United States
Germany
Japan
United Kingdom
China
WIF1 (NM_007191) Human Untagged Clone
CAT＃: SC115674
WIF1 (untagged)-Human WNT inhibitory factor 1 (WIF1)
CNY 5,488.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | WIF-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115674 sequence for NM_007191 edited (data generated by NextGen Sequencing)
ATGGCCCGGAGGAGCGCCTTCCCTGCCGCCGCGCTCTGGCTCTGGAGCATCCTCCTGTGC CTGCTGGCACTGCGGGCGGAGGCCGGGCCGCCGCAGGAGGAGAGCCTGTACCTATGGATC GATGCTCACCAGGCAAGAGTACTCATAGGATTTGAAGAAGATATCCTGATTGTTTCAGAG GGGAAAATGGCACCTTTTACACATGATTTCAGAAAAGCACAACAGAGAATGCCAGCTATT CCTGTCAATATCCATTCCATGAATTTTACCTGGCAAGCTGCAGGGCAGGCAGAATACTTC TATGAATTCCTGTCCTTGCGCTCCCTGGATAAAGGCATCATGGCAGATCCAACCGTCAAT GTCCCTCTGCTGGGAACAGTGCCTCACAAGGCATCAGTTGTTCAAGTTGGTTTCCCATGT CTTGGAAAACAGGATGGGGTGGCAGCATTTGAAGTGGATGTGATTGTTATGAATTCTGAA GGCAACACCATTCTCCAAACACCTCAAAATGCTATCTTCTTTAAAACATGTCAACAAGCT GAGTGCCCAGGCGGGTGCCGAAATGGAGGCTTTTGTAATGAAAGACGCATCTGCGAGTGT CCTGATGGGTTCCACGGACCTCACTGTGAGAAAGCCCTTTGTACCCCACGATGTATGAAT GGTGGACTTTGTGTGACTCCTGGTTTCTGCATCTGCCCACCTGGATTCTATGGAGTGAAC TGTGACAAAGCAAACTGCTCAACCACCTGCTTTAATGGAGGGACCTGTTTCTACCCTGGA AAATGTATTTGCCCTCCAGGACTAGAGGGAGAGCAGTGTGAAATCAGCAAATGCCCACAA CCCTGTCGAAATGGAGGTAAATGCATTGGTAAAAGCAAATGTAAGTGTTCCAAAGGTTAC CAGGGAGACCTCTGTTCAAAGCCTGTCTGCGAGCCTGGCTGTGGTGCACATGGAACCTGC CATGAACCCAACAAATGCCAATGTCAAGAAGGTTGGCATGGAAGACACTGCAATAAAAGG TACGAAGCCAGCCTCATACATGCCCTGAGGCCAGCAGGCGCCCAGCTCAGGCAGCACACG CCTTCACTTAAAAAGGCCGAGGAGCGGCGGGATCCACCTGAATCCAATTACATCTGGTGA Clone variation with respect to NM_007191.4 >OriGene 5' read for NM_007191 unedited
AAGTTTAGGATTTTGTATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGG CGGCACGAGGAGTTTTCCCGGCAGCGAGGAGGTCCTGAGCAGCATGGCCCGGAGGAGCGC CTTCCCTGCCGCCGCGCTCTGGCTCTGGAGCATCCTCCTGTGCCTGCTGGCACTGCGGGC GGAGGCCGGGCCGCCGCAGGAGGAGAGCCTGTACCTATGGATCGATGCTCACCAGGCAAG AGTACTCATAGGATTTGAAGAAGATATCCTGATTGTTTCAGAGGGGAAAATGGCACCTTT TACACATGATTTCAGAAAAGCACAACAGAGAATGCCAGCTATTCCTGTCAATATCCATTC CATGAATTTTACCTGGCAAGCTGCAGGGCAGGCAGAATACTTCTATGAATTCCTGTCCTT GCGCTCCCTGGATAAAGGCATCATGGCAGATCCAACCGTCAATGTCCCTCTGCTGGGAAC AGTGCCTCACAAGGCATCAGTTGTTCAAGTTGGTTTCCCATGTCTTGGAAAACAGGATGG GGTGGCAGCATTTGAAGTGGATGTGATTGTTATGAATTCTGAAGGCAACACCATTCTCCA AACACCTCANAATGCTATCTTCTTTAAAACATGTCAACAAGCTGAGTGCCCAGGCGGGTG CCGAAATGGAGGCTTTTGTAATGAAAGACGCATCTGCGAGTGTCCTGATGGGTTCCACGG ACCTCACTGTGAGAAAGCCCTTTGTACCCCACGATGTATGAATGGTGGACTTTGTGTGAC TCCTGGTTTCTGCATCTGCCCACCTGGATTCTATGGAGTGAACTGTGACANAGCANACTG CTCAACCACCTGCTTTAATGGAGGNACCTGTTTCTACCCTGGNAAATGTANNTTGCCTCC CAGACTAGAGGGAGAG >OriGene 3' read for NM_007191 unedited
ACCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAATACTGAATCTCTGGA ATAATGGTAAGGTCAAAATATATTGTATTGAGAGTTTAAAAATTAAGAGCAATTTTTAAA AATGTAACAAACATCTAAATATCTGACAATAAAATCTGAAATGCTGTAACTTCAACATTA ACTGCACCATCCAAATTCTTGTGACTTACGCATTTTTGCCCAATTTAACCTTTCTGATGT TCCCCTGCCCCCAGACACCATAAATGCATTGTAATTTTGAAAATATCTGCCAACTACACA CTGAAAATTTTAACCTGATCAATTGACATAATATAAAATCTGTCCCAAAGCACTGAAACA AGAAAATCTATACCATCATGCTACAGACGTACTTAGAAAACTTAAAAGGAAGAGTAAATA TCAGCTCAGTGATTTATAATGAAGCTAATAAAATTCAGGCCAGTATTCTTAAGTGTAATG AACATTATTTGAACATTCAACACATGAAAGGTTAACAAAGGCTATGAACTTGGTGTAACT TAAAACGTTTCAGATGTCGGAGTTCACCAGATGTAATTGGATTCAGGTGGATCCCGCCGC TCCTCGGCCTTTTTAAGTGAAGGCGTGTGCTGCCTGAGCTGGGCGCCTGCTGGCCTCAGG GCATGTATGACGCTGGCTCCGTACTTTTTATTGCAGTGTCTTCCATGCCAACCTTCTTGA CATTGCATTTGTTGGGTTATGGCAAGTTCCATGTGCACCACGCCAGCCTTGCAAACAGCT TTGAACAAAGTCTCCCTGTAACCTTTGGACATTAAATTGCTTTTACCATGCATTTACCTC CTTTCAACAGGTTGTGGGCATTTGCTGAATTAACCTGTTCTTCCTTTAGCCCGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007191 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007191.2, NP_009122.1 |
| RefSeq Size | 2020 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 11197 |
| UniProt ID | Q9Y5W5 |
| Domains | WIF, EGF, EGF |
| Protein Families | Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway |
| Protein Pathways | Wnt signaling pathway |
| Gene Summary | The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Deregulation of Wnt/ß-catenin signaling through genetic or epigenetic alterations in human neuroendocrine tumors
,Ji Tae Kim, Jing Li, Eun Ryoung Jang, Pat Gulhati, Piotr G. Rychahou, Dana L. Napier, Chi Wang, Heidi L. Weiss, Eun Y. Lee, Lowell Anthony, Courtney M. Townsend, Jr, Chunming Liu, and B.Mark Evers,
Carcinogenesis, May 2013; 34: 953 - 961.
,PubMed ID 23354304
[WIF1]
|
|
The Wnt Receptor Ryk Plays a Role in Mammalian Planar Cell Polarity Signaling
,Maria L. Macheda, Willy W. Sun, Kumudhini Kugathasan, Benjamin M. Hogan, Neil I. Bower, Michael M. Halford, You Fang Zhang, Bonnie E. Jacques, Graham J. Lieschke, Alain Dabdoub, and Steven A. Stacker,
J. Biol. Chem., Aug 2012; 287: 29312 - 29323.
[WIF1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205256 | WIF1 (Myc-DDK-tagged)-Human WNT inhibitory factor 1 (WIF1) |
CNY 5,488.00 |
|
| RC205256L1 | Lenti ORF clone of Human WNT inhibitory factor 1 (WIF1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205256L2 | Lenti ORF clone of Human WNT inhibitory factor 1 (WIF1), mGFP tagged |
CNY 5,890.00 |
|
| RC205256L3 | Lenti ORF clone of Human WNT inhibitory factor 1 (WIF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205256L4 | Lenti ORF clone of Human WNT inhibitory factor 1 (WIF1), mGFP tagged |
CNY 7,888.00 |
|
| RG205256 | WIF1 (tGFP-tagged) - Human WNT inhibitory factor 1 (WIF1) |
CNY 7,088.00 |
