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HEF1 (NEDD9) (NM_006403) Human Tagged ORF Clone
CAT＃: RC207200
NEDD9 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1
ORF Plasmid: tGFP
"NM_006403" in other vectors (6)
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CNY 6,112.00
CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAS-L; CAS2; CASL; CASS2; HEF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207200 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAGGAACTGGCCT TTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTGGAAGGATGGTGGCTGTGCTC GTTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACT GCCTCCAGTCACGAGCAGCCTGCCTCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAG TGCCAAACCCACAGGCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAAT TTACCAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTGCAGAGA AGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCCCGTGAGGACAGGCCATGGCT ACGTATACGAGTACCCATCCAGATACCAAAAGGACGTCTATGATATCCCTCCTTCTCATACCACTCAAGG GGTATACGACATCCCTCCCTCATCAGCAAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAGATAAAACCT CAAGGGGTGTATGACATCCCGCCTACAAAAGGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAG CAGGGCTTAGGGAAAAAGACTATGACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACC GGAGGGGGTTTATGACATTCCTCCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAAC TGTGACATTCCAGGAGCTGCAGAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCGAATCACCCACCCC CGCAACTCGGACAGTCAGTGGGCTCTCAGAACGACGCATATGATGTCCCCCGAGGCGTTCAGTTTCTTGA GCCACCAGCAGAAACCAGTGAGAAAGCAAACCCCCAGGAAAGGGATGGTGTTTATGATGTCCCTCTGCAT AACCCGCCAGATGCTAAAGGCTCTCGGGACTTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAG GCAGCACCCGGAGTAACATGTCCACGTCTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGC TCAGGACAAAAGGCTCTTCCTGGATCCAGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTT GAGATGGGTGTCTCCAGCCTAATGGCACTGGTCACTACCGACTGGCGGTGTTACGGATATATGGAAAGAC ACATCAATGAAATACGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAGGAGTACCTCCACTTTGTCAA GGGAGCTGTTGCAAATGCTGCCTGCCTCCCGGAACTCATCCTCCACAACAAGATGAAGCGGGAGCTGCAA CGAGTTGAAGACTCCCACCAGATCCTGAGTCAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGA ATATCTTGGCCATCAACAAGCCCCAGAACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGAC GGTGCCCGATGACGCCAAGCAGCTCACCACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGC CCTGGCAGCTTGCATCTGAAGAATGGGCCGGAGAGCATCATGAACTCAACGGAGTACCCACACGGTGGCT CCCAGGGACAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCCCACAACAAGGCACTGCCCCCAGGCCT GAGCAAGGAGCAGGCCCCTGACTGTAGCAGCAGTGATGGTTCTGAGAGGAGCTGGATGGATGACTACGAT TACGTCCACCTACAGGGTAAGGAGGAGTTTGAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCA TGAAACAGAACAAGATGCAGCTGGAACATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTAC AAAGCCCGTGGAGAATGACATCTCGAAGTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTG AGTGCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGACCAATGTGAGACCCATTTCATTTCCCTTCTCA ACGCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCCCAGCCCCCGCGAATCTTCGTGGCACACAGCAA GTTTGTCATCCTCAGTGCACACAAACTGGTGTTCATTGGAGACACGCTGACACGGCAGGTGACTGCCCAG GACATTCGCAACAAAGTCATGAACTCCAGCAACCAGCTCTGCGAGCAGCTCAAGACCATAGTCATGGCAA CCAAGATGGCCGCCCTCCATTACCCCAGCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGACCT TTCTAGAAATGCCCAGCTGTTCAAGCGCTCTTTGCTGGAGATGGCAACGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207200 protein sequence
Red=Cloning site Green=Tags(s) MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPMQET ASSHEQPASGLMQQTFGQQKLYQVPNPQAAPRDTIYQVPPSYQNQGIYQVPTGHGTQEQEVYQVPPSVQR SIGGTSGPHVGKKVITPVRTGHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPVFSVPVGEIKP QGVYDIPPTKGVYAIPPSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYN CDIPGAAEPVARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLH NPPDAKGSRDLVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQAL EMGVSSLMALVTTDWRCYGYMERHINEIRTAVDKVELFLKEYLHFVKGAVANAACLPELILHNKMKRELQ RVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTINTNAEALFRPG PGSLHLKNGPESIMNSTEYPHGGSQGQLLHPGDHKAQAHNKALPPGLSKEQAPDCSSSDGSERSWMDDYD YVHLQGKEEFERQQKELLEKENIMKQNKMQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNSGV SAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVTAQ DIRNKVMNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006403 |
| ORF Size | 2502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006403.2, NP_006394.1 |
| RefSeq Size | 4550 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 4739 |
| UniProt ID | Q14511 |
| Domains | SH3 |
| MW | 92.9 kDa |
| Gene Summary | The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
NEDD9 crucially regulates TGF-ß-triggered epithelial-mesenchymal transition and cell invasion in prostate cancer cells: Involvement in cancer progressiveness
,Morimoto, K;Tanaka, T;Nitta, Y;Ohnishi, K;Kawashima, H;Nakatani, T;,
Prostate 901-10 74 8. April 2014
,PubMed ID 24728978
[HEF1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207200L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, Myc-DDK-tagged |
CNY 8,512.00 |
|
| RC207200L2 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RC207200L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, Myc-DDK-tagged |
CNY 8,512.00 |
|
| RC207200L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1, mGFP tagged |
CNY 8,512.00 |
|
| RG207200 | NEDD9 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1 |
CNY 7,712.00 |
|
| SC116092 | NEDD9 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1 |
CNY 2,950.00 |
