ATP5ME (NM_007100) Human Untagged Clone

CAT#: SC115719

ATP5I (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E (ATP5I), nuclear gene encoding mitochondrial protein, transcript variant 1



  "NM_007100" in other vectors (4)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ATP5I mouse monoclonal antibody,clone OTI1E6
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ATP5I; ATP5K
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115719 sequence for NM_007100 edited (data generated by NextGen Sequencing)
ATGGTGCCACCGGTGCAGGTCTCTCCGCTCATCAAGCTCGGCCGCTACTCCGCCCTGTTC
CTCGGTGTGGCCTACGGAGCCACGCGCTACAATTACCTAAAACCTCGGGCAGAAGAGGAG
AGGAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAACTGAAACGGATTGCCAGAGAA
TTGGCAGAAGATGACAGCATATTAAAGTGA

Clone variation with respect to NM_007100.3
>OriGene 5' read for NM_007100 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCAAGGCTTTGCTGG
CTTGTGCGGCATCCTGCTCCGTCTGCAGGTTGTGCTTCCGGTGCGGAGGTCAGGGACAAG
ATGGTGCCACCGGTGCAGGTCTCTCCGCTCATCAAGCTCGGCCGCTACTCCGCCCTGTTC
CTCGGTGTGGCCTACGGAGCCACGCGCTACAATTACCTAAAACCTCGGGCAGAAGAGGAG
AGGAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAACTGAAACGGATTGCCAGAGAA
TTGGCAGAAGATGACAGCATATTAAAGTGAGTGACCCTGCGACCCACTCTTTGGACCAGC
AGCGGATGAATAAAGCTTCCTGTGTTGTGTGAAAAAAAAAAAAAAAAAACTCGACTCTAG
ATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCC
TCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTA
ATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAG
GGGGGTGGTACTGACCCAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGCTCTA
TTGGAACCAACCTGGAGTGCAGTGGCACAATCNTGCCTCACTGCAATCTCCCGCTCCCTG
GGTCAAGCGATTCTCCTTGCTCACCCTCCCGAGTTGTTTGGAATTTCCAGCTTGCCTGAC
CAGGCTCAGCTAATTTTTGGTTTTTTTGTAAGAGACCGGGTNTTAACCATATTTGCCAGG
CTTGTTCCCCAACTCCCTATCTCAGGTGATTTACCCCACTTGGCCCTCCCAATTGGCTGG
ATTACGGCCTGGAACCCTT
>OriGene 3' read for NM_007100 unedited
NTTACTTTGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAACACA
ACACAGGAAGCTTTATTCATCCGCTGCTGGTCCAAAAATGGGTCGCAGGGTCACTCACTT
TAATATGCTGTCATCTTCTGCCAATTCTCTGGCAATCCGTTTCAGTTCATCCTGCTTCTT
CTTCTCTTCTGCTGCTATCCTCCTCTCCTCTTCTGCCCGAGGTTTTAGGTAATTGTAGCG
CGTGGCTCCGTAGGCCACACCGAGGAACAGGGCGGAGTAGCGGCCGAGCTTGATGAGCGG
AGAGACCTGCACCGGTGGCACCATCTTGTCCCTGACCTCCGCACCGGAAGCACAACCTGC
AGACGGAGCAGGATGCCGCACAAGCCAGCAAAGCCTTGGAGCCTCGTGCCGAATTCGCGG
CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG
ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG
GTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCA
AAACCGCATCACCATGGTAATAGCGATGACTAATACCTAGATGTACTGCCAAGTANGAAA
GTCCCGTAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC
AATAGGGGGCGAACTTGGCTATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGT
AAATACTCACCCATTGACGTCAATGGAAAGTCCCTATGGCGTTACTATGGAACCTACCTC
ATTATTGACTCAATGGCCCNGGGGTCTTTGGCGGTTAACCCAGCGGCCCTTTACCGTAGT
TATGTACCCCGCAT
Restriction Sites NotI-NotI     
ACCN NM_007100
Insert Size 380 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007100.2, NP_009031.1
RefSeq Size 368 bp
RefSeq ORF 210 bp
Locus ID 521
UniProt ID P56385
Protein Pathways Metabolic pathways, Oxidative phosphorylation
Gene Summary Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]
Transcript Variant: This variant (1) represents the longer transcript.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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