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VPS45A (VPS45) (NM_007259) Human Untagged Clone
CAT＃: SC115621
VPS45 (untagged)-Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45)
CNY 4,256.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A; VSP45; VSP45A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115621 sequence for NM_007259 edited (data generated by NextGen Sequencing)
ATGAACGTGGTTTTTGCTGTGAAGCAGTACATTTCCAAAATGATAGAGGACAGCGGGCCT GGTATGAAAGTACTTCTCATGGATAAAGAGACGACTGGCATAGTGAGTATGGTATACACA CAATCGGAGATTCTACAGAAGGAAGTGTACCTCTTTGAACGCATTGATTCTCAAAATCGA GAGATCATGAAACACCTGAAGGCAATTTGTTTTCTTCGACCTACAAAGGAGAATGTGGAT TATATTATTCAGGAGCTCCGAAGACCCAAATACACTATATATTTCATTTATTTCAGTAAT GTGATCAGCAAGAGTGACGTGAAGTCATTGGCTGAAGCTGATGAACAGGAAGTTGTGGCT GAGGTTCAGGAATTTTATGGTGATTACATTGCTGTGAACCCACATTTGTTTTCCCTCAAT ATTTTGGGTTGCTGCCAGGGTCGAAATTGGGATCCAGCCCAGCTATCTAGAACAACTCAA GGGCTTACAGCTCTCCTTTTATCTCTGAAGAAGTGTCCCATGATTCGTTATCAGCTCTCA TCAGAGGCAGCAAAGAGACTTGCAGAGTGCGTTAAGCAAGTGATAACTAAAGAATATGAA CTGTTTGAATTCCGTCGGACAGAGGTTCCTCCATTGCTCCTTATTTTAGATCGCTGTGAT GATGCCATCACCCCATTGCTAAACCAGTGGACATATCAGGCCATGGTCCACGAACTACTA GGCATAAACAACAATCGGATTGATCTTTCCAGAGTGCCGGGAATCAGTAAAGACTTAAGA GAAGTGGTCCTATCTGCTGAAAATGATGAATTCTATGCTAATAATATGTACCTGAACTTT GCTGAGATTGGTAGCAATATAAAGAATCTCATGGAAGATTTTCAGAAGAAGAAACCAAAA GAACAGCAAAAACTAGAATCAATAGCAGACATGAAGGCGTTTGTTGAGAATTATCCACAG TTCAAGAAAATGTCTGGGACTGTTTCAAAGCATGTGACAGTGGTTGGAGAACTGTCTCGA TTGGTCAGTGAACGGAATCTGCTGGAGGTTTCAGAGGTTGAGCAAGAACTGGCCTGTCAA AATGACCATTCTAGTGCTCTCCAGAATATAAAAAGGCTTCTGCAGAACCCCAAAGTGACA GAGTTTGATGCTGCCCGCCTGGTGATGCTTTATGCTTTACATTATGAGCGACACAGCAGC AATAGCCTGCCAGGACTAATGATGGACCTCAGGAATAAAGGTGTTTCTGAGAAGTATCGA AAGCTCGTGTCTGCAGTTGTTGAATATGGTGGTAAACGAGTCAGAGGAAGTGACCTCTTC AGCCCCAAAGATGCTGTGGCTATCACCAAACAATTCCTCAAAGGACTGAAGGGAGTAGAA AATGTATATACACAGCATCAACCTTTCCTACATGAAACCCTGGATCATCTCATCAAAGGA AGGCTTAAGGAAAACCTATATCCTTATTTAGGCCCCAGCACACTCAGAGACAGACCTCAG GATATCATTGTGTTTGTAATTGGAGGAGCCACCTATGAAGAGGCTCTAACAGTTTATAAC CTGAACCGCACCACTCCTGGAGTGAGGATTGTCCTGGGAGGCACCACAGTGCACAACACG AAAAGTTTCCTAGAGGAAGTTCTGGCTTCTGGACTGCACAGCCGAAGCAAGGAGAGCTCT CAAGTCACATCAAGGTCAGCGAGCAGAAGATGA Clone variation with respect to NM_007259.3 >OriGene 5' read for NM_007259 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAATTCGCCGCCA TGAACGTGGTTTTTGCTGTGAAGCAGTACATTTCCAAAATGATAGAGGACAGCGGGCCTG GTATGAAAGTACTTCTCATGGATAAAGAGACGACTGGCATAGTGAGTATGGTATACACAC AATCGGAGATTCTACAGAAGGAAGTGTACCTCTTTGAACGCATTGATTCTCAAAATCGAG AGATCATGAAACACCTGAAGGCAATTTGTTTTCTTCGACCTACAAAGGAGAATGTGGATT ATATTATTCAGGAGCTCCGAAGACCCAAATACACTATATATTTCATTTATTTCAGTAATG TGATCAGCAAGAGTGACGTGAAGTCATTGGCTGAAGCTGATGAACAGGAAGTTGTGGCTG AGGTTCAGGAATTTTATGGTGATTACATTGCTGTGAACCCACATTTGTTTTCCCTCAATA TTTTGGGTTGCTGCCAGGGTCGAAATTGGGATCCAGCCCAGCTATCTAGAACAACTCAAG GGCTTACAGCTCTCCTTTTATCTCTGAAGAAGTGTCCCATGATTCGTTATCAGCTCTCAT CAGAGGCAGCAAAGAGACTTGCAGAGTGCGTTAAGCAAGTGATAACTAAAGAATATGAAC TGTTTGAATTCCGTCCAACAGAGGTTCCTCCATTGCTCCTTATTTTAGATCGCTGTGATG ATGCCATCACCCCATTGCTAAACCAGTGGACATATCATGCCCATGGCCCACGAACTACTA GGCATAAACAACATTCGGATTGATCTTTCCANAGTGCCCGGAATCAGTAAAGACTTAAGA GAGATGGCCCTATCTGCTGCAAATGATGAATT >OriGene 3' read for NM_007259 unedited
CCTGGGAATGGATTCTTATTGTGGTCATACTGGTTTCTCATGCCACAGGAAATTGCTATG TAGGGACAAAAATTTTTGGATGGCTCTGTAAAGAAACATGGTAGGTTTTCAGAAATGAGT TGTGCAGGAATGTGGTTAATGAAAAGCAGAAAGGGTTAAGGGAAGAGAAAGGAAGCCAAG GAGTGTGGTATGTACATCAAATGATTACTTTTTAAGCCCCTCTAGGCTCTGATAACCCTT TCCCCAAGTCATATCCCAACAAAATTCATCAGTAACTGAAGTGATTGTGCTAACAGATAC ATAAAGACTACCGGAGAAAAGTGGGTTGAGATGGGCTCATACTTATTGTTAGGACAACTC TGGGAGTCTTGTGTCTGTGCCAACCACGTATCCGTGGGCTACCTGGAGATGAGTTCTAAC AACCAGCACAGAACCCAAAGCTGCTCTCCAACACCTTTGTTTATTAGGGAAAACCTGTAT TGGGGACAAGAGAGGAATCTGAGCCCTTCCCCCAACCACCGTTTTATTTTCTGCTCGCTG ACCTTGATGTGACTTGAGAGCTCTCCTTGCTTCGGCTGTGCACTCCATAATCCAGAACTT TCTTTAAGGATACTTTTCGTGTTGTGCACTTGGGGTGCCCTCCCATGACAATCCTCACTT CCACGAGTGGTGCCGGGTAAACGCCATAAACTCGCTTAAGGCTCTATCATATGTGGGTTC CTCCCATTCTCATACCCATTGTTATCCTGGAGGTCTTGTTTTTTAGTGCTGCTGGCGCAC CAATTAAGGAATAGGGGCTTTCTCTTACCCTTACCTTGTATGAAGACGATCCACGGCTCG TCTAGTTAGAATAGTGTGATTGCTAAGTTTAATCAATCTAGCCCTCCGCTTAGCGTCCCT TCGAGATATCGTCCGTCTGTTTCCTCTCCTACCTCAGGCTGGGATATATGTACTCCACCT CGCCCCCTTATACCTCCATCTCCAAATCCCCTAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007259 |
| Insert Size | 2340 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007259.2, NP_009190.2 |
| RefSeq Size | 2311 bp |
| RefSeq ORF | 1713 bp |
| Locus ID | 11311 |
| UniProt ID | Q9NRW7 |
| Domains | Sec1 |
| Protein Pathways | Endocytosis |
| Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206027 | VPS45 (Myc-DDK-tagged)-Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45) |
CNY 4,248.00 |
|
| RC206027L1 | Lenti ORF clone of Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45), Myc-DDK-tagged |
CNY 6,648.00 |
|
| RC206027L2 | Lenti ORF clone of Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45), mGFP tagged |
CNY 5,890.00 |
|
| RC206027L3 | Lenti ORF clone of Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206027L4 | Lenti ORF clone of Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45), mGFP tagged |
CNY 5,890.00 |
|
| RG206027 | VPS45 (tGFP-tagged) - Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45) |
CNY 5,848.00 |
