UBE4B (NM_006048) Human Untagged Clone

CAT#: SC116377

UBE4B (untagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 2



  "NM_006048" in other vectors (4)

CNY 8,176.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


UBE4B rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms E4; HDNB1; UBOX3; UFD2; UFD2A
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116377 sequence for NM_006048 edited (data generated by NextGen Sequencing)
ATGGAGGAGCTGAGCGCTGATGAGATTCGACGGAGGCGCCTTGCACGACTTGCTGGTGGA
CAGACCTCTCAGCCAACCACCCCACTCACCTCTCCCCAGAGGGAGAACCCTCCGGGGCCT
CCCATAGCGGCATCAGCCCCAGGACCCTCTCAGAGTCTTGGTCTCAATGTCCACAACATG
ACCCCAGCTACCTCCCCAATAGGTGCATCAGGAGTAGCCCATCGAAGCCAGAGCAGTGAA
GGAGTCAGTTCTCTCAGCAGCTCGCCCTCTAATAGCCTTGAAACGCAATCTCAGTCTCTC
TCACGTTCCCAGAGCATGGATATCGATGGTGTCTCATGTGAGAAAAGCATGTCCCAGGTG
GATGTGGATTCAGGAATTGAAAACATGGAGGTTGATGAAAATGATCGAAGAGAAAAGCGG
AGCCTCAGTGATAAGGAGCCTTCCTCGGGCCCTGAAGTGTCTGAAGAGCAGGCCTTACAG
CTGGTCTGTAAGATCTTCCGTGTCTCTTGGAAGGACCGGGACAGAGATGTCATCTTTCTT
TCTTCTCTTTCTGCACAGTTTAAGCAGAACCCAAAAGAAGTATTCTCCGATTTTAAGGAC
TTGATTGGCCAGATTTTAATGGAAGTGCTAATGATGTCCACTCAGACCAGAGATGAAAAC
CCATTTGCCAGTCTGACAGCCACATCACAGCCAATTGCTGCAGCAGCACGGTCACCAGAC
AGAAATCTCTTGCTAAACACTGGCTCCAATCCAGGAACAAGCCCCATGTTCTGCAGCGTG
GCTTCCTTTGGTGCCAGCTCTTTGTCTAGTTTGGGAGCCTCTGGTGGAGCAAGTAATTGG
GATTCCTACAGTGACCATTTCACCATTGAAACCTGCAAAGAGACAGATATGCTGAACTAC
CTCATCGAGTGTTTCGACCGAGTTGGAATAGAGGAAAAAAAAGCACCAAAGATGTGCAGC
CAGCCAGCAGTCAGCCAGCTTCTGAGCAACATCCGCTCACAGTGCATATCCCATACTGCT
TTAGTACTACAAGGCTCCCTAACACAGCCCAGGTCCTTGCAGCAGCCGTCCTTCCTAGTG
CCGTATATGCTGTGTAGGAATCTCCCATATGGCTTCATTCAGGAACTGGTGAGAACCACT
CACCAGGATGAAGAAGTGTTCAAGCAGATATTTATCCCCATTTTACAAGGCCTGGCTCTT
GCTGCCAAAGAGTGCTCCCTCGACAGTGACTACTTTAAATACCCCCTCATGGCACTAGGT
GAGCTCTGTGAAACCAAGTTTGGGAAGACACACCCTGTGTGCAATTTGGTTGCTTCTTTG
CGGTTGTGGTTGCCGAAATCCTTAAGTCCTGGCTGTGGGCGGGAGCTGCAGAGACTCTCT
TACTTAGGGGCTTTCTTTAGCTTCTCAGTCTTTGCAGAAGATGATGTTAAAGTGGTTGAA
AAATACTTCTCAGGGCCTGCCATTACCCTGGAAAACACTCGTGTGGTTAGCCAATCATTG
CAGCATTACTTAGAGCTCGGAAGGCAAGAGCTTTTTAAGATTCTGCATAGTATTTTGTTA
AATGGCGAAACCCGTGAGGCTGCTCTCAGTTACATGGCGGCTGTCGTCAATGCCAATATG
AAGAAAGCACAGATGCAGACAGATGATAGATTGGTGTCTACAGATGGATTTATGCTGAAT
TTCCTTTGGGTACTGCAGCAGCTAAGTACAAAAATCAAGTTAGAAACAGTTGATCCCACG
TATATTTTTCACCCAAGATGTCGGATTACTCTTCCCAATGATGAGACGCGTGTGAATGCA
ACGATGGAAGATGTGAATGACTGGCTGACTGAACTCTATGGCGATCAGCCTCCATTTTCT
GAGCCGAAATTCCCTACGGAGTGCTTCTTTCTCACCCTGCATGCTCACCACCTCTCTATT
CTGCCTAGTTGCCGTCGCTATATCCGCAGACTCCGGGCTATCCGGGAGCTCAATAGAACT
GTAGAAGATTTGAAAAATAATGAAAGCCAATGGAAAGATTCCCCACTGGCAACTAGACAC
CGCGAAATGCTGAAGCGCTGTAAAACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGT
GCTGATGCTGGCCTACTTGACGAGAGCTTCCTGAGAAGATGTCTGAATTTTTATGGCCTT
CTCATTCAGCTGCTGCTCCGCATCCTGGACCCCGCATATCCCGATATAACACTGCCTTTA
AATTCAGATGTCCCCAAGGTATTTGCAGCGTTGCCTGAGTTTTATGTAGAAGATGTTGCA
GAATTTTTATTTTTTATTGTACAATACTCTCCCCAGGCGCTTTATGAGCCCTGTACTCAG
GATATTGTGATGTTCCTTGTTGTGATGTTGTGCAACCAGAACTACATCCGAAACCCATAT
TTGGTGGCCAAACTGGTAGAAGTCATGTTTATGACCAACCCTGCTGTTCAGCCACGAACC
CAGAAGTTTTTTGAAATGATTGAGAACCATCCTCTCTCCACCAAGTTGTTGGTACCTTCC
CTGATGAAGTTTTATACAGATGTTGAGCATACCGGAGCCACCAGTGAGTTTTATGACAAG
TTCACAATTCGCTATCATATTAGCACCATTTTTAAAAGCCTTTGGCAAAACATAGCTCAC
CATGGCACCTTTATGGAGGAGTTCAACTCCGGGAAGCAGTTTGTTCGCTATATAAACATG
TTGATAAACGACACGACGTTTTTGCTCGATGAAAGTCTGGAGTCTCTGAAGCGAATCCAT
GAAGTGCAGGAAGAGATGAAGAACAAAGAACAGTGGGACCAGTTGCCCCGGGATCAGCAG
CAGGCTCGTCAGTCTCAGCTTGCTCAGGATGAGCGTGTGTCCCGCTCTTACCTCGCCCTG
GCCACCGAAACCGTGGACATGTTCCACATCCTCACGAAGCAGGTCCAGAAGCCCTTCCTC
AGACCGGAGCTTGGACCCCGATTGGCTGCAATGCTGAACTTTAATCTTCAGCAACTTTGT
GGCCCCAAGTGCCGTGACCTGAAAGTTGAAAACCCTGAGAAATACGGCTTTGAACCAAAG
AAGCTGTTGGACCAACTGACGGATATTTACTTACAGCTGGACTGTGCTCGGTTCGCGAAA
GCCATTGCTGACGACCAGAGATCCTACAGTAAGGAATTGTTTGAAGAAGTTATTTCAAAG
ATGCGGAAGGCAGGGATCAAATCCACAATAGCAATAGAAAAATTTAAGCTGCTCGCCGAG
AAAGTGGAGGAGATAGTGGCCAAGAACGCACGCGCAGAAATCGACTACAGCGACGCTCCT
GATGAGTTCAGAGACCCTCTGATGGACACCCTCATGACAGACCCCGTGCGGCTGCCCTCT
GGCACCATCATGGACCGCTCCATCATCCTGCGGCACCTGCTCAACTCCCCCACGGACCCC
TTCAACCGGCAGACGCTGACAGAGAGCATGCTGGAACCAGTGCCAGAACTGAAAGAGCAG
ATTCAGGCGTGGATGAGAGAGAAACAGAACAGCGATCACTAA

Clone variation with respect to NM_006048.4
>OriGene 5' read for NM_006048 unedited
CGGATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACCAGGCAGGATCTG
CTGTAGGAACGCAGCTACCGCGCCACTATCACGAAGAACAGCAGGCTCGGGGCACGAGAC
GAACTGGAGACCGCGCTGCCTAGCTGGGTAACCTGGGAAGCAGAGGGTAATAAGTGGCGC
CTTAAGACAACCCTGTAGCAGCAGCAGTGGCGGCCAAAGGAGGCTGCTCAGGGAACAAGC
GGCTGTAGTAGTCTGTGGGGCGACTGGAGTGACCGAAGCCAAGGCAGTTTAGTGCCTCTC
GTGTTCTTATTTTTTAACCTCTGACTATGCAATTCTGAAACCTCCCCCATTCGGGGGACC
AGACAGCCTGATAGACACCTTCCACTCTCCTTCCTCCCGCCGTGGTCTCGAGAACAGAAG
GATCTCTCCTTAACGCCTTTCACCATTAAGAGGAAAGCGATGGAGGAGCTGAGCGCTGAT
GAGATTCGACGGAGGCGCCTTGCACGACTTGCTGGTGGACAGACCTCTCAGCCAACCACC
CCACTCACCTCTCCCCAGAGGGAGAACCCTCCGGGGCCTCCCATAGCGGCATCAGCCCCA
GGACCCTCTCAGAGTCTTGGTCTCAATGTCCACAACATGACCCCAGCTACCTCCCCAATA
GGTGCATCAGGAGTAGCCCATCGAAGCCAGAGCAGTGAAGGAGTCAGTTCTCTCAGCAGC
TCGCCCTCTAATAGCCTTTGAACGCAATCTCAGTCTCTCTCACGTTCCCAGAGCATGGAT
ATCGATGGTGTCTCATGTGAGAAAAGCATGTCCCAGGTGGATGTGGATTCAGGAATTGAA
ACATGGNAGGTGATGAAAATGATCGAAGAGAAAGCGGAGCCTCAGTGTAAGGAGCCTTCC
TCCGGCCCTGAGTGTCTGAAGAGCAGCCCTACAGCTGTCTGGAAGACTTCCTGTCTCTA
>OriGene 3' read for NM_006048 unedited
ATACTCTGGACCGGCGGCCGCATNCTAGNGTCGAGTTTTTTTTTTTTTTTTTTTTTCTGA
AAAATCTTGCCTTTTTTATTAATCATGTCATCTAACACCAATTAAAAAAACAATATGTGA
ATATGATATAAAAATACGATCCCAGGATTAACACTTGGTAGCAGGCACAGTTATCTCACA
GAATATATGTTTGGAGGGCCAGGGAAAGGAGTTTACTTTTGTTTTTGCTTCTCTTAAAAA
AATCCACAGCAGTGCAAGTAGGGTCAGGAAGGGGGAGAGGGGAAAAAAGATTCTTTAGCA
GCAAAATGTCCAAGGTTCTCTAATCACAATCAAATCAACGGATATTGGATTAAAAAAAAA
TTCATGAAGGGATGCTGCCCAGACCCATTAGCCATCACTAAAAGACTTCCGTTGCCCCAA
ACTGTGACATACGAAGGACATGCTTTATTACACAAGCAACCATAAAACCTGTCCCTTAAG
CTTTTGACCGTGTTTGTCACTTAAATATATGTACAGGAAGCGGGCTCCTCTTCTCATCCA
TGTCCTTTCAGGTCTCAGCGTTTGCTCGCTCTGGGTGGGCCTGGGGTTGGTTTGCCACAT
TTGGCCTCCACACATGAACGGCAGCTTCGCTGCGGCCGCTGCTTCTGCTTGCCTCGTTGG
CCTTGGCTGTGTCTAGCAGAGGGTGGGCGGCGGAACGGTTTANNGATCGCTGNTCTGTTT
CTCTCTCATCCACGCCTGAATCTGCTCTTTCAGTTCTGGCACTGGTTCCAGCATGCTCTC
TGTCANCGTCTGCCGTTGAAGGGGTCCGTGGGGGATTGANCCAGTGCCGCAAGATGATGA
ACGTCCATGATGGTGCAAAGGGCANCCGAACGGGTCTGCATGAAGGTGTCATCAAAGGTC
CTTGACTCATCAGAGCGCCCTGTATCCATTCTGGCGCGGCGTTTTGCACCTATTTCCC
Restriction Sites NotI-NotI     
ACCN NM_006048
Insert Size 4600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006048.2, NP_006039.2
RefSeq Size 5512 bp
RefSeq ORF 3522 bp
Locus ID 10277
UniProt ID O95155
Domains U-box
Protein Pathways Ubiquitin mediated proteolysis
Gene Summary The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region of the CDS, and thus encodes a shorter isoform (2), as compared to variant 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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