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RNA Polymerase II p14.5 (POLR2I) (NM_006233) Human Untagged Clone
CAT＃: SC116236
POLR2I (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I)
CNY 1,200.00
CNY 3,990.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hRPB14.5; RPB9 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006233, the custom clone sequence may differ by one or more nucleotides
ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGTAACAACATGC TGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGGAACTGTGATTACCAGCAGGA GGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCC GACGTGTCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGG CTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCC ACACTGCGGCCACCGCTGGACCGAGTGA >OriGene 5' read for NM_006233 unedited
GGGGTCAAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGAGGGCT GCGCGGGCTGCGCGTCGCCATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTAT TCGCTTCTGCCAGGAATGTGCCGGAACTGTGATTACCAGCAGGAGGCCGACAACAGCTGC ATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCCGACGTG TCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAG GAGGCTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTAC GTGTGCACAGCCCCACACTGCGGCCACCGCTGGACCGAGTGACCTCCTCTCTCCCCCGAG TGTAATAAACACCAGATTCCACANNCAAAAAAACCACCCCCCACTCAAAAAAAAAAACAA CTAACAAACCCCTCGACTCTAGATTGCGGCCGCGGCCATAGCTGTTTCCTGAACAGATCC CGGTGGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCC AGGGCCCACCATCCCTTGCCCTAATAAAATTAAATTGCATCATTTTGTCTGACTAGGTGC CCTTCTATAATATTATGGGGTGGAGGGGGGGGGGGAATGGTATTTATGCCCTGATTCTCA GAACCAAATACCATGACACCCTACCGCCAACACATACCCCATCAGAAAATATAGCACTTT ACGAACCACACTAAACCACAACGATACTTCAATAAAATATATTCCATATGAATTCCACAA CTCTATCCTCACACCGAGCCAACACACCACCCCCACCCTCAACCACCCACACCCACAATC CCTACTNAACAAACTCAAAANAACAACCCCAANAATC >OriGene 3' read for NM_006233 unedited
ACTTGTCCGCGGCACGCAATCTAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTGGGAAACCGGGGGTTTTTACACTCCGGGGGAAAAAAGAAG GCACTTCGTCCAACGGGGGCCCCAATGTGGGGCTTTGCCCACCTTTTAAAAGGGCATGGG GTCCTTTGCCCCCGCCCTTTGTGAATGGAAAAAACAACCCCCTTTTGGCCCCACTTTTTG CACGGGTGGGCCTCGGTCCCCGGGAACCTGGGGGCCTGGGACACCTTGGGGATAATTTGG GGCAATTCCTCCACTTTGGGCCGGAACTTTTTGACATAAATGCAACTGTTGTCGGCCCCC TTCTGGTAATCACAATTCCCGGCCATTTCTGGCAAAAACGAATTCCCCCCAAACCCCGCT CTTAAATCCCCTCCGGCTCCCTTGGGAACCCCAATCCCCCCATACCTTCTGGTGCCCAAT TTCCCGGCGCCCCTATAAGAGACCCATTAACAAAATCTGAACGGTACCTTAAAAAACCCT TCCTTTTTTAAACCTCCACCCGTCACCCTTTACCCCTTATTTTTGTTCCCCGCCCGGGTT TTTACCAACCTTTGAAAACATCCCTTTTTTTCGGCCCCAAAACAACCTCCCCACTCCTCA AATGGGGGCGCAATTTCAAAACCCTCGAGTCAAACCCCCCTTTCCCCTCTCTTCGTTTCT TCTCCAAACCCACTACACCCCCGCAAACACCCACCCCCCATACCATAAACACCTTTAATT AAAAAATCTCCCCCATATCTCCCTCCATATCTTTATCCCACACCGTTCATCCTTCTTTTT ATATTTTTTGCAGTGGTATTTTCCCTACACCACCTCTTCATTCCCCTCCTCACCTCTTAT CTCTCTCCCCCACCCCATCTTCCACCCCCACTACTTCCCTTCATGCCTCTCTCTCTCCAC CTCTCCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006233 |
| Insert Size | 450 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006233.4, NP_006224.1 |
| RefSeq Size | 885 bp |
| RefSeq ORF | 378 bp |
| Locus ID | 5438 |
| UniProt ID | P36954 |
| Domains | TFIIS, RNA_POL_M_15KD |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| Gene Summary | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A new p65 isoform that bind the glucocorticoid hormone and is expressed in inflammation liver diseases and COVID-19
,null,
Scientific Reports
,PubMed ID 34824310
[POLR2I]
|
|
Mrgprs on vagal sensory neurons contribute to bronchoconstriction and airway hyperresponsiveness
,null,
Nature neuroscience
,PubMed ID 29403029
[POLR2I]
|
|
Expression of UDP-Glucuronosyltransferase 1 (UGT1) and Glucuronidation Activity toward Endogenous Substances in Humanized UGT1
,null,
Drug Metabolism and Disposition
,PubMed ID 25953521
[POLR2I]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209776 | POLR2I (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I) |
CNY 1,200.00 |
|
| RC209776L3 | Lenti-ORF clone of POLR2I (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I) |
CNY 5,890.00 |
|
| RC209776L4 | Lenti-ORF clone of POLR2I (mGFP-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I) |
CNY 5,890.00 |
|
| RG209776 | POLR2I (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I) |
CNY 4,370.00 |
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