SART2 (DSE) (NM_013352) Human Untagged Clone

CAT#: SC115299

DSE (untagged)-Human dermatan sulfate epimerase (DSE), transcript variant 1



  "NM_013352" in other vectors (4)

CNY 7,024.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
DSE Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DS-epi1; DSEP; DSEPI; EDSMC2; SART-2; SART2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115299 sequence for NM_013352 edited (data generated by NextGen Sequencing)
ATGAGGACTCACACACGGGGGGCTCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTG
TCAGCCTACATCACCGACGAGAACCCAGAAGTTATGATTCCCTTCACCAATGCCAACTAC
GACAGCCATCCCATGCTGTACTTCTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCT
GCCAGCTCGCACGAGCACATTGCAGCCCGCCTCACGGAGGCTGTGCACACGATGCTGTCC
AGCCCCTTGGAATACCTCCCTCCCTGGGATCCCAAGGACTACAGTGCCCGCTGGAATGAA
ATTTTTGGAAACAACTTGGGTGCCTTGGCAATGTTCTGTGTGCTGTATCCTGAGAACATT
GAAGCCCGAGACATGGCCAAAGACTACATGGAGAGGATGGCAGCGCAGCCTAGTTGGTTG
GTGAAAGATGCTCCTTGGGATGAGGTCCCGCTTGCTCACTCCCTGGTTGGTTTTGCCACT
GCTTATGACTTCTTGTACAACTACCTGAGCAAGACACAACAGGAGAAGTTTCTTGAAGTG
ATTGCCAATGCCTCAGGGTATATGTATGAAACTTCATACAGGAGAGGATGGGGATTTCAA
TACCTGCACAATCATCAGCCCACCAACTGTATGGCTTTGCTCACGGGAAGCCTAGTCCTG
ATGAATCAAGGATATCTTCAAGAAGCCTACTTATGGACCAAACAAGTTCTGACCATCATG
GAGAAATCTCTGGTCTTGCTCAGGGAGGTGACGGATGGCTCCCTCTATGAAGGAGTTGCG
TATGGCAGCTACACCACTAGATCACTCTTCCAATACATGTTTCTCGTCCAGAGGCACTTC
AACATCAACCACTTTGGCCATCCGTGGCTTAAACAACACTTTGCATTTATGTATAGAACC
ATCCTGCCAGGGTTTCAAAGGACTGTGGCTATTGCGGACTCAAATTACAACTGGTTTTAT
GGTCCAGAAAGCCAATTAGTGTTCCTTGATAAATTTGTCATGCGTAATGGCAGTGGTAAC
TGGCTAGCTGACCAAATCAGAAGGAACCGTGTGGTGGAAGGTCCAGGAACACCATCCAAA
GGGCAGCGCTGGTGCACTCTGCACACAGAATTTCTCTGGTATGATGGCAGCTTGAAATCG
GTTCCTCCTCCAGACTTTGGCACCCCTACACTGCATTATTTTGAAGACTGGGGTGTCGTG
ACTTATGGAAGTGCACTACCTGCAGAAATCAATAGATCTTTCCTTTCCTTCAAGTCTGGA
AAACTGGGGGGACGTGCAATATATGACATTGTCCACAGAAACAAATACAAAGATTGGATC
AAAGGATGGAGAAATTTTAATGCAGGGCATGAACATCCTGATCAAAACTCATTTACTTTT
NCTCCCAATGGTGTGCCTTTCATTACTGAGGCTCTGTACGGGCCAAAGTACACCTTCTTC
AACAATGTTTTGATGTTTTCCCCAGCTGTGTCAAAGAGCTGCTTTTCTCCCTGGGTGGGT
CAGGTCACAGAAGACTGCTCATCAAAATGGTCTAAATACAAGCATGACCTGGCAGCTAGT
TGTCAGGGGAGGGTGGTTGCAGCAGAGGAGAAAAATGGGGTGGTTTTCATCCGAGGAGAA
GGTGTGGGAGCTTATAACCCCCAGCTCAACCTGAAGAATGTTCAGAGGAATCTCATCCTC
CTACATCCACAGCTGCTTCTCCTTGTAGATCAAATACACCTGGGAGAGGAGAGTCCCTTG
GAGACAGCAGCGAGCTTCTTCCATAATGTGGATGTTCCTTTTGAGGAGACTGTGGTAGAT
GGTGTCCATGGGGCTTTCATCAGGCAGAGAGATGGTCTCTATAAAATGTACTGGATGGAC
GATACTGGCTACAGCGAGAAAGCAACCTTTGCCTCAGTGACATATCCTCGGGGCTATCCC
TACAACGGGACAAACTATGTGAATGTCACCATGCACCTCCGAAGTCCCATCACCAGGGCA
GCTTACCTCTTCATAGGGCCATCTATAGATGTTCAGAGCTTCACTGTCCACGGAGACTCT
CAGCAACTGGATGTGTTCATAGCCACCAGCAAACATGCCTACGCCACATACCTGTGGACA
GGTGAGGCCACAGGACAGTCTGCCTTTGCACAGGTCATTGCTGATCGTCACAAAATTCTG
TTTGACCGGAATTCAGCCATCAAGAGCAGCATTGTCCCTGAGGTGAAGGACTATGCTGCT
ATTGTGGAACAGAACTTGCAGCATTTTAAACCAGTGTTTCAGCTGCTGGAGAAGCAGATA
CTGTCCCGAGTCCGGAACACAGCTAGCTTTAGGAAGACTGCTGAACGCCTGCTGAGATTT
TCAGATAAGAGACAGACTGAGGAGGCCATTGACAGGATTTTTGCCATATCACAGCAACAG
CAGCAGCAAAGCAAGTCAAAGAAAAACCGAAGGGCAGGCAAACGCTATAAATTTGTGGAT
GCTGTCCCTGATATTTTTGCACAGATTGAAGTCAATGAGAAAAAGATTAGACAGAAAGCT
CAGATTTTGGCACAGAAAGAACTACCCATAGATGAAGATGAAGAAATGAAAGACCTTTTA
GATTTTGCAGATGTAACATACGAGAAACATAAAAATGGGGGCTTGATTAAAGGCCGGTTT
GGACAGGCACGGATGGTGACAACTACACACAGCAGGGCCCCATCACTGTCTGCTTCCTAT
ACCAGGTTGTTCCTGATTCTGAACATTGCTATTTTCTTTGTCATGTTGGCAATGCAACTG
ACTTATTTCCAGAGGGCCCAGAGCCTACATGGCCAAAGATGTCTTTATGCAGTTCTTCTC
ATAGATAGCTGTATTTTATTATGGTTGTACTCTTCTTGTTCCCAATCACAGTGTTAG

Clone variation with respect to NM_013352.2
1381 g=>n;1710 c=>t
>OriGene 5' read for NM_013352 unedited
GCGAATTCGGCACGAGGCATCCCGGGGCATGGCGCGGCGGGGGCGCGGAGGGCTCGGTTC
GGAGGGGGCCGGGAGCCCGGGCGCCCTGGAGTGAGGAGGACCGGGAGCTGGCTCTGGAGG
CTGCGGAGGCGACGCCGGAGAGAACGAAGCCTCGGCTGGGAGCGGATCTTTCGAAGATGG
TTTGGCTGCCTTGGAGATTTGGAGATCTGGTGCCACGATGAGGACTCACACACGGGGGGC
TCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTGTCAGCCTACATCACCGACGAGAA
CCCAGAAGTTATGATTCCCTTCACCAATGCCAACTACGACAGCCATCCCATGCTGTACTT
CTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCTGCCAGCTCGCACGAGCACATTGC
AGCCCGCCTCACGGAGGCTGTGCACACGATGCTGTCCAGCCCCTTGGAATACCTCCCTCC
CTGGGATCCCAAGGACTACAGTGCCCGCTGGAATGAAATTTTTGGAAACAACTTGGGTGC
CTTGGCAATGTTCTGTGTGCTGTATCCTGAGAACATTGAAGCCCGAGACATGGCCAAAGA
CTACATGGAGAGGATGGCAGCGCAACCTAGTTGGTTGGGAAAGATGCTCCTTGGGATGAG
GTCCCGCTTGCTCACTCCCTGGGTGGTTTTGCCACTGCTTATGACTTCTTNGTACACTAC
CTGAGNCAGACACACAGGAGAAGTTTCTTG
>OriGene 3' read for NM_013352 unedited
TTTGTACCGAGCGTCCNGTCCAGGATCGAGATATATCTTTTTTTTTTTNGAAAATTCACA
ATTATGATGTTTATTTAGGGAAAATAAACTTGAAGAAGGTTTTCTATTAAAAAAACATAA
GAATCATATCCAAATTTTAGACTCTCCATCTTCTTTGGTATGAAAATCAACATTCATAAT
AGAAAATATTTTCTTTTGAAAGGGTTAGTTGGGAGTGGGTCTATAACGGTTTTCCATATA
AGGAAAATACAGTATTATCTGTAATCATCTCACTTATTGAACAACCAATGAATGCATGCA
AGTAACCAAGCATATGTGTGCAGAGACCAGCATCAAGGAAATATTCATTCATAGATAAAT
GTTTTCTAATAAAATATATTTTAAGTTTTTAATGATCTTCTGCTATCATTTTTTGCCATG
TTATCTGAATAGCCATCCAGATCTGTAGTAAAACATCCTGTTAGTTACATAACTCAAGAA
GTACTTCCCCAAGGAAGCTCACACCATCATACCAGGACTTAGCAACATTTATCTTTCAAC
ATGAGTATACCATTAAAATTTTTTTATATTTCTCAGAATTGCATTCTTCAGTCTTTTCCC
CATATAGTCTATGGAAATTGCTAATACCATTAAAATAATATTAATAAAACCCAATTCTAT
CTCAACCTTTTTCTCATAAGACAAAACCTTACTTTTAACAATAGACTTTCTGGTTCTTCT
CCACAGTCTCCTCTACGGACCGCTCACTGCGCATGCCCAATCCTTTCAAAACCCCCTCGG
CCCCCACGCTATTGCCACCGCTTGTTCCCCCTTTTTCTCCCCCTTCTTTCCCCGCCCGTT
TCTTGCGCCGTATCCCTCCTTTTTTTCGGCCCCGACGCGGCGGGCCACTCTGTTCCTGGC
GGACGTCGTTTTTTTGCGTTACCTCGCCCCCCCCGTCCCCACTCCCCCTTCCCCCCCCTT
TTT
Restriction Sites NotI-NotI     
ACCN NM_013352
Insert Size 4880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013352.1, NP_037484.1
RefSeq Size 3998 bp
RefSeq ORF 2877 bp
Locus ID 29940
UniProt ID Q9UL01
Protein Families Transmembrane
Protein Pathways Chondroitin sulfate biosynthesis
Gene Summary The protein encoded by this gene is a tumor-rejection antigen. It is localized to the endoplasmic reticulum and functions to convert D-glucuronic acid to L-iduronic acid during the biosynthesis of dermatan sulfate. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. Mutations in this gene cause inmusculocontractural Ehlers-Danlos syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9, and a paralogous gene exists on chromosome 18. [provided by RefSeq, Apr 2016]
Transcript Variant: This variant (1) encodes isoform a. Variants 1, 2, 3 and 4 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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