SART2 (DSE) (NM_013352) Human Untagged Clone
CAT#: SC115299
DSE (untagged)-Human dermatan sulfate epimerase (DSE), transcript variant 1
CNY 7,024.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DS-epi1; DSEP; DSEPI; EDSMC2; SART-2; SART2 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC115299 sequence for NM_013352 edited (data generated by NextGen Sequencing)
ATGAGGACTCACACACGGGGGGCTCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTG TCAGCCTACATCACCGACGAGAACCCAGAAGTTATGATTCCCTTCACCAATGCCAACTAC GACAGCCATCCCATGCTGTACTTCTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCT GCCAGCTCGCACGAGCACATTGCAGCCCGCCTCACGGAGGCTGTGCACACGATGCTGTCC AGCCCCTTGGAATACCTCCCTCCCTGGGATCCCAAGGACTACAGTGCCCGCTGGAATGAA ATTTTTGGAAACAACTTGGGTGCCTTGGCAATGTTCTGTGTGCTGTATCCTGAGAACATT GAAGCCCGAGACATGGCCAAAGACTACATGGAGAGGATGGCAGCGCAGCCTAGTTGGTTG GTGAAAGATGCTCCTTGGGATGAGGTCCCGCTTGCTCACTCCCTGGTTGGTTTTGCCACT GCTTATGACTTCTTGTACAACTACCTGAGCAAGACACAACAGGAGAAGTTTCTTGAAGTG ATTGCCAATGCCTCAGGGTATATGTATGAAACTTCATACAGGAGAGGATGGGGATTTCAA TACCTGCACAATCATCAGCCCACCAACTGTATGGCTTTGCTCACGGGAAGCCTAGTCCTG ATGAATCAAGGATATCTTCAAGAAGCCTACTTATGGACCAAACAAGTTCTGACCATCATG GAGAAATCTCTGGTCTTGCTCAGGGAGGTGACGGATGGCTCCCTCTATGAAGGAGTTGCG TATGGCAGCTACACCACTAGATCACTCTTCCAATACATGTTTCTCGTCCAGAGGCACTTC AACATCAACCACTTTGGCCATCCGTGGCTTAAACAACACTTTGCATTTATGTATAGAACC ATCCTGCCAGGGTTTCAAAGGACTGTGGCTATTGCGGACTCAAATTACAACTGGTTTTAT GGTCCAGAAAGCCAATTAGTGTTCCTTGATAAATTTGTCATGCGTAATGGCAGTGGTAAC TGGCTAGCTGACCAAATCAGAAGGAACCGTGTGGTGGAAGGTCCAGGAACACCATCCAAA GGGCAGCGCTGGTGCACTCTGCACACAGAATTTCTCTGGTATGATGGCAGCTTGAAATCG GTTCCTCCTCCAGACTTTGGCACCCCTACACTGCATTATTTTGAAGACTGGGGTGTCGTG ACTTATGGAAGTGCACTACCTGCAGAAATCAATAGATCTTTCCTTTCCTTCAAGTCTGGA AAACTGGGGGGACGTGCAATATATGACATTGTCCACAGAAACAAATACAAAGATTGGATC AAAGGATGGAGAAATTTTAATGCAGGGCATGAACATCCTGATCAAAACTCATTTACTTTT NCTCCCAATGGTGTGCCTTTCATTACTGAGGCTCTGTACGGGCCAAAGTACACCTTCTTC AACAATGTTTTGATGTTTTCCCCAGCTGTGTCAAAGAGCTGCTTTTCTCCCTGGGTGGGT CAGGTCACAGAAGACTGCTCATCAAAATGGTCTAAATACAAGCATGACCTGGCAGCTAGT TGTCAGGGGAGGGTGGTTGCAGCAGAGGAGAAAAATGGGGTGGTTTTCATCCGAGGAGAA GGTGTGGGAGCTTATAACCCCCAGCTCAACCTGAAGAATGTTCAGAGGAATCTCATCCTC CTACATCCACAGCTGCTTCTCCTTGTAGATCAAATACACCTGGGAGAGGAGAGTCCCTTG GAGACAGCAGCGAGCTTCTTCCATAATGTGGATGTTCCTTTTGAGGAGACTGTGGTAGAT GGTGTCCATGGGGCTTTCATCAGGCAGAGAGATGGTCTCTATAAAATGTACTGGATGGAC GATACTGGCTACAGCGAGAAAGCAACCTTTGCCTCAGTGACATATCCTCGGGGCTATCCC TACAACGGGACAAACTATGTGAATGTCACCATGCACCTCCGAAGTCCCATCACCAGGGCA GCTTACCTCTTCATAGGGCCATCTATAGATGTTCAGAGCTTCACTGTCCACGGAGACTCT CAGCAACTGGATGTGTTCATAGCCACCAGCAAACATGCCTACGCCACATACCTGTGGACA GGTGAGGCCACAGGACAGTCTGCCTTTGCACAGGTCATTGCTGATCGTCACAAAATTCTG TTTGACCGGAATTCAGCCATCAAGAGCAGCATTGTCCCTGAGGTGAAGGACTATGCTGCT ATTGTGGAACAGAACTTGCAGCATTTTAAACCAGTGTTTCAGCTGCTGGAGAAGCAGATA CTGTCCCGAGTCCGGAACACAGCTAGCTTTAGGAAGACTGCTGAACGCCTGCTGAGATTT TCAGATAAGAGACAGACTGAGGAGGCCATTGACAGGATTTTTGCCATATCACAGCAACAG CAGCAGCAAAGCAAGTCAAAGAAAAACCGAAGGGCAGGCAAACGCTATAAATTTGTGGAT GCTGTCCCTGATATTTTTGCACAGATTGAAGTCAATGAGAAAAAGATTAGACAGAAAGCT CAGATTTTGGCACAGAAAGAACTACCCATAGATGAAGATGAAGAAATGAAAGACCTTTTA GATTTTGCAGATGTAACATACGAGAAACATAAAAATGGGGGCTTGATTAAAGGCCGGTTT GGACAGGCACGGATGGTGACAACTACACACAGCAGGGCCCCATCACTGTCTGCTTCCTAT ACCAGGTTGTTCCTGATTCTGAACATTGCTATTTTCTTTGTCATGTTGGCAATGCAACTG ACTTATTTCCAGAGGGCCCAGAGCCTACATGGCCAAAGATGTCTTTATGCAGTTCTTCTC ATAGATAGCTGTATTTTATTATGGTTGTACTCTTCTTGTTCCCAATCACAGTGTTAG Clone variation with respect to NM_013352.2 1381 g=>n;1710 c=>t >OriGene 5' read for NM_013352 unedited
GCGAATTCGGCACGAGGCATCCCGGGGCATGGCGCGGCGGGGGCGCGGAGGGCTCGGTTC GGAGGGGGCCGGGAGCCCGGGCGCCCTGGAGTGAGGAGGACCGGGAGCTGGCTCTGGAGG CTGCGGAGGCGACGCCGGAGAGAACGAAGCCTCGGCTGGGAGCGGATCTTTCGAAGATGG TTTGGCTGCCTTGGAGATTTGGAGATCTGGTGCCACGATGAGGACTCACACACGGGGGGC TCCCAGTGTGTTTTTCATATATTTGCTTTGCTTTGTGTCAGCCTACATCACCGACGAGAA CCCAGAAGTTATGATTCCCTTCACCAATGCCAACTACGACAGCCATCCCATGCTGTACTT CTCCAGGGCAGAAGTGGCGGAGCTGCAGCTCAGGGCTGCCAGCTCGCACGAGCACATTGC AGCCCGCCTCACGGAGGCTGTGCACACGATGCTGTCCAGCCCCTTGGAATACCTCCCTCC CTGGGATCCCAAGGACTACAGTGCCCGCTGGAATGAAATTTTTGGAAACAACTTGGGTGC CTTGGCAATGTTCTGTGTGCTGTATCCTGAGAACATTGAAGCCCGAGACATGGCCAAAGA CTACATGGAGAGGATGGCAGCGCAACCTAGTTGGTTGGGAAAGATGCTCCTTGGGATGAG GTCCCGCTTGCTCACTCCCTGGGTGGTTTTGCCACTGCTTATGACTTCTTNGTACACTAC CTGAGNCAGACACACAGGAGAAGTTTCTTG >OriGene 3' read for NM_013352 unedited
TTTGTACCGAGCGTCCNGTCCAGGATCGAGATATATCTTTTTTTTTTTNGAAAATTCACA ATTATGATGTTTATTTAGGGAAAATAAACTTGAAGAAGGTTTTCTATTAAAAAAACATAA GAATCATATCCAAATTTTAGACTCTCCATCTTCTTTGGTATGAAAATCAACATTCATAAT AGAAAATATTTTCTTTTGAAAGGGTTAGTTGGGAGTGGGTCTATAACGGTTTTCCATATA AGGAAAATACAGTATTATCTGTAATCATCTCACTTATTGAACAACCAATGAATGCATGCA AGTAACCAAGCATATGTGTGCAGAGACCAGCATCAAGGAAATATTCATTCATAGATAAAT GTTTTCTAATAAAATATATTTTAAGTTTTTAATGATCTTCTGCTATCATTTTTTGCCATG TTATCTGAATAGCCATCCAGATCTGTAGTAAAACATCCTGTTAGTTACATAACTCAAGAA GTACTTCCCCAAGGAAGCTCACACCATCATACCAGGACTTAGCAACATTTATCTTTCAAC ATGAGTATACCATTAAAATTTTTTTATATTTCTCAGAATTGCATTCTTCAGTCTTTTCCC CATATAGTCTATGGAAATTGCTAATACCATTAAAATAATATTAATAAAACCCAATTCTAT CTCAACCTTTTTCTCATAAGACAAAACCTTACTTTTAACAATAGACTTTCTGGTTCTTCT CCACAGTCTCCTCTACGGACCGCTCACTGCGCATGCCCAATCCTTTCAAAACCCCCTCGG CCCCCACGCTATTGCCACCGCTTGTTCCCCCTTTTTCTCCCCCTTCTTTCCCCGCCCGTT TCTTGCGCCGTATCCCTCCTTTTTTTCGGCCCCGACGCGGCGGGCCACTCTGTTCCTGGC GGACGTCGTTTTTTTGCGTTACCTCGCCCCCCCCGTCCCCACTCCCCCTTCCCCCCCCTT TTT |
Restriction Sites | NotI-NotI |
ACCN | NM_013352 |
Insert Size | 4880 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013352.1, NP_037484.1 |
RefSeq Size | 3998 bp |
RefSeq ORF | 2877 bp |
Locus ID | 29940 |
UniProt ID | Q9UL01 |
Protein Families | Transmembrane |
Protein Pathways | Chondroitin sulfate biosynthesis |
Gene Summary | The protein encoded by this gene is a tumor-rejection antigen. It is localized to the endoplasmic reticulum and functions to convert D-glucuronic acid to L-iduronic acid during the biosynthesis of dermatan sulfate. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. Mutations in this gene cause inmusculocontractural Ehlers-Danlos syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9, and a paralogous gene exists on chromosome 18. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) encodes isoform a. Variants 1, 2, 3 and 4 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206020 | DSE (Myc-DDK-tagged)-Human dermatan sulfate epimerase (DSE), transcript variant 1 |
CNY 7,016.00 |
|
RC206020L3 | Lenti ORF clone of Human dermatan sulfate epimerase (DSE), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206020L4 | Lenti ORF clone of Human dermatan sulfate epimerase (DSE), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG206020 | DSE (tGFP-tagged) - Human dermatan sulfate epimerase (DSE), transcript variant 1 |
CNY 8,616.00 |