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cGKI (PRKG1) (NM_006258) Human Untagged Clone
CAT＃: SC116252
PRKG1 (untagged)-Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2
CNY 5,032.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_006258 edited
CCGGAGGAGCATGGGCACCTTGCGGGATTTACAGTACGCGCTCCAGGAGAAGATCGAGGA GCTGAGGCAGCGGGATGCTCTCATCGACGAGCTGGAGCTGGAGTTGGATCAGAAGGACGA ACTGATCCAGAAGCTGCAGAACGAGCTGGACAAGTACCGCTCGGTGATCCGACCAGCCAC CCAGCAGGCGCAGAAGCAGAGCGCGAGCACCTTGCAGGGCGAGCCGCGCACCAAGCGGCA GGCGATCTCCGCCGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCATGTGACCCTGCC CTTCTACCCCAAGAGCCCACAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGA CTTTATGAAGAACTTGGAGCTGTCGCAGATCCAGGAGATTGTGGATTGTATGTACCCGGT GGAGTATGGCAAGGACAGTTGCATCATCAAAGAAGGAGACGTGGGGTCACTGGTGTATGC CATGGAAGATGGTAAGGTTGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCC AGGAAAAGTGTTTGGGGAATTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAA GACTCTTGTAAATGTAAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATAATGAT GAGGACAGGACTCATCAAGCATACCGAGTATATGGAATTTTTAAAAAGCGTTCCAACATT CCAGAGCCTTCCTGAAGAGATCCTCAGCAAGCTTGCTGATGTCCTTGAAGAGACCCACTA TGAAAATGGAGAATATATTATCAGGCAAGGTGCAAGAGGGGACACCTTCTTTATCATCAG CAAAGGAACGGTAAATGTCACTCGTGAAGACTCACCGAGTGAAGACCCAGTCTTTCTTAG AACTTTAGGAAAAGGAGACTGGTTTGGAGAGAAAGCCTTGCAGGGGGAAGATGTGAGAAC AGCAAACGTAATTGCTGCAGAAGCTGTAACCTGCCTTGTGATTGACAGAGACTCTTTTAA ACATTTGATTGGAGGGCTGGATGATGTTTCTAATAAAGCATATGAAGATGCAGAAGCTAA AGCAAAATATGAAGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCTGTCTGATTTCAACAT CATTGATACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAACTGGTCCAGTTGAAAAGTGA AGAATCCAAAACGTTTGCAATGAAGATTCTCAAGAAACGTCACATTGTGGACACAAGACA GCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTCATAGT GAGACTGTACAGAACATTTAAGGACAGCAAATATTTGTATATGTTGATGGAAGCTTGTCT AGGTGGAGAGCTCTGGACCATTCTCAGGGATAGAGGTTCGTTTGAAGATTCTACAACCAG ATTTTACACAGCATGTGTGGTAGAAGCTTTTGCCTATCTGCATTCCAAAGGAATCATTTA CAGGGACCTCAAGCCAGAAAATCTCATCCTAGATCACCGAGGTTATGCCAAACTGGTTGA TTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCC AGAGTATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCCGACTACTG GTCACTGGGAATCCTAATGTATGAACTCCTGACTGGCAGCCCACCTTTCTCAGGCCCAGA TCCTATGAAAACCTATAACATCATATTGAGGGGGATTGACATGATAGAATTTCCAAAGAA GATTGCCAAAAATGCTGCTAATTTAATTAAAAAACTATGCAGGGACAATCCATCAGAAAG ATTAGGGAATTTGAAAAATGGAGTAAAAGACATTCAAAAGCACAAATGGTTTGAGGGCTT TAACTGGGAAGGCTTAAGAAAAGGTACCTTGACACCTCCTATAATACCAAGTGTTGCATC ACCCACAGACACAAGTAATTTTGACAGTTTCCCTGAGGACAACGATGAACCACCACCTGA TGACAACTCAGGATGGGATATAGACTTCTAATGTATTTCTCTTACCTGCTTCTGCCTTGC TGAAGACAGCTTTTTCTGAGACACAGCTGCCAGCAAACCTGAGGGAAAGAGAGAAGATTA GTGCTCGGGGTCACCATGATGCCTTTGATCGATGCTGCTCCAGTAACTACAGTGGCATTA GGACTTACCGCTTAGATGACAATAGTGCTCTTTACATGTTTTCTGTTTGAACCTAAAATA GCAGTTGACATGGTGGTCCTGAAGCAAAGCCTTTCACCAGTAAAAGATGTTTTCTATTGT TGCAATGACCTTGCTTTGCTCTGATTATAATTTGAAAGACTGTAGGAAACACTTCAATGT AGTATAAGAGTCTGTACCTTGCTGGAATATTCAAGAAGATGAAAGAATAATATATTGGGT ACAATAGATTACTATGGTACAGAAACTGGGCTATTCCCTTTCTTCAAGTGAAGGCTGTGG GATCTATTACTGCAGGCCGGTGTATATACCATACAAAAGAGGACCACACATCTGTTGGTC ACAGAGTTCATGTCACACCAGTGCTAGAAGTTTCATGA >OriGene 5' read for NM_006258 unedited
AGTTTCTTTTGTATACACCCTTAGGCGGCCGGATTCGCACGAGACGGGTGGGGAGGAAGC CTCAATACGCGGAGCAGCGGCAGGAAGGAGCCCCCGGCAGCCCGGAGGAGCATGGGCACC TTGCGGGATTTACAGTACGCGCTCCATGAGAAGATCGAGGAGCTGAGGCAGCGGGATGCT CTCATCGACGAGCTGGAGCTGGAGTTGGATCATAAGGACGAACTGATCCAGAAGCTGCAG AACGAGCTGGACAAGTACCGCTCGGTGATCCGACCAGCCACCCATCAGGCGCATAATCAG ATCGCGAGCACCTTGCATGGCGAGCCGCGCACCAAGCGGCAGGCGATCTCCGCCGAGCCC ACCGCCTTCGACATCCAGGATCTCAGCCATGTGACCCTGCCCTTCTACCCCAAGAGCCCA CAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGACTTTATGAAGAACTTGGAG CTGTCGCATATCCAGGAGATTGTGGATTGTATGTACCCGGTGGAGTATGGCAAGGACAGG TGCATCATCAAAGAATGAGACGTGGGGTCACTGGTGTATGCCATGGAAGATGGTAAGGTC TGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCCAGGATAAGTGTTTGGGGG AATTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTGAATGGAA AACTCTGGGCCATTGATCGACAATGGTTTCAAACNATAATGATGAGGACAGGACTCATCA AGCATACCGAGTATATGGAATTTTTAAAAAGCGTGCCACATTCCAGAGCCTTCCTGNAGA GATCCTCANCAAGCGTGCTGATGTCCTTGAAAAGACCACTATGGAAATGGGGATATATTA TCAGGCCAGGGGGCAGAAGGGACA |
| Restriction Sites | Please inquire |
| ACCN | NM_006258 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006258.1, NP_006249.1 |
| RefSeq Size | 3740 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 5592 |
| UniProt ID | Q13976 |
| Domains | cNMP, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, Long-term depression, Olfactory transduction, Vascular smooth muscle contraction |
| Gene Summary | Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (2) uses an alternate exon at its 5' end which result in the use of a distinct translation initiation site, compared to variant 1. This difference results in an isoform (2; also known as beta or 1beta) with a longer and distinct N-terminus, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220986 | PRKG1 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2 |
CNY 5,024.00 |
|
| RC220986L1 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, Myc-DDK-tagged |
CNY 7,424.00 |
|
| RC220986L2 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC220986L3 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220986L4 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG220986 | PRKG1 (tGFP-tagged) - Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2 |
CNY 6,624.00 |
