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Myotilin (MYOT) (NM_006790) Human Untagged Clone
CAT＃: SC115849
MYOT (untagged)-Human myotilin (MYOT), transcript variant 1
CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LGMD1; LGMD1A; MFM3; TTID; TTOD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115849 sequence for NM_006790 edited (data generated by NextGen Sequencing)
ATGTTTAACTACGAACGTCCAAAACACTTCATCCAGTCCCAAAACCCATGTGGCTCCAGA TTGCAGCCTCCTGGACCAGAAACCTCCAGCTTCTCTAGCCAGACCAAACAGTCTTCCATT ATCATCCAGCCCCGCCAGTGTACAGAGCAAAGATTTTCTGCCTCCTCAACACTGAGCTCT CACATCACCATGTCCTCCTCTGCTTTCCCTGCTTCTCCCCAGCAGCATGCTGGCTCCAAC CCAGGCCAAAGGGTTACAACCACCTATAACCAGTCCCCAGCCAGCTTCCTCAGCTCCATA TTACCATCACAGCCTGATTACAATAGCAGTAAAATCCCTTCCGCTATGGATTCCAACTAT CAACAGTCCTCAGCTGGCCAACCTATAAATGCAAAGCCATCCCAAACTGCAAATGCTAAG CCCATACCAAGAACTCCTGATCATGAAATACAAGGATCAAAAGAAGCTTTGATTCAAGAT TTGGAAAGAAAGCTGAAATGCAAGGACACCCTTCTTCATAATGGAAATCAACGTCTAACA TATGAAGAGAAGATGGCTCGCAGATTGCTAGGACCACAGAATGCAGCTGCTGTGTTTCAA GCTCAGGATGACAGTGGTGCACAAGACTCGCAGCAACACAACTCAGAACATGCGCGACTG CAAGTTCCTACATCACAAGTAAGAAGTAGATCAACCTCAAGGGGAGATGTGAATGATCAG GATGCAATCCAGGAGAAATTTTACCCACCACGTTTCATTCAAGTGCCAGAGAACATGTCG ATTGATGAAGGAAGATTCTGCAGAATGGACTTCAAAGTGAGTGGACTGCCAGCTCCTGAT GTGTCATGGTATCTAAATGGAAGAACAGTTCAATCAGATGATTTGCACAAAATGATAGTG TCTGAGAAGGGTCTTCATTCACTCATCTTTGAAGTAGTCAGAGCTTCAGATGCAGGGGCT TATGCATGTGTTGCCAAGAATAGAGCAGGAGAAGCCACCTTCACTGTGCAGCTGGATGTC CTTGCAAAAGAACATAAAAGAGCACCAATGTTTATCTACAAACCACAGAGCAAAAAAGTT TTAGAGGGAGATTCAGTGAAACTAGAATGCCAGATCTCGGCTATACCTCCACCAAAGCTT TTCTGGAAAAGAAATAATGAAATGGTACAATTCAACACTGACCGAATAAGCTTATATCAA GATAACACTGGAAGAGTTACTTTACTGATAAAAGATGTAAACAAGAAAGATGCTGGGTGG TATACTGTGTCAGCAGTTAATGAAGCTGGAGTGACTACATGTAACACAAGATTAGACGTT ACGGCACGTCCAAACCAAACTCTTCCAGCTCCTAAGCAGTTACGGGTTCGACCAACATTC AGCAAATATTTAGCACTTAATGGGAAAGGTTTGAATGTAAAACAAGCTTTTAACCCAGAA GGAGAATTTCAGCGTTTGGCAGCTCAATCTGGACTCTATGAAAGTGAAGAACTTTAA Clone variation with respect to NM_006790.2 >OriGene 5' read for NM_006790 unedited
TATAGGGCGGCCGCGAATTCGGCACGAGGATGCCTCTTCCTTCCCTTCAATAGTGGGTTA AACCCAGCTGGCACCCTCTGGAACTACGGGAACAATATTCTTCAAGAGAAGGTCACTCTA CCAAAGCCAGGAGCACAGTATTCTCAGGATCTCAACAAGGAAGAGCAGACCAAGGTTGCT TCTGATTCCTTACAACCTTCCGTAATTCCAGGCTTGTGGCCCCAAATTCAGGGCCCCACC CTTCCAGGAACAAATCATTATAGTNAATAATTTGGCCTTCATCCTTCCATATACCAACTA AGCATGTTTAACTACGAACGTCCAAAACACTTCATCCAGTCCCAAAACCCATGTGGCTCC AGATTGCAGCCTCCTGGACCAGAAACCTCCAGCTTCTCTAGCCAGACCAAACAGTCTTCC ATTATCATCCAGCCCCGCCAGTGTACAGAGCAAAGATTTTCTGCCTCCTCAACACTGAGC TCTCACATCACCATGTCCTCCTCTGCTNTCCCTGCTTCTCCCCAGCAGCATGCTGGCTCC AACCCAGGCCAAAGGGTTACAACCACCTATAACCAGTCCCCAGCCAGCTTCCTCAGCTCC ATATTACCATCACAGCCTGATTACANTAGCAGTAAAATCCCTTCCGCTATGGATTCCAAC TATCAACAGTCCTCAGCTGGCCAACCTANTAATGCANAGCCATCCCAAACTGCANATGCT AAAGCCATACCNAGAACTNCTGATCATGAAATACANGGATCANAAGAAGCTNTGATTNCA GATTTGGAAAGAAAGCTGNAATGCANGGACACCCTTNCTTCATATGGANNATCACGTCTA CATATGAAGAGAAGATGGCTCGCANATTGCTAGGACACAGAATGCAGCTGCTGTGTTTCA AGCTCAGATGACAGTGTGCACAGACTCGCAGCACACACTCAGACATGCGCGACTGCAGTN CTACATACAAGTAGAGTANATCACTAGGGGGATGGGATGATCAGAGCATCAGAGAATTTA CCACCGTTCATCAGGCCAAAACTGTGTGTGAAGAGACTGCAAGGACTCAGGAGGCTGCCC CTGAGGATGT >OriGene 3' read for NM_006790 unedited
ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTAAATATTTG AATTTTATTTTCTTTTGACATACAACTGTGGAACACTCAGATAATCTATACCATGTTAGT ATTCCTACAAGAGAAAACGGGTAAAACAGTATTTAGAAAGCAGTACCAGTAAACGTTATT ACAAGCATTAGATGTGTTTAAAAAGTTGATACTAATTTATAACCTGTAATTGTTAAATGT GCATGACTTAAATGTTAAAATCCTGTATTTTAAGATAGTCTTCTGCAGTTACAAATCCAT ATACCCAGATTTCCTGTAGGCTTCACAAATCGGAGGGGTACATAGAATGTGCAAGAGTCA AAGGATAAATAATATTATAAATATATATTAACTATATTACCTAATTAAAACCATAATCTG TTAATACAGCTATGGATTAATTTCAAAAAAATGTAATCAAATATATTACTAATGGTGTAG TTGGCTGTTTTCCAATGTTGGTAAAGTTATTAAAGTTCTTCACTTTCATAGAGTCCAGAT TGAGCTGCCAAACGCTGAAATTCTCCTTCTGGGTTAAAAGCTTGTTTTACATTCAAACCT TTCCCATTAAGTGCTAAATATTTGCTGAATGTTGGTCGAACCCGTAACTGCTTAGGAGCT GGAAGAGTTTGGTTTGGACGTGCCGTAACGTCTAATCTTGTGGTACATGTAGTCACTCCA GCTTCATTAACTGCTGACACAGTATACCACCCAGCATCTTTCTTGGTTACATCTTTTATC AGTAAAGTACTCTTNCAGTGTTATCTTGATATAAGCTTATTCGGNCAGTGTTGAATTGTA CCATTTCATTATTNCTTTTCAGNAAGCTTTGGTGGANGTATAGNCGAGATCTGGCATTCT AGTTCACTGAATCTCCCTCTAAACTTTTTGCTCGNGGNTNNGAGAAAACATGGGCTCTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006790 |
| Insert Size | 2240 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006790.1, NP_006781.1 |
| RefSeq Size | 2244 bp |
| RefSeq ORF | 1497 bp |
| Locus ID | 9499 |
| UniProt ID | Q9UBF9 |
| Domains | ig, IGc2, IG |
| Gene Summary | This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A naturally monomeric infrared fluorescent protein for protein labeling in vivo
,Yu, D;Baird, MA;Allen, JR;Howe, ES;Klassen, MP;Reade, A;Makhijani, K;Song, Y;Liu, S;Murthy, Z;Zhang, SQ;Weiner, OD;Kornberg, TB;Jan, YN;Davidson, MW;Shu, X;,
Nat. Methods
,PubMed ID 26098020
[MYOT]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202797 | MYOT (Myc-DDK-tagged)-Human myotilin (MYOT), transcript variant 1 |
CNY 3,656.00 |
|
| RC202797L1 | Lenti ORF clone of Human myotilin (MYOT), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202797L2 | Lenti ORF clone of Human myotilin (MYOT), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202797L3 | Lenti ORF clone of Human myotilin (MYOT), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202797L4 | Lenti ORF clone of Human myotilin (MYOT), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202797 | MYOT (tGFP-tagged) - Human myotilin (MYOT), transcript variant 1 |
CNY 5,256.00 |
|
| SC320817 | MYOT (untagged)-Human myotilin (MYOT), transcript variant 1 |
CNY 3,656.00 |
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