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TRIM27 (NM_006510) Human Tagged ORF Clone
CAT＃: RG202184
- TrueORF®
TRIM27 (tGFP-tagged) - Human tripartite motif containing 27 (TRIM27)
ORF Plasmid: DDK
"NM_006510" in other vectors (6)
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CNY 5,424.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RFP; RNF76 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202184 representing NM_006510
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCG CAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGC AGAGACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCAC CTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGG GCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGT GTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAG GAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGG GGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGA GCAGCTGTATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCC ATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCG CTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAGGACATTGGGGACACATTGAGCAG GGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTT GCCCAAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGA AAATCCAAGAATTAAGAGAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCC CAGCCTGATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGACAAC CCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGGAGACATTATTGGG AGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGG AGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAAAGAATATTGGGCTCTT ACCTCCCCAATGACTGCCCTACCCCTGCGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATG CTGGTGAGGTCTCCTTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTG TGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATCTGC CCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202184 representing NM_006510
Red=Cloning site Green=Tags(s) MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRH LANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFK EQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLA IYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIF AQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDN PERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWAL TSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIIC PMSGIDGFSGHVGNHGHSMETSP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006510 |
| ORF Size | 1539 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006510.5 |
| RefSeq Size | 2984 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 5987 |
| UniProt ID | P14373 |
| Domains | zf-B_box, RING, SPRY, PRY |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202184 | TRIM27 (Myc-DDK-tagged)-Human tripartite motif containing 27 (TRIM27) |
CNY 3,824.00 |
|
| RC202184L1 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), Myc-DDK-tagged |
CNY 6,224.00 |
|
| RC202184L2 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), mGFP tagged |
CNY 5,890.00 |
|
| RC202184L3 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202184L4 | Lenti ORF clone of Human tripartite motif containing 27 (TRIM27), mGFP tagged |
CNY 5,890.00 |
|
| SC116046 | TRIM27 (untagged)-Human tripartite motif containing 27 (TRIM27) |
CNY 3,832.00 |
