HS3ST3B1 (NM_006041) Human Untagged Clone
CAT#: SC116374
HS3ST3B1 (untagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1)
CNY 3,656.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | 3-OST-3B; 3OST3B1; h3-OST-3B |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116374 sequence for NM_006041 edited (data generated by NextGen Sequencing)
ATGGGGCAGCGCCTGAGTGGCGGCAGATCTTGCCTCGATGTCCCCGGCCGGCTCCTACCG CAGCCGCCGCCGCCCCCGCCGCCGGTGAGGAGGAAGCTCGCGCTGCTCTTCGCCATGCTC TGCGTCTGGCTCTATATGTTCCTGTACTCGTGCGCCGGCTCCTGCGCCGCCGCGCCGGGG CTGCTGCTCCTGGGCTCTGGGTCCCGCGCCGCACACGACCCGCCAGCCCTGGCCACAGCT CCGGACGGGACGCCCCCCAGGCTGCCGTTCCGGGCGCCGCCAGCCACCCCACTGGCTTCA GGCAAGGAGATGGCCGAGGGCGCTGCGAGCCCGGAGGAGCAGAGTCCCGAGGTGCCGGAC TCCCCAAGCCCCATCTCCAGCTTTTTCAGTGGGTCTGGGAGCAAGCAGCTGCCGCAGGCC ATCATCATCGGCGTGAAGAAGGGCGGCACGCGGGCGCTGCTGGAGTTTCTGCGCGTGCAC CCCGACGTGCGCGCCGTGGGCGCCGAGCCCCATTTCTTCGATCGCAGCTACGACAAGGGC CTCGCTTGGTACCGGGACCTGATGCCCAGAACCCTGGACGGGCAGATCACCATGGAGAAG ACGCCCAGTTACTTCGTCACGCGGGAGGCCCCCGCGCGCATCTCGGCCATGTCCAAGGAC ACCAAGCTCATCGTGGTGGTGCGGGACCCGGTGACCAGGGCCATCTCGGACTACACGCAG ACGCTGTCCAAGCGGCCCGACATCCCCACCTTCGAGAGCTTGACGTTCAAAAACAGGACA GCGGGCCTCATCGACACGTCGTGGAGCGCCATCCAGATCGGCATCTACGCCAAGCACCTG GAGCACTGGCTGCGCCACTTCCCCATCCGCCAGATGCTCTTCGTGAGCGGCGAGCGGCTC ATCAGCGACCCGGCCGGGGAGCTGGGCCGCGTGCAAGACTTCCTGGGCCTCAAGAGGATC ATCACGGACAAGCACTTCTACTTCAACAAGACCAAGGGCTTCCCCTGCCTGAAGAAGGCG GAGGGCAGCAGCCGGCCCCATTGCCTGGGCAAGACCAAGGGCAGGACCCATCCTGAGATC GACCGCGAGGTGGTGCGCAGGCTGCGCGAGTTCTACCGGCCTTTCAACCTCAAGTTCTAC CAGATGACCGGGCACGACTTTGGCTGGGATTGA Clone variation with respect to NM_006041.1 >OriGene 5' read for NM_006041 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGGGGAAGTGCCGGG GCTGCTCGAGGCTCATTTCTTATGACTGCAAGGAGGCAGCCCCGGCGTGCGGCGGTGCGC ACAGTCTATAGTGGCCAGGGCGCGAGAGTGCAACGTCCTCCTGGCCCCGAGCGCGTCGTC GCGCCCCGGGAGCAGACCCTCGCCCAGCAGTTACCGCCGTCCCGACTTTCCGTTCCAGTT GCAGCTCCTGCCGGGCAACATGTCAAGATCCGCCGCCGCTACAGCTGCCGCCGCCACCTG GGGAAGATCAGCAGCAGCAGCGGCGGCCGCGGGCACACGGGGGCAATAAACCGATCCACC CGGGCGTCCAGCGTGCCGGGGAACCCTCTCTGCGCTCACTGCCCGGCGGGACCCACGCCA TGTGCTGAGCCATGTCCCTGGCCGCGCCCGCGGGCAGCGCATGGGGCAGCGCCTGAGTGG CGGCAGATCTTGCCTCGATGTCCCCGGCCGGCTCCTACCGCAGCCGCCGCCGCCCCCGCC GCCGGTGAGGATGAAGCTCGCGCTGCTCTTCGCCATGCTCTGCGTCTGGCTCTATATGTT CCTGTACTCGTGCGCCGGCTCCTGCGCCGCCGCGCCGGGGCTGCTGCTCCTGAGCTCTGG GTCCCGCGCCGNACACGACCCGNCAGCCCTGGCCACAGCTNCGGACGGGACGCCCNCCAT GCTGNCGTTCCAGTCGCCGNCAGCCACCCCACTGGCTTCATGCATGGATATGGCCGATGG CGCTGCGAGCCCCGATGATCATAGTCCCGATGTGCCGGACTCCCCATGCCCATCTNCAGC TTTTTCATGGGGTCTGGGAGTCAGCAGCTGGCGCATGCCATCTCATTCGCGTGATTAATG GCGNACGCGGNCGCTGCTGNAGTTTTTGCGCGGCACCCCGACTGCGCCCGTGGTCGCGAG CCCATTTTTGGTTGCACTTCGATAGGCCTCCTTGGTCCGGNACTGATGCAAACCCGNACG GCGATACCATGGAATACCC |
Restriction Sites | NotI-NotI |
ACCN | NM_006041 |
Insert Size | 3000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006041.1, NP_006032.1 |
RefSeq Size | 2032 bp |
RefSeq ORF | 1173 bp |
Locus ID | 9953 |
UniProt ID | Q9Y662 |
Domains | Sulfotransfer |
Protein Families | Transmembrane |
Protein Pathways | Glycosaminoglycan degradation, Heparan sulfate biosynthesis |
Gene Summary | The protein encoded by this gene is a type II integral membrane protein that belongs to the 3-O-sulfotransferases family. These proteins catalyze the addition of sulfate groups at the 3-OH position of glucosamine in heparan sulfate. The substrate specificity of individual members of the family is based on prior modification of the heparan sulfate chain, thus allowing different members of the family to generate binding sites for different proteins on the same heparan sulfate chain. Following treatment with a histone deacetylase inhibitor, expression of this gene is activated in a pancreatic cell line. The increased expression results in promotion of the epithelial-mesenchymal transition. In addition, the modification catalyzed by this protein allows herpes simplex virus membrane fusion and penetration. A very closely related homolog with an almost identical sulfotransferase domain maps less than 1 Mb away. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) represents the longer transcript. It encodes the protein. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209249 | HS3ST3B1 (Myc-DDK-tagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1) |
CNY 3,656.00 |
|
RC209249L1 | Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC209249L2 | Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1), mGFP tagged |
CNY 5,890.00 |
|
RC209249L3 | Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC209249L4 | Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1), mGFP tagged |
CNY 5,890.00 |
|
RG209249 | HS3ST3B1 (tGFP-tagged) - Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1) |
CNY 4,370.00 |
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