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TMED2 (NM_006815) Human Untagged Clone
CAT＃: SC115869
TMED2 (untagged)-Human transmembrane emp24 domain trafficking protein 2 (TMED2)
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | p24; P24A; p24b1; p24beta1; RNP24 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115869 sequence for NM_006815 edited (data generated by NextGen Sequencing)
ATGGTGACGCTTGCTGAACTGCTGGTGCTCCTGGCCGCTCTCCTGGCCACGGTCTCGGGC TATTTCGTTAGCATCGACGCCCATGCTGAAGAGTGCTTCTTTGAGCGGGTCACCTCGGGC ACCAAGATGGGCCTCATCTTCGAGGTGGCGGAGGGCGGCTTCCTGGACATCGACGTGGAG ATTACAGGACCAGATAACAAAGGAATTTACAAAGGAGACAGAGAATCCAGTGGGAAATAC ACATTTGCTGCTCACATGGATGGAACATACAAATTTTGTTTTAGTAACCGGATGTCCACC ATGACTCCAAAAATAGTGATGTTCACCATTGATATTGGGGAGGCTCCAAAAGGACAAGAT ATGGAAACAGAAGCTCACCAGAACAAGCTAGAAGAAATGATCAATGAGCTAGCAGTGGCG ATGACAGCTGTAAAGCACGAACAGGAATACATGGAAGTCCGGGAGAGAATACACAGAGCC ATCAACGACAACACAAACAGCAGAGTGGTCCTTTGGTCCTTCTTTGAAGCTCTTGTTCTA GTTGCCATGACATTGGGACAGATCTACTACCTGAAGAGATTTTTTGAAGTCCGGAGAGTT GTTTAA Clone variation with respect to NM_006815.3 30 t=>c >OriGene 5' read for NM_006815 unedited
GTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCGGCGGCGGCGGCGGCGGC TGTGGAGGCCGCAGTCCGGGTCCTGGCTTCGGCCTCAGCCCCACCATGGTGACGCTTGCT GAACTGCTGGTGCTCCTGGCCGCTCTCCTGGCCACGGTCTCGGGCTATTTCGTTAGCATC GACGCCCATGCTGAAGAGTGCTTCTTTGAGCGGGTCACCTCGGGCACCAAGATGGGCCTC ATCTTCGAGGTGGCGGAGGGCGGCTTCCTGGACATCGACGTGGAGATTACAGGACCAGAT AACAAAGGAATTTACAAAGGAGACAGAGAATCCAGTGGGAAATACACATTTGCTGCTCAC ATGGATGGAACATACAAATTTTGTTTTAGTAACCGGATGTCCACCATGACTCCAAAAATA GTGATGTTCACCATTGATATTGGGGAGGCTCCAAAAGGACAAGATATGGAAACAGAAGCT CACCAGAACAAGCTAGAAGAAATGATCAATGAGCTAGCAGTGGCGATGACAGCTGTAAAG CACGAACAGGAATACATGGAAGTCCGGGAGAGAATACACAGAGCCATCAACGACAACACA AACAGCAGAGTGGTCCTTTGGTCCTTCTTTGAAGCTCTTGTTCTAGTTGCCATGACATTG GGACAGATCTACTACCTGAAGAGATTTTTTGAAGTCCGGAGAGTTGTTTAAAAAGCCTCT TCCTGATGATCCCAACTCAGAATTCACTGTTTACCANACACCTTGGTCATAATAATGTCA TTAGTTTCTCCATTTTNATTTTCTGAACTGTACATTCACAACTTATGTTTTCTTTGAGAT TAATAGATATTGGGGGGACAAACGCCTTTNTAGGAAAATTATAGTGAAAAATTGACAGNN TGATGGCATAATTTCTGGTTGATGGCTGCTCATTATATAGTCCTTCAGA >OriGene 3' read for NM_006815 unedited
TTCTCTCCCTTCCCCTCCCNCTACTATTCTCCTTGTGTTGACTTTGACACCGCGGCCGCA TCCTACGATCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAAAAAATGTTT ATTTACCGAACCTGGGGCATATTAGATACAACCAATTTTAAATTTACATCTTTTAAATCA GTTTTGAAGTGTTTCACACACACAAAAAACTTGGCATGCAACAGTTGTCCTAAGGTGAAA GTCACCTCATTAATAAACTGTTGCAAGTGTTCTCACCCAAGGGGGAAGTATGGTTTATCC TGAACATGAAAACCTGTAACAAATTTAAATTAATTATATAAAACTCGTTACTTGTAGTTT TGCCCTTGCAAAGATCCAAAAAAAAAAAAAAAAGCACAAGTAACTAATATGTATCAGTCA CAACATAATCCTGGATCATCCCCATACCAAAACCGGATGCTCTTTTAATTTTAAACCCAT CATCAGAGAACAAGAGAAAGTAATTTCATTTTACACAAAACAAGATTCACATGTGCCAAA AAAGAAATACCCAAAAGAAACAAAAACCAAAACCCTAATGCTGACACTGATGGTCAGTAC CCTGAACAGTGGCCCAAAGGCCACTGATCAAAAATAAAATAGTGGCTGTCTATCCATGAA ATGAAATCCAAGAAGCTTTAACCCCTTTGGCATTAGAAATCCAGATTTTTCCAATTATGT AAATGCTTATTTCTTACCTGCCCAAAGTGGAAGAAGTGAATGCGCCCAATGTGGAAAGAA AAACAGCCCCCCTTTCTTGGGGAAACCCACAAAATTGGGTTTTAACAGAACAATTAGGGG GTTAGGCCAAAAACCCAAAAATGGTTATTAACCTGCTTGGGTCATAAATTTAAGGGGGAG AAGGACTGGGCTCTAAAGGTACACAGGGAAAAAGTGTTGTGGGGAAAAATGTTGCCGCAG CCGAATTTTAAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006815 |
| Insert Size | 2090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006815.2, NP_006806.1 |
| RefSeq Size | 2060 bp |
| RefSeq ORF | 606 bp |
| Locus ID | 10959 |
| UniProt ID | Q15363 |
| Domains | EMP24_GP25L |
| Protein Families | Transmembrane |
| Gene Summary | Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The cargo receptor p24A facilitates calcium sensing receptor maturation and stabilization in the early secretory pathway
,null,
Biochemical and biophysical research communications
,PubMed ID 20361938
[TMED2]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206849 | TMED2 (Myc-DDK-tagged)-Human transmembrane emp24 domain trafficking protein 2 (TMED2) |
CNY 2,400.00 |
|
| RC206849L3 | Lenti ORF clone of Human transmembrane emp24 domain trafficking protein 2 (TMED2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206849L4 | Lenti ORF clone of Human transmembrane emp24 domain trafficking protein 2 (TMED2), mGFP tagged |
CNY 5,890.00 |
|
| RG206849 | TMED2 (tGFP-tagged) - Human transmembrane emp24 domain trafficking protein 2 (TMED2) |
CNY 4,000.00 |
