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TRIM27 (NM_006510) Human Untagged Clone
CAT＃: SC116046
TRIM27 (untagged)-Human tripartite motif containing 27 (TRIM27)
CNY 3,832.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RFP; RNF76 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_006510 edited
GAATTCGGCACGAGGCCCCCGCAGGCCGGCGCCATGGCCTCCGGGAGTGTGGCCGAGTGC CTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTC GACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACT AACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGG CACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCC GGCGGCGAGATGGGCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAG GACCAGATGCCCATCTGCGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTG CTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCAT TTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCT GAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTG TATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTG GCCATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTC AGCAGCCTGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAG GACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACA CCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCCAAAAATGTCTATTCTTGACGGAG AGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGAAAATCCAAGAATTAAGA GAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTG ATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGAC AACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGG AGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAA GACTCAGTGTGCAGAAAAGGTGGAGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTG TCTTTGTGGTATGGGAAAGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTG CGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCCTTC TACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTGTGGGCCT GTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATC TGCCCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATG GAGACCTCCCCTTGAGGAGGTGAATTCAGGCCAAAAGGGCTGTTGGCTGTAATCCTACGC CAGGCACAAGGCATCTTGTTGCCTTGCCACGTCCTGTCACAGCTGGGTATCCTTACCATG TTCCACGCCCTTGCAGTGGGAGACAGGATGTCCATGTTCTCTACCATCCTTTTCCTTCCC ATGCAGATTGTGAAATGTAATGAGATGTATCAAGATATCCTAGAAATAAAAACCAGATGT CCACCTCCAGTGTTTCATACTTTCTGGTTTTACACATCGCTGGAGGGATAAAGAGTATGG ATAATCTTTGGATTTGGAGAGCCGTTCAAGATACTTCCAGCTTCTTGGCTCAGCCTGGCT TCCTCTGGTTCAGCCCCACATAATGATTATGGCTATTTGCTGTCATTTCTGGGCTAGGGC TCCTTTCTAACAACCTAGACTGGAATAAGGCCCTGTCAGCATGGCTCCCTTTATCCCAGT TTTCCGTCTGGGAACAGTACCTCTGCCCCTGATTCCCAATGTGCCATAGTTTTATTAACT CCATTAAAGAAGCCTGTATGTGTTTTGGTTAGTTACAGTTATTTTACAATAATGGTGGGT AATGGCCCCACCTCTGTTATGAGATAATGTTCTAATCAATGTCTCTGCCTTTGTATCTTT TCTGAGGGCTTTGTCTGTTCTCTTCATTCTAATGAAAGGTGTATTCTAGTGCTGGGTGCA TATCATCCAGGATAATATTCTGCCCAACTCCATCCTCTGTTACTAGATCCCTTACCAGTC ACATTTGTGGACTGGTGGCCAGTCGTATACCATCCCTGGAAGGATTCTGGGACAATATTC CAGGGATTCATTGACTTCTTGGCTCCTTTTCTCCATTTCCTTTGGGGGAAGGGGGAATTG ACCATGCTTAAGTGCATCCTATCAAGGGGCAGCTCCGTCCCCATGGCCATTGGATCATGA GACACTCTGAAGTCAGAAGGCTGGGGCAGATCACTTCAAGCAAGCCCCCATGATGGTTCT CAGTCCTGCTTCTCTGTGGGTACGTGCCCCTCTGTTTAAAAATAAACTGAATATGGATGT TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_006510 unedited
GGGTACTATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCCCCCGCA GGCCGGCGCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCC CGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTG CGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTCGTGCCCGCAGTGCCG GGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCACCTGGCCAACGTGACCCAACT GGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGA GAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGT GTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGA GGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAA GAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGAT GGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGA GTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCATCAATGG TGCCATCACCCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCGCTCACCTAG AAGAGAAGCAGCACCAGCCCACCCAGGAGCTCCTGCCAGACCATTGGGGACACATTGAGC AGGGCTGAAAGGATACGGATCCTGAACCTTGAATACACCTCCAGATTGCAAGAGAAATAC CCATTTTGCCC >OriGene 3' read for NM_006510 unedited
TTTTAAAAANNCCTCTGTGNACCGCGCCGCATTCTANGATCGATNTTTTTTTTTTTTTTT TTTTTTTTTTTTTAAACATCCATATTCAGTTTATTTTTAAACAGAGGGGCACGTACCCAC AGAGAAGCAGGACTGAAAACCATCATGGGGGCTTGCTTGAAGTGATCTGCCCCAGCCTTC TGACTTCAAAGTGTCTCATGATCCAATGGCCATGGGGACGGAGCTGCCCCTTGATAGGAT GCACTTAAGCATGGTCAATTCCCCCTTCCCCCAAAGGAAATGGAGAAAAGGAGCCAAGAA GTCAATGAATCCCCTGGAATATTGTCCCAGAATCCTTCCAGGGATGGTATACGACTGGCC ACCAGTCCACAAATGTGACTGGGTAGGGATCTAGTAACAGAGGATGGAGTTGGGCAGAAT ATTATCCTGGATGATATGCACCCAGCACTAGAATACACCTTTCATTAGAATGAAGAGAAC GACAAAGCCCTCAAAAAAGATACAAAAGCAAAGACATTGATTAGAACATTATCTCATAAC AGAAGTTGGGGCCATTACCCACCATTATTGGAAAATACCTGGAAACTAACCAAAACCCTT ACAAGGCTTCTTTTNTGGGAGTGTAATAAAACTATTGGCCCATTGGNGAAATCAGGGGCC AAAAGTACTTGTTCCCCNACCGGAAAACCTGGGATAAAGGGGAACCCTTCCTGAAAGGGC CCTTATTCTCAGTCTTAGGGTTGTTTAAAGAGAGACCCTACCCCCANAAATGCACCCCAA ATATCCTTTATTCCTTATGTGGGGCGCTGAACCCAAGAGAAACCACGGTTTACCCCAAAA ACCTGGAATTTTCTTAAACGGCCTCTCACAACCCAAAGATTTTCCATCCTTTTTATTCCC TTCCCCGATGGTGTAAACCCCAAAAATTGAAAACCTGGGGGGGGGCCACTGGGGTTTTTA TTTGAGAGATTTGGGACCCTTTTTTAACTTTAACAATTGGGGGGGAAGAGAAAGAGGGGG NAAAAAATTGGCCTTTCTTCCCCCTTGAGAGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006510 |
| Insert Size | 2770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006510.3, NP_006501.1 |
| RefSeq Size | 2984 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 5987 |
| UniProt ID | P14373 |
| Domains | zf-B_box, RING, SPRY, PRY |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (alpha) lacks 280 nt in the coding region, resulting in a frameshift and use of an upstream stop codon. It encodes isoform alpha which has a distinct C-terminus as compared to isoform beta. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202184 | TRIM27 (Myc-DDK-tagged)-Human tripartite motif containing 27 (TRIM27) |
CNY 3,824.00 |
|
| RC202184L1 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), Myc-DDK-tagged |
CNY 6,224.00 |
|
| RC202184L2 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), mGFP tagged |
CNY 5,890.00 |
|
| RC202184L3 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202184L4 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), mGFP tagged |
CNY 5,890.00 |
|
| RG202184 | TRIM27 (tGFP-tagged) - Human tripartite motif containing 27 (TRIM27) |
CNY 5,424.00 |
