DDX18 (NM_006773) Human Untagged Clone
CAT#: SC115885
DDX18 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18)
CNY 4,912.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | Has1; MrDb |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115885 sequence for NM_006773 edited (data generated by NextGen Sequencing)
ATGTCACACCTGCCGATGAAACTCCTGCGTAAGAAGATCGAGAAGCGGAACCTCAAATTG CGGCAGCGGAACCTAAAGTTTCAGGGGGCCTCAAATCTGACCCTATCGGAAACTCAAAAT GGAGATGTATCTGAAGAAACAATGGGAAGTAGAAAGGTTAAAAAATCAAAACAAAAGCCC ATGAATGTGGGCTTATCAGAAACTCAAAATGGAGGCATGTCTCAAGAAGCAGTGGGAAAT ATAAAAGTTACAAAGTCTCCCCAGAAATCCACTGTATTAACCAATGGAGAAGCAGCAATG CAGTCTTCCAATTCAGAATCAAAAAAGAAAAAGAAGAAAAAGAGAAAAATGGTGAATGAT GCTGAGCCTGATACGAAAAAAGCAAAAACTGAAAACAAAGGGAAATCTGAAGAAGAAAGT GCCGAGACTACTAAAGAAACAGAAAATAATGTGGAGAAGCCAGATAATGATGAAGATGAG AGTGAGGTGCCCAGTCTGCCCCTGGGACTGACAGGAGCTTTTGAGGATACTTCGTTTGCT TCTCTATGTAATCTTGTCAATGAAAACACTCTGAAGGCAATAAAAGAAATGGGTTTTACA AACATGACTGAAATTCAGCATAAAAGTATCAGACCACTTCTGGAAGGCAGGGATCTTCTA GCAGCTGCAAAAACAGGCAGTGGTAAAACCCTGGCTTTTCTCATCCCTGCAGTTGAACTC ATTGTTAAGTTAAGGTTCATGCCCAGGAATGGAACAGGAGTCCTTATTCTCTCACCTACT AGAGAACTAGCCATGCAAACCTTTGGTGTTCTTAAGGAGCTGATGACTCACCACGTGCAT ACCTATGGCTTGATAATGGGTGGCAGTAACAGATCTGCTGAAGCACAGAAACTTGGTAAT GGGATCAACATCATTGTGGCCACACCAGGCCGTCTGCTGGACCATATGCAGAATACCCCA GGATTTATGTATAAAAACCTGCAGTGTCTGGTTATTGATGAAGCTGATCGTATCTTGGAT GTGGGGTTTGAAGAGGAATTAAAGCAAATTATTAAACTTTTGCCAACACGTAGACAGACT ATGCTCTTTTCTGCCACCCAAACTCGAAAAGTTGAAGACCTGGCAAGGATTTCTCTGAAA AAGGAGCCATTGTATGTTGGCGTTGATGATGATAAAGCGAATGCAACAGTGGATGGTCTT GAACAGGGATATGTTGTTTGTCCTTCTGAAAAGAGATTCCTTCTGCTCTTTACATTCCTT AAGAAGAACCGAAAGAAGAAGCTTATGGTCTTCTTTTCATCTTGTATGTCTGTGAAATAC CACTATGAGTTGCTGAACTACATTGATTTGCCCGTCTTGGCCATTCATGGAAAGCAAAAG CAAAATAAGCGTACAACCACATTCTTCCAGTTCTGCAATGCAGATTCGGGAACACTATTG TGTACGGATGTGGCAGCGAGAGGACTAGACATTCCTGAAGTCGACTGGATTGTTCAGTAT GACCCTCCGGATGACCCTAAGGAATATATTCATCGTGTGGGTAGAACAGCCAGAGGCCTA AATGGGAGAGGGCATGCCTTGCTCATTTTGCGCCCAGAAGAATTGGGTTTTCTTCGTTAC TTGAAACAATCCAAGGTTCCATTAAGTGAATTTGACTTTTCCTGGTCTAAAATTTCTGAC ATTCAGTCTCAGCTTGAGAAATTGATTGAAAAGAATTACTTTCTTCATAAGTCAGCCCAG GAAGCATATAAGTCATACATACGAGCCTATGATTCCCATTCTCTGAAACAGATCTTTAAT GTTAATAACCTAAATTTGCCTCAGGTTGCTCTGTCATTTGGTTTCAAGGTGCCTCCCTTC GTTGATCTGAACGTCAACAGTAATGAAGGCAAGCAGAAAAAGCGAGGAGGTGGTGGTGGA TTTGGCTACCAGAAAACCAAGAAAGTTGAGAAATCCAAAATCTTTAAACACATTAGCAAG AAATCATCTGACAGCAGGCAGTTCTCTCACTGA Clone variation with respect to NM_006773.3 1617 c=>t >OriGene 5' read for NM_006773 unedited
GTTACCGTACANAAATTTGTTAATACGAACTCACTATAGGGNCGGCCCGCGTAATTCGGC ACGAGGGTTACTGTGTTGGCGCACTTATTCTAGGCACTTTGTTGGGCAGAATGTCACACC TGCCGATGAAACTCCTGCGTAAGAAGCTCGAGAAGCGGAACCTCAAATTGCGGCAGCGGA ACCTAAAGTTTCAGGGGGCCTCAAATCTGACCCTATCGGAAACTCAAAATGGAGATGTAT CTGAAGAAAAATGGGAAGTAGAAAGGTTAAAAAATCAAAACAAAAGCCCATGAATGTGGG CTTATCAGAAACTCAAAATGGAGGCATGTCTTAAGAAGCAGTGGGAAATATTAAAAGTTA CAAAGTCTCCCCAGAAATCCACTTGTATTAACCAATGGAGAAGCAGCAATGCAGTCTTCC AATTCAGAATCAAAAAAGAAAAAGAAGAAAAAGAGAAAAATGGTGAATGATGCTGAGCCT GATACGAAAAAAGCAAAAACTGAAAACAAAGGGAAATCTGAAGAAGAAAGTGCCGAGACT ACTAAAGAAACAGAAAATAATGTGGAGAAGCCAGATAATGATGAAGATGAGAGTTGAGGT GCCCAGTCTGCCCCTGGGACTGACAGGAGCTTTTGAGGATACTNTCGTTTGCTTCTCTAT GTAATCTTGTCAATGAAAACACTCTGAAGGCAATAAAAGAAATGGGTTTTACAAACATGA CTGANATTCAGCATAAAAGTATCAGACCACTTTCTGNANNGCANGGATCTTCTAGCAGCT GCAAAAACANGCAGTGGTTAAACCCTGGCTTTTCTCATCCCTGCAGTTGAACTCATTGTT AAGTTAANGTTTCATGCCCAGATGGAACAGGAGTCCTTATTCTCTCACTACTAGAGACTA GCCTGCAACCTTTTTGTGTTCTTAAG >OriGene 3' read for NM_006773 unedited
NGGGGGGAGGTAACGCTATGGAACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTT TTTTTTTTTCAACATTGAAGTAATTATTTAATGACACAGGGAAAATAACATGTTATGAAA ACAAGCTGGTTACAAGTAGTAGGTAGATGACTTAATTTTTGATAAAAAAATTAAAAAGCA TAAACATGCATATAAAAATTAGATTATGTACAAAATACCAACAGTATTTACTTCTGCTCA GTAATTAAATATTCTTCCCTTTGTTTTTGTCTTTTAAAAAACATTATTTCTGAAAAAAAA AATCAGAAAAACATGATCGTGGAGAGAATTATTAAACACCAGGAATTAGGAACCCAAGAA GCTAAGCCAGGAGCTCAGCAGTCAAGGTTGGAAGTCCAGGACAGGTCCGACCAGGGTGAT GGTGGCCAAGCCCAGAAATGGAAGATTGTTCTTTTCTTTTCCTACTGGAATGTTCTTTAC CAGTTTTCTCTATATTCGATGTTAGCACACTTATTTAATGGGATCCTATGATTAACTGGC ACAACAGCAAAATGATGCTAGCAACTTTGGCTTTGAACTGCTACAAAATGTACTATCTTG TAGTTATATACTGTACAAAACACACTAGAAATCAAGTGCTCCTACAACGAAAAACTCATC TGACTAAATCACTGATTTTGCTGGTTTTTTTTCTAACTTTGTTCATTTTCAATCTTCACA GCAAATGTATTTTTTGTTTCAAGGCCTGGAAGAGGTATTTTTTTCTCAAAAAGGAGCTAA CATTAATGTGGTGCGGGGCACCGTCTGAAAGCTTGGCTTTAACTCCAAGTTAATTTGGCC TTGCCTTTAAGGTCTCTAAAAAGGGCAAAGCTA |
Restriction Sites | ECoRI-NOT |
ACCN | NM_006773 |
Insert Size | 4000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006773.3, NP_006764.3 |
RefSeq Size | 3790 bp |
RefSeq ORF | 2013 bp |
Locus ID | 8886 |
UniProt ID | Q9NVP1 |
Domains | DEAD, helicase_C |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells
,Redmond, AM;Byrne, C;Bane, FT;Brown, GD;Tibbitts, P;O'Brien, K;Hill, AD;Carroll, JS;Young, LS;,
Oncogene
,PubMed ID 25284587
[DDX18]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205768 | DDX18 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18) |
CNY 4,912.00 |
|
RC205768L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18), Myc-DDK-tagged |
CNY 7,312.00 |
|
RC205768L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18), mGFP tagged |
CNY 7,700.00 |
|
RC205768L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC205768L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18), mGFP tagged |
CNY 7,312.00 |
|
RG205768 | DDX18 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18) |
CNY 6,512.00 |
|
SC323819 | DDX18 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18) |
CNY 4,912.00 |