATGACAGTT GCAATGCTCATGCAGAGCACAGTAGAGAGTGCCAAGCGTTTCCTGGAGAAATTTAAGCCA GGAAAGTGGATGATTCAGTATAACAACCTTAACCTCAAGACACCTGTTCCAAGTGACATT GATATATCACGATCTTGTAAACCGAAGCCCATTGGTAAGCTGGCTCGAGAAATTGGTCTG CTGTCTGAAGAGGTAGAATTATATGGTGAAACAAAGGCCAAAGTTCTGCTGTCAGCACTA GAACGCCTGAAGCACCGGCCTGATGGGAAATACGTGGTGGTGACTGGAATAACTCCAACA CCCCTGGGAGAAGGGAAAAGCACAACTACAATCGGGCTAGTGCAAGCCCTTGGTGCCCAT CTCTACCAGAATGTCTTTGCGTGTGTGCGACAGCCTTCTCAGGGCCCCACCTTTGGAATA AAAGGTGGCGCTGCAGGAGGCGGCTACTCCCAGGTCATTCCTATGGAAGAGTTTAATCTC CACCTCACAGGTGACATCCATGCCATCACTGCAGCTAATAACCTCGTTGCTGCGGCCATT GATGCTCGGATATTTCATGAACTGACCCAGACAGACAAGGCTCTCTTTAATCGTTTGGTG CCATCAGTAAATGGAGTGAGAAGGTTCTCTGACATCCAAATCCGAAGGTTAAAGAGACTA GGCATTGAAAAGACTGACCCTACCACACTGACAGATGAAGAGATAAACAGATTTGCAAGA TTGGACATTGATCCAGAAACCATAACTTGGCAAAGAGTGTTGGATACCAATGATAGATTC CTGAGGAAGATCACGATTGGACAGGCTCCAACGGAGAAGGGTCACACACGGACGGCCCAG TTTGATATCTCTGTGGCCAGTGAAATTATGGCTGTCCTGGCTCTCACCACTTCTCTAGAA GACATGAGAGAGAGACTGGGCAAAATGGTGGTGGCATCCAGTAAGAAAGGAGAGCCCGTC AGTGCCGAAGATCTGGGGGTGAGTGGTGCACTGACAGTGCTTATGAAGGACGCAATCAAG CCCAATCTCATGCAGACACTGGAGGGCACTCCAGTGTTTGTCCATGCTGGCCCGTTTGCC AACATCGCACATGGCAATTCCTCCATCATTGCAGACCGGATCGCACTCAAGCTTGTTGGC CCAGAAGGGTTTGTAGTGACGGAAGCAGGATTTGGAGCAGACATTGGAATGGAAAAGTTT TTTAACATCAAATGCCGGTATTCCGGCCTCTGCCCCCACGTGGTGGTGCTTGTTGCCACT GTCAGGGCTCTCAAGATGCACGGGGGCGGCCCCACGGTCACTGCTGGACTGCCTCTTCCC AAGGCTTACATACAGGAGAACCTGGAGCTGGTTGAAAAAGGCTTCAGTAACTTGAAGAAA CAAATTGAAAATGCCAGAATGTTTGGAATTCCAGTAGTAGTGGCCGTGAATGCATTCAAG ACGGATACAGAGTCTGAGCTGGACCTCATCAGCCGCCTTTCCAGAGAACATGGGGCTTTT GATGCCGTGAAGTGCACTCACTGGGCAGAAGGGGGCAAGGGTGCCTTAGCCCTGGCTCAG GCCGTCCAGAGAGCAGCACAAGCACCCAGCAGCTTCCAGCTCCTTTATGACCTCAAGCTC CCAGTTGAGGATAAAATCAGGATCATTGCACAGAAGATCTATGGAGCAGATGACATTGAA TTACTTCCCGAAGCTCAACACAAAGCTGAAGTCTACACGAAGCAGGGCTTTGGGAATCTC CCCATCTGCATGGCTAAAACACACTTGTCTTTGTCTCACAACCCAGAGCAAAAAGGTGTC CCTACAGGCTTCATTCTGCCCATTCGCGACATCCGCGCCAGCGTTGGGGCTGGTTTTCTG TACCCCTTAGTAGGAACGATGAGCACAATGCCTGGACTCCCCACCCGGCCCTGTTTTTAT GATATTGATTTGGACCCTGAAACAGAACAGGTGAATGGATTATTCTAA Clone variation with respect to NM_005956.3 401 a=>g >OriGene 5' read for NM_005956 unedited
GTCAAATTTGTATACGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGGGGAACCTCGA TATTGGTGGTGTCCATCGTGGGCAGCGGACTAATAAAGGCCATGGCGCCAGCAGAAATCC TGAACGGGAAGGAGATCTCCGCGCAAATAAGGGNCGAGAACTGAAAAATCAAGTCACTCA GTTGAAGGAGCAAGTACCTGGTTTCACACCACGCCTGGCAATATTACAGGTTGGCAACAG AGATGATTCCAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAAGAGATTGGGATCAA AGCCACTCACATTAAGTTACCAAGAACAACCACAGAATCTGAGGTGATGAAGTACATTAC ATCTTTGAATGAAGACTCTACTGTACATGGGTTCTTAGTGCAGCTACCTTTAGATTCAGA GAATTCCATTAACACTGAAGAAGTGATCAATGCTATTGCACCCGAGAAGGATGTGGATGG ATTGACTAGCATCAATGCTGGGAGACTTGCTAGAGGTGACCTCAATGACTGTTTCATTCC TTGTACGCCTAAGGGATGCTTGGAACTCATCAAAGAGACAGGGGTGCCGATTGCCGGAAG GCATGCTGTGGTGGTTGGGCGCAGTAAAATAGTTGGGGCCCCGATGCATGACTTGCTTCT GTGGAACAATGCCACAGTGACCACCTGCCACTCCAAGACTGCCCATCTGGATGAGGAGGT AAATAAAGGTGACATNCTGGTGGTTGCAACTGGTCAGCCTGAAATGTTTAAAGGGGAGTG GATCAAACCTGGNNGCATAGTCATCGACTGTGGAATCAATATGTCCCAGATGATAAAAAA CCAAATGGGAGAAAGTTGTTGGGTGATGTTGCATACGACGAGGCCACAGAGAGGGCGAGC TTCATCACTCCTGTTTCTGGCCGCGTAAGGC >OriGene 3' read for NM_005956 unedited
ACGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGATGGCAAACTTGTTTC CTTTTATTCTGTGGAACGTCACTAAAGTAAACAGACATGCATGATTTATGTTAATTTATA CATGATGAGAATTAAGGCTGAAAATGACTTCTGGGGAAACTCCTACTATTAGTTTCAGAT CTTCTGGGCAGGGGCTGACTTCTCTTGGCTTACTTATACTTCCTAACTCCCAGTGGGCCT GAATAGACACTGGCCAGACTTTCAAAGTAGCTTCTTGAAGATGGATGGTGATCTGTTTAG AATAATCCATTCACCTGTTCTGTTTCAGGGTCCAAATCAATATCATAAAAACAGGGCCGG GTGGGGAGTCCAGGCATTGTGCTCATCGTTCCTACTAAGGGGTACAGAAAACCAGCCCCA ACGCTGGCGCGGATGTCGCGAATGGGCAGAATGAAGCCTGTAGGGACACCTTTTTGCTCT GGGTTGTGAGACAAAGACAAGTGTGTTTTAGCCATGCAGATGGGGAGATTCCCAAAGCCC TGCTTCGTGTAGACTTCAGCTTTGTGTTGAGCTTCGGGAAGTAATTCAATGTCATCTGCT CCATAGATCTTCTGTGCAATGATCCTGATTTTATCCTCAACTGGGAGCTTGAGGTCATAA AGGAGCTGGAAGCTGCTGGGTGCTTGTGCTGCTCTCTGGACGGCCTGAGCCAGGGCTAAA GCACCCTTGCCCCCTTCTGCCCAGTGAGTGCACTTCACGGCATCAAAAGCCCCATGTTCT CTGGNAAGGCGGCTGATGAAGTCCAGCTCANACTCTGTATCCGTCTTTGATGCCTTCACG GCCACTACTACTGGGATTCCAACATTCTGGCATTTTCATTTGTTCTTCAAGTACTGAACC CTTTTAACCGCTCCAGTTCTCCTGTTGTTAGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005956 |
| Insert Size | 3460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005956.2, NP_005947.2 |
| RefSeq Size | 3110 bp |
| RefSeq ORF | 2808 bp |
| Locus ID | 4522 |
| UniProt ID | P11586 |
| Domains | FTHFS, THF_DHG_CYH |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate |
| Gene Summary | This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200297 | MTHFD1 (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1) |
CN¥ 6,848.00 |
|
| RC200297L3 | Lenti ORF clone of Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), Myc-DDK-tagged |
CN¥ 9,880.00 |
|
| RC200297L4 | Lenti ORF clone of Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), mGFP tagged |
CN¥ 9,248.00 |
|
| RG200297 | MTHFD1 (tGFP-tagged) - Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1) |
CN¥ 8,740.00 |
