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RRAGA (NM_006570) Human Untagged Clone
CAT＃: SC116000
RRAGA (untagged)-Human Ras-related GTP binding A (RRAGA)
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FIP-1; FIP1; RAGA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116000 sequence for NM_006570 edited (data generated by NextGen Sequencing)
ATGCCAAATACAGCCATGAAGAAAAAGGTGCTGCTGATGGGGAAGAGCGGGTCGGGGAAG ACCAGCATGAGGTCGATAATCTTCGCCAATTACATTGCTCGCGACACCCGGCGCCTGGGG GCCACCATTGACGTGGAACACTCCCACGTCCGATTCCTAGGGAACCTGGTGCTGAACCTG TGGGACTGTGGCGGTCAGGACACCTTCATGGAAAATTACTTCACCAGCCAGCGAGACAAT ATCTTCCGTAACGTGGAAGTTTTGATTTACGTGTTTGACGTGGAGAGCCGCGAACTGGAA AAGGACATGCATTATTACCAGTCGTGTCTGGAGGCCATCCTCCAGAACTCTCCTGACGCC AAAATCTTCTGCCTGGTGCACAAAATGGATCTGGTTCAGGAGGATCAGCGTGACCTGATT TTTAAAGAGCGAGAGGAAGACCTGAGGCGTCTGTCTCGCCCGCTGGAGTGTGCTTGTTTT CGAACGTCCATCTGGGATGAGACGCTCTACAAAGCCTGGTCCAGCATCGTCTACCAGCTG ATTCCCAACGTTCAGCAGCTGGAGATGAACCTCAGGAATTTTGCCCAAATCATTGAGGCC GATGAAGTTCTGCTGTTCGAAAGAGCTACATTCTTGGTTATTTCCCACTACCAGTGCAAA GAGCAGCGCGACGTCCACCGGTTTGAGAAGATCAGCAACATCATCAAACAGTTCAAGCTG AGCTGCAGTAAATTGGCCGCTTCCTTCCAGAGCATGGAAGTTAGGAATTCCAACTTCGCT GCTTTCATCGACATCTTCACCTCAAATACGTACGTGATGGTGGTCATGTCAGATCCGTCG ATCCCTTCTGCGGCCACTCTGATCAACATTCGCAATGCCCGGAAACACTTTGAGAAGCTG GAGAGAGTGGATGGCCCCAAGCACAGTCTCCTTATGCGTTGA Clone variation with respect to NM_006570.4 >OriGene 5' read for NM_006570 unedited
GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCCCCGGCGGGTGATGCCAAAT ACAGCCATGAAGAAAAGGTGCTGCTGATGGGGAAGAGCGGGTCGGGGAAGACCAGCATGA GGTCGATAATCTTCGCCAATTACATTGCTCGCGACACCCGGCGCCTGGGGGCCACCATTG ACGTGGAACACTCCCACGTCCGATTCCTAGGGAACCTGGTGCTGAACCTGTGGGACTGTG GCGGTCAGGACACCTTCATGGAAAATTACTTCACCAGCCAGCGAGACAATATCTTCCGTA ACGTGGAAGTTTTGATTTACGTGTTTGACGTGGAGAGCCGCGAACTGGAAAAGGACATGC ATTATTACCAGTCGTGTCTGGAGGCCATCCTCCAGAACTCTCCTGACGCCAAAATCTTCT GCCTGGTGCACAAAATGGATCTGGTTCAGGAGGATCAGCGTGACCTGATTTTTAAAGAGC GAGAGGAAGACCTGAGGCGTCTGTCTCGCCCGCTGGAGTGTGCTTGTTTTCGAACGTCCA TCTGGGATGAGACGCTCTACAAGCCTGGTCCAGCATCGTCTACCAGCTGATTCCCAACGT TCAGCAGCTGGAGATGAACCTCANGAATTTTTGCCCAAATCATTGAGGCCGATGAAAGTC TGCTGNTCGAAAGAGCTACATTCTTGGTTATTTCCCACTACCAGTGCAAGAGCAGCGCGA CGTCCACCGGTTGAGAAGATCAGCACTCATCAAACGTTCAAGCTGAGCTGCAGTAATGGC CCGGTTTCTTCCAGACATGGGAGTTAGGAATTCCAACTCGCTGCTTCATCGACATCTCAC TNAAATACTCGGGATGGGGGGCATGTAAATCCGGNGATCCTTTTGGGGGCCTTTGATCAC ATTGCAATGCCGGAACCTTTTAGAGCTGGAAAAGGGATGGCCCACCCAAGTTCCTTAGGG TGAAATGGCCAAGCTT >OriGene 3' read for NM_006570 unedited
GCAACCTTCCAGGGCCAGAAGAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGGGCTAACCCGTTTTTT CATGAAGGAACCCAAAAAAAATTTATAAAGGCATTTTAATACCTTGGGGAAAAAAAAAAA ACATTCCGTTTTTGCAATCTGATCCGGAATACAGGAAAATCCTGGGAAAGGCTTTGAGGG GCATATTTATCATAACCGGGGAAGGAAAACCCCCCCGACCCCCCTACTTTAGGGGTACCC CATACCAACTTTTAAAAAAGGGCAGCTTCAAGCTCTACTGGGTAGCTGAAAAAATAGCGN NNAATGTTTAAAAACTGGGGAAGCGGGGAAAAAAATGAAAGGGGTAAGCCGCCCACCCTT CAAAGCCCACTTTTAAGCCCCCGACTTTTTGAATATTAAAAATAAGGGGGGCAAACAAAA AAGGCAATTCAGCATTTTCAAAAAGAGCATTTGGCAATATTCAACCCATAAGGAAACTGG GCTTGGGGCCATCCACTCTCTCCAGCTTTCAAAGGGTTTCCGGGCATTGCGAATGTTTAT CANAGGGGCCGCAAAAGGGATCGACGGATCTGACATGACCACCATCCCGTACGTATTTGA GGTGAAGATGTCGATTGGGGNNATGAAGGGGAATTCCTAACTTCCATGCTTGGAGGGAAC GGCCAATTACCTGGACCCACCTGGAACGGTTGGAAGAAGGGGCGGATCTTTCAAACGGGG GGACCTCGGCTGCTTTTTGCCTGGAGGGGAGAAAACCAAAGAGGGCTTTTCCAACAGAAA ACTTCTTGGCTTAGGATTGGGCAAATCCGGAGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006570 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006570.4, NP_006561.1 |
| RefSeq Size | 1657 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 10670 |
| UniProt ID | Q7L523 |
| Domains | Gtr1_RagA |
| Gene Summary | Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Amino acids activate mTOR complex1 without changing Rag GTPase guanyl nucleotide charging
,Oshiro, N;Rapley, J;Avruch, J;,
J. Biol. Chem. Dec 2013.
,PubMed ID 24337580
[RRAGA]
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