HEF1 (NEDD9) (NM_006403) Human Untagged Clone
CAT#: SC116092
NEDD9 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1
CNY 6,120.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CAS-L; CAS2; CASL; CASS2; HEF1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC116092 sequence for NM_006403 edited (data generated by NextGen Sequencing)
ATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAG GAACTGGCCTTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTG GAAGGATGGTGGCTGTGCTCGTTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTG AAGCTTCTGATTGGTCCCATGCAGGAGACTGCCTCCAGTCACGAGCAGCCTGCCTCTGGA CTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAGTGCCAAACCCACAGGCTGCT CCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAATTTACCAAGTC CCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTGCAGAGA AGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCCCGTGAGGACA GGCCATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGATGTCTATGATATCCCT CCTTCTCATACCACTCAAGGGGTATACGACATCCCTCCCTCATCAGCAAAAGGCCCTGTG TTTTCAGTTCCAGTGGGAGAGATAAAACCTCAAGGGGTGTATGACATCCCGCCTACAAAA GGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAGCAGGGCTTAGGGAAAAAGAC TATGACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACCGGAGGGGGTT TATGACATTCCTCCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAAC TGTGACATTCCAGGAGCTGCAGAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCGAAT CACCCACCCCCGCAACTCGGACAGTCAGTGGGCTCTCAGAACGACGCATATGATGTCCCC CGAGGCGTTCAGTTTCTTGAGCCACCAGCAGAAACCAGTGAGAAAGCAAACCCCCAGGAA AGGGATGGTGTTTATGATGTCCCTCTGCATAACCCGCCAGATGCTAAAGGCTCTCGGGAC TTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAGGCAGCACCCGGAGTAACATG TCCACGTCTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGCTCAGGACAAA AGGCTCTTCCTGGATCCAGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTT GAGATGGGTGTCTCCAGCCTAATGGCACTGGTCACTACCGACTGGCGGTGTTACGGATAT ATGGAAAGACACATCAATGAAATACGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAG GAGTACCTCCACTTTGTCAAGGGAGCTGTTGCAAATGCTGCCTGCCTCCCGGAACTCATC CTCCACAACAAGATGAAGCGGGAGCTGCAACGAGTCGAAGACTCCCACCAGATCCTGAGT CAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGAATATCTTGGCCATCAACAAG CCCCAGAACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGACGGTGCCCGAT GACGCCAAGCAGCTCACCACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGC CCTGGCAGCTTGCATCTGAAGAATGGGCCGGAGAGCATCATGAACTCAACGGAGTACCCA CACGGTGGCTCCCAGGGACAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCCCACAAC AAGGCACTGCCCCCAGGCCTGAGCAAGGAGCAGGCCCCTGACTGTAGCAGCAGTGATGGT TCTGAGAGGAGCTGGATGGATGACTACGATTACGTCCACCTACAGGGTAAGGAGGAGTTT GAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCATGAAACAGAACAAGATGCAG CTGGAACATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTACAAAGCCCGTG GAGAATGACATCTCGAAGTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTG AGTGCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGACCAATGTGAGACCCATTTCATT TCCCTTCTCAACGCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCCCAGCCCCCGCGA ATCTTCGTGGCACACAGCAAGTTTGTCATCCTCAGTGCACACAAACTGGTGTTCATTGGA GACACGCTGACACGGCAGGTGACTGCCCAGGACATTCGCAACAAAGTCATGAACTCCAGC AACCAGCTCTGCGAGCAGCTCAAGACTATAGTCATGGCAACCAAGATGGCCGCCCTCCAT TACCCCAGCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGACCTTTCTAGAAAT GCCCAGCTGTTCAAGCGCTCTTTGCTGGAGATGGCAACGTTCTGA Clone variation with respect to NM_006403.3 141 a=>g;525 c=>t;1476 t=>c;2367 c=>t >OriGene 5' read for NM_006403 unedited
TAGCTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCTGCGACTG ACAAGCGGCTCTGCCCGGGACCTTCTCGCTTTCATCTAGCGCTGCACTCAATGGAGGGGC GGGCACCGCAGTGCTTAATGCTGTCTTAACTAGTGTAGGAAAACGGCTCAACCCACCGCT GCCGAAATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGT GCCGAGGAACTGGCCTTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGG GGACTGGAAGGATGGTGGCTGTGCTCGTTACACGGTCGGCAAGGCATTGTCCCAGGCAAC CGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACTGCCTCCAGTCACGAGCAGCCTGCC TCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAGTGCCAAACCCACAG GCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAATTTAC CAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTG CAGAGAAGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCNCGTG AGGACAGGCCATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGATGTCTATGAT ATCCCTCCTTCTCATACCACTCAAGGGGTATACGACATCCCTNCCTCATCAGCAAAGGCC CTGTGTTTTCAGTTCCAGTGGGGAGAGAAAAACCTCANGGGGGTGTATGACATNCCCGCC TACAAAGGGTATATGCCATTCCCGCCCTCTGCTGCCGGATGAAGCAGGNCTTAGGGAAAA GACTATGACTTCCCCCTCCATGAGAAGNCTGNAAGGCGACTCACCCGNGGGGGGGGTTTA GCT >OriGene 3' read for NM_006403 unedited
AGCTCTGGACCGGCGGCCGCATNCTAGNAGTCGGTTTTTTTTTTTTTTTTTTCAGAGACT AAAATTTAATGATTTAAAAATAGCCTCTACATACAAGATGGCAAAGAGTAATACAGATTG AACATTTGGTTTTAAAAATGTGAAATGTCTCCACTTAGCGTAGATCAATCAAGTCAGCCA TCTCCTAAGAAATACACATTATACAATGAAATCTACAAAGACACACTTTTTAACTTCAAG CGTTGTTGATTTTCAGCAACCCTCTTCCCACATGAACATTTCCTTGTAATGTAATGTATG ACTTTTAATCTTCTTTTGGCAGAGTAGGACTTTGAGAATTATAATAGCAGTTGTTTTGAA AAGCACCTTCTATGATCATTTATTTCCACTGATTTTTTTTTTAATCACGCTGAGATCATG CATTCTTGTTTTTTAAATCCTTTTCTGTTCCAATGATTTGGTTGTTTAAAACAAGCAAGC TTAATTCAGTCAGGCATATATTGCAAATAAAAACATGTTTCCAAGTAGGCTGGAATAAAA ATGCTAAATAAGAACTGGGCCAAAAGATCATAGATAATGTGCTTCTTCTCTTGGTAATAT GTAAATACATTTACCCATGGGGNTAGAAGTATTTCACCTGCCCCATGAAGAACCTGATGG CTTGTGGNNTGTCTGTGCTGTGTCAATACTAACAGATGTGAGGAGACAGACTATGGAATG GACGCTGTGACTATCTGTGAATGTGGGAAAGGGGGTTATACACACTGGCCACAGGAAAGT GCAAAATTAGATGGACTCTGTGTAGCTAGCCACTCTTGANTGTCAGGTCTGCATATGTGA GTTTTAAAGAAAGTGCAAGATAGCANTAAATTNCCTTTAGAGAGCAGGAATGTNCCTGGG AGCTCTATAGNTCCATGATCAAGGACCTGGCCAGTTTCCTTCAGTAATGGGGGGTAGTGC CACAAAGGGAATATTAGAGTCAAAAG |
Restriction Sites | NotI-NotI |
ACCN | NM_006403 |
Insert Size | 4310 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006403.2, NP_006394.1 |
RefSeq Size | 4532 bp |
RefSeq ORF | 2505 bp |
Locus ID | 4739 |
UniProt ID | Q14511 |
Domains | SH3 |
Gene Summary | The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (1) encodes isoform 1. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The Overexpression of Scaffolding Protein NEDD9 Promotes Migration and Invasion in Cervical Cancer via Tyrosine Phosphorylated FAK and SRC
,Sima, N;Cheng, X;Ye, F;Ma, D;Xie, X;L?, W;,
PLoS One. 2013 Sep 18;8(9):e74594
,PubMed ID 24058594
[NEDD9]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207200 | NEDD9 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1 |
CNY 6,112.00 |
|
RC207200L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, Myc-DDK-tagged |
CNY 8,512.00 |
|
RC207200L2 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
RC207200L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, Myc-DDK-tagged |
CNY 8,512.00 |
|
RC207200L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, mGFP tagged |
CNY 8,512.00 |
|
RG207200 | NEDD9 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1 |
CNY 7,712.00 |