DNA Polymerase lambda (POLL) (NM_013274) Human Untagged Clone
CAT#: SC115324
POLL (untagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2
CNY 4,288.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BETAN; POLKAPPA |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115324 sequence for NM_013274 edited (data generated by NextGen Sequencing)
ATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTGATGCA TCATCAAAAGTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAGAGTGG CTGAGCTCCCTTCGGGCCCATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAGAACTC TTTGAGAAGCAGATTGTTCAGCATGGCGGCCAGCTATGCCCTGCCCAGGGCCCAGGTGTC ACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAGCCCTCCGCCTTCTCAGACTA CCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTGCCTTCAG GAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACTTGGAC CATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATGAGGCC CTGCTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTCCCCAA AAGGCAAAAGAGGCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCAGTGAT GGGGAAGAAACCCAGGTTAGTGCAGCTGATCTGGAAGCCCTCATCAGTGGCCACTACCCC CCCTCCCTTGAGGGAGATTGTGAGCCTAGCCCAGCCCCTGCTGTCCTGGATAAGTGGGTC TGTGCACAGCCCTCAAGCCAGAAGGCGACCAATCACAACCTCCATATCACAGAGAAGCTG GAAGTTCTGGCCAAAGCCTACAGTGTTCAGGGAGACAAGTGGAGGGCCCTGGGCTATGCC AAGGCCATCAATGCCCTCAAGAGCTTCCATAAGCCTGTCACCTCGTACCAGGAGGCCTGC AGTATCCCTGGGATTGGGAAGCGGATGGCTGAGAAAATCATAGAGATCCTGGAGAGCGGG CATTTGCGGAAGCTGGACCATATCAGTGAGAGCGTGCCTGTCTTGGAGCTCTTCTCCAAC ATCTGGGGAGCTGGGACCAAGACTGCCCAGATGTGGTACCAACAGGGCTTCCGAAGTCTG GAAGACATCCGCAGCCAGGCCTCCCTGACAACCCAGCAGGCCATCGGCCTGAAGCATTAC AGTGACTTCCTGGAACGTATGCCCAGGGAGGAGGCTACAGAGATTGAGCAGACAGTCCAG AAAGCAGCCCAGGCCTTTAACTCCGGGCTGCTGTGTGTGGCATGTGGTTCATACCGACGG GGAAAGGCGACCTGTGGTGATGTCGACGTGCTCATCACTCACCCAGATGGCYGGTCCCAC CGGGGTATCTTCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATGAC TTGGTGAGCCAAGAGGAGAATGGTCAGCAACAGAAGTACTTGGGGGTGTGCCGGCTCCCA GGGCCAGGGCGGCGGCACCGGCGCCTGGACATCATCGTGGTGCCCTATAGCGAGTTTGCC TGTGCCCTGCTCTACTTCACCGGCTCTGCACACTTCAACCGCTCCATGCGAGCCCTGGCC AAAACCAAGGGCATGAGTCTGTCAGAACATGCCCTCAGCACTGCTGTGGTCCGGAACACC CATGGCTGCAAGGTGGGGCCTGGCCGAGTGCTGCCCACTCCCACTGAGAAGGATGTCTTC AGGCTCTTAGGCCTCCCCTACCGAGAACCTGCTGAGCGGGACTGGTGA Clone variation with respect to NM_013274.3 661 a=>c;1224 t=>c;1312 c=>y >OriGene 5' read for NM_013274 unedited
NCTTGCAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCAA TTCTGGATTTCCACGTGCAGCAGCCCTGACCACGCTCCAATAGGCCGGGATCCAGCCATA CTTCAATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTG ATGCATCATCAAAAGTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAG AGTGGCTGAGCTCCCTTCGGGCCCATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAG AACTCTTTGAGAAGCAGATTGTTCAGCATGGCGGCCAGCTATGCCCTGCCCAGGGCCCAG GTGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAGCCCTCCGCCTTCTCA GACTACCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTGCC TTCAGGAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACT TGGACCATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATG AGGCCCTGCTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTC CCCAAAAGGCAAAAGAGGCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCA GTGATGGGGAAGAAACCCAGGTTAGTGCAGCTGATCTGGAAGCCCTCATCAGTGGCCACT ACCCCCCCTCCCTTGAGGGNAGATTGTGAGCCTAGCCCAGCCCCTGCTGTCCTGGATAAG TGGGGTCTGTGCACAGCCCTCAAGCCAGAAGCGACCAATCACAACCTNCATATCACAGAG AAGCTGGNAAGTTCTGGGCCAAGCCTACAGTGNTCAGGNAGACCAAGTGGNAGGCCCNNT GGCTTC >OriGene 3' read for NM_013274 unedited
NTTCTACTTGNACCCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGGTTC AATGTCAAATGCACTTTACTGGGCCCCTGGGCCTCCCAACACTGTCCTCACCAAGGCTGG GGCACCCCATTCCCAGCACCACCAGCTGACCGGGAAGCAGGACGGATAGAGGGAACACAT GTGGAATGGCTGTTCTCCTACCCCAAAGGTGCCGGATGATGTTGACAGCACGGCTGCTTC CCTCTCCCCTGCAGCTTCTGTCCCCACCCAGCAGCTTGGCCTTTCCCATAGCAGGGAAGG GGGATACCTGGGGCTTCAAACCAGCCACCTGCTCCTGCTTAAAATGGGGCAAGGGGCCAT TTGCAAAATTCTTCGAGGGGCAGTGGTGAGGTAAAAGGGGTGGCAGCCTGCTCCTGTCTG GGAGAGGGCTGGGCAGACACTGGGAGCCGGGCAGACACTGGGAGCCGGGCTGGCTCTTGC TTCAGGCCCAGAGCCCTGGGCCCTGCTCGCTGAGGAAGCTGGTGGTTGGAGCGGCGAGGT TCAACCAGCTGAGGCTGGAAGGAGTACTGGGTGGCCAGGAGGGGTAGCCAGTCCAACTCG GCTCTCCTCAGCACCCCCAGCCATGGGTCACCAGTCCCGCTCAGCAGGTTCTCGGTAGGG GAGGCCTAAGAGCCTGAAGACATCCTTCTCAGTGGGAGTGGGCAGCACTCGGCCAGGCCC CACCTTGCAGCCATGGGGTGTTCCGGACCACAGCAGTGCTGAGGGCATGTTCTGACAGAC TCATGCCCTTTGGTTNTGGCCCAGGCTCGCATGGAGCGGNTGAAGTGTGCAAAGCCGGTG AAGTAAAGCAGGGCACAGGCAAACTCGCTATAGGGCACCACGATGATGTCCAGGCGCCGG TGCCGNCGCCTGGCCCTGGGAGCCNGCACACCCCC |
Restriction Sites | NotI-NotI |
ACCN | NM_013274 |
Insert Size | 3460 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013274.2, NP_037406.1 |
RefSeq Size | 2702 bp |
RefSeq ORF | 1728 bp |
Locus ID | 27343 |
UniProt ID | Q9UGP5 |
Domains | POLXc |
Protein Families | Druggable Genome |
Protein Pathways | Base excision repair, Non-homologous end-joining |
Gene Summary | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a). |
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Other Versions
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RC209816 | POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2 |
CNY 4,280.00 |
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RC209816L3 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
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RC209816L4 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
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RG209816 | POLL (tGFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 2 |
CNY 5,040.00 |
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