UBE4B (NM_006048) Human Untagged Clone
CAT#: SC116377
UBE4B (untagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 2
CNY 8,176.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | E4; HDNB1; UBOX3; UFD2; UFD2A |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116377 sequence for NM_006048 edited (data generated by NextGen Sequencing)
ATGGAGGAGCTGAGCGCTGATGAGATTCGACGGAGGCGCCTTGCACGACTTGCTGGTGGA CAGACCTCTCAGCCAACCACCCCACTCACCTCTCCCCAGAGGGAGAACCCTCCGGGGCCT CCCATAGCGGCATCAGCCCCAGGACCCTCTCAGAGTCTTGGTCTCAATGTCCACAACATG ACCCCAGCTACCTCCCCAATAGGTGCATCAGGAGTAGCCCATCGAAGCCAGAGCAGTGAA GGAGTCAGTTCTCTCAGCAGCTCGCCCTCTAATAGCCTTGAAACGCAATCTCAGTCTCTC TCACGTTCCCAGAGCATGGATATCGATGGTGTCTCATGTGAGAAAAGCATGTCCCAGGTG GATGTGGATTCAGGAATTGAAAACATGGAGGTTGATGAAAATGATCGAAGAGAAAAGCGG AGCCTCAGTGATAAGGAGCCTTCCTCGGGCCCTGAAGTGTCTGAAGAGCAGGCCTTACAG CTGGTCTGTAAGATCTTCCGTGTCTCTTGGAAGGACCGGGACAGAGATGTCATCTTTCTT TCTTCTCTTTCTGCACAGTTTAAGCAGAACCCAAAAGAAGTATTCTCCGATTTTAAGGAC TTGATTGGCCAGATTTTAATGGAAGTGCTAATGATGTCCACTCAGACCAGAGATGAAAAC CCATTTGCCAGTCTGACAGCCACATCACAGCCAATTGCTGCAGCAGCACGGTCACCAGAC AGAAATCTCTTGCTAAACACTGGCTCCAATCCAGGAACAAGCCCCATGTTCTGCAGCGTG GCTTCCTTTGGTGCCAGCTCTTTGTCTAGTTTGGGAGCCTCTGGTGGAGCAAGTAATTGG GATTCCTACAGTGACCATTTCACCATTGAAACCTGCAAAGAGACAGATATGCTGAACTAC CTCATCGAGTGTTTCGACCGAGTTGGAATAGAGGAAAAAAAAGCACCAAAGATGTGCAGC CAGCCAGCAGTCAGCCAGCTTCTGAGCAACATCCGCTCACAGTGCATATCCCATACTGCT TTAGTACTACAAGGCTCCCTAACACAGCCCAGGTCCTTGCAGCAGCCGTCCTTCCTAGTG CCGTATATGCTGTGTAGGAATCTCCCATATGGCTTCATTCAGGAACTGGTGAGAACCACT CACCAGGATGAAGAAGTGTTCAAGCAGATATTTATCCCCATTTTACAAGGCCTGGCTCTT GCTGCCAAAGAGTGCTCCCTCGACAGTGACTACTTTAAATACCCCCTCATGGCACTAGGT GAGCTCTGTGAAACCAAGTTTGGGAAGACACACCCTGTGTGCAATTTGGTTGCTTCTTTG CGGTTGTGGTTGCCGAAATCCTTAAGTCCTGGCTGTGGGCGGGAGCTGCAGAGACTCTCT TACTTAGGGGCTTTCTTTAGCTTCTCAGTCTTTGCAGAAGATGATGTTAAAGTGGTTGAA AAATACTTCTCAGGGCCTGCCATTACCCTGGAAAACACTCGTGTGGTTAGCCAATCATTG CAGCATTACTTAGAGCTCGGAAGGCAAGAGCTTTTTAAGATTCTGCATAGTATTTTGTTA AATGGCGAAACCCGTGAGGCTGCTCTCAGTTACATGGCGGCTGTCGTCAATGCCAATATG AAGAAAGCACAGATGCAGACAGATGATAGATTGGTGTCTACAGATGGATTTATGCTGAAT TTCCTTTGGGTACTGCAGCAGCTAAGTACAAAAATCAAGTTAGAAACAGTTGATCCCACG TATATTTTTCACCCAAGATGTCGGATTACTCTTCCCAATGATGAGACGCGTGTGAATGCA ACGATGGAAGATGTGAATGACTGGCTGACTGAACTCTATGGCGATCAGCCTCCATTTTCT GAGCCGAAATTCCCTACGGAGTGCTTCTTTCTCACCCTGCATGCTCACCACCTCTCTATT CTGCCTAGTTGCCGTCGCTATATCCGCAGACTCCGGGCTATCCGGGAGCTCAATAGAACT GTAGAAGATTTGAAAAATAATGAAAGCCAATGGAAAGATTCCCCACTGGCAACTAGACAC CGCGAAATGCTGAAGCGCTGTAAAACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGT GCTGATGCTGGCCTACTTGACGAGAGCTTCCTGAGAAGATGTCTGAATTTTTATGGCCTT CTCATTCAGCTGCTGCTCCGCATCCTGGACCCCGCATATCCCGATATAACACTGCCTTTA AATTCAGATGTCCCCAAGGTATTTGCAGCGTTGCCTGAGTTTTATGTAGAAGATGTTGCA GAATTTTTATTTTTTATTGTACAATACTCTCCCCAGGCGCTTTATGAGCCCTGTACTCAG GATATTGTGATGTTCCTTGTTGTGATGTTGTGCAACCAGAACTACATCCGAAACCCATAT TTGGTGGCCAAACTGGTAGAAGTCATGTTTATGACCAACCCTGCTGTTCAGCCACGAACC CAGAAGTTTTTTGAAATGATTGAGAACCATCCTCTCTCCACCAAGTTGTTGGTACCTTCC CTGATGAAGTTTTATACAGATGTTGAGCATACCGGAGCCACCAGTGAGTTTTATGACAAG TTCACAATTCGCTATCATATTAGCACCATTTTTAAAAGCCTTTGGCAAAACATAGCTCAC CATGGCACCTTTATGGAGGAGTTCAACTCCGGGAAGCAGTTTGTTCGCTATATAAACATG TTGATAAACGACACGACGTTTTTGCTCGATGAAAGTCTGGAGTCTCTGAAGCGAATCCAT GAAGTGCAGGAAGAGATGAAGAACAAAGAACAGTGGGACCAGTTGCCCCGGGATCAGCAG CAGGCTCGTCAGTCTCAGCTTGCTCAGGATGAGCGTGTGTCCCGCTCTTACCTCGCCCTG GCCACCGAAACCGTGGACATGTTCCACATCCTCACGAAGCAGGTCCAGAAGCCCTTCCTC AGACCGGAGCTTGGACCCCGATTGGCTGCAATGCTGAACTTTAATCTTCAGCAACTTTGT GGCCCCAAGTGCCGTGACCTGAAAGTTGAAAACCCTGAGAAATACGGCTTTGAACCAAAG AAGCTGTTGGACCAACTGACGGATATTTACTTACAGCTGGACTGTGCTCGGTTCGCGAAA GCCATTGCTGACGACCAGAGATCCTACAGTAAGGAATTGTTTGAAGAAGTTATTTCAAAG ATGCGGAAGGCAGGGATCAAATCCACAATAGCAATAGAAAAATTTAAGCTGCTCGCCGAG AAAGTGGAGGAGATAGTGGCCAAGAACGCACGCGCAGAAATCGACTACAGCGACGCTCCT GATGAGTTCAGAGACCCTCTGATGGACACCCTCATGACAGACCCCGTGCGGCTGCCCTCT GGCACCATCATGGACCGCTCCATCATCCTGCGGCACCTGCTCAACTCCCCCACGGACCCC TTCAACCGGCAGACGCTGACAGAGAGCATGCTGGAACCAGTGCCAGAACTGAAAGAGCAG ATTCAGGCGTGGATGAGAGAGAAACAGAACAGCGATCACTAA Clone variation with respect to NM_006048.4 >OriGene 5' read for NM_006048 unedited
CGGATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACCAGGCAGGATCTG CTGTAGGAACGCAGCTACCGCGCCACTATCACGAAGAACAGCAGGCTCGGGGCACGAGAC GAACTGGAGACCGCGCTGCCTAGCTGGGTAACCTGGGAAGCAGAGGGTAATAAGTGGCGC CTTAAGACAACCCTGTAGCAGCAGCAGTGGCGGCCAAAGGAGGCTGCTCAGGGAACAAGC GGCTGTAGTAGTCTGTGGGGCGACTGGAGTGACCGAAGCCAAGGCAGTTTAGTGCCTCTC GTGTTCTTATTTTTTAACCTCTGACTATGCAATTCTGAAACCTCCCCCATTCGGGGGACC AGACAGCCTGATAGACACCTTCCACTCTCCTTCCTCCCGCCGTGGTCTCGAGAACAGAAG GATCTCTCCTTAACGCCTTTCACCATTAAGAGGAAAGCGATGGAGGAGCTGAGCGCTGAT GAGATTCGACGGAGGCGCCTTGCACGACTTGCTGGTGGACAGACCTCTCAGCCAACCACC CCACTCACCTCTCCCCAGAGGGAGAACCCTCCGGGGCCTCCCATAGCGGCATCAGCCCCA GGACCCTCTCAGAGTCTTGGTCTCAATGTCCACAACATGACCCCAGCTACCTCCCCAATA GGTGCATCAGGAGTAGCCCATCGAAGCCAGAGCAGTGAAGGAGTCAGTTCTCTCAGCAGC TCGCCCTCTAATAGCCTTTGAACGCAATCTCAGTCTCTCTCACGTTCCCAGAGCATGGAT ATCGATGGTGTCTCATGTGAGAAAAGCATGTCCCAGGTGGATGTGGATTCAGGAATTGAA ACATGGNAGGTGATGAAAATGATCGAAGAGAAAGCGGAGCCTCAGTGTAAGGAGCCTTCC TCCGGCCCTGAGTGTCTGAAGAGCAGCCCTACAGCTGTCTGGAAGACTTCCTGTCTCTA >OriGene 3' read for NM_006048 unedited
ATACTCTGGACCGGCGGCCGCATNCTAGNGTCGAGTTTTTTTTTTTTTTTTTTTTTCTGA AAAATCTTGCCTTTTTTATTAATCATGTCATCTAACACCAATTAAAAAAACAATATGTGA ATATGATATAAAAATACGATCCCAGGATTAACACTTGGTAGCAGGCACAGTTATCTCACA GAATATATGTTTGGAGGGCCAGGGAAAGGAGTTTACTTTTGTTTTTGCTTCTCTTAAAAA AATCCACAGCAGTGCAAGTAGGGTCAGGAAGGGGGAGAGGGGAAAAAAGATTCTTTAGCA GCAAAATGTCCAAGGTTCTCTAATCACAATCAAATCAACGGATATTGGATTAAAAAAAAA TTCATGAAGGGATGCTGCCCAGACCCATTAGCCATCACTAAAAGACTTCCGTTGCCCCAA ACTGTGACATACGAAGGACATGCTTTATTACACAAGCAACCATAAAACCTGTCCCTTAAG CTTTTGACCGTGTTTGTCACTTAAATATATGTACAGGAAGCGGGCTCCTCTTCTCATCCA TGTCCTTTCAGGTCTCAGCGTTTGCTCGCTCTGGGTGGGCCTGGGGTTGGTTTGCCACAT TTGGCCTCCACACATGAACGGCAGCTTCGCTGCGGCCGCTGCTTCTGCTTGCCTCGTTGG CCTTGGCTGTGTCTAGCAGAGGGTGGGCGGCGGAACGGTTTANNGATCGCTGNTCTGTTT CTCTCTCATCCACGCCTGAATCTGCTCTTTCAGTTCTGGCACTGGTTCCAGCATGCTCTC TGTCANCGTCTGCCGTTGAAGGGGTCCGTGGGGGATTGANCCAGTGCCGCAAGATGATGA ACGTCCATGATGGTGCAAAGGGCANCCGAACGGGTCTGCATGAAGGTGTCATCAAAGGTC CTTGACTCATCAGAGCGCCCTGTATCCATTCTGGCGCGGCGTTTTGCACCTATTTCCC |
Restriction Sites | NotI-NotI |
ACCN | NM_006048 |
Insert Size | 4600 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006048.2, NP_006039.2 |
RefSeq Size | 5512 bp |
RefSeq ORF | 3522 bp |
Locus ID | 10277 |
UniProt ID | O95155 |
Domains | U-box |
Protein Pathways | Ubiquitin mediated proteolysis |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region of the CDS, and thus encodes a shorter isoform (2), as compared to variant 1. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210800 | UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 2 |
CNY 8,168.00 |
|
RC210800L3 | Lenti-ORF clone of UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 2 |
CNY 13,590.00 |
|
RC210800L4 | Lenti-ORF clone of UBE4B (mGFP-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 2 |
CNY 13,590.00 |
|
RG210800 | UBE4B (tGFP-tagged) - Human ubiquitination factor E4B (UBE4B), transcript variant 2 |
CNY 12,830.00 |