DNA Polymerase lambda (POLL) (NM_013274) Human Untagged Clone

CAT#: SC115324

POLL (untagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2



  "NM_013274" in other vectors (4)

CNY 4,288.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal DNA Polymerase lambda antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BETAN; POLKAPPA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115324 sequence for NM_013274 edited (data generated by NextGen Sequencing)
ATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTGATGCA
TCATCAAAAGTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAGAGTGG
CTGAGCTCCCTTCGGGCCCATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAGAACTC
TTTGAGAAGCAGATTGTTCAGCATGGCGGCCAGCTATGCCCTGCCCAGGGCCCAGGTGTC
ACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAGCCCTCCGCCTTCTCAGACTA
CCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTGCCTTCAG
GAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACTTGGAC
CATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATGAGGCC
CTGCTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTCCCCAA
AAGGCAAAAGAGGCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCAGTGAT
GGGGAAGAAACCCAGGTTAGTGCAGCTGATCTGGAAGCCCTCATCAGTGGCCACTACCCC
CCCTCCCTTGAGGGAGATTGTGAGCCTAGCCCAGCCCCTGCTGTCCTGGATAAGTGGGTC
TGTGCACAGCCCTCAAGCCAGAAGGCGACCAATCACAACCTCCATATCACAGAGAAGCTG
GAAGTTCTGGCCAAAGCCTACAGTGTTCAGGGAGACAAGTGGAGGGCCCTGGGCTATGCC
AAGGCCATCAATGCCCTCAAGAGCTTCCATAAGCCTGTCACCTCGTACCAGGAGGCCTGC
AGTATCCCTGGGATTGGGAAGCGGATGGCTGAGAAAATCATAGAGATCCTGGAGAGCGGG
CATTTGCGGAAGCTGGACCATATCAGTGAGAGCGTGCCTGTCTTGGAGCTCTTCTCCAAC
ATCTGGGGAGCTGGGACCAAGACTGCCCAGATGTGGTACCAACAGGGCTTCCGAAGTCTG
GAAGACATCCGCAGCCAGGCCTCCCTGACAACCCAGCAGGCCATCGGCCTGAAGCATTAC
AGTGACTTCCTGGAACGTATGCCCAGGGAGGAGGCTACAGAGATTGAGCAGACAGTCCAG
AAAGCAGCCCAGGCCTTTAACTCCGGGCTGCTGTGTGTGGCATGTGGTTCATACCGACGG
GGAAAGGCGACCTGTGGTGATGTCGACGTGCTCATCACTCACCCAGATGGCYGGTCCCAC
CGGGGTATCTTCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATGAC
TTGGTGAGCCAAGAGGAGAATGGTCAGCAACAGAAGTACTTGGGGGTGTGCCGGCTCCCA
GGGCCAGGGCGGCGGCACCGGCGCCTGGACATCATCGTGGTGCCCTATAGCGAGTTTGCC
TGTGCCCTGCTCTACTTCACCGGCTCTGCACACTTCAACCGCTCCATGCGAGCCCTGGCC
AAAACCAAGGGCATGAGTCTGTCAGAACATGCCCTCAGCACTGCTGTGGTCCGGAACACC
CATGGCTGCAAGGTGGGGCCTGGCCGAGTGCTGCCCACTCCCACTGAGAAGGATGTCTTC
AGGCTCTTAGGCCTCCCCTACCGAGAACCTGCTGAGCGGGACTGGTGA

Clone variation with respect to NM_013274.3
661 a=>c;1224 t=>c;1312 c=>y
>OriGene 5' read for NM_013274 unedited
NCTTGCAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCAA
TTCTGGATTTCCACGTGCAGCAGCCCTGACCACGCTCCAATAGGCCGGGATCCAGCCATA
CTTCAATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTG
ATGCATCATCAAAAGTACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAG
AGTGGCTGAGCTCCCTTCGGGCCCATGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAG
AACTCTTTGAGAAGCAGATTGTTCAGCATGGCGGCCAGCTATGCCCTGCCCAGGGCCCAG
GTGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAGCCCTCCGCCTTCTCA
GACTACCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTTGTGCC
TTCAGGAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACT
TGGACCATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATG
AGGCCCTGCTTCAGACAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTC
CCCAAAAGGCAAAAGAGGCACCAAACACCCAAGCCCAGCCCATCTCTGATGATGAAGCCA
GTGATGGGGAAGAAACCCAGGTTAGTGCAGCTGATCTGGAAGCCCTCATCAGTGGCCACT
ACCCCCCCTCCCTTGAGGGNAGATTGTGAGCCTAGCCCAGCCCCTGCTGTCCTGGATAAG
TGGGGTCTGTGCACAGCCCTCAAGCCAGAAGCGACCAATCACAACCTNCATATCACAGAG
AAGCTGGNAAGTTCTGGGCCAAGCCTACAGTGNTCAGGNAGACCAAGTGGNAGGCCCNNT
GGCTTC
>OriGene 3' read for NM_013274 unedited
NTTCTACTTGNACCCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGGTTC
AATGTCAAATGCACTTTACTGGGCCCCTGGGCCTCCCAACACTGTCCTCACCAAGGCTGG
GGCACCCCATTCCCAGCACCACCAGCTGACCGGGAAGCAGGACGGATAGAGGGAACACAT
GTGGAATGGCTGTTCTCCTACCCCAAAGGTGCCGGATGATGTTGACAGCACGGCTGCTTC
CCTCTCCCCTGCAGCTTCTGTCCCCACCCAGCAGCTTGGCCTTTCCCATAGCAGGGAAGG
GGGATACCTGGGGCTTCAAACCAGCCACCTGCTCCTGCTTAAAATGGGGCAAGGGGCCAT
TTGCAAAATTCTTCGAGGGGCAGTGGTGAGGTAAAAGGGGTGGCAGCCTGCTCCTGTCTG
GGAGAGGGCTGGGCAGACACTGGGAGCCGGGCAGACACTGGGAGCCGGGCTGGCTCTTGC
TTCAGGCCCAGAGCCCTGGGCCCTGCTCGCTGAGGAAGCTGGTGGTTGGAGCGGCGAGGT
TCAACCAGCTGAGGCTGGAAGGAGTACTGGGTGGCCAGGAGGGGTAGCCAGTCCAACTCG
GCTCTCCTCAGCACCCCCAGCCATGGGTCACCAGTCCCGCTCAGCAGGTTCTCGGTAGGG
GAGGCCTAAGAGCCTGAAGACATCCTTCTCAGTGGGAGTGGGCAGCACTCGGCCAGGCCC
CACCTTGCAGCCATGGGGTGTTCCGGACCACAGCAGTGCTGAGGGCATGTTCTGACAGAC
TCATGCCCTTTGGTTNTGGCCCAGGCTCGCATGGAGCGGNTGAAGTGTGCAAAGCCGGTG
AAGTAAAGCAGGGCACAGGCAAACTCGCTATAGGGCACCACGATGATGTCCAGGCGCCGG
TGCCGNCGCCTGGCCCTGGGAGCCNGCACACCCCC
Restriction Sites NotI-NotI     
ACCN NM_013274
Insert Size 3460 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013274.2, NP_037406.1
RefSeq Size 2702 bp
RefSeq ORF 1728 bp
Locus ID 27343
UniProt ID Q9UGP5
Domains POLXc
Protein Families Druggable Genome
Protein Pathways Base excision repair, Non-homologous end-joining
Gene Summary This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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