PIAS3 (NM_006099) Human Untagged Clone

CAT#: SC116323

PIAS3 (untagged)-Human protein inhibitor of activated STAT, 3 (PIAS3)



  "NM_006099" in other vectors (7)

CNY 4,616.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PIAS3 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ZMIZ5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006099, the custom clone sequence may differ by one or more nucleotides


ATGGCGGAGCTGGGCGAATTAAAGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTG
GCTTTGCTGGCCGGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCCTGAAGTC
CAGCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCTTTCCCCGGAAGACCCTG
GGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCTGGTCCTCTAG
CTCCCATTCCCCCAACGCTGTTGGCCCCTGGCACCCTGCTGGGCCCCAAGCGTGAGGTGGACATGCACCC
CCCTCTGCCCCAGCCTGTGCACCCTGATGTCACCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAG
CTCATCCGGCCCACCACCCTTGCATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCC
TCACACCCCAGCAAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTGATTATAC
CATACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGGAAGATTATTTTCCCCCCAAC
CTCTTTGTCAAGGTCAATGGGAAACTGTGCCCCCTGCCGGGTTACCTTCCCCCAACCAAGAATGGGGCCG
AGCCCAAGAGGCCCAGCCGCCCCATCAACATCACACCCCTGGCTCGACTCTCAGCCACTGTTCCCAACAC
CATTGTGGTCAATTGGTCATCTGAGTTCGGACGGAATTACTCCTTGTCTGTGTACCTGGTGAGGCAGTTG
ACTGCAGGAACCCTTCTACAAAAACTCAGAGCAAAGGGTATCCGGAACCCAGACCACTCGCGGGCACTGA
TCAAGGAGAAATTGACTGCTGACCCTGACAGTGAGGTGGCCACTACAAGTCTCCGGGTGTCACTCATGTG
CCCGCTAGGGAAGATGCGCCTGACTGTCCCTTGTCGTGCCCTCACCTGCGCCCACCTGCAGAGCTTCGAT
GCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCTACATGGACATGTCCTGTGTGTGACAAGAAGGCTC
CCTATGAATCTCTTATCATTGATGGTTTATTTATGGAGATTCTTAGTTCCTGTTCAGATTGTGATGAGAT
CCAATTCATGGAAGATGGATCCTGGTGCCCAATGAAACCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCG
CCAGGGTATGGGCTGGATGGCCTCCAGTACAGCCCAGTCCAGGGGGGAGATCCATCAGAGAATAAGAAGA
AGGTCGAAGTTATTGACTTGACAATAGAAAGCTCATCAGATGAGGAGGATCTGCCCCCTACCAAGAAGCA
CTGTTCTGTCACCTCAGCTGCCATCCCGGCCCTACCTGGAAGCAAAGGAGTCCTGACATCTGGCCACCAG
CCATCCTCGGTGCTAAGGAGCCCTGCTATGGGCACGTTGGGTGGGGATTTCCTGTCCAGTCTCCCACTAC
ATGAGTACCCACCTGCCTTCCCACTGGGAGCCGACATCCAAGGTTTAGATTTATTTTCATTTCTTCAGAC
AGAGAGTCAGCACTATGGCCCCTCTGTCATCACCTCACTAGATGAACAGGATGCCCTTGGCCACTTCTTC
CAGTACCGAGGGACCCCTTCTCACTTTCTGGGCCCACTGGCCCCCACGCTGGGGAGCTCCCACTGCAGCG
CCACTCCGGCGCCCCCTCCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGGGGGGCCTTGAGGGAGGGGCA
TGGAGGACCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACATCATTTCCCTGGACTGA


>OriGene 5' read for NM_006099 unedited
TATATTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCCCCATTCCCC
CACCTCTCCAGCTCGCCCTCTGAGCCTCCCGAGCCCTCTCTCCATTTCCCACAATTGTGC
TGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTGGCTTTGCTGGCC
GGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCCTGAAGTCCA
GCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCTTTCCCCGGA
AGACCCTGGGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCTCTCCTGTAG
GCTCCCCTGGTCCTCTAGCTCCCATTCCCCCAACGCTGTTGGCCCCTGGCACCCTGCTGG
GCCCCAAGCGTGAGGTGGACATGCACCCCCCTCTGCCCCAGCCTGTGCACCCTGATGTCA
CCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGGCCCACCACCCTTG
CATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCCTCACACCCCAGC
AAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTGATTATACCA
TACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGNAAGATTATTTTC
CCCCCAACCTCTNTGTCAAGGTCAATGGGAAACTGTGCCCNNCTGCCGGTTACCTTCCCC
CACCAAGAATGGNGCCGAGCCCCAGAAGCCCAGCCGNCCCCATCACATCACACCCCTGGC
TCGACTCTCAGNCCTGGTCCCACACCATTTGTGTCATGGNTCATCTGAGTTCGNACGGAA
ATACTCTTGTCTGTGTACCTGGNGAGGCAGNTGACTGCAGGAACCCTTCTACAA
>OriGene 3' read for NM_006099 unedited
GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACATTCA
CAACCTTTATTATGGGTGAGAGCTTCACTACAACTTTAGAAATAAAAAGTAAAATTACAA
CATAGGTCCTAAGCCTTGGCGAGCCTGAAAAAGAAGATTGGGAAGGAGGGCACAGGGTCC
TTCCACCTCCCTGGAAAGGTGCAGAATGAGCCAGGCCTAACTACAGGGCCATGAGCCTCT
AAAAGCTTAGGGGGGAATAAGAAACACTGGGAACTTAGATATATAAATAGAGAGAGACCC
AAGCAATAGGCCGGGCCTGACTCCCAGACCCCTGCAGATCATGGCTAAGGCCTTACATCC
TCCTCTGTCATACTGGAGTAGGAGGCAGGGGAAGTCATCTTTGGAGTATTTTGGTTTTTC
TTATTTATGTACAAAAAAGTCCACATCCACAATCCAAAATAAAGTTCTCTGTCCATCTCT
GAAACTGCCTGTCTCAGTTTTTCCACCTGTCTGTTTGGCCGAAGGGAATGGAATATACAT
GAAGAGACAATGGATGTGAAGAAGAAATAGAGCCATGGGTTCGGGTAATAAGAAGAGAGA
GCTTTTGGGGTTCAAGAGAGGTGCCCCTTCCCCAAGAGGCTCTAGACATAATCAAGGCTG
AAGGTTCAACCCCTTTTCTAGCTCAATACCATCCCATTTGCCTCCCACTGCCTAGGTAAA
CTACCCAAGGAATGTGCCCCCCTCTAAAGACATTTCCAGAACAGACCCAAGGGCTTAAAA
GAGGCTAAATTTAACCCTTTGGTGCTGGCCTTTCCGGCAAAATAGCCCAAAGTAGGTTTC
TGGAAGGCTTCTGGCCCTGGGCACACCCCCAAGGAACATAGAACTGGGTTGGACCCCCAA
TACTTCCTCAAGGTGGGCAACAAAATTTCCTACCCGGGACCCTTCGGGC
Restriction Sites NotI-NotI     
ACCN NM_006099
Insert Size 2870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006099.2, NP_006090.1
RefSeq Size 2902 bp
RefSeq ORF 1860 bp
Locus ID 10401
UniProt ID Q9Y6X2
Domains SAP, zf-MIZ
Protein Families Transcription Factors
Protein Pathways Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
Gene Summary This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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