APPL (APPL1) (NM_012096) Human Untagged Clone

Name: APPL (APPL1) (NM_012096) Human Untagged Clone
SKU: SC115543
Price: 5200.00

CAT＃: SC115543

APPL1 (untagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1)

Datasheet

SDS

"NM_012096" in other vectors (6)

CNY 5,200.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

TA807768

APPL1 mouse monoclonal antibody,clone OTI4B11

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APPL; DIP13alpha; MODY14
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC115543 sequence for NM_012096 edited (data generated by NextGen Sequencing) ATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCCGCAGACAAGG TCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATATGAACCAGTTG TATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGCAACACACCTG ACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGGTGATGATGAA GTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAGCTCTTGTCAT GCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCAGTTTAAAGAA AGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAGTAATGATCAT GATGCTGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGACAAGGTGAAG TATGAAGTAACAGAAGATGTGTACACATCCAGAAAGAAACAACACCAGACCATGATGCAT TATTTTTGTGCATTAAATACTCTTCAGTACAAGAAGAAAATAGCATTGTTAGAACCTCTA CTTGGGTACATGCAAGCTCAGATAAGTTTCTTTAAGATGGGTTCTGAAAATCTTAATGAA CAACTGGAAGAATTTTTAGCTAATATTGGAACAAGCGTTCAGAATGTTCGCAGGGAAATG GACAGTGATATAGAGACCATGCAACAGACAATAGAGGATTTGGAAGTAGCCAGTGATCCC TTATATGTGCCTGACCCAGACCCCACCAAATTTCCTGTTAATCGAAATTTAACCCGAAAG GCTGGATACCTTAATGCTAGGAATAAAACAGGCTTGGTGTCATCTACCTGGGACAGACAG TTTTACTTCACGCAGGGTGGAAATTTAATGAGTCAGGCCCGTGGGGATGTAGCAGGAGGC CTGGCCATGGACATAGACAACTGTTCAGTGATGGCTGTGGACTGTGAAGACAGACGATAT TGTTTTCAGATCACCTCTTTCGATGGAAAAAAATCTTCAATTTTGCAAGCAGAGAGTAAA AAAGATCATGAAGAGTGGATCTGTACAATAAACAACATATCTAAACAAATATACTTAAGT GAAAATCCAGAGGAAACTGCTGCACGAGTAAATCAATCAGCTCTGGAAGCTGTCACTCCT TCCCCATCTTTCCAGCAGAGGCACGAGAGCCTGCGGCCAGCAGCAGGACAATCTCGGCCA CCGACAGCTCGAACCAGCAGTTCAGGATCCTTAGGATCTGAGTCTACAAATTTGGCTGCC CTCTCTCTAGATTCTCTTGTTGCCCCAGACACCCCAATACAGTTTGACATAATTTCTCCT GTGTGTGAAGATCAGCCTGGCCAGGCAAAAGCCTTTGGCCAGGGAGGCAGGCGTACAAAT CCATTTGGAGAATCTGGAGGAAGTACAAAATCTGAAACTGAAGATTCTATTCTTCATCAG TTATTTATTGTCCGATTCCTTGGTTCAATGGAGGTGAAATCAGATGACCATCCAGATGTT GTTTATGAAACTATGCGCCAAATCTTAGCTGCCCGGGCCATCCATAACATCTTTCGTATG ACAGAATCGCATTTATTAGTCACTTGTGACTGTTTAAAGTTAATTGATCCACAGACACAA GTTACAAGGCTCACGTTTCCATTACCTTGTGTAGTTTTGTATGCTACACACCAGGAAAAT AAGCGCCTTTTTGGATTTGTTCTTCGGACATCAAGCGGGAGAAGTGAAAGTAATCTGTCA TCAGTCTGCTATATATTTGAGTCAAACAATGAGGGGGAAAAGATATGTGATTCTGTTGGA CTGGCAAAACAGATAGCTTTGCATGCTGAACTGGATCGTAGGGCATCAGAAAAACAAAAA GAAATAGAGAGAGTAAAAGAGAAGCAACAGAAAGAACTCAATAAACAAAAACAGATTGAA AAGGACTTGGAAGAACAAAGTCGGTTGATAGCTGCTTCCAGTAGACCAAACCAAGCCAGT AGTGAGGGGCAGTTTGTTGTCCTTAGCAGTAGCCAGTCAGAAGAGAGTGATTTGGGAGAA GGAGGAAAGAAGAGAGAATCAGAAGCATAA Clone variation with respect to NM_012096.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_012096 unedited TCGGAATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCTGGNAGA AGCTGTGCGGGCGGGGACGGCTGCAGCCCTTGCCGGAGAGGGCGGGCCGGGGTCAGCTGC GGCGGGCGGGCCGGCGCGGGGAGCTGTGGGCGGCAGCTGCGTCTCCTGCCACCGCCCTCC CTCCGCCACGATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCC GCAGACAAGGTCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATAT GAACCAGTTGTATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGC AACACACCTGACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGG TGATGATGAAGTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAG CTCTTGTCATGCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCA GTTTAAAGAAAGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAG TAATGATCATGATGCTGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGA CAAGGGTGAAGTATGAAGTAACAGAAAGATGTGTACACATCCAGAAAGAAACAACACCAG ACCATGATGCATTATTTTTGTGCATTANATACTCTTCAGTACAAGAAGAANATAGCATTG TTAGAACCTCTACTTGNGTACATGNCAGCTCAGATAAGTTTCTTAAGAATGGGGTTCTGA AAATCTTAAATGACACTGGNAGAATTTTTAGCTAATATGGAACAGCGTTCAAATGGTCGC AGGAATGGACAGTGANTAGAGACATGCACAGACATAAGTTTTTGGAGTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_012096 unedited GCTATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTTATT CAGGGGGCAGGTTTTACAACATACTAGCTCTTAGGATCTCTCAGCATTAATAGGATTCAA ATTTTAAATAATATGGTTCCTAGATATAATGCAAAATTAACTCAAATGCCTTTACCATAT ACGATTTTATCTTCCCTAGGATGCACATCCTACTGACCTTTTCCCAAGTATAGTGGAAAC ACATTTACCACCCCTAGATTGTAGAGCCTGAGGATTCTTTTGAAATTAAACAATAATCAT TGCAGATGTGGCAAAAACTAGACTAGCTTTAGGGACTCTGTTCAAGATAATAGCTTTAGT CCCTGGTTGGCAAGTTCTCAAAATTTTTAAAGGTAGAAGGAATAACTGGTTTCCTATCTA CTGACTTCATTGCCATAGTTACAAACATACTGTTCAAATAGGTAACAGAATGCATTGTAG AAGTAGGCAGGCTTAATATCTTTTCTTTTTAAAAAGGAGTTTTGTTGATTTCTAACACCA TTAAGTGTAAGTCTGAATGTGAGAAAAGATATCCCAAAATCGAATCCAGTTACCCTCAAA GCATCTCAGAACTAGAAGGGATTTCAAAGGAGGGTAACAATCAGCTTTACCACTAGGCAG AAACCCATGGGCAGCCTTTTAATAACNAAAAAGAGCTTATTAAAACAGATACTTAAATCT CTGCAGTAATGGAATAGTTCATATAGTTCAACAGTAATGGAAACAAAAGCTGATCATCTA ANATTGNTAAACCCACTCATAACTTCTATTTAGCATTGNAATGCACTTCGATCTGACCTA TGGAGATGAGGGTTTATTTATGTAGAAATNCTGTGAACATGACTTAAACGAACTACAGGT CTGTCAGCATCCCTAGTCTGCCAGNGCCTAACTTTANCTACCTTGACCCAGGTGCTGATA AGGN
Restriction Sites	NotI-NotI
ACCN	NM_012096
Insert Size	5270 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012096.1, NP_036228.1
RefSeq Size	5970 bp
RefSeq ORF	2130 bp
Locus ID	26060
UniProt ID	Q9UKG1
Domains	PH, PID
Protein Pathways	Colorectal cancer, Pathways in cancer
Gene Summary	The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
APPL1 promotes glucose uptake in response to mechanical stretch via the PKC?-non-muscle myosin IIa pathway in C2C12 myotubes ,Saito, T;Okada, S;Shimoda, Y;Tagaya, Y;Osaki, A;Yamada, E;Shibusawa, R;Nakajima, Y;Ozawa, A;Satoh, T;Mori, M;Yamada, M;, Cell. Signal. ,PubMed ID 27478065 [APPL1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC206021	APPL1 (Myc-DDK-tagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,192.00
RC206021L1	Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,592.00
RC206021L2	Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC206021L3	Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC206021L4	Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG206021	APPL1 (tGFP-tagged) - Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,792.00

其它APPL产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies