United States
Germany
Japan
United Kingdom
China
PEAMT (PEMT) (NM_007169) Human Untagged Clone
CAT＃: SC115659
PEMT (untagged)-Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 2,400.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PEAMT; PEMPT; PEMT2; PLMT; PNMT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115659 sequence for NM_007169 edited (data generated by NextGen Sequencing)
ATGACCCGGCTGCTGGGCTACGTGGACCCCCTGGATCCCAGCTTTGTGGCTGCCGTCATC ACCATCACCTTCAATCCGCTCTACTGGAATGTGGTTGCACGATGGGAACACAAGACCCGC AAGCTGAGCAGGGCCTTCGGATCCCCCTACCTGGCCTGCTACTCTCTAAGCGTCACCATC CTGCTCCTGAACTTCCTGCGCTCGCACTGCTTCACGCAGGCCATGCTGAGCCAGCCCAGG ATGGAGAGCCTGGACACCCCCGCGGCCTACAGCCTGGGCCTCGCGCTCCTGGGACTGGGC GTCGTGCTCGTGCTCTCCAGCTTCTTTGCACTGGGGTTCGCTGGAACTTTCCTAGGTGAT TACTTCGGGATCCTCAAGGAGGCGAGAGTGACCGTGTTCCCCTTCAACATCCTGGACAAC CCCATGTACTGGGGAAGCACAGCCAACTACCTGGGCTGGGCCATCATGCACGCCAGCCCC ACGGGCCTGCTCCTGACGGTGCTGGTGGCCCTCACCTACATAATGGCTCTCCTATACGAA GAGCCCTTCACCGCTGAGATCTACCGGCAGAAAGCCTCCGGGTCCCACAAGAGGAGCTGA Clone variation with respect to NM_007169.2 523 g=>a >OriGene 5' read for NM_007169 unedited
GAGGTGCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGGCACGAGGTGCAGC GGGGCCCGCGTGGTGCCTCNCTGAGCGGCCCCCGGATGAAGAGATCTGGGAACCCGGGAG CCGAGGTAACGAACAGCTCGGTGGCAGGGCCTGACTGCTGCGGAGGCCTCGGCAATATTG ATTTTAGACAGGCAGACTTCTGCGTTATGACCCGGCTGCTGGGCTACGTGGACCCCCTGG ATCCCAGCTTTGTGGCTGCCGTCATCACCATCACCTTCAATCCGCTCTACTGGAATGTGG TTGCACGATGGGAACACAAGACCCGCAAGCTGAGCAGGGCCTTCGGATCCCCCTACCTGG CCTGCTACTCTCTAAGCGTCACCATCCTGCTCCTGAACTTCCTGCGCTCGCACTGCTTCA CGCAGGCCATGCTGAGCCAGCCCAGGATGGAGAGCCTGGACACCCCCGCGGCCTACAGCC TGGGCCTCGCGCTCCTGGGACTGGGCGTCGTGCTCGTGCTCTCCAGCTTCTTTGCACTGG GGTTCGCTGGAACTTTCCTAGGTGATTACTTCGGGATCCTCAAGGAGGCGAGAGTGACCG TGTTCCCCTTCAACATCCTGGACAACCCCATGTACTGGGGAAGCACAGCCAACTACCTGG GCTGGGCCATCATGCACGCCAGCCCCACGGGCCTGCTCCTGACGGTGCTGGTGGCCCTCA CCTACATAATGGCTCTCCTATACGAAGAGCCCTTCACCGCTGAGATCTACCGGCAGAAAG CCTCCGGGTCCCACAAGAGGAGCTGATTGAGCTGCCACAGCTTTGCCTGAAGGCCTGGCC AGCCTCCTGGCCTGCCCCAGTGGCAAGCCCTGCGCAGGACGAGAAATGTTGCCTGCTGC >OriGene 3' read for NM_007169 unedited
AAAACTTTGGGTGGCCTTTTATGGTGACTGGGAATGTGTGGGTTGGAGCTCAAGGCCTAT GTCGGCACGTCCAGGTCCCCAAGCAGCAGGTTCCAAGGCACTGGGGCAGCCCACGCCGGG GGCGAGCCCTGAGCAGCAGGCACCATTCTCGCCCTGCGCAGGGCCTGCCACTTGGGGCAG GCCAGGAGGCTGGCCAGGCCTTCAGCAAAGCTGTTGCAGCTCAATCAGCTCCTCTTGTGG GACCCGGAGGCTTTCTGCCGGTAGATCTCAGCGGTGAAGGGCTCTTCGTATAGGAGAGCC ATTATGTAGGTGAGGGCCACCAGCACCGTCAGGAGCAGGCCCGTGGGGCTGGCGTGCATG ATGGCCCAGCCCAGGTAGTTGGCTGTGCTTCCCCAGTACATGGGGTTGTCCAGGATGTTG AAGGGGAACACGGTCACTCTCGCCTCCTTGAGGATCCCGAAGTAATCACCTAGGAAAGTT CCAGCGAACCCCAGTGCAAAGAAGCTGGAGAGCACGAGCACGACGCCCAGTCCCAGGAGC GCGAGGCCCAGGCTGTAGGCCGCGGGGGTGTCCAGGCTCTCCATCCTGNGCTGGCTCAGC ATGGCCTGCGTGAAGCAGTGCGAGCGCAGGAAGTTCAGGAGCAGGATGGTGACGCTTAGA GAGTAGCAGGCCAGGTAGGGGGATCCNGAAGCCCTGCTCAGCTTGCGGGTCTTGTNGTCC CATCGTGCAACCACATTCCAGTAAAGCGGATTGAAGGTGATGGTGATGACGGCAGCCACA AAGCTGGGATCCAGGGGGTCCACGTAGCCCAGCAGCCGGGTCATAACGCAGAAGTCTGCC TGTCTAAAATCAATATTGCCGAGGCCTNCGCAGCAGTCAGGCCCCTGCACCGAGCTGTNC GNTACCTCGGCTCCCAGGGTCCCAGATCTCTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007169 |
| Insert Size | 1000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007169.2, NP_009100.2 |
| RefSeq Size | 1008 bp |
| RefSeq ORF | 600 bp |
| Locus ID | 10400 |
| UniProt ID | Q9UBM1 |
| Domains | PEMT |
| Protein Families | Transmembrane |
| Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
| Gene Summary | Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223190 | PEMT (Myc-DDK-tagged)-Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 2,400.00 |
|
| RC223190L1 | Lenti ORF clone of Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC223190L2 | Lenti ORF clone of Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC223190L3 | Lenti ORF clone of Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223190L4 | Lenti ORF clone of Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG223190 | PEMT (tGFP-tagged) - Human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,000.00 |
