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Plakophilin 3 (PKP3) (NM_007183) Human Untagged Clone
CAT＃: SC115670
PKP3 (untagged)-Human plakophilin 3 (PKP3)
CNY 8,768.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115670 sequence for NM_007183 edited (data generated by NextGen Sequencing)
ATGCAGGACGGTAACTTCCTGCTGTCGGCCCTGCAGCCTGAGGCCGGCGTGTGCTCCCTG GCGCTGCCCTCTGACCTGCAGCTGGACCGCCGGGGCGCCGAGGGGCCGGAGGCCGAGCGG CTGCGGGCAGCCCGCGTCCAGGAGCAGGTCCGCGCCCGCCTCTTGCAGCTGGGACAGCAG CCGCGGCACAACGGGGCCGCTGAGCCCGAGCCTGAGGCCGAGACTGCCAGAGGCACATCC AGGGGGCAGTACCACACCCTGCAGGCTGGCTTCAGCTCTCGCTCTCAGGGCCTGAGTGGG GACAAGACCTCGGGCTTCCGGCCCATCGCCAAGCCGGCCTACAGCCCAGCCTCCTGGTCC TCCCGCTCCGCCGTGGATCTGAGCTGCAGTCGGAGGCTGAGTTCAGCCCACAACGGGGGC AGCGCCTTTGGGGCCGCTGGGTACGGGGGTGCCCAGCCCACCCCTCCCATGCCCACCAGG CCCGTGTCCTTCCATGAGCGCGGTGGGGTTGGGAGCCGGGCCGACTATGACACACTCTCC CTGCGCTCGCTGCGGCTGGGGCCCGGGGGCCTGGACGACCGCTACAGCCTGGTGTCTGAG CAGCTGGAGCCCGCGGCCACCTCCACCTACAGGGCCTTTGCGTACGAGCGCCAGGCCAGC TCCAGCTCCAGCCGGGCAGGGGGGCTGGACTGGCCCGAGGCCACTGAGGTTTCCCCGAGC CGGACCATCCGTGCCCCTGCCGTGCGGACCCTGCAGCGATTCCAGAGCAGCCACCGGAGC CGCGGGGTAGGCGGGGCAGTGCCGGGGGCCGTCCTGGAGCCAGTGGCTCGAGCGCCATCT GTGCGCAGCCTCAGCCTCAGCCTGGCTGACTCGGGCCACCTGCCGGACGTGCATGGGTTC AACAGCTACGGTAGCCACCGAACCCTGCAGAGACTCAGCAGCGGTTTTGATGACATTGAC CTGCCCTCAGCAGTCAAGTACCTCATGGCTTCAGACCCCAACCTGCAGGTGCTGGGAGCG GCCTACATCCAGCACAAGTGCTACAGCGATGCAGCCGCCAAGAAGCAGGCCCGCAGCCTT CAGGCCGTGCCTAGGCTGGTGAAGCTCTTCAACCACGCCAACCAGGAAGTGCAGCGCCAT GCCACAGGTGCCATGCGCAACCTCATCTACGACAACGCTGACAACAAGCTGGCCCTGGTG GAGGAGAACGGGATCTTCGAGCTGCTGCGGACACTGCGGGAGCAGGATGATGAGCTTCGC AAAAATGTCACAGGGATCCTGTGGAACCTTTCATCCAGCGACCACCTGAAGGACCGCCTG GCCAGAGACACGCTGGAGCAGCTCACAGACCTGGTGTTGAGCCCCCTGTCGGGGGCTGGG GGTCCCCCCCTCATCCAGCAGAACGCCTCGGAGGCGGAGATCTTCTACAACGCCACCGGC TTCCTCAGGAACCTCAGCTCAGCCTCTCAGGCCACTCGCCAGAAGATGCGGGAGTGCCAC GGGCTGGTGGACGCCCTGGTCACCTCTATCAACCACGCCCTGGACGCGGGCAAATGCGAG GACAAGAGCGTGGAGAACGCGGTGTGCGTCCTGCGGAACCTGTCCTACCGCCTCTACGAC GAGATGCCGCCGTCCGCGCTGCAGCGGCTGGAGGGTCGCGGCCGCAGGGACCTGGCGGGG GCGCCGCCGGGAGAGGTCGTGGGCTGCTTCACGCCGCAGAGCCGGCGGCTGCGCGAGCTG CCCCTCGCCGCCGATGCGCTCACCTTCGCGGAGGTGTCCAAGGACCCCAAGGGCCTCGAG TGGCTGTGGAGCCCCCAGATCGTGGGGCTGTACAACCGGCTGCTGCAGCGCTGCGAGCTC AACCGGCACACGACGGAGGCGGCCGCCGGGGCGCTGCAGAACATCACGGCAGGCGACCGC AGGTGGGCGGGGGTGCTGAGCCGCCTGGCCCTGGAGCAGGAGCGTATTCTGAACCCCCTG CTAGACCGTGTCAGGACCGCCGACCACCACCAGCTGCGCTCACTGACTGGCCTCATCCGA AACCTGTCTCGGAACGCTAGGAACAAGGACGAGATGTCCACGAAGGTGGTGAGCCACCTG ATCGAGAAGCTGCCGGGCAGCGTGGGTGAGAAGTCGCCCCCAGCCGAGGTGCTGGTCAAC ATCATAGCTGTGCTCAACAACCTGGTGGTGGCCAGCCCCATCGCTGCCCGAGACCTGCTG TATTTTGACGGACTCCGAAAGCTCATCTTCATCAAGAAGAAGCGGGACAGCCCCGACAGT GAGAAGTCCTCCCGGGCAGCATCCAGCCTCCTGGCCAACCTGTGGCAGTACAACAAGCTC CACCGTGACTTCCGGGCGAAGGGCTATCGGAAGGAGGACTTCCTGGGCCCATAG Clone variation with respect to NM_007183.2 >OriGene 5' read for NM_007183 unedited
NTAAAGTTCAGGATTTTGTAATACGACTCCACTATAGGCGGCGCGCGAATTCGCACGAGG CCAGGCCCAGGCCCGGTGGACCTGCCGCGTGCAGGGAGGGGAACTTCCTGCTGTCGGCCC TGCAGCCTGAGGCCGGCGTGTGCTCCCTGGCGCTGCCCTCTGACCTGCAGCTGGACCGCC GGGGCGCCGAGGGGCCGGAGGCCGAGCGGCTGCGGGCAGCCCGCGTCCAGGAGCAGGTCC GCGCCCGCGGGGGGCAGCTGGGACAGCAGCCGCGGCACAACGGGGCCGCTGAGCCCGAGC CTGAGGCCGAGACTGCCAGAGGCACATCCAGGGGGCAGTACCACACCCTGCAGGCTGGCT TCAGCTCTCGCTCTCAGGGCCTGAGTGGGGACAAGACCTCGGGCTTCCGGCCCATCGCCA AGCCGGCCTACAGCCCAGCCTCCTGGTCCTCCCGCTCCGCCGTGGATCTGAGCTGCAGTC GGAGGCTGAGTTCAGCCCACAACGGGGGCAGCGCCTTTGGGGCCGCTGGGTACGGGGGTG CCCAGCCCACCCCTCCCATGCCCACCAGGCCCGTGTCCTTCCATGAGCGCGGTGGGGTTG GGAGCCGGGCCGACTATGACACACTCTCCCTGCGCTCGCTGCGGCTGGGGCCCCGGGGCC TGGACGACCGCTACAGCCTGGTGTCTGAGCAGCTGGAGCCCGCGGCCACCTCCACCTACA GGGGCCTTTGCGTACGAGCGCCAGGCCAGCTCCAGCTCCAGCCGGGCAGGGGGGCTTGGA CTGGCCCCGAGGCCACTGAGGGTTCCCCGAGCCCGGACATCCGTGGCCCCTGGCGTGCGG ACCCTGCGGCGATTCCAGAGCAGCCCCCGGACCCCCGGGGAAGCCGGGCCATGCCCGGGC GCCTTCT >OriGene 3' read for NM_007183 unedited
TTAACGTCCTATTAGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTGAC TGTTCATGGCCATCTTTATTCCCATGCTGGCTATCCCAAGAACTGCCAGGCCACAGCCAA CCCCCACCTCTGCCAATGTGACTGGGTCACCACCCCATACACCAGAGCAGCCTTGAGCCC TGCCCCACCCCCTGCCCTGCGGAAGCCAAGTCCCCAGCTATAAGACCCTGCCCCTCCTGG TGGCCCAGGACCCTCAAAGATGCACACAGGGGCCCCAGCGAGGGGCCCCTCCGTCATTAG CCTTCTCCTCCAGGCTGGGCTGCCAAGCAGCCTGGAGCTGAGTCTGTCCCTTGGACGCTG GGCCACGTCACCTTCTCCTCCAGAAGGCTTCACCTATGGGCCCAGGAAGTCCTCCTTCCG ATAGCCCTTCGCCCGGAAGTCACGGTGGAGCTTGTTGTACTGCCACAGGTTGGCCAGGAA GCTGGATGCTGCCCGGGAGGACTTCTCACTGTCGGGGCTGTCCCGCTTCTTCTTGATGAA GATGAGCTTTCGGAGTCCGTCAAAATACAGCAAGTCTCGGGCAGCGATGGGGCTGGCCAC CACCAGGTTGTTGAGCACAGCTATGATGTTGACCAGCACCTCGGCTGGGGGCGACTTCTC ACCCACGCTGCCCGGCAGCTTCTCGATCAGGTGGCTCACCACCTTCGTGGACATCTCGTC CTTGTTCCTAGCGTTCCGAGACAGGTTTCGGATGATGCCAGTCAGTGAGCGCANCTGGTG GTGGTCGGCGGTCCTGACACGGTCTAGCAAGGGGTTCAGAATACGCTCCTGCTCCAAGGC CA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007183 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007183.2, NP_009114.1 |
| RefSeq Size | 2845 bp |
| RefSeq ORF | 2394 bp |
| Locus ID | 11187 |
| UniProt ID | Q9Y446 |
| Domains | Armadillo_seg |
| Gene Summary | This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may act in cellular desmosome-dependent adhesion and signaling pathways. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) uses an alternate 5' exon structure and differs in the 5' UTR and coding region, compared to variant 2. The resulting isoform (PKP3a) has a shorter and distinct N-terminus compared to isoform PKP3b. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201001 | PKP3 (Myc-DDK-tagged)-Human plakophilin 3 (PKP3) |
CNY 8,752.00 |
|
| RC201001L3 | Lenti-ORF clone of PKP3 (Myc-DDK-tagged)-Human plakophilin 3 (PKP3) |
CNY 7,700.00 |
|
| RC201001L4 | Lenti-ORF clone of PKP3 (mGFP-tagged)-Human plakophilin 3 (PKP3) |
CNY 7,700.00 |
|
| RG201001 | PKP3 (tGFP-tagged) - Human plakophilin 3 (PKP3) |
CNY 6,370.00 |
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