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Selenophosphate synthetase 2 (SEPHS2) (NM_012248) Human Untagged Clone
CAT＃: SC115458
SEPHS2 (untagged)-Human selenophosphate synthetase 2 (SEPHS2) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
CNY 3,816.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Synonyms | SPS2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115458 sequence for NM_012248 edited (data generated by NextGen Sequencing)
ATGGCGGAAGCCTCGGCGACGGGCGCCTGCGGAGAGGCGATGGCAGCGGCGGAAGGCTCC TCGGGCCCGGCGGGCTTGACTCTGGGCCGGAGCTTCTCGAACTACCGGCCCTTCGAGCCC CAGGCGTTGGGCCTCAGCCCGAGCTGGCGGCTGACGGGCTTCTCCGGCATGAAGGGCTGA GGCTGCAAGGTCCCGCAGGAGGCGCTGCTCAAACTCCTGGCGGGACTGACGCGGCCGGAC GTGCGGCCCCCGCTGGGCCGGGGCCTGGTGGGTGGCCAGGAAGAGGCGTCCCAGGAAGCC GGCCTGCCGGCAGGAGCGGGCCCCAGCCCCACCTTTCCAGCCCTGGGCATCGGGATGGAC TCCTGCGTCATCCCCCTGAGGCACGGGGGCCTGTCACTGGTGCAGACCACGGACTTCTTT TACCCCTTGGTAGAAGATCCCTACATGATGGGGCGCATAGCTTGTGCCAACGTGCTGAGT GACCTCTACGCCATGGGGATTACTGAGTGTGACAACATGTTGATGTTACTCAGCGTCAGC CAGAGTATGAGTGAGGAGGAACGCGAAAAGGTAACGCCACTCATGGTCAAAGGCTTTCGG GATGCGGCTGAGGAAGGAGGGACGGCAGTGACCGGTGGGCAAACGGTGGTCAACCCTTGG ATTATAATCGGTGGAGTTGCCACTGTAGTATGCCAACCAAATGAGTTCATAATGCCGGAC AGCGCCGTCGTTGGGGACGTGCTGGTGTTAACCAAACCGTTAGGAACCCAGGTTGCTGTC AATGCCCACCAATGGCTGGATAATCCTGAAAGATGGAATAAAGTAAAGATGGTGGTCTCC AGAGAAGAGGTGGAGCTGGCCTATCAGGAAGCCATGTTCAATATGGCTACCCTCAACAGA ACTGCTGCAGGTTTAATGCACACATTTAATGCCCATGCGGCCACAGATATCACAGGCTTT GGCATTCTAGGACACTCCCAGAACCTTGCAAAACAACAAAGAAATGAAGTGTCCTTTGTT ATTCATAATCTGCCAATAATTGCCAAGATGGCTGCCGTCAGCAAGGCCAGTGGACGGTTT GGGCTTCTTCAAGGAACCTCAGCTGAAACCTCTGGGGGATTACTGATTTGTCTGCCAAGA GAACAGGCGGCTCGCTTTTGTTCTGAAATCAAATCCTCCAAGTACGGAGAGGGTCACCAA GCGTGGATCGTTGGCATTGTGGAAAAGGGAAACCGAACGGCCCGGATCATTGACAAGCCG CGAGTTATTGAAGTCCTGCCTCGTGGGGCCACAGCTGCTGTTCTTGCTCCTGACAGTTCA AATGCCTCCTCTGAGCCTAGCTCGTGA Clone variation with respect to NM_012248.2 >OriGene 5' read for NM_012248 unedited
AATTTGGTAAACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGACGGCTTGAG TAGCGCTAGGGAGAATCCCTGCAGGTAATATTTGACTTTTGCTTCATATTAATCTGAGTG GAAAATAAAAGGGCCCTCTTCTCCTCTCGCTTCCCTGCCGGGCAGGCGCCATGGCGGAAG CCTCGGCGACGGGCGCCTGCGGAGAGGCGATGGCAGCGGCGGAAGGCTCCTCGGGCCCGG CGGGCTTGACTCTGGGCCGGAGCTTCTCGAACTACCGGCCCTTCGAGCCCCAGGCGTTGG GCCTCAGCCCGAGCTGGCGGCTGACGGGCTTCTCCGGCATGAAGGGCTGAGGCTGCAAGG TCCCGCAGGAGGCGCTGCTCAAACTCCTGGCGGGACTGACGCGGCCGGACGTGCGGCCCC CGCTGGGCCGGGGCCTGGTGGGTGGCCAGGAAGAGGCGTCCCAGGAAGCCGGCCTGCCGG CAGGAGCGGGCCCCAGCCCCACCTTTCCAGCCCTGGGCATCGGGATGGACTCCTGCGTCA TCCCCCTGAGGCACGGGGGCCTGTCACTGGTGCAGACCACGGACTTCTTTTACCCCTTGG TAGAAGATCCCTACATGATGGGGCGCATAGCTTGTGCCAACGTGCTGAGTGACCTCTACG CCATGGGGATTACTGAGTGTGACAACATGTTGATGTTACTCAGCGTCAGCCAGAGTATGA GTGAGGAGGAACGCGAAAAGTAACGCCACTCATGGTCAAAGGCTNTCGGAATGCGGCTGA NGAAGAGGNACGGCAGTGACCGGTGGGCCAACGGTGGTCAACCCTTGGATTATAATCGGT GGAGTTGCCACTGTAGTATGCCAACCAAATGAGTNCATAATGCCGNACAGCGCCGTCGTT GGGGACCTGCCTGGGTTAAACCAACCGTAAGGACCCACGTTGCTGTTCATGCCCACCAAT GGCTGGAAATCCTGGAGNAGGATAAG >OriGene 3' read for NM_012248 unedited
TAGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTAGTTTTTCAACAAGGTTTCTTTTATTTATTTAGCAAGAAATTACTCTTTATTGAT CAAATCACCAGGAATCTGCCGCAAAAGACTTAACATCTTAATGACCACTGAGGCCTGGGC TCTGAGGCTTGCTCTGCCTCTCCCAGTCCTTAATGGGGTTTAGAGGGAGAGACGTCATTA ACAGAGACCAAGTCAGATGGTTGCAGGTCCTCCTTTCCCAGTCATCAGACAAGTTGCTCA ATTCTTGTCCAGAGCAGGAAAGAGCCAGTACTTCAGTAAAATTGAATAAAGAATTTGATG TTGCATTATAAGATCTCTCTTAACAATTTAAATTTATCAACAATATTGGTCCAAAACTCA TTTGCTTCTAATTTTTTTCATTTTGCAATTTACTTTGGGTGTGTGTTACTTTAATAATCT CACTCCCTGTGCAGAAATTAATCAACCCCGTGCAAAGAAAAATCCCTCAACCTGGAATAG CTTCATCTTACTTTGGCAAGAGAAAACAGAGGTATTTTTGCTCCTCTTCTACCCAAGTGA TGAACAAATATCCCCCCAAAGTTCAAAATGTTACTCTGGCCTCGGAATGCATATAAAGAC TGCTTTAGATGGGCTGATTCCAATCACCTAGAAAGGGCAGCCGGAACCACTATGCAGGCA GCCTTCTTTGGAAATTTCTTACATCAACTCTTGAGACCATCCGTGATTGTGGACAATGGC TCTAGGTTCAAACAACTTCTGTTCTTTCATCTCACGAGCTAGGCTCAGAAGAAGCATTTT GACTGTCAGGAGCAAGAACAGCAGCTGTGGGCCCACGAGCCAGGACTTCCATACTCGCGG CTTGCAATGATCCGGCCCGTCGGTTCCCTTTTCACATGCCACGATCACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012248 |
| Insert Size | 2240 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012248.2, NP_036380.2 |
| RefSeq Size | 2291 bp |
| Locus ID | 22928 |
| UniProt ID | Q99611 |
| Domains | AIRS |
| Protein Pathways | Metabolic pathways, Selenoamino acid metabolism |
| Gene Summary | This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. A pseudogene for this locus has been identified on chromosome 5. [provided by RefSeq, May 2017] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201830 | SEPHS2 (Myc-DDK-tagged)-Human selenophosphate synthetase 2 (SEPHS2), (Note, selenocysteine protein, internal stop codon, see reference data summary) |
CNY 3,656.00 |
|
| RC201830L1 | Lenti-ORF, SEPHS2 (Myc-DDK-tagged)-Human selenophosphate synthetase 2 (SEPHS2), (Note, selenocystein protein, internal stop codon, see summary) |
CNY 6,056.00 |
|
| RG201830 | SEPHS2 (GFP-tagged) - Human selenophosphate synthetase 2 (SEPHS2), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 5,256.00 |
|
| SC320578 | SEPHS2 (untagged)-Human selenophosphate synthetase 2 (SEPHS2) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,816.00 |
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